Ciarlet, Max, Heiman, Erica, Delbeke, Thomas, McDonald, Sarah M, ...
Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11...
Matthijnssens, Jelle, Ciarlet, Max, Heiman, Erica, Arijs, Ingrid, Delbeke, Thomas, McDonald, Sarah M., ...
Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11...
Matthijnssens, Jelle, Ciarlet, Max, Heiman, Erica M., Arijs, Ingrid, Delbeke, Thomas, McDonald, Sarah M., ...
Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11...
Matthijnssens, Jelle, Ciarlet, Max, Heiman, Erica, Arijs, Ingrid, Delbeke, Thomas, McDonald, Sarah M., ...
Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11...
Ciarlet, Max, Attoui, Houssam, Bányai, Krisztián, Estes, Mary K, ...
Recently, a classification system was proposed for rotaviruses in which all the 11 genomic RNA segments are used (Matthijnssens et al. in J Virol 82:3204-3219, 2008). Based on nucleotide identity...
TORTORICI, M. ALEJANDRA, SHAPIRO, BRUCE A., PATTON, JOHN T.
Replication of the segmented double-stranded (ds)RNA genome of rotavirus requires the viral RNA-dependent RNA polymerase (RdRP) to use 11 different (+)RNAs as templates for (−) strand synthesis....
TORTORICI, M. ALEJANDRA, SHAPIRO, BRUCE A., PATTON, JOHN T.
Replication of the segmented double-stranded (ds)RNA genome of rotavirus requires the viral RNA-dependent RNA polymerase (RdRP) to use 11 different (+)RNAs as templates for (−) strand synthesis....
Lynn S. Silvestri, Zenobia F. Taraporewala, John T. Patton
Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified...
RNA-Binding and Capping Activities of Proteins in Rotavirus Open Cores
Guanylyltransferases are members of the nucleotidyltransferase family and function in mRNA capping by transferring GMP to the phosphate end of nascent RNAs. Although numerous guanylyltransferases...
Rotavirus RNA Replication Requires a Single-Stranded 3′ End for Efficient Minus-Strand Synthesis
The segmented double-stranded (ds) RNA genome of the rotaviruses is replicated asymmetrically, with viral mRNA serving as the template for the synthesis of minus-strand RNA. Previous studies with...
Taraporewala, Zenobia, Chen, Dayue, Patton, John T.
The nonstructural protein NSP2 is a component of rotavirus replication intermediates and accumulates in cytoplasmic inclusions (viroplasms), sites of genome RNA replication and the assembly of...
Taraporewala, Zenobia F., Patton, John T.
The rotavirus nonstructural protein NSP2 self-assembles into homomultimers, binds single-stranded RNA nonspecifically, possesses a Mg2+-dependent nucleoside triphosphatase (NTPase) activity, and is a...
Patton, John T., Taraporewala, Zenobia, Chen, Dayue, Chizhikov, Vladimir, Jones, Melinda, Elhelu, Azza, ...
The nonpolyadenylated mRNAs of rotavirus are templates for the synthesis of protein and the segmented double-stranded RNA (dsRNA) genome. During serial passage of simian SA11 rotaviruses in cell...
RNA-Binding Activity of the Rotavirus Phosphoprotein NSP5 Includes Affinity for Double-Stranded RNA
Vende, Patrice, Taraporewala, Zenobia F., Patton, John T.
Phosphoprotein NSP5 is a component of replication intermediates that catalyze the synthesis of the segmented double-stranded RNA (dsRNA) rotavirus genome. To study the role of the protein in viral...
Taraporewala, Zenobia F., Schuck, Peter, Ramig, Robert F., Silvestri, Lynn, Patton, John T.
Evidence that NSP2 plays a role in packaging and replication comes from studies on tsE(1400), a rotavirus mutant with a temperature-sensitive (ts) lesion in the NSP2 gene. Cells infected with tsE and...
Replication of Nondefective Parvoviruses: Lack of a Virion-Associated DNA Polymerase
Pritchard, Cynthia, Patton, John T., Bates, Robert C., Stout, Ernest R.
