1 Isolation and Identification of Bacteria from Ancient and Modern Ice Core Archives (2008)
Brent C. Christner, Ellen Mosley-thompson, Lonnie G. Thompson, Victor Zagorodnov, Kathleen S, John N. Reeve
Glacial ice traps and preserves soluble chemical species, gases and particulates including pollen grains, fungal spores and bacteria in chronologically-deposited archives. We have constructed an...
TrpY regulation of trpB2 transcription in Methanothermobacter thermautotrophicus (2008)
Karr, Elizabeth A., Sandman, Kathleen, Lurz, Rudi, Reeve, John N.
TrpY binds specifically to TRP box sequences upstream of trpB2, but the repression of trpB2 transcription requires additional TrpY assembly that is stimulated by but not dependent on the presence of...
Hirata, Akira, Kanai, Tamotsu, Santangelo, Thomas J., Tajiri, Momoko, Manabe, Kenji, Reeve, John N., ...
Polarity in archaeal operon transcription in Thermococcus kodakaraensis (2008)
Santangelo, Thomas J., Cubonova, L'ubomira, Matsumi, Rie, Atomi, Haruyuki, Imanaka, Tadayuki, Reeve, John N.
Copyright © 2005, American Society for Microbiology. All Rights Reserved. NOTES (2005)
Histones In Crenarchaea, Kathleen S, Steven J. Hallam, Edward F. Delong, John N. Reeve
Archaeal histone-encoding genes have been identified in marine Crenarchaea. The protein encoded by a representative of these genes, synthesized in vitro and expressed in Escherichia coli, binds DNA...
Use of a restriction enzyme-digested PCR product as substrate for helicase assays (2005)
Shin, Jae-Ho, Reeve, John N., Kelman, Zvi
DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not...
Marc,Frédéric, Sandman,Kathleen M., Lurz,Rudi, Reeve,John N.
DNA binding and the topology of DNA have been determined in complexes formed by >20 archaeal histone variants and archaeal histone dimer fusions with residue replacements at sites responsible for...
Reeve,John N., Rice,Jacqueline B.
There were 2 objectives in this research project: development of an in vivo assay for mistranslation-inducing activity of pollutants and characterization of amino acid substitutions. The first...
Marc, Frédéric, Sandman, Kathleen M., Lurz, Rudi, Reeve, John N.
DNA binding and the topology of DNA have been determined in complexes formed by >20 archaeal histone variants and archaeal histone dimer fusions with residue replacements at sites responsible for...
Weil, Clifford F., Beckler, Gregory S., Reeve, John N.
A restriction fragment of Methanococcus thermolithotrophicus genomic DNA was cloned into pUC8 to produce plasmid pET9301 which complements mutations in the hisA gene of Escherichia coli. Sequencing...
Molecular Biology of the Extremely Thermophilic Archaebacterium, Methanothermus Fervidus. (1998)
Genes encoding the subunit polypeptides of methyl coenzyme M reductase, tRNAs and rRNAs have been cloned and sequenced from the extremely thermophilic archaebacterium Methanothermus fervidus. The...
Molecular Biology of the Extremely Thermophilic Archaebacteria Methanothermus fervidus. (1998)
A DNA binding protein, designated HMf, and DNA dependent RNA polymerase (RNAP) have been isolated and characterized from the extremely thermophilic methanogen Methanothermus fervidus. Binding of HMf...
Complete Genome Sequence of Methanobacterium thermoautotrophicum ΔH: Functional . . . (1997)
Douglas R. Smith, Craig Deloughery, Hongmei Lee, Joann Dubois, ...
the ORF-encoded polypeptides are related to sequences with unknown functions, and 496 (27%) have little or no homology to sequences in public databases. Comparisons with Eucarya-, Bacteria-, and...
Transcription in Methanococcus vannielii /--by Aidan Noel Hennigan. (1993)
Advisor: John N. Reeve, Dept. of Microbiology.
