Vingadassalom, Didier F., Kazlauskas, Arunas, Skehan, Brian M., Cheng, Hui-Chun, Magoun, Loranne, Robbins, Douglas, ...
Enterohemorrhagic Escherichia coli O157:H7 translocates 2 effectors to trigger localized actin assembly in mammalian cells, resulting in filamentous actin "pedestals." One effector, the...
Targeting type III secretion in Yersinia pestis (2008)
Pan, Ning, Brady, Michael John, Leong, John M., Goguen, Jon D.
Yersinia pestis, the causative agent of plague, utilizes a plasmid-encoded type III secretion system (T3SS) to aid it with its resistance to host defenses. This system injects a set of effector...
Hierarchical regulation of WASP/WAVE proteins (2008)
Padrick, Shae B., Cheng, Hui-Chun, Ismail, Ayman M., Panchal, Sanjay C., Doolittle, Lynda K., Kim, Soyeon, ...
Members of the Wiskott-Aldrich syndrome protein (WASP) family control actin dynamics in eukaryotic cells by stimulating the actin nucleating activity of the Arp2/3 complex. The prevailing paradigm...
Campellone, Kenneth Geno, Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D., McGhie, Emma J., Hayward, Richard D., ...
Enterohemorrhagic Escherichia coli (EHEC) generate F-actin-rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along...
Chen, Qiang, Fischer, Joshua Richard, Benoit, Vivian M., Dufour, Nicholas P., Youderian, Philip, Leong, John M.
Borrelia burgdorferi is the causative agent of Lyme disease, the most common vector-borne illness in the Northern hemisphere. Low-passage-number infectious strains of B. burgdorferi exhibit extremely...
Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U) (2008)
Cheng, Hui-Chun, Skehan, Brian M., Campellone, Kenneth Geno, Leong, John M., Rosen, Michael K.
During infection, enterohaemorrhagic Escherichia coli (EHEC) takes over the actin cytoskeleton of eukaryotic cells by injecting the EspF(U) protein into the host cytoplasm. EspF(U) controls actin by...
Ritchie, Jennifer M., Brady, Michael J., Riley, Kathleen N., Ho, Theresa Deland, Campellone, Kenneth Geno, Herman, Ira M., ...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 induces filamentous actin-rich 'pedestals' on intestinal epithelial cells. Pedestal formation in vitro requires translocation of bacterial effectors...
Brady, Michael J., Campellone, Kenneth Geno, Ghildiyal, Megha, Leong, John M.
The Tir proteins of enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC respectively) are each translocated into the host plasma membrane where they promote F-actin pedestals in...
Campellone, Kenneth Geno, Roe, Andrew J., Lobner-Olesen, Anders, Murphy, Kenan C., Magoun, Loranne, Brady, Michael J., ...
Enterohaemorrhagic Escherichia coli (EHEC) are highly infectious pathogens capable of causing severe diarrhoeal illnesses. As a critical step during their colonization, EHEC adhere intimately to...
Campellone, Kenneth Geno, Brady, Michael J., Alamares, Judith G., Rowe, Daniel C., Skehan, Brian M., Tipper, Donald J., ...
Enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) both utilize type III secretion systems that translocate the effector protein Tir into the plasma membrane of mammalian...
Exploiting pathogenic Escherichia coli to model transmembrane receptor signalling (2006)
Hayward, Richard D., Leong, John M., Koronakis, Vassilis, Campellone, Kenneth Geno
Many microbial pathogens manipulate the actin cytoskeleton of eukaryotic target cells to promote their internalization, intracellular motility and dissemination. Enteropathogenic and...
Fischer, Joshua Richard, LeBlanc, Kimberly T., Leong, John M.
Borrelia burgdorferi, the agent of Lyme disease, causes a multisystemic illness that can affect the skin, heart, joints, and nervous system and is capable of attachment to diverse cell types. Among...
Coburn, Jenifer, Fischer, Joshua R., Leong, John M.
The Lyme disease spirochetes, comprised of at least three closely related species, Borrelia burgdorferi, Borrelia garinii and Borrelia afzelii, are fascinating and enigmatic bacterial pathogens. They...
Campellone, Kenneth Geno, Leong, John M.
Enteropathogenic Escherichia coli (EPEC) stimulates tyrosine-kinase signalling cascades to trigger localized actin assembly within mammalian cells. During actin 'pedestal' formation, the EPEC...
Campellone, Kenneth Geno, Robbins, Douglas, Leong, John M.
