Van Roy, Nadine, De Preter, Katleen, Hoebeeck, Jasmien, Van Maerken, Tom, Pattyn, Filip, Mestdagh, Pieter, ...
Abstract Neuroblastoma is one of the most common solid tumors of childhood, arising from immature sympathetic nervous system cells. The clinical course of patients with neuroblastoma is highly...
A novel and universal method for microRNA RT-qPCR data normalization (2009)
Mestdagh, Pieter, Van Vlierberghe, Pieter, De Weer, An, Muth, Daniel, Westermann, Frank, Speleman, Frank, ...
Abstract Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample...
Aberrant methylation of candidate tumor suppressor genes in neuroblastoma (2009)
Hoebeeck, Jasmien, MICHELS, EVELIEN, Pattyn, Filip, Combaret, Valerie, Vermeulen, Joelle, Yigit, Nurten, ...
CpG island hypermethylation has been recognized as an alternative mechanism for tumor suppressor gene inactivation. In this study, we performed methylation-specific PCR (MSP) to investigate the...
Lampo, Evelyne, Van Poucke, Mario, Vandesompele, Jo, Erkens, Tim, Van Zeveren, Alex, Peelman, Luc
SPRN is an interesting new member of the PRNP family because of its sequence homology with the hydrophobic region of PRNP, its expression in brain tissue and its PrP-like properties in functional...
Genetic analysis of PRNP, SPRN and RPSA in relation to prion disease resistance in sheep (2009)
Van Poucke, Mario, Lampo, Evelyne, Van Den Broeke, Alice, Wilemarck, Nicolas, Vander Donckt, Dominique, Rogiers, Carolien, ...
RDML: structured language and reporting guidelines for real-time quantitative PCR data (2009)
Lefever, Steve, Hellemans, Jan, Pattyn, Filip, Przybylski, Daniel R., Taylor, Chris, Geurts, René, ...
The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exchange of qPCR data and related information between...
RTPrimerDB: the portal for real-time PCR primers and probes (2009)
Lefever, Steve, Vandesompele, Jo, Speleman, Frank, Pattyn, Filip
RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked...
Westermann, Frank, Muth, Daniel, Benner, Axel, Bauer, Tobias, Henrich, Kai-Oliver, Oberthuer, André, ...
Abstract Background Amplified MYCN oncogene resulting in deregulated MYCN transcriptional activity is observed in 20% of neuroblastomas and identifies a highly aggressive subtype. In MYCN single-copy...
Taylor, Chris F., Field, Dawn, Sansone, Susanna-Assunta, Aerts, Jan, Apweiler, Rolf, Asburner, Michael, ...
The Minimum Information for Biological and Biomedical Investigations (MIBBI) project provides a resource for those exploring the range of extant minimum information checklists and fosters coordinated...
Michels, Evi, Hoebeeck, Jasmien, De Preter, Katleen, Schramm, Alexander, Brichard, Bénédicte, De Paepe, Anne, ...
Abstract Background Recurrent loss of part of the long arm of chromosome 11 is a well established hallmark of a subtype of aggressive neuroblastomas. Despite intensive mapping efforts to localize the...
Bmc Bioinformatics, Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Evi Michels, Anne De, ...
Research article methBLAST and methPrimerDB: web-tools for PCR based methylation analysis
Hoogewijs, David, Houthoofd, Koen, Matthijssens, Filip, Vandesompele, Jo, Vanfleteren, Jacques R
Abstract Background In the nematode Caenorhabditis elegans the conserved Ins/IGF-1 signaling pathway regulates many biological processes including life span, stress response, dauer diapause and...
Unmasking of epigenetically silenced micromas in neuroblastoma (2008)
Hoebeeck, Jasmien, Declerck, Geert, Mestdagh, Pieter, Pattyn, Filip, De Preter, Katleen, Vermeulen, Joëlle, ...
Unmasking of epigenetically silenced micromas in neuroblastoma (2008)
Hoebeeck, Jasmien, Declerck, Geert, Mestdagh, Pieter, Pattyn, Filip, De Preter, Katleen, Vermeulen, Joëlle, ...
De Preter, Katleen, Menten, Björn, De Brouwer, Sara, Kumps, Candy, Michels, Evi, Van Roy, Nadine, ...
