Sinnaeve, Davy, Michaux, Catherine, Van Hemel, Johan, Vandenkerckhove, Jan, Peys, Eric, Borremans, Frans, ...
Two new cyclic lipodepsipeptides named pseudodesmin A and B have been isolated from Pseudomonas bacteria collected from the mucus layer in the skin of the black belly salamander. Both compounds show...
Delaude, Lionel, Demonceau, Albert, Wouters, Johan
Five imidazol(in)ium-2-dithiocarboxylates bearing cyclohexyl, mesityl, or 2,6-diisopropylphenyl substituents on their nitrogen atoms were prepared from the corresponding N-heterocyclic carbenes...
Delaude, Lionel, Sauvage, Xavier, Demonceau, Albert, Wouters, Johan
The ability of five imidazol(in)ium-2-carboxylates and dithiocarboxylates bearing cyclohexyl, mesityl, or 2,6-diisopropylphenyl substituents on their nitrogen atoms to act as NHC precursors for in...
Protecting role of cosolvents in protein denaturation by SDS: a structural study (2008)
Michaux, Catherine, Pouyez, Jenny, Wouters, Johan, Privé, Gilbert G
Abstract Background Recently, we reported a unique approach to preserve the activity of some proteins in the presence of the denaturing agent, Sodium Dodecyl Sulfate (SDS). This was made possible by...
Species distribution and coordination of uranyl chloro complexes in acetonitrile (2008)
Servaes, Kelly, Wouters, Johan, ...
The complex formation of the uranyl ion, UO22+, with chloride ions in acetonitrile has been investigated by factor analysis of UV-vis absorption and U L-3 edge EXAFS (extended X-ray absorption fine...
When will synthetic speech sound human: Role of rules and data (2008)
Jan Van Santen, Michael Macon, Andrew Cronk, Paul Hosom, Er Kain, Johan Wouters
Text-to-speech synthesis research has moved away from building general purpose systems based on an understanding of human language and speech production towards building systems based on statistical...
Graulich, Amaury, Lamy, Cédric, Alleva, Livia, Dilly, Sébastien, Chavatte, Philippe, Wouters, Johan, ...
So far, small conductance Ca2+-activated K+ channel (SK) blockers mostly consist of quaternary ammonium derivatives or peptides. Due to their physicochemical properties, these blockers are not...
Calliauw, Gijs, Vila Ayala, José, Gibon, Véronique, Wouters, Johan, De Greyt, Wim, Foubert, Imogen, ...
BMC Structural Biology BioMed Central (2008)
Catherine Michaux, Jenny Pouyez, Johan Wouters, Johan Wouters, Gilbert G Privé
Research article Protecting role of cosolvents in protein denaturation by SDS: a structural study
Unit fusion for concatenative speech synthesis (2007)
Johan Wouters, Michael W. Macon
An important problem in concatenative synthesis is the occurence of spectral discontinuities or “concatenation mismatch ” between sonorant speech units. In this paper, we present an approach to...
Johan Wouters, A Catherine, A Virginie Durbecq, Martine Roovers, B Victor, Stalon A, ...
Preliminary X-ray crystallographic analysis of tRNA pseudouridine 55 synthase from the thermophilic eubacterium Thermotoga maritima Thermotoga maritima TruB, an enzyme responsible for the formation...
Mergeay, Max, Tricot , Catherine, Van Aelst, Sébastien, Wattiez , Ruddy, Stalon, Victor, Wouters , Johan
CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH...
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase (2004)
Roovers, Martine, Wouters, Johan, Bujnicki, Janusz M., Tricot, Catherine, Stalon, Victor, Grosjean, Henri, ...
The modified nucleoside 1‐methyladenosine (m1A) is found in the T‐loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T‐loop...
