Dowell, Simon J., Tsang, Jimmy S.H., Mellor, Jane
CPF1 is a basic helix-loop-helix (bHLH) protein required for optimal centromere function and for maintaining methionine independent growth in yeast. In this work, we show that the region...
Characterisation of the DNA binding domain of the yeast RAP1 protein (1990)
Henry, Yves A.L., Chambers, Alistair, Tsang, Jimmy S.H., Kingsman, Alan J., Kingsman, Susan M.
The 827 amino acid yeast RAP1 protein interacts with DNA to regulate gene expression at numerous unrelated loci in the yeast genome. By a combination of amino, carboxy and internal deletions, we have...
Chambers, Alistair, Stanway, Clive, Tsang, Jimmy S.H., Henry, Yves, Kingsman, Alan J., Kingsman, Susan M.
The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the...
Tsang, Jimmy S.H., Henry, Yves A.L., Chambers, Alistair, Kingsman, Alan J., Kingsman, Susan M.
Yeast repressor activator protein 1 (RAP 1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires...
Identification of the Dimerization Domain of Dehalogenase IVa of Burkholderia cepacia MBA4
Tsang, Jimmy S. H., Pang, Benjamin C. M.
Haloacid dehalogenases are enzymes that catalyze the hydrolytic removal of halogens from haloalkanoic acids. Dehalogenase IVa (DehIVa) from Burkholderia cepacia MBA4 and dehalogenase CI (DehCI) from...
Cloning and Characterization of a Cryptic Haloacid Dehalogenase from Burkholderia cepacia MBA4
Tsang, Jimmy S. H., Sam, Laiju
Burkholderia cepacia MBA4 has been shown to produce a single dehalogenase batch culture. Moreover, other cryptic dehalogenases were also detected when the cells were grown in continuous culture. In...
Identification of the Dimerization Domain of Dehalogenase IVa of Burkholderia cepacia MBA4
Tsang, Jimmy S. H., Pang, Benjamin C. M.
Haloacid dehalogenases are enzymes that catalyze the hydrolytic removal of halogens from haloalkanoic acids. Dehalogenase IVa (DehIVa) from Burkholderia cepacia MBA4 and dehalogenase CI (DehCI) from...
Cloning and Characterization of a Cryptic Haloacid Dehalogenase from Burkholderia cepacia MBA4
Tsang, Jimmy S. H., Sam, Laiju
Burkholderia cepacia MBA4 has been shown to produce a single dehalogenase batch culture. Moreover, other cryptic dehalogenases were also detected when the cells were grown in continuous culture. In...
Schmidberger, Jason W., Oakley, Aaron J., Tsang, Jimmy S. H., Wilce, Matthew C. J.
The dhlIVa gene coding for DehIVa was expressed in Escherichia coli and the protein was purified and crystallized using the hanging-drop method.
Isolation and Characterization of a Novel Haloacid Permease from Burkholderia cepacia MBA4▿
Yu, Manda, Faan, Yun-Wing, Chung, Wilson Y. K., Tsang, Jimmy S. H.
Burkholderia cepacia MBA4 is a bacterium that can utilize 2-haloacids as carbon and energy sources for growth. It has been proposed that dehalogenase-associated permease mediates the uptake of...