Van Roy, Frans, Vandepoele, Karl, Van Roy, Nadine, Andries, Vanessa, Staes, Katrien, Vandesompele, Jo, ...
Mestdagh, Pieter, Feys, Tom, Bernard, Nathalie, Guenther, Simone, Chen, Caifu, Speleman, Franki, ...
MICRORNA PROFILING OF EVI1 DEREGULATED MYELOID LEUKEMIA (2008)
De Weer, An, Poppe, Bruce, Mestdagh, Pieter, Van Vlierberghe, Pieter, Van Roy, Nadine, De Paepe, Anne, ...
Taylor, CF, Field, D, Sansone, SA, Aerts, J, Apweiler, R, Ashburner, M, ...
Standardization of real-time PCR gene expression data from independent biological replicates (2008)
Willems, Erik, Leyns, Luc, Vandesompele, Jo
Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different...
Westermann, F, Muth, D, Benner, A, Bauer, T, Henrich, KO, Oberthuer, A, ...
Background Amplified MYCN oncogene resulting in deregulated MYCN transcriptional activity is observed in 20% of neuroblastomas and identifies a highly aggressive subtype. In MYCN single-copy...
Vandepoele, Karl, Andries, Vanessa, Van Roy, Nadine, Staes, Katrien, Vandesompele, Jo, Laureys, Genevieve, ...
Real-time qPCR as a tool for evaluating RNAi-mediated gene silencing (2008)
Van Maerken, Tom, Mestdagh, Pieter, De Clercq, Sarah, Pattyn, Filip, Yigit, Nurten, De Paepe, Anne, ...
This article describes how rt-qPCR can be implemented as a tool to monitor silencing efficiency and functional effects of RNA interference (RNAi)-mediated gene knockdown, using examples from our...
De Preter, Katleen, Barriot, Roland, Speleman, Frank, Vandesompele, Jo, Moreau, Yves
The search for feature enrichment is a widely used method to characterize a set of genes. While several tools have been designed for nominal features such as Gene Ontology annotations or KEGG...
Mestdagh, Pieter, Feys, Tom, Bernard, Nathalie, Guenther, Simone, Chen, Caifu, Speleman, Frank, ...
MicroRNAs (miRNAs) are an emerging class of small non-coding RNAs implicated in a wide variety of cellular processes. Research in this field is accelerating, and the growing number of miRNAs...
Molecular dissection of isolated disease features in mosaic neurofibromatosis type 1 (2007)
De Schepper, Sofie, Vandesompele, Jo, Heyns, Ine, Janssens, Sandra, ...
Elucidation of the biological framework underlying the development of neurofibromatosis type 1 (NF1)-related symptoms has proved to be difficult. Complicating factors include the large size of the...
Identification and expression analysis of genes associated with bovine blastocyst formation (2007)
Goossens, Karen, Van Soom, Ann, Van Poucke, Mario, Vandaele, Leen, Vandesompele, Jo, Van Zeveren, Alex, ...
Abstract Background Normal preimplantation embryo development encompasses a series of events including first cleavage division, activation of the embryonic genome, compaction and blastocyst...
Hellemans, Jan, Mortier, Geert, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo
Abstract Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major...
De Preter, Katleen, Vandesompele, Jo, Heimann, Pierre, Yigit, Nurten, Beckman, Siv, Schramm, Alexander, ...
No abstract available.
Identification and expression analysis of genes associated with bovine blastocyst formation (2007)
Goossens, Karen, Van Soom, Ann, Van Poucke, Mario, Vandaele, Leen, Vandesompele, Jo, Van Zeveren, Alex, ...
Hoebeeck, Jasmien, Ongenaert, Maté, Michels, Evi, De Preter, Katleen, Vermeulen, Joëlle, Yigit, Nurten, ...
ArrayCGH-based classification of neuroblastoma into genomic subgroups (2007)
Michels, Evi, Vandesompele, Jo, De Preter, Katleen, Hoebeeck, Jasmien, Vermeulen, Joëlle, SCHRAMM, A, ...
Integrative genomics in neuroblastoma research (2007)
Speleman, Franki, De Preter, Katleen, Michels, Evi, Hoebeeck, Jasmien, Pattyn, Filip, VERTMEULEN, J, ...
ArrayCGH based classification of neuroblastoma into genomic subgroups (2007)
De Brouwer, Sara, Michels, Evi, Vandesompele, Jo, De Preter, Katleen, Hoebeeck, Jasmien, Vermeulen, Joëlle, ...
