John Hiscott

Hepatitis C virus NS2 and NS3/4A proteins are potent inhibitors of host cell cytokine/chemokine gene expression (2006)

Kaukinen, Pasi, Sillanpää, Maarit, Kotenko, Sergei, Lin, Rongtuan, Hiscott, John, Melén, Krister, ...

Abstract Background Hepatitis C virus (HCV) encodes several proteins that interfere with the host cell antiviral response. Previously, the serine protease NS3/4A was shown to inhibit IFN-β gene...

Structural and Functional Analysis of Interferon Regulatory Factor 3: Localization of the Transactivation and Autoinhibitory Domains

Lin, Rongtuan, Mamane, Yael, Hiscott, John

The interferon regulatory factor 3 (IRF-3) gene encodes a 55-kDa protein which is expressed constitutively in all tissues. In unstimulated cells, IRF-3 is present in an inactive cytoplasmic form;...

Identification by In Vivo Genomic Footprinting of a Transcriptional Switch Containing NF-κB and Sp1 That Regulates the IκBα Promoter

Algarté, Michèle, Kwon, Hakju, Génin, Pierre, Hiscott, John

In unstimulated cells, NF-κB transcription factors are retained in the cytoplasm by inhibitory IκB proteins. Upon stimulation by multiple inducers including cytokines or viruses, IκBα is rapidly...

Selective DNA Binding and Association with the CREB Binding Protein Coactivator Contribute to Differential Activation of Alpha/Beta Interferon Genes by Interferon Regulatory Factors 3 and 7

Lin, Rongtuan, Génin, Pierre, Mamane, Yaël, Hiscott, John

Recent studies implicate the interferon (IFN) regulatory factors (IRF) IRF-3 and IRF-7 as key activators of the alpha/beta IFN (IFN-α/β) genes as well as the RANTES chemokine gene. Using...

IκB-Mediated Inhibition of Virus-Induced Beta Interferon Transcription

Algarté, Michèle, Nguyen, Hannah, Heylbroeck, Christophe, Lin, Rongtuan, Hiscott, John

We have examined the consequences of overexpression of the IκBα and IκBβ inhibitory proteins on the regulation of NF-κB-dependent beta interferon (IFN-β) gene transcription in human cells after...

Virus-Dependent Phosphorylation of the IRF-3 Transcription Factor Regulates Nuclear Translocation, Transactivation Potential, and Proteasome-Mediated Degradation

Lin, Rongtuan, Heylbroeck, Christophe, Pitha, Paula M., Hiscott, John

The interferon regulatory factors (IRF) consist of a growing family of related transcription proteins first identified as regulators of the alpha beta interferon (IFN-α/β) gene promoters, as well...

The IRF-3 Transcription Factor Mediates Sendai Virus-Induced Apoptosis

Heylbroeck, Christophe, Balachandran, Siddharth, Servant, Marc J., DeLuca, Carmela, Barber, Glen N., Lin, Rongtuan, ...

Virus infection of target cells can result in different biological outcomes: lytic infection, cellular transformation, or cell death by apoptosis. Cells respond to virus infection by the activation...

Essential Role of Interferon Regulatory Factor 3 in Direct Activation of RANTES Chemokine Transcription

Lin, Rongtuan, Heylbroeck, Christophe, Genin, Pierre, Pitha, Paula M., Hiscott, John

Localized and systemic cytokine production in virus-infected cells play an important role in the outcome of viral infection and pathogenicity. Activation of the interferon regulatory factors (IRF) in...

Recognition of the Measles Virus Nucleocapsid as a Mechanism of IRF-3 Activation

TenOever, Benjamin R., Servant, Marc J., Grandvaux, Nathalie, Lin, Rongtuan, Hiscott, John

The mechanisms of cellular recognition for virus infection remain poorly understood despite the wealth of information regarding the signaling events and transcriptional responses that ensue. Host...

