John B. Robbins

Protein conjugates of synthetic saccharides elicit higher levels of serum IgG lipopolysaccharide antibodies in mice than do those of the O-specific polysaccharide from Shigella dysenteriae type 1

Pozsgay, Vince, Chu, Chiayung, Pannell, Lewis, Wolfe, Jennifer, Robbins, John B., Schneerson, Rachel

Our development of vaccines to prevent shigellosis is based on the hypothesis that a critical (protective) level of serum IgG to the O-specific polysaccharide (O-SP) domain of Shigella...

A mAb recognizing a surface antigen of Mycobacterium tuberculosis enhances host survival

Teitelbaum, Rachel, Glatman-Freedman, Aharona, Chen, Bing, Robbins, John B., Unanue, Emil, Casadevall, Arturo, ...

Murine mAbs reactive with the surface of Mycobacterium tuberculosis were assayed for their ability to affect the course of infection in mice challenged with virulent organisms. An IgG3 mAb (9d8)...

Serum Immunoglobulin G Antibody Responses to Bordetella pertussis Lipooligosaccharide and B. parapertussis Lipopolysaccharide in Children with Pertussis and Parapertussis

Trollfors, Birger, Lagergård, Teresa, Taranger, John, Bergfors, Elisabet, Schneerson, Rachel, Robbins, John B.

Serum immunoglobulin G (IgG) antibodies against the lipooligosaccharide (LOS) of Bordetella pertussis and the lipopolysaccharide (LPS) of Bordetella parapertussis were measured by enzyme-linked...

Treatment with Succinic Anhydride Improves the Immunogenicity of Shigella flexneri Type 2a O-Specific Polysaccharide–Protein Conjugates in Mice

Pavliakova, Danka, Chu, Chiayung, Bystricky, Slavomir, Tolson, Nathaniel W., Shiloach, Joseph, Kaufman, Jeanne B., ...

Seroepidemiological data and a clinical trial with a Shigella sonnei O-specific polysaccharide (O-SP)–Pseudomonas aeruginosa recombinant exoprotein A (rEPA) conjugate provide evidence that a...

Safety and Immunogenicity of Vi Conjugate Vaccines for Typhoid Fever in Adults, Teenagers, and 2- to 4-Year-Old Children in Vietnam

Kossaczka, Zuzana, Lin, Feng-Ying C., Ho, Vô Anh, Thuy, Nguyen Thi Thanh, Bay, Phan Van, Thanh, Tran Cong, ...

The capsular polysaccharide of Salmonella typhi, Vi, is an essential virulence factor and a protective vaccine for people older than 5 years. The safety and immunogenicity of two investigational Vi...

Syntheses and Immunologic Properties of Escherichia coli O157 O-Specific Polysaccharide and Shiga Toxin 1 B Subunit Conjugates in Mice

Konadu, Edward, Donohue-Rolfe, Arthur, Calderwood, Stephen B., Pozsgay, Vince, Shiloach, Joseph, Robbins, John B., ...

Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers,...

Phase 1 and Phase 2 Studies of Salmonella enterica Serovar Paratyphi A O-Specific Polysaccharide-Tetanus Toxoid Conjugates in Adults, Teenagers, and 2- to 4-Year-Old Children in Vietnam

Konadu, Edward Y., Lin, Feng-Ying C., Hó, Vô Anh, Thuy, Nguyen Thi Thanh, Van Bay, Phan, Thanh, Tran Cong, ...

Salmonella enterica serovar Paratyphi A O-specific polysaccharide (O-SP) was activated with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) and bound to tetanus toxoid (TT) with adipic...

Clostridium difficile Recombinant Toxin A Repeating Units as a Carrier Protein for Conjugate Vaccines: Studies of Pneumococcal Type 14, Escherichia coli K1, and Shigella flexneri Type 2a Polysaccharides in Mice

Pavliakova, Danka, Moncrief, J. Scott, Lyerly, David M., Schiffman, Gerald, Bryla, Dolores A., Robbins, John B., ...