Genes encoding stable RNAs in Methanothermus fervidus /--by Elizabeth S. Haas. (1989)
Advisor: John N. Reeve, MCD Biology Program.
Advisor: John N. Reeve, Dept. of Microbiology.
RNA polymerase binding sites and polyadenylated RNAs in archaebacteria /--by James W. Brown. (1988)
Advisor: John N. Reeve, MCD Biology Program.
Brown, James W., Thomm, Michael, Beckler, Gregory S., Frey, Gerhard, Stetter, Karl O., Reeve, John N.
Transcription initiation of the hisA sent in vico. in the archaebacterium Methanococcus vannielii, as determined by nuclease S1 and primer extension analyses, occurs 73 base pairs (bp) upstream of...
Pereira, Suzette L., Grayling, Rowan A., Lurz, Rudi, Reeve, John N.
Archaea contain histones that have primary sequences in common with eukaryal nucleosome core histones and a three-dimensional structure that is essentially only the histone fold. Here we report the...
Archaeal Nucleosome Positioning by CTG Repeats
Sandman, Kathleen, Reeve, John N.
DNA shape recognition determines the preferred binding sites for sequence-independent DNA binding proteins, and here we document that archaeal histones assemble archaeal nucleosomes in vitro centered...
Methanobacterium thermoautotrophicum RNA Polymerase and Transcription In Vitro
Darcy, Trevor J., Hausner, Winfried, Awery, Donald E., Edwards, Aled M., Thomm, Michael, Reeve, John N.
RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum ΔH has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or...
Li, Wen-Tyng, Shriver, John W., Reeve, John N.
Amino acid residues responsible for the large difference in thermostability between HMfB and HFoB, archaeal histones from the hyperthermophile Methanothermus fervidus and the mesophile...
Hanzelka, Brian L., Darcy, Trevor J., Reeve, John N.
In the archaeon Methanobacterium thermoautotrophicum, MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the α-subunits of eucaryal general transcription factor TFIIE....
The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity
Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...
Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...
Mucopeptide Biosynthesis by Minicells of Escherichia coli
Minicells produced by Escherichia coli χ925 incorporated amino acids and N-acetyl-d-glucosamine into mucopeptide.
Growth of the Bacillus subtilis Cell Surface
A double mutant strain, Bacillus subtilis div IV B1 dal trp, has been constructed which grows as filamentous cells and minicells and requires d-alanine for growth. Removal of d-alanine from a growing...
Physiological Studies of Bacillus subtilis Minicells
Mendelson, Neil H., Reeve, John N., Cole, Roger M.
Minicells produced by Bacillus subtilis strains carrying the div IV-B1 mutation, (CU 403 div IV-B1 and CU 403 div IV-B1, tag-1), were purified by a procedure which destroys parental cells with...
Reeve, John N., Clark, D. Joseph
When Escherichia coli BUG-6 is grown at 42 C and then returned to 30 C, the division kinetics during the recovery at 30 C are dependent on the length of time the cells were grown at 42 C. If...
Reeve, John N., Clark, D. Joseph
When Escherichia coli BUG-6 is shifted from 30 C to 36 or 38 C, division does not stop, but the rate of division of the cell population is initially decreased followed by a period of increased rate...
Reeve, John N., Groves, David J., Clark, D. Joseph
A temperature-sensitive division mutant of Escherichia coli was isolated by using differential filtration to select for filaments at 42 C and normal cells at 30 C. Cells shifted from 30 to 42 C stop...
Minicells of Bacillus subtilis
Reeve, John N., Mendelson, Neil H., Coyne, Sheila I., Hallock, Linda L.
After nitrosoguanidine (N-methyl-N′-nitro-N-nitrosoguanidine) mutagenesis, two Bacillus subtilis mutants (div IV-A1 and div IV-B1) were isolated that are defective in the location of division site...
Minicells of Bacillus subtilis: a New Bacteriophage-Blocking Agent
Reeve, John N., Mendelson, Neil H.