New insights into the genetic basis of neuroblastoma (2007)
Speleman, Franki, De Preter, Katleen, Hoebeeck, Jasmien, Van Roy, Nadine, Vandesompele, Jo
MicroRNA profiling of EVI1 deregulated myeloid leukemia (2007)
De Weer, An, Poppe, Bruce, Mestdagh, Pieter, Van Roy, Nadine, De Paepe, Anne, Verhasselt, Bruno, ...
Molecular dissection of isolated disease features in mosaic neurofibromatosis type 1 (2007)
Maertens, Ophélia, DE SCHEPPER, S, Vandesompele, Jo, BRERNS, H, HEYNS, I, JANSSENS, SANDRA, ...
Real-time PCR quality control for gene expression profiling on the LightCycler 480 System (2007)
Hoebeeck, Jasmien, Pattyn, Filip, NURTEN, Y, DE SMET, E, Speleman, Franki, Vandesompele, Jo
Array CGH based classification of neuroblastoma into genomic subgroups. (2007)
De Brouwer, Sara, Michels, Evi, Vandesompele, Jo, De Preter, Katleen, Hoebeeck, Jasmien, VERMEULEN, J, ...
Menten, Björn, Buysse, Karen, Vermeulen, Stefan, Meersschaut, Valerie, Vandesompele, Jo, NG, BL, ...
Rapid and easy prenatal diagnosis of sickle cell anemia using double-dye LNA probe technology (2007)
Housni, Hakim E., Vandesompele, Jo, Vannuffel, Pascal, Gulbis, Beatrice, Parma, Jasmine, Cochaux, Pascale
Speleman, Frank, Messiaen, Ludwine, Vandesompele, Jo
Center for Medical Genetics, Ghent University Hospital, B-9000 Ghent, Belgium.
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis (2006)
Pattyn, Filip, Hoebeeck, Jasmien, Robbrecht, Piet, Michels, Evi, De Paepe, Anne, Bottu, Guy, ...
Abstract Background DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methylation...
Erkens, Tim, Van Poucke, Mario, Vandesompele, Jo, Goossens, Karen, Van Zeveren, Alex, Peelman, Luc J
Abstract Background An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be drawn. This can be done by using one or multiple,...
Comprehensive NF1 screening on cultured Schwann cells from neurofibromas (2006)
Vandesompele, Jo, Heyns, Ine, Rosenbaum, Thorsten, ...
Neurofibromatosis type 1 (NF1) is mainly characterized by the occurrence of benign peripheral nerve sheath tumors or neurofibromas. Thorough investigation of the somatic mutation spectrum has thus...
De Preter, Katleen, Vandesompele, Jo, Heimann, Pierre, Yigit, Nurten, Beckman, Siv, Schramm, Alexander, ...
Abstract Background Neuroblastoma tumor cells are assumed to originate from primitive neuroblasts giving rise to the sympathetic nervous system. Because these precursor cells are not detectable in...
Dastugue, Nicole, Vandesompele, Jo, Cauwelier, Barbara, Yigit, Nurten, ...
In contrast to the well-documented involvement of EVI1 in various 3q26 aberrations, the transcriptional status of EVI1 in rare recurrent or sporadic 3q26 chromosomal defects has remained largely...
Swerts, Katrien, De Moerloose, Barbara, Dhooge, Catharina, Vandesompele, Jo, HOYOUX, C, BEISKE, K, ...
The potential (radio-)biological impact of launch vibration (2006)
BAERT, P, VAN CLEYNENBREUGEL, T, Vandesompele, Jo, De Schynkel, Sofie, VANDER SLOTEN, J, Van Oostveldt, Patric
PPARGC1A mRNA expression in backfat and longissimus dorsi muscle in the pig (2006)
Erkens, Tim, Van Poucke, Mario, Vandesompele, Jo, Goossens, Karen, Van Zeveren, Alex, Peelman, Luc
Differential prion protein mRNA expression in sheep blood (2006)
Van Poucke, Mario, Vandesompele, Jo, De Ketelaere, Adelheid, Goossens, Karen, Mattheeuws, Marc, Van Zeveren, Alex, ...