Wavpred: A Wavelet-Based Algorithm for the Prediction of Transmembrane Proteins (2003)
Baudoux, Guy, Fischer, Patrick, Wouters, Johan
Mathematical filtering using wavelet transform was applied to analyse hydropathy signals of membrane proteins. The accuracy of our localization of transmembrane approaches that of well-established...
Wavpred: A Wavelet-Based Algorithm For The Prediction Of Transmembrane Proteins (2003)
Patrick Fischer, Guy Baudoux, Johan Wouters
Mathematical ltering using wavelet transform was applied to analyse hydropathy signals of membrane proteins. The accuracy of our localization of transmembrane approaches that of well-established...
Prosodic factors for predicting local pitch shape (2002)
Esther Klabbers, Jan Van Santen, Johan Wouters
In this paper, we investigate the predictive power of different prosodic factorization schemes with respect to pitch movement. We will use this to propose an extension of a standard diphone database...
Prosodic factors for predicting local pitch shape (2002)
Esther Klabbers, Jan Van Santen, Johan Wouters
In this paper, we investigate the predictive power of different prosodic factorization schemes with respect to pitch movement. We will use this to propose an extension of a standard diphone database...
Prosodic factors for predicting local pitch shape (2002)
Esther Klabbers, Jan Van Santen, Johan Wouters
In this paper, we investigate the predictive power of different prosodic factorization schemes with respect to pitch movement. We will use this to propose an extension of a standard diphone database...
Control of Spectral Dynamics in Concatenative Speech Synthesis (2001)
Johan Wouters, Student Member, Michael W. Macon
Abstract | Current speech synthesis methods based on the concatenation of waveform units can produce highly intelligible speech capturing the identity of a particular speaker. However, the quality of...
Spectral modification for concatenative speech synthesis (2000)
Johan Wouters, Michael W. Macon
Concatenative synthesis can produce high-quality speech but is limited to the allophonic variations and voice types that were captured in the database. It would be desirable to modify speech units to...
Spectral modification for concatenative speech synthesis (2000)
Johan Wouters, Michael W. Macon
Concatenative synthesis can produce high-quality speech but is limited to the allophonic variations and voice types that were captured in the database. It would be desirable to modify speech units to...
Ron Cole, Dominic W. Massaro, Jacques De Villiers, Brian Rundle, Khaldoun Shobaki, Johan Wouters, ...
This article describes our experiences with an animated conversational agent being used in daily classroom activities with profoundly deaf children at the Tucker Maxon Oral School in Portland Oregon....
Ron Cole, Dominic W. Massaro, Jacques De Villiers, Brian Rundle, Khaldoun Shobaki, Johan Wouters, ...
This article describes our experiences with an animated conversational agent being used in daily classroom activities with profoundly deaf children at the Tucker Maxon Oral School in Portland Oregon....
New tools for interactive speech and language training: Using animated (1999)
Conversational Agents In, Ron Cole, Dominic W. Massaro, Jacques De Villiers, Brian Rundle, Khaldoun Shobaki, ...
This article describes our experiences with an animated conversational agent being used in daily classroom activities with profoundly deaf children at the Tucker Maxon Oral School in Portland Oregon....
A perceptual evaluation of distance measures for concatenative speech synthesis (1998)
Johan Wouters, Michael W. Macon
In concatenative synthesis, new utterances are created by concatenating segments (units) of recorded speech. When the segments are extracted from a large speech corpus, a key issue is to select...
Generalization And Discrimination In Tree-Structured Unit Selection (1998)
Michael W. Macon, Andrew E. Cronk, Johan Wouters
Concatenative "selection-based" synthesis from large databases has emerged as a viable framework for TTS waveform generation. Unit selection algorithms attempt to predict the...
A Perceptual Evaluation Of Distance Measures For Concatenative Speech Synthesis (1998)
Johan Wouters, Michael W. Macon
In concatenative synthesis, new utterances are created by concatenating segments (units) of recorded speech. When the segments are extracted from a large speech corpus, a key issue is to select...