Unlike the native protein, a nontoxic peptide (repeating unit of the native toxin designated rARU) from Clostridium difficile toxin A (CDTA) afforded an antigen that could be bound covalently to the...

Safety and Immunogenicity of Improved Shigella O-Specific Polysaccharide-Protein Conjugate Vaccines in Adults in Israel

Passwell, Justen H., Harlev, Efrat, Ashkenazi, Shai, Chu, Chiayung, Miron, Dan, Ramon, Reut, ...

Data suggest that the O-specific polysaccharide (O-SP) domain of the lipopolysaccharide (LPS) of Shigella species is both an essential virulence factor and a protective antigen and that a critical...

Expression of a Mycobacterium tuberculosis Arabinomannan Antigen In Vitro and In Vivo

Schwebach, J. Reid, Casadevall, Arturo, Schneerson, Rachel, Dai, Zhongdong, Wang, Xiaojuan, Robbins, John B., ...

The outermost layer of Mycobacterium tuberculosis contains two major polysaccharides, arabinomannan (AM) and glucan (GC). We studied the in vitro and in vivo expression of an M. tuberculosis AM...

Vibrio cholerae O139 Conjugate Vaccines: Synthesis and Immunogenicity of V. cholerae O139 Capsular Polysaccharide Conjugates with Recombinant Diphtheria Toxin Mutant in Mice

Kossaczka, Zuzana, Shiloach, Joseph, Johnson, Virginia, Taylor, David N., Finkelstein, Richard A., Robbins, John B., ...

Epidemiologic and experimental data provide evidence that a critical level of serum immunoglobulin G (IgG) antibodies to the surface polysaccharide of Vibrio cholerae O1 (lipopolysaccharide) and of...

Synthesis and Characterization of Lipooligosaccharide-Based Conjugates as Vaccine Candidates for Moraxella (Branhamella) catarrhalis

Gu, Xin-Xing, Chen, Jing, Barenkamp, Stephen J., Robbins, John B., Tsai, Chao-Ming, Lim, David J., ...

Moraxella (Branhamella) catarrhalis is an important cause of otitis media and sinusitis in children and of lower respiratory tract infections in adults. Lipooligosaccharide (LOS) is a major surface...

Phase 1 Evaluation of Vibrio cholerae O1, Serotype Inaba, Polysaccharide-Cholera Toxin Conjugates in Adult Volunteers

Gupta, Rajesh K., Taylor, David N., Bryla, Dolores A., Robbins, John B., Szu, Shousun C.

Conjugate vaccines were prepared by binding hydrazine-treated lipopolysaccharide (DeALPS) from Vibrio cholerae O1, serotype Inaba, to cholera toxin (CT) variants CT-1 and CT-2. Volunteers (n = 75)...

Glucan Is a Component of the Mycobacterium tuberculosis Surface That Is Expressed In Vitro and In Vivo

Schwebach, J. Reid, Glatman-Freedman, Aharona, Gunther-Cummins, Leslie, Dai, Zhongdong, Robbins, John B., Schneerson, Rachel, ...

The outermost layer of Mycobacterium tuberculosis is composed primarily of two polysaccharides, glucan (GC) and arabinomannan. To analyze the surface polysaccharide composition of M. tuberculosis, we...

Specificity and Diversity of Antibodies to Mycobacterium tuberculosis Arabinomannan

Navoa, Josephine Anne D., Laal, Suman, Pirofski, Liise-Anne, McLean, Gary R., Dai, Zhongdong, Robbins, John B., ...

Arabinomannan (AM) is a polysaccharide antigen of the mycobacterial capsule. However, it is uncertain whether AM constitutes an immunologically distinct fraction of Mycobacterium tuberculosis. In...