PRNP polymorphisms in sheep with differential PRNP mRNA expression in blood (2006)
Van Poucke, Mario, Vandesompele, Jo, De Ketelaere, Adelheid, Goossens, Karen, Van Zeveren, Alex, Peelman, Luc
Comprehensive NF1 screening on cultured Schwann cells from neurofibromas (2006)
Maertens, Ophélia, BREMS, H, Vandesompele, Jo, DE RAEDT, T, HEYNS, I, ROSENBAUM, T, ...
Murine m cells express annexin V specifically (2006)
Verbrugghe, Phebe, Waelput, Wim, Dieriks, Birger, Waeytens, Anouk, Vandesompele, Jo, Cuvelier, Claude
Hoebeeck, Jasmien, Vandesompele, Jo, NILSSON, H, De Preter, Katleen, Van Roy, Nadine, De Smet, Els, ...
RTPrimerDB: the real-time PCR primer and probe database, major update 2006 (2006)
Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Franki, Vandesompele, Jo
Small-molecule MDM2 antagonists as a new therapy concept for neuroblastoma (2006)
Van Maerken, Tom, Speleman, Franki, VERMEULEN, J, LAMBERTZ, I, DE CLERCQ, S, De Smet, Els, ...
MethBLAST and methPrimerDB: web-tools for PCR based methylation analysis (2006)
Pattyn, Filip, Hoebeeck, Jasmien, Robbrecht, Piet, Michels, Evi, De Paepe, Anne, BOTTU, G, ...
Michels, Evi, Vandesompele, Jo, Hoebeeck, Jasmien, Menten, Björn, De Preter, Katleen, Laureys, Genevieve, ...
Maertens, Ophélia, Brems, Hilde, Vandesompele, Jo, De Raedt, Thomas, Heyns, Ine, Rosenbaum, Thorsten, ...
Neurofibromatosis type 1 (NF1) is mainly characterized by the occurrence of benign peripheral nerve sheath tumors or neurofibromas. Thorough investigation of the somatic mutation spectrum has thus...
RTPrimerDB: the real-time PCR primer and probe database, major update 2006 (2006)
Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo
The RTPrimerDB (http://medgen.ugent.be/rtprimerdb) project provides a freely accessible data retrieval system and an in silico assay evaluation pipeline for real-time quantitative PCR assays. Over...
Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos (2005)
Goossens, Karen, Van Poucke, Mario, Van Soom, Ann, Vandesompele, Jo, Van Zeveren, Alex, Peelman, Luc J
Abstract Background Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, in order to gain information about embryo...
Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11 (2005)
De Preter, Katleen, Vandesompele, Jo, Menten, Björn, Carr, Philippa, Fiegler, Heike, Edsjö, Anders, ...
Abstract Background Loss of chromosome 11q defines a subset of high-stage aggressive neuroblastomas. Deletions are typically large and mapping efforts have thus far not lead to a well defined...
arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays (2005)
Menten, Björn, Pattyn, Filip, De Preter, Katleen, Robbrecht, Piet, Michels, Evi, Buysse, Karen, ...
Abstract Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered...
arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays (2005)
Pattyn, Filip, De Preter, Katleen, Robbrecht, Piet, Michels, Evi, Buysse, Karen, ...
\emph{BMC Bioinformatics}, vol. 6, no. 124, May 2005
A dual fluorescent multiprobe assay for prion protein genotyping in sheep (2005)
Van Poucke, Mario, Vandesompele, Jo, Mattheeuws, Marc, Van Zeveren, Alex, Peelman, Luc J
Abstract Background Scrapie and BSE belong to a group of fatal, transmissible, neurodegenerative diseases called TSE. In order to minimize the risk of natural scrapie and presumed natural BSE in...
arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays (2005)
Menten, Björn, Pattyn, Filip, De Preter, Katleen, Robbrecht, Piet, Michels, Evi, Buysse, Karen, ...
A dual fluorescent multiprobe assay for prion protein genotyping in sheep (2005)
Van Poucke, Mario, Vandesompele, Jo, Mattheeuws, Marc, Van Zeveren, Alex, Peelman, Luc
Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos (2005)
Goossens, Karen, Van Poucke, Mario, Van Soom, Ann, Vandesompele, Jo, Van Zeveren, Alex, Peelman, Luc
Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11 (2005)
De Preter, Katleen, Vandesompele, Jo, Menten, Björn, CARR, P, FIEGLER, H, EDSJO, A, ...
Rapid detection of VHL exon deletions using real-time quantitative PCR (2005)
Hoebeeck, Jasmien, Poppe, Bruce, De Smet, Els, Yigit, Nurten, Claes, Kathleen, ...
A dual fluorescent multiprobe assay for prion protein genotyping in sheep (2005)
Van Poucke, Mario, Vandesompele, Jo, Mattheeuws, Marc, Van Zeveren, Alex, Peelman, Luc
Impact of RNA quality on reference gene expression stability (2005)
PEREZ-NOVO, CA, Claeys, Cindy, Speleman, Franki, Van Cauwenberge, Paul, Bachert, Claus, Vandesompele, Jo
Hellemans, Jan, Preobrazhenska, Olena; U0041254, Willaert, Andy, Debeer, Philippe; U0002219, Verdonk, Peter C M, Costa, Teresa, ...
Osteopoikilosis, Buschke-Ollendorff syndrome (BOS) and melorheostosis are disorders characterized by increased bone density. The occurrence of one or more of these phenotypes in the same individual...
No evidence for involvement of SDHDin neuroblastoma pathogenesis (2004)
De Preter, Katleen, Vandesompele, Jo, Hoebeeck, Jasmien, Vandenbroecke, Caroline, Smet, Jöel, Nuyts, Annick, ...
Abstract Background Deletions in the long arm of chromosome 11 are observed in a subgroup of advanced stage neuroblastomas with poor outcome. The deleted region harbours the tumour suppressor gene...
De Preter, Katleen, Pattyn, Filip, Berx, Geert, Strumane, Kristin, Menten, Björn, Van Roy, Frans, ...
Abstract Background Activation of proto-oncogenes by DNA amplification is an important mechanism in the development and maintenance of cancer cells. Until recently, identification of the targeted...
Edsjö, Anders, Nilsson, Helén, Vandesompele, Jo, Karlsson, Jenny, Pattyn, Filip, Culp, Lloyd A, ...
Abstract is not available
De Preter, Katleen, Pattyn, Filip, Berx, Geert, Strumane, Kristin, Menten, Björn, Van Roy, Frans, ...
The human FOXL2 mutation database (2004)
Beysen, Diane, Vandesompele, Jo, MESSIAEN, L, De Paepe, Anne, De Baere, Elfride
No evidence for involvement of SDHD in neuroblastoma pathogenesis (2004)
De Preter, Katleen, Vandesompele, Jo, Hoebeeck, Jasmien, VANDENBROECKE, C, Smet, Joél, NUYTS, ANN, ...
Gele, Mireille Van, Boyle, Glen M, Cook, Anthony, Vandesompele, Jo, Boonefaes, Tom, Rottiers, Pieter, ...
Merkel cell carcinoma (MCC) is a rare aggressive skin tumor which shares histopathological and genetic features with small-cell lung carcinoma (SCLC), both are of neuroendocrine origin. Comparable to...
Gele, Mireille Van, Boyle, Glen M, Cook, Anthony, Vandesompele, Jo, Boonefaes, Tom, Rottiers, Pieter, ...
Merkel cell carcinoma (MCC) is a rare aggressive skin tumor which shares histopathological and genetic features with small-cell lung carcinoma (SCLC), both are of neuroendocrine origin. Comparable to...
Pérez, Claudina, Vandesompele, Jo, Vandenbroucke, Ina, Holtappels, Gabriele, Speleman, Frank, Gevaert, Philippe, ...
Abstract Background Expression of human Interleukin-5 receptor alpha (hIL-5Rα) is controlled by alternative splicing, which generates two different transcripts encoding a membrane-anchored and a...
Van Gele, Mireille, Vandesompele, Jo, Messiaen, Ludwine, Van Belle, Simon, ...
Malignant peripheral nerve sheath tumors (MPNST) are rare soft-tissue malignancies. The genetic basis of these tumors is still poorly understood. Cytogenetic analyses predominantly revealed complex...
ID2 expression in neuroblastoma does not correlate to MYCN levels and lacks prognostic value (2003)
Vandesompele, Jo, EDSJO, A, De Preter, Katleen, AXELSON, H, Speleman, Franki, PAHLMAN, S
Van Roy, Nadine, VAN GELE, M, Vandesompele, Jo, Messiaen, Ludwine, Van Belle, Simon, MORTELE, K, ...