Intelligent Animated Agents for Interactive Language Training (1998)
Ron Cole Tim, Tim Carmell, Pam Connors, Mike Macon, Johan Wouters, ...
This report describes a three-year project, now eight months old, to develop interactive learning tools for language training with profoundly deaf children. The tools combine four key technologies:...
Universal Speech Tools: The Cslu Toolkit (1998)
Stephen Sutton, Ronald Cole, Jacques De Villiers, Johan Schalkwyk, Pieter Vermeulen, Mike Macon, ...
A set of freely available, universal speech tools is needed to accelerate progress in the speech technology. The CSLU Toolkit represents an effort to make the core technology and fundamental...
Michael W. Macon Andrew E. Cronk Johan Wouters (1998)
Center For Spoken, Michael W. Macon, Andrew E. Cronk, Johan Wouters
Concatenative "selection-based" synthesis from large databases has emerged as a viable framework for TTS waveform generation. Unit selection algorithms attempt to predict the...
Intelligent Animated Agents for Interactive Language Training (1998)
Ron Cole, Tim Carmell, Pam Connors, Mike Macon, Johan Wouters, ...
This report describes a three-year project, now eight months old, to develop interactive learning tools for language training with profoundly deaf children. The tools combine four key technologies:...
A Perceptual Evaluation Of Distance Measures For Concatenative Speech Synthesis (1998)
Johan Wouters, Michael W. Macon
In concatenative synthesis, new utterances are created by concatenating segments (units) of recorded speech. When the segments are extracted from a large speech corpus, a key issue is to select...
Universal speech tools: The CSLU toolkit (1998)
Stephen Sutton, Ronald Cole, Jacques De Villiers, Johan Schalkwyk, Pieter Vermeulen, Mike Macon, ...
A set of freely available, universal speech tools is needed to accelerate progress in the speech technology. The CSLU Toolkit represents an effort to make the core technology and fundamental...
Intelligent Animated Agents for Interactive Language Training (1998)
Ron Cole Tim, Tim Carmell, Pam Connors, Mike Macon, Johan Wouters, ...
This report describes a three-year project, now eight months old, to develop interactive learning tools for language training with profoundly deaf children. The tools combine four key technologies:...
Authoring Tools For Speech Synthesis Using The Sable Markup Standard (1996)
Johan Wouters, Brian Rundle, Michael W. Macon
In text-to-speech #TTS# synthesis, input text is automatically analyzed. This involves prediction of pronunciation, intonation, and timing at segmental and phrase level. In the design of dialog...
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase
Roovers, Martine, Wouters, Johan, Bujnicki, Janusz M., Tricot, Catherine, Stalon, Victor, Grosjean, Henri, ...
The modified nucleoside 1-methyladenosine (m1A) is found in the T-loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T-loop of eukaryotic...
Wu, Zheng, Wouters, Johan, Poulter*, C. Dale
Isopentenyl diphosphate isomerase (IDI) catalyzes the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). This is an essential step in the mevalonate entry into...
De Ruyck, Jérôme, Rothman, Steven C., Poulter, C. Dale, Wouters, Johan
Crystal structures of Thermus thermophilus and Bacillus subtilis type 2 IPP isomerases were combined to generate an almost complete model of the FMN-bound structure of the enzyme. In contrast to...
A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase
Roovers, Martine, Wouters, Johan, Bujnicki, Janusz M., Tricot, Catherine, Stalon, Victor, Grosjean, Henri, ...
The modified nucleoside 1-methyladenosine (m1A) is found in the T-loop of many tRNAs from organisms belonging to the three domains of life (Eukaryota, Bacteria, Archaea). In the T-loop of eukaryotic...
Tricot, Catherine, Van Aelst, Sebastien, Wattiez, Ruddy, Mergeay, Max, Stalon, Victor, Wouters, Johan
Overexpression, purification and crystallization of C. metallidurans CopK allowed the collection of a complete data set to 2.2 Å resolution.