Poly(γ-d-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: A potential addition to the anthrax vaccine

Schneerson, Rachel, Kubler-Kielb, Joanna, Liu, Teh-Yung, Dai, Zhong-Dong, Leppla, Stephen H., Yergey, Alfred, ...

Both the protective antigen (PA) and the poly(γ-d-glutamic acid) capsule (γdPGA) are essential for the virulence of Bacillus anthracis. A critical level of vaccine-induced IgG anti-PA confers...

Preparation and Characterization of Group A Meningococcal Capsular Polysaccharide Conjugates and Evaluation of Their Immunogenicity in Mice

Jin, Zhigang, Chu, Chiayung, Robbins, John B., Schneerson, Rachel

Epidemic and endemic meningitis caused by group A Neisseria meningitidis remains a problem in sub-Saharan Africa. Although group A meningococcal capsular polysaccharide (GAMP) vaccine confers...

Further Studies on the Immunogenicity of Haemophilus influenzae Type b and Pneumococcal Type 6A Polysaccharide-Protein Conjugates

Chu, Chiayung, Schneerson, Rachel, Robbins, John B., Rastogi, Suresh C.

Conjugates were prepared by carbodiimide-mediated coupling of adipic acid hydrazide derivatives of Haemophilus influenzae type b (Hib), Escherichia coli K100, and pneumococcal 6A (Pn6A)...

Nonabsorbable Rabbit Anti-Salmonella typhimurium Antibody as Detected by the Complement-Mediated Bactericidal Reaction

Herzberg, Mendel, Kenny, Kathryn V., Robbins, John B.

Herzberg, Mendel (University of Florida, Gainesville), Kathryn V. Kenny, and John B. Robbins. Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated...

Differential Complement Resistance Mediates Virulence of Haemophilus influenzae Type b

Sutton, Ann, Schneerson, Rachel, Kendall-Morris, Saundra, Robbins, John B.

Studies were undertaken to gain insight into the virulence of type b in contrast to the other Haemophilus influenzae capsular types. A relationship was found between the comparative virulence of H....

Cross-Immunogenicity of Pneumococcal Group 9 Capsular Polysaccharides in Adult Volunteers

Szu, Shousun C., Lee, Chi-Jen, Parke, J. C., Schiffman, Gerald, Henrichsen, Jørgen, Austrian, Robert, ...

Group 9 organisms (types 9N, 9A, 9L, and 9V) account for about 3 to 4% of pneumococcal disease isolates throughout the world. Types 9N and 9V comprise about 90% of the group 9 disease isolates. Type...

Comparative Immunogenicity of Group 6 Pneumococcal Type 6A(6) and Type 6B(26) Capsular Polysaccharides

Robbins, John B., Lee, Chi-Jen, Rastogi, Suresh C., Schiffman, Gerald, Henrichsen, Jørgen

The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera...

Specific Secondary Biological Properties of Purified Rabbit Immunoglobulin M and Immunoglobulin G Antibodies to Brucella abortus and Bordetella pertussis

Schulkind, Martin L., Herzberg, Mendel, Robbins, John B.

Rabbit immunoglobulin (Ig)M and IgG antibodies to Brucella abortus and Bordetella pertussis were isolated as purified products and their specific secondary biological activities were compared. IgM...

Age-Related Susceptibility to Haemophilus influenzae Type b Disease in Rabbits

Schneerson, Rachel, Robbins, John B.

Anti-type b antibodies were detected in newborns and in most 6- to 8-week-old rabbits, similar to those observed in humans. Culture of the pharynx and rectum of rabbits at varied ages failed to yield...

Enteric Bacteria Cross-Reactive with Neisseria meningitidis Groups A and C and Diplococcus pneumoniae Types I and III

Robbins, John B., Myerowitz, Richard L., Whisnant, John K., Argaman, Meir, Schneerson, Rachel, Handzel, Zeev T., ...