Transcriptional Profiling of Interferon Regulatory Factor 3 Target Genes: Direct Involvement in the Regulation of Interferon-Stimulated Genes

Grandvaux, Nathalie, Servant, Marc J., TenOever, Benjamin, Sen, Ganes C., Balachandran, Siddarth, Barber, Glen N., ...

Ubiquitously expressed interferon regulatory factor 3 (IRF-3) is directly activated after virus infection and functions as a key activator of the immediate-early alpha/beta interferon (IFN) genes, as...

Activation of TBK1 and IKKɛ Kinases by Vesicular Stomatitis Virus Infection and the Role of Viral Ribonucleoprotein in the Development of Interferon Antiviral Immunity

TenOever, Benjamin R., Sharma, Sonia, Zou, Wen, Sun, Qiang, Grandvaux, Nathalie, Julkunen, Ilkka, ...

Mounting an immune response to a viral pathogen involves the initial recognition of viral antigens through Toll-like receptor-dependent and -independent pathways and the subsequent triggering of...

Inhibition of RIG-I-Dependent Signaling to the Interferon Pathway during Hepatitis C Virus Expression and Restoration of Signaling by IKKɛ

Breiman, Adrien, Grandvaux, Nathalie, Lin, Rongtuan, Ottone, Catherine, Akira, Shizuo, Yoneyama, Mitsutoshi, ...

Interferon (IFN) is one important effector of the innate immune response, induced by different viral or bacterial components through Toll-like receptor (TLR)-dependent and -independent mechanisms. As...

Gene Expression and Antiviral Activity of Alpha/Beta Interferons and Interleukin-29 in Virus-Infected Human Myeloid Dendritic Cells

Österlund, Pamela, Veckman, Ville, Sirén, Jukka, Klucher, Kevin M., Hiscott, John, Matikainen, Sampsa, ...

Dendritic cells (DCs) respond to microbial infections by undergoing phenotypic maturation and by producing multiple cytokines. In the present study, we analyzed the ability of influenza A and Sendai...

Regulation of Human Immunodeficiency Virus Type 1 Gene Expression by Clade-Specific Tat Proteins

Desfosses, Yan, Solis, Mayra, Sun, Qiang, Grandvaux, Nathalie, Van Lint, Carine, Burny, Arsene, ...

The major group of human immunodeficiency virus type 1 (HIV-1) strains that comprise the current global pandemic have diversified during their worldwide spread into at least 10 distinct subtypes, or...

Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32

Berro, Reem, Kehn, Kylene, De La Fuente, Cynthia, Pumfery, Anne, Adair, Richard, Wade, John, ...

The human immunodeficiency virus type 1 (HIV-1) potent transactivator Tat protein mediates pleiotropic effects on various cell functions. Posttranslational modification of Tat affects its activity...

Tumor Necrosis Factor Alpha Enhances Influenza A Virus-Induced Expression of Antiviral Cytokines by Activating RIG-I Gene Expression

Matikainen, Sampsa, Sirén, Jukka, Tissari, Jorma, Veckman, Ville, Pirhonen, Jaana, Severa, Martina, ...

Epithelial cells of the lung are the primary targets for respiratory viruses. Virus-carried single-stranded RNA (ssRNA) can activate Toll-like receptors (TLRs) 7 and 8, whereas dsRNA is bound by TLR3...

Dissociation of a MAVS/IPS-1/VISA/Cardif-IKKɛ Molecular Complex from the Mitochondrial Outer Membrane by Hepatitis C Virus NS3-4A Proteolytic Cleavage†

Lin, Rongtuan, Lacoste, Judith, Nakhaei, Peyman, Sun, Qiang, Yang, Long, Paz, Suzanne, ...

Intracellular RNA virus infection is detected by the cytoplasmic RNA helicase RIG-I that plays an essential role in signaling to the host antiviral response. Recently, the adapter molecule that links...

Structural and Functional Analysis of Interferon Regulatory Factor 3: Localization of the Transactivation and Autoinhibitory Domains

Lin, Rongtuan, Mamane, Yael, Hiscott, John

The interferon regulatory factor 3 (IRF-3) gene encodes a 55-kDa protein which is expressed constitutively in all tissues. In unstimulated cells, IRF-3 is present in an inactive cytoplasmic form;...

