Sequencing Multimegabase-Template DNA with BigDye Terminator Chemistry (1998)
Heiner, Cheryl R., Hunkapiller, Kathryn L., Chen, Shiaw-Min, Glass, John I., Chen, Ellson Y.
Genome of the Bacterium Streptococcus pneumoniae Strain R6
Hoskins, JoAnn, Alborn, William E., Arnold, Jeffrey, Blaszczak, Larry C., Burgett, Stanley, DeHoff, Bradley S., ...
Streptococcus pneumoniae is among the most significant causes of bacterial disease in humans. Here we report the 2,038,615-bp genomic sequence of the gram-positive bacterium S. pneumoniae R6. Because...
Sequencing Multimegabase-Template DNA with BigDye Terminator Chemistry
Heiner, Cheryl R., Hunkapiller, Kathryn L., Chen, Shiaw-Min, Glass, John I., Chen, Ellson Y.
Using the recently introduced BigDye™ terminators, large-template DNA can be directly sequenced with custom primers on automated instruments. Cycle sequencing conditions are presented to sequence...
Pyruvate Oxidase Is a Determinant of Avery's Rough Morphology
Belanger, Aimee E., Clague, Melissa J., Glass, John I., LeBlanc, Donald J.
In pioneering studies, Avery et al. identified DNA as the hereditary material (A. T. Avery, C. M. MacLeod, and M. McCarty, J. Exp. Med. 79:137-158, 1944). They demonstrated, by means of variation in...
Essential genes of a minimal bacterium
Glass, John I., Assad-Garcia, Nacyra, Alperovich, Nina, Yooseph, Shibu, Lewis, Matthew R., Maruf, Mahir, ...
Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be...
Genome of the Bacterium Streptococcus pneumoniae Strain R6
Hoskins, JoAnn, Alborn, William E., Arnold, Jeffrey, Blaszczak, Larry C., Burgett, Stanley, DeHoff, Bradley S., ...
Streptococcus pneumoniae is among the most significant causes of bacterial disease in humans. Here we report the 2,038,615-bp genomic sequence of the gram-positive bacterium S. pneumoniae R6. Because...
Sequencing Multimegabase-Template DNA with BigDye Terminator Chemistry
Heiner, Cheryl R., Hunkapiller, Kathryn L., Chen, Shiaw-Min, Glass, John I., Chen, Ellson Y.
Using the recently introduced BigDye™ terminators, large-template DNA can be directly sequenced with custom primers on automated instruments. Cycle sequencing conditions are presented to sequence...
Pyruvate Oxidase Is a Determinant of Avery's Rough Morphology
Belanger, Aimee E., Clague, Melissa J., Glass, John I., LeBlanc, Donald J.
In pioneering studies, Avery et al. identified DNA as the hereditary material (A. T. Avery, C. M. MacLeod, and M. McCarty, J. Exp. Med. 79:137-158, 1944). They demonstrated, by means of variation in...
Essential genes of a minimal bacterium
Glass, John I., Assad-Garcia, Nacyra, Alperovich, Nina, Yooseph, Shibu, Lewis, Matthew R., Maruf, Mahir, ...
Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be...
Lanie, Joel A., Ng, Wai-Leung, Kazmierczak, Krystyna M., Andrzejewski, Tiffany M., Davidsen, Tanja M., Wayne, Kyle J., ...
Streptococcus pneumoniae (pneumococcus) is a leading human respiratory pathogen that causes a variety of serious mucosal and invasive diseases. D39 is an historically important serotype 2 strain that...
Williamson, Shannon J., Rusch, Douglas B., Yooseph, Shibu, Halpern, Aaron L., Heidelberg, Karla B., Glass, John I., ...
Viruses are the most abundant biological entities on our planet. Interactions between viruses and their hosts impact several important biological processes in the world's oceans such as horizontal...
A Genome-Scale Metabolic Reconstruction of Mycoplasma genitalium, iPS189
Suthers, Patrick F., Dasika, Madhukar S., Kumar, Vinay Satish, Denisov, Gennady, Glass, John I., Maranas, Costas D.
With a genome size of ∼580 kb and approximately 480 protein coding regions, Mycoplasma genitalium is one of the smallest known self-replicating organisms and, additionally, has extremely fastidious...