Ursula W. Goodenough, John E. Heuser
ABSTRACT The substructure of the components of the axoneme interior--the inner dynein arms, the radial spokes, and the central pair/projection complex--was analyzed for Chlamy-domonas, Tetrahymena,...
A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments (2008)
Sheridan, Sean D., Yu, Xiong, Roth, Robyn, Heuser, John E., Sehorn, Michael G., Sung, Patrick, ...
The eukaryotic RecA homologs Rad51 and Dmc1 are essential for strand exchange between homologous chromosomes during meiosis. All members of the RecA family of recombinases polymerize on DNA to form...
Edeling, Melissa A., Mishra, Sanjay K., Keyel, Peter A., Steinhauser, Amie L., Collins, Brett M., Roth, Robyn, ...
Clathrin-associated sorting proteins (CLASPs) expand the repertoire of endocytic cargo sorted into clathrin-coated vesicles beyond the transmembrane proteins that bind physically to the AP-2 adaptor....
Edeling, Melissa A., Mishra, Sanjay K., Keyel, Peter A., Steinhauser, Amie L., Collins, Brett M., Roth, Robyn, ...
Clathrin-associated sorting proteins (CLASPs) expand the repertoire of endocytic cargo sorted into clathrin-coated vesicles beyond the transmembrane proteins that bind physically to the AP-2 adaptor....
Abstract. Two seemingly unrelated experimental treat-ments inhibit receptor mediated endocytosis: (a) deple-
Substructure of the outer dynein arm (1982)
Ursula W. Goodenough, John E. Heuser
ABSTRACT The substructure of the outer dynein arm has been analyzed in quick-frozen deep-etch replicas of Tetrahymena and Chlamydornonas axonemes. Each arm is found to be composed of five...
Surface structure of the COPII-coated vesicle
Matsuoka, Ken, Schekman, Randy, Orci, Lelio, Heuser, John E.
The spatial arrangement of COPII coat protein subunits was analyzed by crosslinking to an artificial membrane surface and by electron microscopy of coat proteins and coated vesicle surfaces. The...
Cell Vacuolation Caused by Vibrio cholerae Hemolysin
Figueroa-Arredondo, Paula, Heuser, John E., Akopyants, Natalia S., Morisaki, J. Hiroshi, Giono-Cerezo, Silvia, Enríquez-Rincón, Fernando, ...
Non-O1 strains of Vibrio cholerae implicated in gastroenteritis and diarrhea generally lack virulence determinants such as cholera toxin that are characteristic of epidemic strains; the factors that...
Cortactin Is a Component of Clathrin-Coated Pits and Participates in Receptor-Mediated Endocytosis
Cao, Hong, Orth, James D., Chen, Jing, Weller, Shaun G., Heuser, John E., McNiven, Mark A.
The actin cytoskeleton is believed to contribute to the formation of clathrin-coated pits, although the specific components that connect actin filaments with the endocytic machinery are unclear....
Sexton, Jessica A., Pinkner, Jerome S., Roth, Robyn, Heuser, John E., Hultgren, Scott J., Vogel, Joseph P.
The ability of Legionella pneumophila to grow and cause disease in the host is completely dependent on a type IV secretion system known as the Dot/Icm complex. This membrane-spanning apparatus...
Zhang, Claire X., Carreno, Sebastien, Barroso, Consuelo, Heuser, John E., Drubin, David G.
Actin filaments transiently associate with the endocytic machinery during clathrin-coated vesicle formation. Although several proteins that might mediate or regulate this association have been...
Chlamydomonas agglutinin is a hydroxyproline-rich glycoprotein
Cooper, James B., Adair, W. Steven, Mecham, Robert P., Heuser, John E.
The mt+ sexual agglutinin from Chlamydomonas reinhardi is shown to contain ≈12% hydroxyproline, and two inhibitors of hydroxyproline formation, α,α′-dipyridyl and 3,4-dehydroproline, are shown...
TorsinA in the nuclear envelope
Naismith, Teresa V., Heuser, John E., Breakefield, Xandra O., Hanson, Phyllis I.
Early-onset torsion dystonia, a CNS-based movement disorder, is usually associated with a single amino acid deletion (ΔE302/303) in the protein torsinA. TorsinA is an AAA+ ATPase in the endoplasmic...
Keyel, Peter A., Mishra, Sanjay K., Roth, Robyn, Heuser, John E., Watkins, Simon C., Traub, Linton M.
Sorting of transmembrane cargo into clathrin-coated vesicles requires endocytic adaptors, yet RNA interference (RNAi)-mediated gene silencing of the AP-2 adaptor complex only disrupts internalization...
Surface structure of the COPII-coated vesicle
Matsuoka, Ken, Schekman, Randy, Orci, Lelio, Heuser, John E.
The spatial arrangement of COPII coat protein subunits was analyzed by crosslinking to an artificial membrane surface and by electron microscopy of coat proteins and coated vesicle surfaces. The...
Cell Vacuolation Caused by Vibrio cholerae Hemolysin
Figueroa-Arredondo, Paula, Heuser, John E., Akopyants, Natalia S., Morisaki, J. Hiroshi, Giono-Cerezo, Silvia, Enríquez-Rincón, Fernando, ...
Non-O1 strains of Vibrio cholerae implicated in gastroenteritis and diarrhea generally lack virulence determinants such as cholera toxin that are characteristic of epidemic strains; the factors that...
Cortactin Is a Component of Clathrin-Coated Pits and Participates in Receptor-Mediated Endocytosis
Cao, Hong, Orth, James D., Chen, Jing, Weller, Shaun G., Heuser, John E., McNiven, Mark A.
The actin cytoskeleton is believed to contribute to the formation of clathrin-coated pits, although the specific components that connect actin filaments with the endocytic machinery are unclear....