Identification by In Vivo Genomic Footprinting of a Transcriptional Switch Containing NF-κB and Sp1 That Regulates the IκBα Promoter

Algarté, Michèle, Kwon, Hakju, Génin, Pierre, Hiscott, John

In unstimulated cells, NF-κB transcription factors are retained in the cytoplasm by inhibitory IκB proteins. Upon stimulation by multiple inducers including cytokines or viruses, IκBα is rapidly...

Selective DNA Binding and Association with the CREB Binding Protein Coactivator Contribute to Differential Activation of Alpha/Beta Interferon Genes by Interferon Regulatory Factors 3 and 7

Lin, Rongtuan, Génin, Pierre, Mamane, Yaël, Hiscott, John

Recent studies implicate the interferon (IFN) regulatory factors (IRF) IRF-3 and IRF-7 as key activators of the alpha/beta IFN (IFN-α/β) genes as well as the RANTES chemokine gene. Using...

IκB-Mediated Inhibition of Virus-Induced Beta Interferon Transcription

Algarté, Michèle, Nguyen, Hannah, Heylbroeck, Christophe, Lin, Rongtuan, Hiscott, John

We have examined the consequences of overexpression of the IκBα and IκBβ inhibitory proteins on the regulation of NF-κB-dependent beta interferon (IFN-β) gene transcription in human cells after...

Virus-Dependent Phosphorylation of the IRF-3 Transcription Factor Regulates Nuclear Translocation, Transactivation Potential, and Proteasome-Mediated Degradation

Lin, Rongtuan, Heylbroeck, Christophe, Pitha, Paula M., Hiscott, John

The interferon regulatory factors (IRF) consist of a growing family of related transcription proteins first identified as regulators of the alpha beta interferon (IFN-α/β) gene promoters, as well...

The IRF-3 Transcription Factor Mediates Sendai Virus-Induced Apoptosis

Heylbroeck, Christophe, Balachandran, Siddharth, Servant, Marc J., DeLuca, Carmela, Barber, Glen N., Lin, Rongtuan, ...

Virus infection of target cells can result in different biological outcomes: lytic infection, cellular transformation, or cell death by apoptosis. Cells respond to virus infection by the activation...

Essential Role of Interferon Regulatory Factor 3 in Direct Activation of RANTES Chemokine Transcription

Lin, Rongtuan, Heylbroeck, Christophe, Genin, Pierre, Pitha, Paula M., Hiscott, John

Localized and systemic cytokine production in virus-infected cells play an important role in the outcome of viral infection and pathogenicity. Activation of the interferon regulatory factors (IRF) in...

Recognition of the Measles Virus Nucleocapsid as a Mechanism of IRF-3 Activation

TenOever, Benjamin R., Servant, Marc J., Grandvaux, Nathalie, Lin, Rongtuan, Hiscott, John

The mechanisms of cellular recognition for virus infection remain poorly understood despite the wealth of information regarding the signaling events and transcriptional responses that ensue. Host...

Transcriptional Profiling of Interferon Regulatory Factor 3 Target Genes: Direct Involvement in the Regulation of Interferon-Stimulated Genes

Grandvaux, Nathalie, Servant, Marc J., TenOever, Benjamin, Sen, Ganes C., Balachandran, Siddarth, Barber, Glen N., ...

Ubiquitously expressed interferon regulatory factor 3 (IRF-3) is directly activated after virus infection and functions as a key activator of the immediate-early alpha/beta interferon (IFN) genes, as...

Activation of TBK1 and IKKɛ Kinases by Vesicular Stomatitis Virus Infection and the Role of Viral Ribonucleoprotein in the Development of Interferon Antiviral Immunity

TenOever, Benjamin R., Sharma, Sonia, Zou, Wen, Sun, Qiang, Grandvaux, Nathalie, Julkunen, Ilkka, ...