Enteric bacteria of 1,335 individual strains were studied for serological cross-reactions with Neisseria meningitidis groups A and C and Diplococcus pneumoniae types I and III. Enterobacterial...

Induction of Haemophilus influenzae Type b Capsular Antibody in Neonatal Rabbits by Gastrointestinal Colonization with Cross-Reacting Escherichia coli

Myerowitz, Richard L., Handzel, Zeev T., Schneerson, Rachel, Robbins, John B.

In two separate experiments, newborn rabbits were fed a live suspension of either of two Escherichia coli strains which possess a “K” antigen cross-reactive with the capsular polysaccharide of...

Polysaccharides of the Genus Bacillus Cross-Reactive with the Capsular Polysaccharides of Diplococcus pneumoniae Type III, Haemophilus influenzae Type b, and Neisseria meningitidis Group A

Myerowitz, Richard L., Gordon, Ruth E., Robbins, John B.

We studied 174 strains of the genus Bacillus for cross-reacting antigens to the capsular polysaccharides of groups A and C meningococcus, types I and III pneumococcus, and Haemophilus influenzae type...

Antigenic Evidence of Prevalence and Diversity of Mycobacterium tuberculosis Arabinomannan

Glatman-Freedman, Aharona, Casadevall, Arturo, Dai, Zongdong, Jacobs, William R., Li, Anping, Morris, Sheldon L., ...

Arabinomannan (AM) is a polysaccharide of the mycobacterial capsule. The capsular polysaccharides of various microorganisms are diverse, and this diversity is important for classification of...

Effect of Dosage on Immunogenicity of a Vi Conjugate Vaccine Injected Twice into 2- to 5-Year-Old Vietnamese Children†

Canh, Do Gia, Lin, Feng-ying (Kimi), Thiem, Vu Dinh, Trach, Dang Duc, Trong, Nguyen Dinh, Mao, Nguyen Duc, ...

In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 μg of Vi and...

Additional Conjugation Methods and Immunogenicity of Bacillus anthracis Poly-γ-d-Glutamic Acid-Protein Conjugates

Kubler-Kielb, Joanna, Liu, Teh-Yung, Mocca, Christopher, Majadly, Fathy, Robbins, John B., Schneerson, Rachel

The capsule of Bacillus anthracis, composed of poly-γ-d-glutamic acid (γDPGA), is an essential virulence factor of B. anthracis. The capsule inhibits innate host defense through its antiphagocytic...

Protein conjugates of synthetic saccharides elicit higher levels of serum IgG lipopolysaccharide antibodies in mice than do those of the O-specific polysaccharide from Shigella dysenteriae type 1

Pozsgay, Vince, Chu, Chiayung, Pannell, Lewis, Wolfe, Jennifer, Robbins, John B., Schneerson, Rachel

Our development of vaccines to prevent shigellosis is based on the hypothesis that a critical (protective) level of serum IgG to the O-specific polysaccharide (O-SP) domain of Shigella...

A mAb recognizing a surface antigen of Mycobacterium tuberculosis enhances host survival

Teitelbaum, Rachel, Glatman-Freedman, Aharona, Chen, Bing, Robbins, John B., Unanue, Emil, Casadevall, Arturo, ...

Murine mAbs reactive with the surface of Mycobacterium tuberculosis were assayed for their ability to affect the course of infection in mice challenged with virulent organisms. An IgG3 mAb (9d8)...

Serum Immunoglobulin G Antibody Responses to Bordetella pertussis Lipooligosaccharide and B. parapertussis Lipopolysaccharide in Children with Pertussis and Parapertussis

Trollfors, Birger, Lagergård, Teresa, Taranger, John, Bergfors, Elisabet, Schneerson, Rachel, Robbins, John B.

Serum immunoglobulin G (IgG) antibodies against the lipooligosaccharide (LOS) of Bordetella pertussis and the lipopolysaccharide (LPS) of Bordetella parapertussis were measured by enzyme-linked...