Sexton, Jessica A., Pinkner, Jerome S., Roth, Robyn, Heuser, John E., Hultgren, Scott J., Vogel, Joseph P.
The ability of Legionella pneumophila to grow and cause disease in the host is completely dependent on a type IV secretion system known as the Dot/Icm complex. This membrane-spanning apparatus...
Zhang, Claire X., Carreno, Sebastien, Barroso, Consuelo, Heuser, John E., Drubin, David G.
Actin filaments transiently associate with the endocytic machinery during clathrin-coated vesicle formation. Although several proteins that might mediate or regulate this association have been...
Chlamydomonas agglutinin is a hydroxyproline-rich glycoprotein
Cooper, James B., Adair, W. Steven, Mecham, Robert P., Heuser, John E.
The mt+ sexual agglutinin from Chlamydomonas reinhardi is shown to contain ≈12% hydroxyproline, and two inhibitors of hydroxyproline formation, α,α′-dipyridyl and 3,4-dehydroproline, are shown...
TorsinA in the nuclear envelope
Naismith, Teresa V., Heuser, John E., Breakefield, Xandra O., Hanson, Phyllis I.
Early-onset torsion dystonia, a CNS-based movement disorder, is usually associated with a single amino acid deletion (ΔE302/303) in the protein torsinA. TorsinA is an AAA+ ATPase in the endoplasmic...
Keyel, Peter A., Mishra, Sanjay K., Roth, Robyn, Heuser, John E., Watkins, Simon C., Traub, Linton M.
Sorting of transmembrane cargo into clathrin-coated vesicles requires endocytic adaptors, yet RNA interference (RNAi)-mediated gene silencing of the AP-2 adaptor complex only disrupts internalization...
Heuser, John E., Doggenweiler, Carlos F.
In view of reports that the nerve fibers of the sea prawn conduct impulses more rapidly than other invertebrate nerves and look like myelinated vertebrate nerves in the light microscope, prawn nerve...
Doggenweiler, C. F., Heuser, John E.
The sheaths from freshly teased nerve fibers of the prawn exhibit a positive radial birefringence, consistent with their EM appearance as highly organized laminated structures composed of numerous...
AUTORADIOGRAPHIC LOCALIZATION OF NEW RNA SYNTHESIS IN HYPERTROPHYING SKELETAL MUSCLE
Jablecki, Charles K., Heuser, John E., Kaufman, Seymour
Work-induced growth of rat soleus muscle is accompanied by an early increase in new RNA synthesis. To determine the cell type(s) responsible for the increased RNA synthesis, we compared light...
Cover, Timothy L., Hanson, Phyllis I., Heuser, John E.
In this study, we describe the ultrastructural changes associated with acid activation of Helicobacter pylori vacuolating cytotoxin (VacA). Purified VacA molecules imaged by deep-etch electron...
Ungar, Daniel, Oka, Toshihiko, Brittle, Elizabeth E., Vasile, Eliza, Lupashin, Vladimir V., Chatterton, Jon E., ...
Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Three complexes that have previously been...
Eckley, D. Mark, Gill, Steven R., Melkonian, Karin A., Bingham, James B., Goodson, Holly V., Heuser, John E., ...
The multisubunit protein, dynactin, is a critical component of the cytoplasmic dynein motor machinery. Dynactin contains two distinct structural domains: a projecting sidearm that interacts with...
Assembly of Weibel–Palade body-like tubules from N-terminal domains of von Willebrand factor
Huang, Ren-Huai, Wang, Ying, Roth, Robyn, Yu, Xiong, Purvis, Angie R., Heuser, John E., ...
Endothelial cells assemble von Willebrand factor (VWF) multimers into ordered tubules within storage organelles called Weibel–Palade bodies, and tubular packing is necessary for the secretion of...
A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments
Sheridan, Sean D., Yu, Xiong, Roth, Robyn, Heuser, John E., Sehorn, Michael G., Sung, Patrick, ...
The eukaryotic RecA homologs Rad51 and Dmc1 are essential for strand exchange between homologous chromosomes during meiosis. All members of the RecA family of recombinases polymerize on DNA to form...
Plasma membrane deformation by circular arrays of ESCRT-III protein filaments
Hanson, Phyllis I., Roth, Robyn, Lin, Yuan, Heuser, John E.
Endosomal sorting complex required for transport III (ESCRT-III) proteins function in multivesicular body biogenesis and viral budding. They are recruited from the cytoplasm to the membrane, where...
Kim, Yumi, Heuser, John E., Waterman, Clare M., Cleveland, Don W.
The mitotic kinesin centromere protein E (CENP-E) is an essential kinetochore component that directly contributes to the capture and stabilization of spindle microtubules by kinetochores. Although...
The AP-2 Adaptor β2 Appendage Scaffolds Alternate Cargo Endocytosis
Keyel, Peter A., Thieman, James R., Roth, Robyn, Erkan, Elif, Everett, Eric T., Watkins, Simon C., ...
The independently folded appendages of the large α and β2 subunits of the endocytic adaptor protein (AP)-2 complex coordinate proper assembly and operation of endocytic components during...
Martens, Eric C., Roth, Robyn, Heuser, John E., Gordon, Jeffrey I.
Bacteria in the distal human gut have evolved diverse abilities to metabolize complex glycans, including the capacity to degrade these compounds as nutrients and to assemble their component sugars...
Soman, Neelesh R., Baldwin, Steven L., Hu, Grace, Marsh, Jon N., Lanza, Gregory M., Heuser, John E., ...
The in vivo application of cytolytic peptides for cancer therapeutics is hampered by toxicity, nonspecificity, and degradation. We previously developed a specific strategy to synthesize a nanoscale...