We have examined four of the nondefective parvoviruses for an associated DNA polymerase. Virions were purified from neuraminidase-treated infected-cell lysates by isopycnic centrifugation in CsCl or...
Rotavirus Replication: Plus-Sense Templates for Double-Stranded RNA Synthesis Are Made in Viroplasms
Silvestri, Lynn S., Taraporewala, Zenobia F., Patton, John T.
Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified...
Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein
Kearney, Karen, Chen, Dayue, Taraporewala, Zenobia F, Vende, Patrice, Hoshino, Yasutaka, Tortorici, Maria Alejandra, ...
Rotavirus, a cause of severe gastroenteritis, contains a segmented double-stranded (ds)RNA genome that replicates using viral mRNAs as templates. The highly conserved 3′-consensus sequence...
IFN regulatory factor 3 (IRF3), a constitutively expressed protein localizing largely to the cytoplasm, is a primary effector of the innate immune response. Infection can trigger the phosphorylation,...
Rotavirus Glycoprotein NSP4 Is a Modulator of Viral Transcription in the Infected Cell
Silvestri, Lynn S., Tortorici, M. Alejandra, Patton, John T.
The outer shell of the rotavirus triple-layered virion is lost during cell entry, yielding a double-layered particle (DLP) that directs synthesis of viral plus-strand RNAs. The plus-strand RNAs act...
TORTORICI, M. ALEJANDRA, SHAPIRO, BRUCE A., PATTON, JOHN T.
Replication of the segmented double-stranded (ds)RNA genome of rotavirus requires the viral RNA-dependent RNA polymerase (RdRP) to use 11 different (+)RNAs as templates for (−) strand synthesis....
Structure-Function Analysis of Rotavirus NSP2 Octamer by Using a Novel Complementation System†
Taraporewala, Zenobia F., Jiang, Xiaofang, Jayaram, Hariharan, Prasad, B. V. Venkataram, Patton, John T.
Viral inclusion bodies, or viroplasms, that form in rotavirus-infected cells direct replication and packaging of the segmented double-stranded RNA (dsRNA) genome. NSP2, one of two rotavirus proteins...
RNA-Binding and Capping Activities of Proteins in Rotavirus Open Cores
Guanylyltransferases are members of the nucleotidyltransferase family and function in mRNA capping by transferring GMP to the phosphate end of nascent RNAs. Although numerous guanylyltransferases...
Rotavirus RNA Replication Requires a Single-Stranded 3′ End for Efficient Minus-Strand Synthesis
The segmented double-stranded (ds) RNA genome of the rotaviruses is replicated asymmetrically, with viral mRNA serving as the template for the synthesis of minus-strand RNA. Previous studies with...
Taraporewala, Zenobia, Chen, Dayue, Patton, John T.
The nonstructural protein NSP2 is a component of rotavirus replication intermediates and accumulates in cytoplasmic inclusions (viroplasms), sites of genome RNA replication and the assembly of...
Taraporewala, Zenobia F., Patton, John T.
The rotavirus nonstructural protein NSP2 self-assembles into homomultimers, binds single-stranded RNA nonspecifically, possesses a Mg2+-dependent nucleoside triphosphatase (NTPase) activity, and is a...
Patton, John T., Taraporewala, Zenobia, Chen, Dayue, Chizhikov, Vladimir, Jones, Melinda, Elhelu, Azza, ...
The nonpolyadenylated mRNAs of rotavirus are templates for the synthesis of protein and the segmented double-stranded RNA (dsRNA) genome. During serial passage of simian SA11 rotaviruses in cell...
RNA-Binding Activity of the Rotavirus Phosphoprotein NSP5 Includes Affinity for Double-Stranded RNA
Vende, Patrice, Taraporewala, Zenobia F., Patton, John T.
Phosphoprotein NSP5 is a component of replication intermediates that catalyze the synthesis of the segmented double-stranded RNA (dsRNA) rotavirus genome. To study the role of the protein in viral...
Taraporewala, Zenobia F., Schuck, Peter, Ramig, Robert F., Silvestri, Lynn, Patton, John T.