Bacteriophage SP01, SP17, and φ29 rapidly absorb irreversibly to minicells of Bacillus subtilis but do not produce a lytic cycle in minicells.
Transcription by an Archaeal RNA Polymerase Is Slowed but Not Blocked by an Archaeal Nucleosome
Archaeal RNA polymerases (RNAPs) are closely related to eukaryotic RNAPs, and in Euryarchaea, genomic DNA is wrapped and compacted by histones into archaeal nucleosomes. In eukaryotes, transcription...
Transcription initiation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding protein (TBP), transcription factor B (TFB), and RNA polymerase (RNAP). The...
Use of a restriction enzyme-digested PCR product as substrate for helicase assays
Shin, Jae-Ho, Reeve, John N., Kelman, Zvi
DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not...
Čuboňová, L'ubomíra, Sandman, Kathleen, Hallam, Steven J., DeLong, Edward F., Reeve, John N.
Archaeal histone-encoding genes have been identified in marine Crenarchaea. The protein encoded by a representative of these genes, synthesized in vitro and expressed in Escherichia coli, binds DNA...
Regulation of Tryptophan Operon Expression in the Archaeon Methanothermobacter thermautotrophicus
Conserved trp genes encode enzymes that catalyze tryptophan biosynthesis in all three biological domains, and studies of their expression in Bacteria and eukaryotes have revealed a variety of...
Soares, Divya J., Marc, Frédéric, Reeve, John N.
Although the archaeal and eukaryotic nucleosome core histones evolved from a common ancestor, conserved lysine residues are present at DNA-binding locations in all four eukaryotic histones that are...
Pereira, Suzette L., Grayling, Rowan A., Lurz, Rudi, Reeve, John N.
Archaea contain histones that have primary sequences in common with eukaryal nucleosome core histones and a three-dimensional structure that is essentially only the histone fold. Here we report the...
Archaeal Nucleosome Positioning by CTG Repeats
Sandman, Kathleen, Reeve, John N.
DNA shape recognition determines the preferred binding sites for sequence-independent DNA binding proteins, and here we document that archaeal histones assemble archaeal nucleosomes in vitro centered...
Methanobacterium thermoautotrophicum RNA Polymerase and Transcription In Vitro
Darcy, Trevor J., Hausner, Winfried, Awery, Donald E., Edwards, Aled M., Thomm, Michael, Reeve, John N.
RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum ΔH has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or...
Li, Wen-Tyng, Shriver, John W., Reeve, John N.
Amino acid residues responsible for the large difference in thermostability between HMfB and HFoB, archaeal histones from the hyperthermophile Methanothermus fervidus and the mesophile...
Hanzelka, Brian L., Darcy, Trevor J., Reeve, John N.
In the archaeon Methanobacterium thermoautotrophicum, MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the α-subunits of eucaryal general transcription factor TFIIE....
Soares, Divya J., Marc, Frédéric, Reeve, John N.
Although the archaeal and eukaryotic nucleosome core histones evolved from a common ancestor, conserved lysine residues are present at DNA-binding locations in all four eukaryotic histones that are...
The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity
Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...
Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...
Mucopeptide Biosynthesis by Minicells of Escherichia coli
Minicells produced by Escherichia coli χ925 incorporated amino acids and N-acetyl-d-glucosamine into mucopeptide.
Growth of the Bacillus subtilis Cell Surface
A double mutant strain, Bacillus subtilis div IV B1 dal trp, has been constructed which grows as filamentous cells and minicells and requires d-alanine for growth. Removal of d-alanine from a growing...
Physiological Studies of Bacillus subtilis Minicells
Mendelson, Neil H., Reeve, John N., Cole, Roger M.
Minicells produced by Bacillus subtilis strains carrying the div IV-B1 mutation, (CU 403 div IV-B1 and CU 403 div IV-B1, tag-1), were purified by a procedure which destroys parental cells with...
Reeve, John N., Clark, D. Joseph
When Escherichia coli BUG-6 is grown at 42 C and then returned to 30 C, the division kinetics during the recovery at 30 C are dependent on the length of time the cells were grown at 42 C. If...
