John G. Gribben

Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells

Görgün, Güllü, Holderried, Tobias A.W., Zahrieh, David, Neuberg, Donna, Gribben, John G.

To examine the impact of tumors on the immune system, we compared global gene expression profiles of peripheral blood T cells from previously untreated patients with B cell chronic lymphocytic...

Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells

Görgün, Güllü, Holderried, Tobias A.W., Zahrieh, David, Neuberg, Donna, Gribben, John G.

To examine the impact of tumors on the immune system, we compared global gene expression profiles of peripheral blood T cells from previously untreated patients with B cell chronic lymphocytic...

Autologous and allogeneic stem cell transplantations for poor-risk chronic lymphocytic leukemia

Gribben, John G., Zahrieh, David, Stephans, Katherine, Bartlett-Pandite, Lini, Alyea, Edwin P., Fisher, David C., ...

We report here on the long-term follow-up on 162 patients with high-risk chronic lymphocytic leukemia (CLL) who have undergone hematopoietic stem cell transplantation (SCT) at a single center from...

Cytotoxic T cells generated against heteroclitic peptides kill primary tumor cells independent of the binding affinity of the native tumor antigen peptide

Zirlik, Katja Mauerer, Zahrieh, David, Neuberg, Donna, Gribben, John G.

Heteroclitic peptide modifications increase immunogenicity, allowing generation of cytotoxic T lymphocytes (CTLs) against weakly immunogenic tumor-associated antigens (TAAs). A critical issue is...

Human Non-Germinal Center B Cell Interleukin (IL)-12 Production Is Primarily Regulated by T Cell Signals CD40 Ligand, Interferon γ, and IL-10: Role of B Cells in the Maintenance of  T Cell Responses

Schultze, Joachim L., Michalak, Sabine, Lowne, Joel, Wong, Adam, Gilleece, Maria H., Gribben, John G., ...

Interleukin (IL)-12 is expressed mainly in antigen-presenting cells after challenge with microbial material or after CD40 activation. Although IL-12 was cloned from human Epstein-Barr virus...

Temporal genetic program following B-cell receptor cross-linking: altered balance between proliferation and death in healthy and malignant B cells

Vallat, Laurent D., Park, Yuhyun, Li, Cheng, Gribben, John G.

Gene expression in cells is a dynamic process but is usually examined at a single time point. We used gene expression profiling over time to build temporal models of gene transcription after B-cell...

Chronic lymphocytic leukemia T cells show impaired immunological synapse formation that can be reversed with an immunomodulating drug

Ramsay, Alan G., Johnson, Amy J., Lee, Abigail M., Gorgün, Güllü, Le Dieu, Rifca, Blum, William, ...

Cancer is associated with immune deficiency, but the biologic basis of this is poorly defined. Here we demonstrate that impaired actin polymerization results in CD4+ and CD8+ T cells from patients...

Quantitative analysis of minimal residual disease predicts relapse in children with B-lineage acute lymphoblastic leukemia in DFCI ALL Consortium Protocol 95-01

Zhou, Jianbiao, Goldwasser, Meredith A, Li, Aihong, Dahlberg, Suzanne E., Neuberg, Donna, Wang, Hongjun, ...

In a prospective trial in 284 children with B-lineage acute lymphoblastic leukemia (ALL), we assessed the clinical utility of real-time quantitative polymerase chain reaction analysis of antigen...

Nonstochastic pairing of immunoglobulin heavy and light chains expressed by chronic lymphocytic leukemia B cells is predicated on the heavy chain CDR3

Widhopf, George F., Goldberg, Craig J., Toy, Traci L., Rassenti, Laura Z., Wierda, William G., Byrd, John C., ...

We analyzed the immunoglobulin (Ig) variable heavy (IGHV) and variable light chain genes used by leukemia cells of 258 unrelated patients with chronic lymphocytic leukemia (CLL) found to express...

Use of IGHV3–21 in chronic lymphocytic leukemia is associated with high-risk disease and reflects antigen-driven, post–germinal center leukemogenic selection

Ghia, Emanuela M., Jain, Sonia, Widhopf, George F., Rassenti, Laura Z., Keating, Michael J., Wierda, William G., ...

We examined the chronic lymphocytic leukemia (CLL) cells of 2457 patients evaluated by the CLL Research Consortium (CRC) and found that 63 (2.6%) expressed immunoglobulin (Ig) encoded by the Ig...

SAGE analysis demonstrates increased expression of TOSO contributing to Fas-mediated resistance in CLL

Proto-Siqueira, Rodrigo, Panepucci, Rodrigo A., Careta, Francisco P., Lee, Abigail, Clear, Andrew, Morris, Kelly, ...

To identify novel genes involved in the molecular pathogenesis of chronic lymphocytic leukemia (CLL) we performed a serial analysis of gene expression (SAGE) in CLL cells, and compared this with...

Relative value of ZAP-70, CD38, and immunoglobulin mutation status in predicting aggressive disease in chronic lymphocytic leukemia

Rassenti, Laura Z., Jain, Sonia, Keating, Michael J., Wierda, William G., Grever, Michael R., Byrd, John C., ...

Leukemia-cell expression of ZAP-70, CD38, or unmutated immunoglobulin heavy chain variable region genes (U-IGHV) each is associated with aggressive disease in patients with chronic lymphocytic...

Outcome of alloanergized haploidentical bone marrow transplantation after ex vivo costimulatory blockade: results of 2 phase 1 studies

Davies, Jeff K., Gribben, John G., Brennan, Lisa L., Yuk, Dongin, Nadler, Lee M., Guinan, Eva C.

We report the outcomes of 24 patients with high-risk hematologic malignancies or bone marrow failure (BMF) who received haploidentical bone marrow transplantation (BMT) after ex vivo induction of...

Immune dysfunction in chronic lymphocytic leukemia T cells and lenalidomide as an immunomodulatory drug

Ramsay, Alan G., Gribben, John G.

A new agent that is being used in chronic lymphocytic leukemia (CLL) and is receiving considerable interest is the immunomodulatory drug lenalidomide. The precise anti-CLL mechanism of action of...

Eμ-TCL1 mice represent a model for immunotherapeutic reversal of chronic lymphocytic leukemia-induced T-cell dysfunction

Gorgun, Gullu, Ramsay, Alan G., Holderried, Tobias A. W., Zahrieh, David, Le Dieu, Rifca, Liu, Fenglong, ...

Preclinical animal models have largely ignored the immune-suppressive mechanisms that are important in human cancers. The identification and use of such models should allow better predictions of...