Evidence that NSP2 plays a role in packaging and replication comes from studies on tsE(1400), a rotavirus mutant with a temperature-sensitive (ts) lesion in the NSP2 gene. Cells infected with tsE and...
Replication of Nondefective Parvoviruses: Lack of a Virion-Associated DNA Polymerase
Pritchard, Cynthia, Patton, John T., Bates, Robert C., Stout, Ernest R.
We have examined four of the nondefective parvoviruses for an associated DNA polymerase. Virions were purified from neuraminidase-treated infected-cell lysates by isopycnic centrifugation in CsCl or...
Rotavirus Replication: Plus-Sense Templates for Double-Stranded RNA Synthesis Are Made in Viroplasms
Silvestri, Lynn S., Taraporewala, Zenobia F., Patton, John T.
Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified...
Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein
Kearney, Karen, Chen, Dayue, Taraporewala, Zenobia F, Vende, Patrice, Hoshino, Yasutaka, Tortorici, Maria Alejandra, ...
Rotavirus, a cause of severe gastroenteritis, contains a segmented double-stranded (ds)RNA genome that replicates using viral mRNAs as templates. The highly conserved 3′-consensus sequence...
IFN regulatory factor 3 (IRF3), a constitutively expressed protein localizing largely to the cytoplasm, is a primary effector of the innate immune response. Infection can trigger the phosphorylation,...
Rotavirus Glycoprotein NSP4 Is a Modulator of Viral Transcription in the Infected Cell
Silvestri, Lynn S., Tortorici, M. Alejandra, Patton, John T.
The outer shell of the rotavirus triple-layered virion is lost during cell entry, yielding a double-layered particle (DLP) that directs synthesis of viral plus-strand RNAs. The plus-strand RNAs act...
TORTORICI, M. ALEJANDRA, SHAPIRO, BRUCE A., PATTON, JOHN T.
Replication of the segmented double-stranded (ds)RNA genome of rotavirus requires the viral RNA-dependent RNA polymerase (RdRP) to use 11 different (+)RNAs as templates for (−) strand synthesis....
Structure-Function Analysis of Rotavirus NSP2 Octamer by Using a Novel Complementation System†
Taraporewala, Zenobia F., Jiang, Xiaofang, Jayaram, Hariharan, Prasad, B. V. Venkataram, Patton, John T.
Viral inclusion bodies, or viroplasms, that form in rotavirus-infected cells direct replication and packaging of the segmented double-stranded RNA (dsRNA) genome. NSP2, one of two rotavirus proteins...
Secretion of interferon (IFN) by virus-infected cells is essential for activating autocrine and paracrine pathways that promote cellular transition to an antiviral state. In most mammalian cells, IFN...
Kumar, Mukesh, Jayaram, Hariharan, Jiang, Xiaofang, Taraporewala, Zenobia F., Jacobson, Raymond H., ...
Rotavirus, the major pathogen of infantile gastroenteritis, carries a nonstructural protein, NSP2, essential for viroplasm formation and genome replication/packaging. In addition to RNA-binding and...
McDonald, Sarah M., Patton, John T.
Group A rotaviruses are classified into serotypes, based on the reactivity pattern of neutralizing antibodies to VP4 and VP7, as well as into subgroups (SGs), based on non-neutralizing antibodies...
Matthijnssens, Jelle, Ciarlet, Max, Heiman, Erica, Arijs, Ingrid, Delbeke, Thomas, McDonald, Sarah M., ...
Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11...
Heiman, Erica M., McDonald, Sarah M., Barro, Mario, Taraporewala, Zenobia F., Bar-Magen, Tamara, Patton, John T.
Group A human rotaviruses (HRVs) are the major cause of severe viral gastroenteritis in infants and young children. To gain insight into the level of genetic variation among HRVs, we determined the...
McDonald, Sarah M., Matthijnssens, Jelle, McAllen, John K., Hine, Erin, Overton, Larry, Wang, Shiliang, ...
Group A human rotaviruses (RVs) are a major cause of severe gastroenteritis in infants and young children. Yet, aside from the genes encoding serotype antigens (VP7; G-type and VP4; P-type), little...