Jagu, Subhashini, Karanam, Balasubramanyam, Gambhira, Ratish, Chivukula, Sudha V., Chaganti, Revathi J., Lowy, Douglas R., ...
Background Vaccination with minor capsid protein L2 induces antibodies that cross-neutralize diverse papillomavirus types. However, neutralizing antibody titers against the papillomavirus type from...
Ji, Xuemei, Neumann, Ana S., Sturgis, Erich M., Adler-Storthz, Karen, Dahlstrom, Kristina R., Schiller, John T., ...
The tumor suppressor p53 protein can be bound, degraded and inactivated by the human papillomavirus (HPV) E6 oncoprotein. The p53 protein's susceptibility to this oncoprotein may be influenced by the...
Carrageenan Is a Potent Inhibitor of Papillomavirus Infection (2006)
Christopher B. Buck, Cynthia D. Thompson, Jeffrey N. Roberts, Martin Müller, Douglas R. Lowy, John T. Schiller
Certain sexually transmitted human papillomavirus (HPV) types are causally associated with the development of cervical cancer. Our recent development of high-titer HPV pseudoviruses has made it...
HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood (2005)
Pinto, Ligia A., Castle, Philip E., Roden, Richard B., Harro, Clayton D., Lowy, Douglas R., Schiller, John T., ...
Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n = 20) or placebo (n = 4) before...
HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood (2005)
Pinto, Ligia A., Castle, Philip E., Roden, Richard B., Harro, Clayton D., Lowy, Douglas R., Schiller, John T., ...
Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n = 20) or placebo (n = 4) before...
A population-based study of cervical carcinoma and HPV infection in Latvia. (2004)
Silins, Ilvars, Wang, Xiaohong, Tadesse, Amha, Jansen, Kathrin U, Schiller, John T, Avall-Lundqvist, Elisabeth, ...
Abstract is not available
Nardelli-Haefliger, Denise, Wirthner, Daniel, Schiller, John T., Lowy, Douglas R., Hildesheim, Allan, Ponci, Françoise, ...
Background: In early-phase trials, a human papillomavirus 16 (HPV16) virus–like particle (VLP) vaccine has been shown to be well tolerated, immunogenic, and protective against HPV16 in women, most...
Wong, May, Pagano, Joseph S., Schiller, John T., Tevethia, Satvir S., Raab-Traub, Nancy, Gruber, Jack
Harro, Clayton D., Pang, Yuk-Ying Susana, Roden, Richard B. S., Hildesheim, Allan, Wang, Zhaohui, Reynolds, Mary Jane, ...
Background: Studies in animal models have shown that systemic immunization with a papillomavirus virus-like particle (VLP) vaccine composed of L1, a major structural viral protein, can confer...
Papillomavirus-Like Particle Vaccines (2000)
Schiller, John T., Lowy, Douglas R.
Papillomavirus-like particle (VLP)-based subunit vaccines have undergone rapid development over the past 8 years. Three types are being investigated. The most basic type is composed of only the L1...
Lehtinen, Matti, Dillner, Joakim, Knekt, Paul, Luostarinen, Tapio, Aromaa, Arpo, Kirnbauer, Reinhard, ...
Br Med J 1996; 312: 537-539.
Han, Chenglong, Qiao, Guibin, Hubbert, Nancy L., Li, Liangshou, Sun, Changsheng, Wang, Yan, ...
Background: The existence of large geographic variations in the prevalence of esophageal cancer in some countries, such as China, indicates that environmental risk factors may be important in the...
Heino, Pirkko, Eklund, Carina, Fredriksson-Shanazarian, Vanoohi, Goldman, Sven, Schiller, John T., Dillner, Joakim
Background: Anal epidermoid carcinoma is a relatively rare tumor, but its incidence has been increasing rapidly during the past few years. Genetic material from the major oncogenic types of human...
Kirnbauer, Reinhard, Hubbert, Nancy L., Wheeler, Cosette M., Becker, Thomas M., Lowy, Douglas R., Schiller, John T.
Background Previous studies have demonstrated that genital infection with high-risk types of human papillomavirus (HPV), most often HPV16, is the most significant risk factor for the development of...
Greenstone, Heather L., Nieland, John D., De Visser, Karin E., Kirnbauer, Reinhard, Roden, Richard B. S., ...
Papillomavirus-like particles (VLPs) are a promising prophylactic vaccine candidate to prevent human papillomavirus (HPV) infections and associated epithelial neoplasia. However, they are unlikely to...
Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune...
Day, Patricia M., Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that...
L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation
Okun, Martin M., Day, Patricia M., Greenstone, Heather L., Booy, Frank P., Lowy, Douglas R., Schiller, John T., ...
BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid proteins L1 and L2 of BPV1, but...
Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection
Roden, Richard B. S., Day, Patricia M., Bronzo, Brian K., Yutzy, William H., Yang, Yanqin, Lowy, Douglas R., ...
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral...
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
High avidity and long-lasting autoantibodies to a self-polypeptide (TNF-α) were generated after parenteral vaccination of mice with low doses of virus-like particle–based (VLP-based) vaccines that...
Efficient Intracellular Assembly of Papillomaviral Vectors
Buck, Christopher B., Pastrana, Diana V., Lowy, Douglas R., Schiller, John T.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of...
Chackerian, Bryce, Briglio, Lindsey, Albert, Paul S., Lowy, Douglas R., Schiller, John T.
Antibodies against CCR5, the major coreceptor for human immunodeficiency virus type 1 (HIV-1), may have antiviral potential as viral fusion inhibitors. In this study, we generated a virus-like...
Day, Patricia M., Baker, Carl C., Lowy, Douglas R., Schiller, John T.
Previous studies have suggested that most papillomaviruses enter the host cell via clathrin-dependent receptor-mediated endocytosis but have not addressed later steps in viral entry. To examine these...
Maturation of Papillomavirus Capsids
Buck, Christopher B., Thompson, Cynthia D., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The papillomavirus capsid is a nonenveloped icosahedral shell formed by the viral major structural protein, L1. It is known that disulfide bonds between neighboring L1 molecules help to stabilize the...
Kämper, Nadine, Day, Patricia M., Nowak, Thorsten, Selinka, Hans-Christoph, Florin, Luise, Bolscher, Jan, ...
Papillomaviruses are internalized via clathrin-dependent endocytosis. However, the mechanism by which viral genomes pass endosomal membranes has not been elucidated. In this report we show that the...
Prophylactic human papillomavirus vaccines
Lowy, Douglas R., Schiller, John T.
Human papillomavirus (HPV) infection causes virtually all cases of cervical cancer, the second most common cause of death from cancer among women worldwide. This Review examines prophylactic HPV...
Carrageenan Is a Potent Inhibitor of Papillomavirus Infection
Buck, Christopher B, Thompson, Cynthia D, Roberts, Jeffrey N, Müller, Martin, Lowy, Douglas R, Schiller, John T
Certain sexually transmitted human papillomavirus (HPV) types are causally associated with the development of cervical cancer. Our recent development of high-titer HPV pseudoviruses has made it...
Human α-defensins block papillomavirus infection
Buck, Christopher B., Day, Patricia M., Thompson, Cynthia D., Lubkowski, Jacek, Lu, Wuyuan, Lowy, