Job Dekker

Long-range chromosomal interactions and gene regulation (2008)

Miele, Angela, Dekker, Job

Over the last few years important new insights into the process of long-range gene regulation have been obtained. Gene regulatory elements are found to engage in direct physical interactions with...

Gene regulation in the third dimension (2008)

Dekker, Job

Analysis of the spatial organization of chromosomes reveals complex three-dimensional networks of chromosomal interactions. These interactions affect gene expression at multiple levels, including...

A mechanism for Ikaros regulation of human globin gene switching (2008)

Keys, Janelle R., Tallack, Michael R., Zhan, Ye, Papathanasiou, Peter, Doodnow, Christopher C., Gaensler, Karin M., ...

The human globin locus consists of an upstream LCR and functional genes arranged sequentially in teh order of their expression during development: 5'-HBE1, HBG2, HBG1, HBB-3'. Haemoglobin switching...

GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3p (2007)

Dekker, Job

BACKGROUND: Base-composition varies throughout the genome and is related to organization of chromosomes in distinct domains (isochores). Isochore domains differ in gene expression levels, replication...

GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3p (2007)

Dekker, Job

Abstract Background Base-composition varies throughout the genome and is related to organization of chromosomes in distinct domains (isochores). Isochore domains differ in gene expression levels,...

Chromosome Conformation Capture Carbon Copy (5C): a massively parallel solution for mapping interactions between genomic elements (2006)

Dostie, Josee, Richmond, Joann M., Arnaout, Ramy A., Selzer, Rebecca R., Lee, William L., Honan, Tracey A., ...

Physical interactions between genetic elements located throughout the genome play important roles in gene regulation and can be identified with the Chromosome Conformation Capture (3C) methodology....

The active FMR1 promoter is associated with a large domain of altered chromatin conformation with embedded local histone modifications (2006)

Gheldof, Nele, Tabuchi, Tomoko M., Dekker, Job

We have analyzed the effects of gene activation on chromatin conformation throughout an approximately 170-kb region comprising the human fragile X locus, which includes a single expressed gene, FMR1...

Chromosome Conformation Capture Carbon Copy (5C): A massively parallel solution for mapping interactions between genomic elements (2006)

Dostie, Josée, Richmond, Todd A., Arnaout, Ramy A., Selzer, Rebecca R., Lee, William L., Honan, Tracey A., ...

Physical interactions between genetic elements located throughout the genome play important roles in gene regulation and can be identified with the Chromosome Conformation Capture (3C) methodology....

Chromosome Conformation Capture Carbon Copy (5C): A massively parallel solution for mapping interactions between genomic elements (2006)

Dostie, Josée, Richmond, Todd A., Arnaout, Ramy A., Selzer, Rebecca R., Lee, William L., Honan, Tracey A., ...

Physical interactions between genetic elements located throughout the genome play important roles in gene regulation and can be identified with the Chromosome Conformation Capture (3C) methodology....

Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p

Cha, Rita S., Weiner, Beth M., Keeney, Scott, Dekker, Job, Kleckner, N.

Spo11p is a key mediator of interhomolog interactions during meiosis. Deletion of the SPO11 gene decreases the length of S phase by ∼25%. Rec8p is a key coordinator of meiotic interhomolog and...

A mechanical basis for chromosome function

Kleckner, Nancy, Zickler, Denise, Jones, Gareth H., Dekker, Job, Padmore, Ruth, Henle, Jim, ...

We propose that chromosome function is governed by internal mechanical forces generated by programmed tendencies for expansion of the DNA/chromatin fiber against constraining features.

Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p

Cha, Rita S., Weiner, Beth M., Keeney, Scott, Dekker, Job, Kleckner, N.

Spo11p is a key mediator of interhomolog interactions during meiosis. Deletion of the SPO11 gene decreases the length of S phase by ∼25%. Rec8p is a key coordinator of meiotic interhomolog and...

A mechanical basis for chromosome function

Kleckner, Nancy, Zickler, Denise, Jones, Gareth H., Dekker, Job, Padmore, Ruth, Henle, Jim, ...

We propose that chromosome function is governed by internal mechanical forces generated by programmed tendencies for expansion of the DNA/chromatin fiber against constraining features.

The active FMR1 promoter is associated with a large domain of altered chromatin conformation with embedded local histone modifications

Gheldof, Nele, Tabuchi, Tomoko M., Dekker, Job

We have analyzed the effects of gene activation on chromatin conformation throughout an ≈170-kb region comprising the human fragile X locus, which includes a single expressed gene, FMR1 (fragile X...

Chromosome Conformation Capture Carbon Copy (5C): A massively parallel solution for mapping interactions between genomic elements

Dostie, Josée, Richmond, Todd A., Arnaout, Ramy A., Selzer, Rebecca R., Lee, William L., Honan, Tracey A., ...

Physical interactions between genetic elements located throughout the genome play important roles in gene regulation and can be identified with the Chromosome Conformation Capture (3C) methodology....

GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3p

Dekker, Job

GC-rich and AT-rich chromatin domains display distinct chromatin conformations and are marked by distinct patterns of histone modifications, and the histone deacetylase Rpd3p is an attenuator of...

Yeast Silent Mating Type Loci Form Heterochromatic Clusters through Silencer Protein-Dependent Long-Range Interactions

Miele, Adriana, Bystricky, Kerstin, Dekker, Job

The organization of eukaryotic genomes is characterized by the presence of distinct euchromatic and heterochromatic sub-nuclear compartments. In Saccharomyces cerevisiae heterochromatic loci,...

Disease-Causing 7.4 kb Cis-Regulatory Deletion Disrupting Conserved Non-Coding Sequences and Their Interaction with the FOXL2 Promotor: Implications for Mutation Screening

D'haene, Barbara, Attanasio, Catia, Beysen, Diane, Dostie, Josée, Lemire, Edmond, Bouchard, Philippe, ...

To date, the contribution of disrupted potentially cis-regulatory conserved non-coding sequences (CNCs) to human disease is most likely underestimated, as no systematic screens for putative...

Mechanisms that regulate localization of a DNA double-strand break to the nuclear periphery

Oza, Pranav, Jaspersen, Sue L., Miele, Adriana, Dekker, Job, Peterson, Craig L.

DNA double-strand breaks (DSBs) are among the most deleterious forms of DNA lesions in cells. Here we induced site-specific DSBs in yeast cells and monitored chromatin dynamics surrounding the DSB...