Several microbial pathogens including enteropathogenic E. coli (EPEC) exploit mammalian tyrosine-kinase signaling cascades to recruit Nck adaptor proteins and activate N-WASP-Arp2/3-mediated actin...
Campellone, Kenneth Geno, Rankin, Susannah, Pawson, Tony, Kirschner, Marc W., Tipper, Donald J., Leong, John M.
Enteropathogenic Escherichia coli (EPEC) translocates effector proteins into mammalian cells to promote reorganization of the cytoskeleton into filamentous actin pedestals. One effector, Tir, is a...
Spirochete-platelet attachment and thrombocytopenia in murine relapsing fever borreliosis (2003)
Alugupalli, Kishore R., Michelson, Alan D., Joris, Isabelle, Schwan, Tom G., Hodivala-Dilke, Kairbaan, Hynes, Richard O., ...
Thrombocytopenia is common in persons infected with relapsing fever Borreliae. We previously showed that the relapsing fever spirochete Borrelia hermsii binds to and activates human platelets in...
Fischer, Joshua Richard, Parveen, Nikhat, Magoun, Loranne, Leong, John M.
Host cell binding is an essential step in colonization by many bacterial pathogens, and the Lyme disease agent, Borrelia burgdorferi, which colonizes multiple tissues, is capable of attachment to...
Alugupalli, Kishore R., Gerstein, Rachel M., Chen, Jianzhu, Szomolanyi-Tsuda, Eva, Woodland, Robert T., Leong, John M.
The rate of pathogen clearance is a critical determinant of morbidity and mortality. We sought to characterize the immune response responsible for the remarkably rapid clearance of individual...
Campellone, Kenneth Geno, Leong, John M.
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli O157:H7 (EHEC) form characteristic lesions on infected mammalian cells called actin pedestals. Each of these two pathogens...
Liu, Hui, Radhakrishnan, Padhma, Magoun, Loranne, Prabu-Jeyabalan, Moses, Campellone, Kenneth Geno, Savage, Pamela Joyce, ...
Attachment to host cells by enterohaemorrhagic Escherichia coli (EHEC) is associated with the formation of a highly organized cytoskeletal structure containing filamentous actin, termed an attaching...
Campellone, Kenneth Geno, Giese, Andrew, Tipper, Donald J., Leong, John M.
Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) each promote the reorganization of actin into filamentous pedestal structures beneath attached bacteria during...
Liu, Hui, Magoun, Loranne, Luperchio, Steven A., Schauer, David B., Leong, John M.
Enterohaemorrhagic Escherichia coli (EHEC) has emerged as an important agent of diarrhoeal disease. Attachment to host cells, an essential step during intestinal colonization by EHEC, is associated...
Parveen, Nikhat, Robbins, Douglas, Leong, John M.
Lyme disease, a chronic multisystemic disorder that can affect the skin, heart, joints, and nervous system is caused by Borrelia burgdorferi sensu lato. Lyme disease spirochetes were previously shown...
Liu, Hui, Magoun, Loranne, Leong, John M.
Intimin is a bacterial outer membrane protein required for intimate attachment of enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) to mammalian cells. beta1-chain integrins...
Leong, John M., Robbins, Douglas, Rosenfeld, Louis, Lahiri, Biswajit, Parveen, Nikhat
Borrelia burgdorferi, the Lyme disease agent, binds glycosaminoglycans (GAGs) such as heparin, heparan sulfate, and dermatan sulfate. Heparin or heparan sulfate fractions separated by size or charge...
Leong, John M., Wang, Hong, Magoun, Loranne, Field, Jodie A., Morrissey, Pamela E., Robbins, Douglas, ...
The Lyme disease spirochete, Borrelia burgdorferi, infects multiple tissues, such as the heart, joint, skin, and nervous system and has been shown to recognize heparan sulfate and dermatan sulfate...
Stockbauer, Kathryn E., Magoun, Loranne, Liu, Mengyao, Burns, Eugene H., Gubba, Siddeswar, Renish, Sarah, ...
The human pathogenic bacterium group A Streptococcus produces an extracellular cysteine protease [streptococcal pyrogenic exotoxin B (SpeB)] that is a critical virulence factor for invasive disease...
Parveen, Nikhat, Robbins, Douglas, Leong, John M.
Lyme disease, a chronic multisystemic disorder that can affect the skin, heart, joints, and nervous system is caused by Borrelia burgdorferi sensu lato. Lyme disease spirochetes were previously shown...
Liu, Hui, Magoun, Loranne, Leong, John M.
Intimin is a bacterial outer membrane protein required for intimate attachment of enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) to mammalian cells. β1-chain integrins have...
Leong, John M., Wang, Hong, Magoun, Loranne, Field, Jodie A., Morrissey, Pamela E., Robbins, Douglas, ...
The Lyme disease spirochete, Borrelia burgdorferi, infects multiple tissues, such as the heart, joint, skin, and nervous system and has been shown to recognize heparan sulfate and dermatan sulfate...
Integrins αvβ3 and α5β1 Mediate Attachment of Lyme Disease Spirochetes to Human Cells
Coburn, Jenifer, Magoun, Loranne, Bodary, Sarah C., Leong, John M.
Borrelia burgdorferi (sensu lato), the agent of Lyme disease, is able to cause chronic, multisystemic infections in human and animal hosts. Attachment of the spirochete to host cells is likely to be...
Leong, John M., Robbins, Douglas, Rosenfeld, Louis, Lahiri, Biswajit, Parveen, Nikhat
Borrelia burgdorferi, the Lyme disease agent, binds glycosaminoglycans (GAGs) such as heparin, heparan sulfate, and dermatan sulfate. Heparin or heparan sulfate fractions separated by size or charge...
Lei, Benfang, DeLeo, Frank R., Reid, Sean D., Voyich, Jovanka M., Magoun, Loranne, Liu, Mengyao, ...
Recently, it was reported that a streptococcal Mac protein (designated Mac5005) made by serotype M1 group A Streptococcus (GAS) is a homologue of human CD11b that inhibits opsonophagocytosis and...
Fischer, Joshua R., Parveen, Nikhat, Magoun, Loranne, Leong, John M.
Host cell binding is an essential step in colonization by many bacterial pathogens, and the Lyme disease agent, Borrelia burgdorferi, which colonizes multiple tissues, is capable of attachment to...
Relapsing Fever Spirochetes Contain Chromosomal Genes with Unique Direct Tandemly Repeated Sequences
Guyard, Cyril, Chester, Earl M., Raffel, Sandra J., Schrumpf, Merry E., Policastro, Paul F., Porcella, Stephen F., ...
Genome sequencing of the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae identified three open reading frames (ORFs) on the chromosomes that contained internal, tandemly repeated...
Fischer, Joshua R., LeBlanc, Kimberly T., Leong, John M.
Borrelia burgdorferi, the agent of Lyme disease, causes a multisystemic illness that can affect the skin, heart, joints, and nervous system and is capable of attachment to diverse cell types. Among...
Parveen, Nikhat, Cornell, Kenneth A., Bono, James L., Chamberland, Christen, Rosa, Patricia, Leong, John M.
Bgp, one of the surface-localized glycosaminoglycan-binding proteins of the Lyme disease spirochete, Borrelia burgdorferi, exhibited nucleosidase activity. Infection of SCID mice with B. burgdorferi...
Stockbauer, Kathryn E., Magoun, Loranne, Liu, Mengyao, Burns, Eugene H., Gubba, Siddeswar, Renish, Sarah, ...
The human pathogenic bacterium group A Streptococcus produces an extracellular cysteine protease [streptococcal pyrogenic exotoxin B (SpeB)] that is a critical virulence factor for invasive disease...
Parveen, Nikhat, Robbins, Douglas, Leong, John M.
Lyme disease, a chronic multisystemic disorder that can affect the skin, heart, joints, and nervous system is caused by Borrelia burgdorferi sensu lato. Lyme disease spirochetes were previously shown...
Liu, Hui, Magoun, Loranne, Leong, John M.
Intimin is a bacterial outer membrane protein required for intimate attachment of enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) to mammalian cells. β1-chain integrins have...
Leong, John M., Wang, Hong, Magoun, Loranne, Field, Jodie A., Morrissey, Pamela E., Robbins, Douglas, ...
The Lyme disease spirochete, Borrelia burgdorferi, infects multiple tissues, such as the heart, joint, skin, and nervous system and has been shown to recognize heparan sulfate and dermatan sulfate...
Integrins αvβ3 and α5β1 Mediate Attachment of Lyme Disease Spirochetes to Human Cells
Coburn, Jenifer, Magoun, Loranne, Bodary, Sarah C., Leong, John M.