Beysen, Diane, Vandesompele, Jo, Pattyn, Filip, De Paepe, Anne, Messiaen, Ludwine, De Baere, Elfride
Regulation of peroxisomal genes by DHEA and vitamin D (2003)
DEPRETER, M, Vandesompele, Jo, FERDINANDUSSE, S, Speleman, Franki, Roels, Frank
Regulation of peroxisomal genes by DHEA and vitamin D (2003)
DEPRETER, M, Vandesompele, Jo, FERDINANDUSSE, S, Speleman, Franki, Roels, Frank
Perez Novo, Claudina, Vandesompele, Jo, Holtappels, Gabriële, Speleman, Franki, Gevaert, Philippe, Van Cauwenberge, Paul, ...
RTPrimerDB: The Real-Time PCR primer and probe database (2003)
Pattyn, Filip, Speleman, Franki, De Paepe, Anne, Vandesompele, Jo
Bmc Genomics, Katleen De Preter, Filip Pattyn, Geert Berx, Kristin Strumane, Björn Menten, ...
Research article Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons
RTPrimerDB: the Real-Time PCR primer and probe database (2003)
Pattyn, Filip, Speleman, Frank, De Paepe, Anne, Vandesompele, Jo
The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation...
Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...
Abstract Background Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and...
Diss. doct. geneeskunde
Quantification of NF1 transcripts reveals novel highly expressed splice variants. (2002)
Vandenbroucke, Ina, Vandesompele, Jo, De Paepe, Anne, Messiaen, Ludwine
Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...
De Paepe, Anne, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Speleman, Franki, Van Roy, Nadine, ...
Quantification of soluble and membrane anchored isoforms of IL-5R alpha using real-time PCR (2002)
NOVO, CP, Vandesompele, Jo, VANDENBROUCKE, I, HOLTAPPELS, G, Gevaert, Philippe, Van Cauwenberge, Paul, ...
Vandesompele, Jo, Speleman, Frank, Van Roy, Nadine, Laureys, Geneviève, Brinskchmidt, Christian, Christiansen, Holger, ...
Procedure: Analysis of comparative genomic hybridization (CGH) data of 120 tumors from four different studies, and data of 84 previously unpublished tumors, allowed delineation of at least six...
Vandesompele, Jo, Speleman, Frank, Van Roy, Nadine, Laureys, Genevieve, Brinskchmidt, Christian, Christiansen, Holger, ...
Procedure Analysis of comparative genomic hybridization (CGH) data of 120 tumors from four different studies, and data of 84 previously unpublished tumors, allowed delineation of at least six...
Vandesompele, Jo, Speleman, Franki, Van Roy, Nadine, Laureys, Genevieve, BRINKSCHMIDT, C, CHRISTIANSEN, H, ...
Van Roy, Nadine, Van Limbergen, Heidi, Vandesompele, Jo, Van Gele, Mireille, Poppe, Bruce, SALWEN, H, ...
Quantification of splice variants using real-time PCR (2001)
Vandenbroucke, Ina I., Vandesompele, Jo, Paepe, Anne De, Messiaen, Ludwine
A reliable and robust method for measuring the expression of alternatively spliced transcripts is an important step in investigating the significance of each variant. So far, accurate quantification...
Van Roy, Nadine, Van Limbergen, Heidi, Vandesompele, Jo, Van Gele, Mireille, Poppe, Bruce, Laureys, Genevieve, ...
Neuroblastoma: from molecular cytogenetics to gene expression profiling (1999)
Vandesompele, Jo, Van Roy, Nadine, Berx, Geert, PLANTAZ, D, De Paepe, Anne, Laureys, Genevieve, ...
Molecular analysis of lp36 breakpoints in two Merkel cell carcinomas. (1998)
Van Gele, Mireille, Van Roy, Nadine, RONAN, SG, Messiaen, Ludwine, Vandesompele, Jo, Geerts, Maria, ...
Genetic heterogeneity of neuroblastoma studied by comparative genomic hybridization. (1998)
Vandesompele, Jo, Van Roy, Nadine, Van Gele, Mireille, Laureys, Genevieve, AMBROS, P, HEIMANN, P, ...
Quantification of splice variants using real-time PCR
Vandenbroucke, Ina I., Vandesompele, Jo, Paepe, Anne De, Messiaen, Ludwine
A reliable and robust method for measuring the expression of alternatively spliced transcripts is an important step in investigating the significance of each variant. So far, accurate quantification...
Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for...
RTPrimerDB: the Real-Time PCR primer and probe database
Pattyn, Filip, Speleman, Frank, De Paepe, Anne, Vandesompele, Jo
The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation is...
Pérez, Claudina, Vandesompele, Jo, Vandenbroucke, Ina, Holtappels, Gabriele, Speleman, Frank, Gevaert, Philippe, ...
De Preter, Katleen, Pattyn, Filip, Berx, Geert, Strumane, Kristin, Menten, Björn, Van Roy, Frans, ...
No evidence for involvement of SDHD in neuroblastoma pathogenesis
De Preter, Katleen, Vandesompele, Jo, Hoebeeck, Jasmien, Vandenbroecke, Caroline, Smet, Jöel, Nuyts, Annick, ...
arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays
Menten, Björn, Pattyn, Filip, De Preter, Katleen, Robbrecht, Piet, Michels, Evi, Buysse, Karen, ...
Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11
De Preter, Katleen, Vandesompele, Jo, Menten, Björn, Carr, Philippa, Fiegler, Heike, Edsjö, Anders, ...
A dual fluorescent multiprobe assay for prion protein genotyping in sheep
Van Poucke, Mario, Vandesompele, Jo, Mattheeuws, Marc, Van Zeveren, Alex, Peelman, Luc J
Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
Goossens, Karen, Van Poucke, Mario, Van Soom, Ann, Vandesompele, Jo, Van Zeveren, Alex, Peelman, Luc J
RTPrimerDB: the real-time PCR primer and probe database, major update 2006
Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo
The RTPrimerDB () project provides a freely accessible data retrieval system and an in silico assay evaluation pipeline for real-time quantitative PCR assays. Over the last year the number of user...
Erkens, Tim, Van Poucke, Mario, Vandesompele, Jo, Goossens, Karen, Van Zeveren, Alex, Peelman, Luc J
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis
Pattyn, Filip, Hoebeeck, Jasmien, Robbrecht, Piet, Michels, Evi, De Paepe, Anne, Bottu, Guy, ...
Quantification of splice variants using real-time PCR
Vandenbroucke, Ina I., Vandesompele, Jo, Paepe, Anne De, Messiaen, Ludwine
A reliable and robust method for measuring the expression of alternatively spliced transcripts is an important step in investigating the significance of each variant. So far, accurate quantification...
Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for...
RTPrimerDB: the Real-Time PCR primer and probe database
Pattyn, Filip, Speleman, Frank, De Paepe, Anne, Vandesompele, Jo
The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation is...
Pérez, Claudina, Vandesompele, Jo, Vandenbroucke, Ina, Holtappels, Gabriele, Speleman, Frank, Gevaert, Philippe, ...
De Preter, Katleen, Pattyn, Filip, Berx, Geert, Strumane, Kristin, Menten, Björn, Van Roy, Frans, ...
No evidence for involvement of SDHD in neuroblastoma pathogenesis
De Preter, Katleen, Vandesompele, Jo, Hoebeeck, Jasmien, Vandenbroecke, Caroline, Smet, Jöel, Nuyts, Annick, ...
arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays
Menten, Björn, Pattyn, Filip, De Preter, Katleen, Robbrecht, Piet, Michels, Evi, Buysse, Karen, ...
Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11
De Preter, Katleen, Vandesompele, Jo, Menten, Björn, Carr, Philippa, Fiegler, Heike, Edsjö, Anders, ...
A dual fluorescent multiprobe assay for prion protein genotyping in sheep
Van Poucke, Mario, Vandesompele, Jo, Mattheeuws, Marc, Van Zeveren, Alex, Peelman, Luc J
Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
Goossens, Karen, Van Poucke, Mario, Van Soom, Ann, Vandesompele, Jo, Van Zeveren, Alex, Peelman, Luc J
RTPrimerDB: the real-time PCR primer and probe database, major update 2006
Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo
The RTPrimerDB () project provides a freely accessible data retrieval system and an in silico assay evaluation pipeline for real-time quantitative PCR assays. Over the last year the number of user...