Reeve, John N., Clark, D. Joseph
When Escherichia coli BUG-6 is shifted from 30 C to 36 or 38 C, division does not stop, but the rate of division of the cell population is initially decreased followed by a period of increased rate...
Reeve, John N., Groves, David J., Clark, D. Joseph
A temperature-sensitive division mutant of Escherichia coli was isolated by using differential filtration to select for filaments at 42 C and normal cells at 30 C. Cells shifted from 30 to 42 C stop...
Minicells of Bacillus subtilis
Reeve, John N., Mendelson, Neil H., Coyne, Sheila I., Hallock, Linda L.
After nitrosoguanidine (N-methyl-N′-nitro-N-nitrosoguanidine) mutagenesis, two Bacillus subtilis mutants (div IV-A1 and div IV-B1) were isolated that are defective in the location of division site...
Minicells of Bacillus subtilis: a New Bacteriophage-Blocking Agent
Reeve, John N., Mendelson, Neil H.
Bacteriophage SP01, SP17, and φ29 rapidly absorb irreversibly to minicells of Bacillus subtilis but do not produce a lytic cycle in minicells.
Transcription by an Archaeal RNA Polymerase Is Slowed but Not Blocked by an Archaeal Nucleosome
Archaeal RNA polymerases (RNAPs) are closely related to eukaryotic RNAPs, and in Euryarchaea, genomic DNA is wrapped and compacted by histones into archaeal nucleosomes. In eukaryotes, transcription...
Transcription initiation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding protein (TBP), transcription factor B (TFB), and RNA polymerase (RNAP). The...
Use of a restriction enzyme-digested PCR product as substrate for helicase assays
Shin, Jae-Ho, Reeve, John N., Kelman, Zvi
DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not...
Čuboňová, L'ubomíra, Sandman, Kathleen, Hallam, Steven J., DeLong, Edward F., Reeve, John N.
Archaeal histone-encoding genes have been identified in marine Crenarchaea. The protein encoded by a representative of these genes, synthesized in vitro and expressed in Escherichia coli, binds DNA...
Regulation of Tryptophan Operon Expression in the Archaeon Methanothermobacter thermautotrophicus
Conserved trp genes encode enzymes that catalyze tryptophan biosynthesis in all three biological domains, and studies of their expression in Bacteria and eukaryotes have revealed a variety of...
Spontaneous trpY Mutants and Mutational Analysis of the TrpY Archaeal Transcription Regulator▿
Čuboňová, L'ubomíra, Sandman, Kathleen, Karr, Elizabeth A., Cochran, Andrew J., Reeve, John N.
Over 90% of Methanothermobacter thermautotrophicus mutants isolated as spontaneously resistant to 5-methyl tryptophan had mutations in trpY. Most were single-base-pair substitutions that identified...
Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis▿
Santangelo, Thomas J., Čuboňová, L'ubomíra, Matsumi, Rie, Atomi, Haruyuki, Imanaka, Tadayuki, Reeve, John N.
An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense...
TrpY Regulation of trpB2 Transcription in Methanothermobacter thermautotrophicus▿
Karr, Elizabeth A., Sandman, Kathleen, Lurz, Rudi, Reeve, John N.
TrpY binds specifically to TRP box sequences upstream of trpB2, but the repression of trpB2 transcription requires additional TrpY assembly that is stimulated by but not dependent on the presence of...
Santangelo, Thomas J., Čuboňová, L'ubomíra, Reeve, John N.
Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was...
Nanoarchaeal Origin of Histone H3? ▿
Friedrich-Jahn, Ulrike, Aigner, Johanna, Längst, Gernot, Reeve, John N., Huber, Harald
NEQ288, one of two archaeal histones in Nanoarchaeum equitans, has a unique four-residue insertion that closely resembles an insertion in the eukaryotic histone H3 lineage. NEQ288 bound DNA but did...