Borrelia burgdorferi (sensu lato), the agent of Lyme disease, is able to cause chronic, multisystemic infections in human and animal hosts. Attachment of the spirochete to host cells is likely to be...
Leong, John M., Robbins, Douglas, Rosenfeld, Louis, Lahiri, Biswajit, Parveen, Nikhat
Borrelia burgdorferi, the Lyme disease agent, binds glycosaminoglycans (GAGs) such as heparin, heparan sulfate, and dermatan sulfate. Heparin or heparan sulfate fractions separated by size or charge...
Lei, Benfang, DeLeo, Frank R., Reid, Sean D., Voyich, Jovanka M., Magoun, Loranne, Liu, Mengyao, ...
Recently, it was reported that a streptococcal Mac protein (designated Mac5005) made by serotype M1 group A Streptococcus (GAS) is a homologue of human CD11b that inhibits opsonophagocytosis and...
Fischer, Joshua R., Parveen, Nikhat, Magoun, Loranne, Leong, John M.
Host cell binding is an essential step in colonization by many bacterial pathogens, and the Lyme disease agent, Borrelia burgdorferi, which colonizes multiple tissues, is capable of attachment to...
Relapsing Fever Spirochetes Contain Chromosomal Genes with Unique Direct Tandemly Repeated Sequences
Guyard, Cyril, Chester, Earl M., Raffel, Sandra J., Schrumpf, Merry E., Policastro, Paul F., Porcella, Stephen F., ...
Genome sequencing of the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae identified three open reading frames (ORFs) on the chromosomes that contained internal, tandemly repeated...
Fischer, Joshua R., LeBlanc, Kimberly T., Leong, John M.
Borrelia burgdorferi, the agent of Lyme disease, causes a multisystemic illness that can affect the skin, heart, joints, and nervous system and is capable of attachment to diverse cell types. Among...
Parveen, Nikhat, Cornell, Kenneth A., Bono, James L., Chamberland, Christen, Rosa, Patricia, Leong, John M.
Bgp, one of the surface-localized glycosaminoglycan-binding proteins of the Lyme disease spirochete, Borrelia burgdorferi, exhibited nucleosidase activity. Infection of SCID mice with B. burgdorferi...
Campellone, Kenneth G., Rankin, Susannah, Pawson, Tony, Kirschner, Marc W., Tipper, Donald J., Leong, John M.
Enteropathogenic Escherichia coli (EPEC) translocates effector proteins into mammalian cells to promote reorganization of the cytoskeleton into filamentous actin pedestals. One effector, Tir, is a...
Campellone, Kenneth G., Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D., McGhie, Emma J., Hayward, Richard D., ...
Enterohemorrhagic Escherichia coli (EHEC) generate F-actin–rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along...
Mousnier, Aurelie, Whale, Andrew D., Schüller, Stephanie, Leong, John M., Phillips, Alan D., Frankel, Gad
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important human pathogen that colonizes the gut mucosa via attaching and effacing (A/E) lesions; A/E lesion formation in vivo and ex vivo is...
Borrelia burgdorferi Lacking DbpBA Exhibits an Early Survival Defect during Experimental Infection▿
Weening, Eric H., Parveen, Nikhat, Trzeciakowski, Jerome P., Leong, John M., Höök, Magnus, Skare, Jonathan T.
Several Borrelia burgdorferi genes induced under mammalian host conditions have been purported to be important in Lyme disease pathogenesis based on their binding to host structures. These genes...
Chen, Qiang, Fischer, Joshua R., Benoit, Vivian M., Dufour, Nicholas P., Youderian, Philip, Leong, John M.
Borrelia burgdorferi is the causative agent of Lyme disease, the most common vector-borne illness in the Northern hemisphere. Low-passage-number infectious strains of B. burgdorferi exhibit extremely...
Targeting Type III Secretion in Yersinia pestis▿ †
Pan, Ning J., Brady, Michael J., Leong, John M., Goguen, Jon D.
Yersinia pestis, the causative agent of plague, utilizes a plasmid-encoded type III secretion system (T3SS) to aid it with its resistance to host defenses. This system injects a set of effector...
Vingadassalom, Didier, Kazlauskas, Arunas, Skehan, Brian, Cheng, Hui-Chun, Magoun, Loranne, Robbins, Douglas, ...
Enterohemorrhagic Escherichia coli O157:H7 translocates 2 effectors to trigger localized actin assembly in mammalian cells, resulting in filamentous actin “pedestals.” One effector, the...