Treatment with Succinic Anhydride Improves the Immunogenicity of Shigella flexneri Type 2a O-Specific Polysaccharide–Protein Conjugates in Mice

Pavliakova, Danka, Chu, Chiayung, Bystricky, Slavomir, Tolson, Nathaniel W., Shiloach, Joseph, Kaufman, Jeanne B., ...

Seroepidemiological data and a clinical trial with a Shigella sonnei O-specific polysaccharide (O-SP)–Pseudomonas aeruginosa recombinant exoprotein A (rEPA) conjugate provide evidence that a...

Safety and Immunogenicity of Vi Conjugate Vaccines for Typhoid Fever in Adults, Teenagers, and 2- to 4-Year-Old Children in Vietnam

Kossaczka, Zuzana, Lin, Feng-Ying C., Ho, Vô Anh, Thuy, Nguyen Thi Thanh, Bay, Phan Van, Thanh, Tran Cong, ...

The capsular polysaccharide of Salmonella typhi, Vi, is an essential virulence factor and a protective vaccine for people older than 5 years. The safety and immunogenicity of two investigational Vi...

Syntheses and Immunologic Properties of Escherichia coli O157 O-Specific Polysaccharide and Shiga Toxin 1 B Subunit Conjugates in Mice

Konadu, Edward, Donohue-Rolfe, Arthur, Calderwood, Stephen B., Pozsgay, Vince, Shiloach, Joseph, Robbins, John B., ...

Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers,...

Phase 1 and Phase 2 Studies of Salmonella enterica Serovar Paratyphi A O-Specific Polysaccharide-Tetanus Toxoid Conjugates in Adults, Teenagers, and 2- to 4-Year-Old Children in Vietnam

Konadu, Edward Y., Lin, Feng-Ying C., Hó, Vô Anh, Thuy, Nguyen Thi Thanh, Van Bay, Phan, Thanh, Tran Cong, ...

Salmonella enterica serovar Paratyphi A O-specific polysaccharide (O-SP) was activated with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) and bound to tetanus toxoid (TT) with adipic...

Clostridium difficile Recombinant Toxin A Repeating Units as a Carrier Protein for Conjugate Vaccines: Studies of Pneumococcal Type 14, Escherichia coli K1, and Shigella flexneri Type 2a Polysaccharides in Mice

Pavliakova, Danka, Moncrief, J. Scott, Lyerly, David M., Schiffman, Gerald, Bryla, Dolores A., Robbins, John B., ...

Unlike the native protein, a nontoxic peptide (repeating unit of the native toxin designated rARU) from Clostridium difficile toxin A (CDTA) afforded an antigen that could be bound covalently to the...

Safety and Immunogenicity of Improved Shigella O-Specific Polysaccharide-Protein Conjugate Vaccines in Adults in Israel

Passwell, Justen H., Harlev, Efrat, Ashkenazi, Shai, Chu, Chiayung, Miron, Dan, Ramon, Reut, ...

Data suggest that the O-specific polysaccharide (O-SP) domain of the lipopolysaccharide (LPS) of Shigella species is both an essential virulence factor and a protective antigen and that a critical...

Expression of a Mycobacterium tuberculosis Arabinomannan Antigen In Vitro and In Vivo

Schwebach, J. Reid, Casadevall, Arturo, Schneerson, Rachel, Dai, Zhongdong, Wang, Xiaojuan, Robbins, John B., ...

The outermost layer of Mycobacterium tuberculosis contains two major polysaccharides, arabinomannan (AM) and glucan (GC). We studied the in vitro and in vivo expression of an M. tuberculosis AM...

Vibrio cholerae O139 Conjugate Vaccines: Synthesis and Immunogenicity of V. cholerae O139 Capsular Polysaccharide Conjugates with Recombinant Diphtheria Toxin Mutant in Mice

Kossaczka, Zuzana, Shiloach, Joseph, Johnson, Virginia, Taylor, David N., Finkelstein, Richard A., Robbins, John B., ...