Mounting an immune response to a viral pathogen involves the initial recognition of viral antigens through Toll-like receptor-dependent and -independent pathways and the subsequent triggering of...

Inhibition of RIG-I-Dependent Signaling to the Interferon Pathway during Hepatitis C Virus Expression and Restoration of Signaling by IKKɛ

Breiman, Adrien, Grandvaux, Nathalie, Lin, Rongtuan, Ottone, Catherine, Akira, Shizuo, Yoneyama, Mitsutoshi, ...

Interferon (IFN) is one important effector of the innate immune response, induced by different viral or bacterial components through Toll-like receptor (TLR)-dependent and -independent mechanisms. As...

Regulation of Human Immunodeficiency Virus Type 1 Gene Expression by Clade-Specific Tat Proteins

Desfosses, Yan, Solis, Mayra, Sun, Qiang, Grandvaux, Nathalie, Van Lint, Carine, Burny, Arsene, ...

The major group of human immunodeficiency virus type 1 (HIV-1) strains that comprise the current global pandemic have diversified during their worldwide spread into at least 10 distinct subtypes, or...

Gene Expression and Antiviral Activity of Alpha/Beta Interferons and Interleukin-29 in Virus-Infected Human Myeloid Dendritic Cells

Österlund, Pamela, Veckman, Ville, Sirén, Jukka, Klucher, Kevin M., Hiscott, John, Matikainen, Sampsa, ...

Dendritic cells (DCs) respond to microbial infections by undergoing phenotypic maturation and by producing multiple cytokines. In the present study, we analyzed the ability of influenza A and Sendai...

Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32

Berro, Reem, Kehn, Kylene, De La Fuente, Cynthia, Pumfery, Anne, Adair, Richard, Wade, John, ...

The human immunodeficiency virus type 1 (HIV-1) potent transactivator Tat protein mediates pleiotropic effects on various cell functions. Posttranslational modification of Tat affects its activity...

Tumor Necrosis Factor Alpha Enhances Influenza A Virus-Induced Expression of Antiviral Cytokines by Activating RIG-I Gene Expression

Matikainen, Sampsa, Sirén, Jukka, Tissari, Jorma, Veckman, Ville, Pirhonen, Jaana, Severa, Martina, ...

Epithelial cells of the lung are the primary targets for respiratory viruses. Virus-carried single-stranded RNA (ssRNA) can activate Toll-like receptors (TLRs) 7 and 8, whereas dsRNA is bound by TLR3...

Dissociation of a MAVS/IPS-1/VISA/Cardif-IKKɛ Molecular Complex from the Mitochondrial Outer Membrane by Hepatitis C Virus NS3-4A Proteolytic Cleavage†

Lin, Rongtuan, Lacoste, Judith, Nakhaei, Peyman, Sun, Qiang, Yang, Long, Paz, Suzanne, ...

Intracellular RNA virus infection is detected by the cytoplasmic RNA helicase RIG-I that plays an essential role in signaling to the host antiviral response. Recently, the adapter molecule that links...

A type I interferon autocrine–paracrine loop is involved in Toll-like receptor-induced interleukin-12p70 secretion by dendritic cells

Gautier, Grégory, Humbert, Martine, Deauvieau, Florence, Scuiller, Mathieu, Hiscott, John, Bates, Elizabeth E.M., ...

Dendritic cells (DC) produce interleukin-12 (IL-12) in response to Toll-like receptor (TLR) activation. Two major TLR signaling pathways participate in the response to pathogens: the nuclear...

Vesicular Stomatitis Virus Oncolysis of T Lymphocytes Requires Cell Cycle Entry and Translation Initiation▿ †

Oliere, Stephanie, Arguello, Meztli, Mesplede, Thibault, Tumilasci, Vanessa, Nakhaei, Peyman, Stojdl, David, ...

Vesicular stomatitis virus (VSV) is a candidate oncolytic virus that replicates and induces cell death in cancer cells while sparing normal cells. Although defects in the interferon antiviral...