Erkens, Tim, Van Poucke, Mario, Vandesompele, Jo, Goossens, Karen, Van Zeveren, Alex, Peelman, Luc J
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis
Pattyn, Filip, Hoebeeck, Jasmien, Robbrecht, Piet, Michels, Evi, De Paepe, Anne, Bottu, Guy, ...
De Preter, Katleen, Vandesompele, Jo, Heimann, Pierre, Yigit, Nurten, Beckman, Siv, Schramm, Alexander, ...
Transcriptome profiling of neuroblasts and neuroblastoma tumor cells provides strong support for a neuroblast origin of neuroblastoma and highlights new candidate neuroblastoma genes
De Preter, Katleen, Vandesompele, Jo, Heimann, Pierre, Yigit, Nurten, Beckman, Siv, Schramm, Alexander, ...
A correction to Human fetal neuroblast and neuroblastoma transcriptome analysis confirms neuroblast origin and highlights neuroblastoma candidate genes by K De Preter, J Vandesompele, P Heimann, N...
Hellemans, Jan, Mortier, Geert, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo
qBase, a free program for the management and automated analysis of qPCR data, is described
Identification and expression analysis of genes associated with bovine blastocyst formation
Goossens, Karen, Van Soom, Ann, Van Poucke, Mario, Vandaele, Leen, Vandesompele, Jo, Van Zeveren, Alex, ...
De Preter, Katleen, Barriot, Roland, Speleman, Frank, Vandesompele, Jo, Moreau, Yves
The search for feature enrichment is a widely used method to characterize a set of genes. While several tools have been designed for nominal features such as Gene Ontology annotations or KEGG...
Vandepoele, Karl, Andries, Vanessa, Van Roy, Nadine, Staes, Katrien, Vandesompele, Jo, Laureys, Geneviève, ...
The human 1p36 region is deleted in many different types of tumors, and so it probably harbors one or more tumor suppressor genes. In a Belgian neuroblastoma patient, a constitutional balanced...
Michels, Evi, Hoebeeck, Jasmien, De Preter, Katleen, Schramm, Alexander, Brichard, Bénédicte, De Paepe, Anne, ...
Quantification of splice variants using real-time PCR. Nucleic Acids Res., 29, 2001, E68.
Vandenbroucke, Ina, Vandesompele, Jo, De Paepe, Anne, Messiaen, Ludwine
High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
Mestdagh, Pieter, Feys, Tom, Bernard, Nathalie, Guenther, Simone, Chen, Caifu, Speleman, Frank, ...
MicroRNAs (miRNAs) are an emerging class of small non-coding RNAs implicated in a wide variety of cellular processes. Research in this field is accelerating, and the growing number of miRNAs...
RDML: structured language and reporting guidelines for real-time quantitative PCR data
Lefever, Steve, Hellemans, Jan, Pattyn, Filip, Przybylski, Daniel R., Taylor, Chris, Geurts, René, ...
The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exchange of qPCR data and related information between...
RTPrimerDB: the portal for real-time PCR primers and probes
Lefever, Steve, Vandesompele, Jo, Speleman, Frank, Pattyn, Filip
RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked...
Van Roy, Nadine, De Preter, Katleen, Hoebeeck, Jasmien, Van Maerken, Tom, Pattyn, Filip, Mestdagh, Pieter, ...
Neuroblastoma is one of the most common solid tumors of childhood, arising from immature sympathetic nervous system cells. The clinical course of patients with neuroblastoma is highly variable,...
A novel and universal method for microRNA RT-qPCR data normalization
Mestdagh, Pieter, Van Vlierberghe, Pieter, De Weer, An, Muth, Daniel, Westermann, Frank, Speleman, Frank, ...
The mean expression value: a new method for accurate and reliable normalization of microRNA expression data from RT-qPCR experiments.
Westermann, Frank, Muth, Daniel, Benner, Axel, Bauer, Tobias, Henrich, Kai-Oliver, Oberthuer, André, ...
Differences in MYCN/c-MYC target gene expression are associated with distinct neuroblastoma subtypes and clinical outcome.
Bray, Isabella, Bryan, Kenneth, Prenter, Suzanne, Buckley, Patrick G., Foley, Niamh H., Murphy, Derek M., ...
MiRNAs regulate gene expression at a post-transcriptional level and their dysregulation can play major roles in the pathogenesis of many different forms of cancer, including neuroblastoma, an often...