Epidemiologic and experimental data provide evidence that a critical level of serum immunoglobulin G (IgG) antibodies to the surface polysaccharide of Vibrio cholerae O1 (lipopolysaccharide) and of...

Synthesis and Characterization of Lipooligosaccharide-Based Conjugates as Vaccine Candidates for Moraxella (Branhamella) catarrhalis

Gu, Xin-Xing, Chen, Jing, Barenkamp, Stephen J., Robbins, John B., Tsai, Chao-Ming, Lim, David J., ...

Moraxella (Branhamella) catarrhalis is an important cause of otitis media and sinusitis in children and of lower respiratory tract infections in adults. Lipooligosaccharide (LOS) is a major surface...

Phase 1 Evaluation of Vibrio cholerae O1, Serotype Inaba, Polysaccharide-Cholera Toxin Conjugates in Adult Volunteers

Gupta, Rajesh K., Taylor, David N., Bryla, Dolores A., Robbins, John B., Szu, Shousun C.

Conjugate vaccines were prepared by binding hydrazine-treated lipopolysaccharide (DeALPS) from Vibrio cholerae O1, serotype Inaba, to cholera toxin (CT) variants CT-1 and CT-2. Volunteers (n = 75)...

Glucan Is a Component of the Mycobacterium tuberculosis Surface That Is Expressed In Vitro and In Vivo

Schwebach, J. Reid, Glatman-Freedman, Aharona, Gunther-Cummins, Leslie, Dai, Zhongdong, Robbins, John B., Schneerson, Rachel, ...

The outermost layer of Mycobacterium tuberculosis is composed primarily of two polysaccharides, glucan (GC) and arabinomannan. To analyze the surface polysaccharide composition of M. tuberculosis, we...

Specificity and Diversity of Antibodies to Mycobacterium tuberculosis Arabinomannan

Navoa, Josephine Anne D., Laal, Suman, Pirofski, Liise-Anne, McLean, Gary R., Dai, Zhongdong, Robbins, John B., ...

Arabinomannan (AM) is a polysaccharide antigen of the mycobacterial capsule. However, it is uncertain whether AM constitutes an immunologically distinct fraction of Mycobacterium tuberculosis. In...

Poly(γ-d-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: A potential addition to the anthrax vaccine

Schneerson, Rachel, Kubler-Kielb, Joanna, Liu, Teh-Yung, Dai, Zhong-Dong, Leppla, Stephen H., Yergey, Alfred, ...

Both the protective antigen (PA) and the poly(γ-d-glutamic acid) capsule (γdPGA) are essential for the virulence of Bacillus anthracis. A critical level of vaccine-induced IgG anti-PA confers...

Preparation and Characterization of Group A Meningococcal Capsular Polysaccharide Conjugates and Evaluation of Their Immunogenicity in Mice

Jin, Zhigang, Chu, Chiayung, Robbins, John B., Schneerson, Rachel

Epidemic and endemic meningitis caused by group A Neisseria meningitidis remains a problem in sub-Saharan Africa. Although group A meningococcal capsular polysaccharide (GAMP) vaccine confers...

Further Studies on the Immunogenicity of Haemophilus influenzae Type b and Pneumococcal Type 6A Polysaccharide-Protein Conjugates

Chu, Chiayung, Schneerson, Rachel, Robbins, John B., Rastogi, Suresh C.

Conjugates were prepared by carbodiimide-mediated coupling of adipic acid hydrazide derivatives of Haemophilus influenzae type b (Hib), Escherichia coli K100, and pneumococcal 6A (Pn6A)...

Nonabsorbable Rabbit Anti-Salmonella typhimurium Antibody as Detected by the Complement-Mediated Bactericidal Reaction

Herzberg, Mendel, Kenny, Kathryn V., Robbins, John B.

Herzberg, Mendel (University of Florida, Gainesville), Kathryn V. Kenny, and John B. Robbins. Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated...

Differential Complement Resistance Mediates Virulence of Haemophilus influenzae Type b

Sutton, Ann, Schneerson, Rachel, Kendall-Morris, Saundra, Robbins, John B.

Studies were undertaken to gain insight into the virulence of type b in contrast to the other Haemophilus influenzae capsular types. A relationship was found between the comparative virulence of H....

Cross-Immunogenicity of Pneumococcal Group 9 Capsular Polysaccharides in Adult Volunteers

Szu, Shousun C., Lee, Chi-Jen, Parke, J. C., Schiffman, Gerald, Henrichsen, Jørgen, Austrian, Robert, ...

Group 9 organisms (types 9N, 9A, 9L, and 9V) account for about 3 to 4% of pneumococcal disease isolates throughout the world. Types 9N and 9V comprise about 90% of the group 9 disease isolates. Type...

Comparative Immunogenicity of Group 6 Pneumococcal Type 6A(6) and Type 6B(26) Capsular Polysaccharides

Robbins, John B., Lee, Chi-Jen, Rastogi, Suresh C., Schiffman, Gerald, Henrichsen, Jørgen

The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera...

Specific Secondary Biological Properties of Purified Rabbit Immunoglobulin M and Immunoglobulin G Antibodies to Brucella abortus and Bordetella pertussis

Schulkind, Martin L., Herzberg, Mendel, Robbins, John B.

Rabbit immunoglobulin (Ig)M and IgG antibodies to Brucella abortus and Bordetella pertussis were isolated as purified products and their specific secondary biological activities were compared. IgM...

Age-Related Susceptibility to Haemophilus influenzae Type b Disease in Rabbits

Schneerson, Rachel, Robbins, John B.

Anti-type b antibodies were detected in newborns and in most 6- to 8-week-old rabbits, similar to those observed in humans. Culture of the pharynx and rectum of rabbits at varied ages failed to yield...

Enteric Bacteria Cross-Reactive with Neisseria meningitidis Groups A and C and Diplococcus pneumoniae Types I and III

Robbins, John B., Myerowitz, Richard L., Whisnant, John K., Argaman, Meir, Schneerson, Rachel, Handzel, Zeev T., ...

Enteric bacteria of 1,335 individual strains were studied for serological cross-reactions with Neisseria meningitidis groups A and C and Diplococcus pneumoniae types I and III. Enterobacterial...

Induction of Haemophilus influenzae Type b Capsular Antibody in Neonatal Rabbits by Gastrointestinal Colonization with Cross-Reacting Escherichia coli

Myerowitz, Richard L., Handzel, Zeev T., Schneerson, Rachel, Robbins, John B.

In two separate experiments, newborn rabbits were fed a live suspension of either of two Escherichia coli strains which possess a “K” antigen cross-reactive with the capsular polysaccharide of...

Polysaccharides of the Genus Bacillus Cross-Reactive with the Capsular Polysaccharides of Diplococcus pneumoniae Type III, Haemophilus influenzae Type b, and Neisseria meningitidis Group A

Myerowitz, Richard L., Gordon, Ruth E., Robbins, John B.

We studied 174 strains of the genus Bacillus for cross-reacting antigens to the capsular polysaccharides of groups A and C meningococcus, types I and III pneumococcus, and Haemophilus influenzae type...

Antigenic Evidence of Prevalence and Diversity of Mycobacterium tuberculosis Arabinomannan

Glatman-Freedman, Aharona, Casadevall, Arturo, Dai, Zongdong, Jacobs, William R., Li, Anping, Morris, Sheldon L., ...

Arabinomannan (AM) is a polysaccharide of the mycobacterial capsule. The capsular polysaccharides of various microorganisms are diverse, and this diversity is important for classification of...

Effect of Dosage on Immunogenicity of a Vi Conjugate Vaccine Injected Twice into 2- to 5-Year-Old Vietnamese Children†

Canh, Do Gia, Lin, Feng-ying (Kimi), Thiem, Vu Dinh, Trach, Dang Duc, Trong, Nguyen Dinh, Mao, Nguyen Duc, ...

In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 μg of Vi and...

Additional Conjugation Methods and Immunogenicity of Bacillus anthracis Poly-γ-d-Glutamic Acid-Protein Conjugates

Kubler-Kielb, Joanna, Liu, Teh-Yung, Mocca, Christopher, Majadly, Fathy, Robbins, John B., Schneerson, Rachel

The capsule of Bacillus anthracis, composed of poly-γ-d-glutamic acid (γDPGA), is an essential virulence factor of B. anthracis. The capsule inhibits innate host defense through its antiphagocytic...

Long-lasting and transmission-blocking activity of antibodies to Plasmodium falciparum elicited in mice by protein conjugates of Pfs25

Kubler-Kielb, Joanna, Majadly, Fathy, Wu, Yimin, Narum, David L., Guo, Chunyan, Miller, Louis H., ...

Malaria is a leading cause of morbidity and mortality, estimated to cause >1 million childhood deaths annually. Plasmodium falciparum causes the most severe form of the disease. There is as yet no...

Effect of the nonreducing end of Shigella dysenteriae type 1 O-specific oligosaccharides on their immunogenicity as conjugates in mice

Pozsgay, Vince, Kubler-Kielb, Joanna, Schneerson, Rachel, Robbins, John B.

Endemic and epidemic shigellosis, an acute invasive disease of the lower intestines, afflicts millions of people worldwide with an estimated one million fatalities per annum at a low infectious dose....

THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES

Robbins, John B., Kenny, Kathryn, Suter, Emanuel

Rabbit γM- and γG-anti-S. typhimurium antibodies were isolated by combined immune specific and physical methods and some of their properties in immunological systems were measured. γM-Antibody was...

THE DEVELOPMENT OF THE IMMUNE RESPONSE : STUDIES ON THE AGGLUTININ RESPONSE TO SALMONELLA FLAGELLAR ANTIGENS IN THE NEWBORN RABBIT

Bellanti, Joseph A., Eitzman, Donald V., Robbins, John B., Smith, Richard T.

By intensive stimulation with large amounts of Salmonella flagellar antigen, newborn rabbits were induced to form high titer flagellar agglutinins usually by the 7th to 10th day of life....

Saccharides cross-reactive with Bacillus anthracis spore glycoprotein as an anthrax vaccine component

Kubler-Kielb, Joanna, Vinogradov, Evgeny, Hu, Haijing, Leppla, Stephen H., Robbins, John B., Schneerson, Rachel

Bacillus anthracis is a spore-forming bacterium that causes anthrax in humans and in other mammals. The glycoprotein BclA (Bacillus collagen-like protein of anthracis) is a major constituent of the...

Meningococcal meningitis in sub-Saharan Africa: the case for mass and routine vaccination with available polysaccharide vaccines.

Robbins, John B., Schneerson, Rachel, Gotschlich, Emil C., Mohammed, Idris, Nasidi, Abdulsalami, Chippaux, Jean-Philippe, ...

Endemic and epidemic group A meningococcal meningitis remains a major cause of morbidity and mortality in sub-Saharan Africa, despite the availability of the safe and inexpensive group A...

Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugates

Robbins, John B., Kubler-Kielb, Joanna, Vinogradov, Evguenii, Mocca, Christopher, Pozsgay, Vince, Shiloach, Joseph, ...

Shigellosis, an enteric disease, is on the World Health Organization's priority prevention list. In one study, the Shigella sonnei O-specific polysaccharide (O-SP)-protein conjugate showed 72%...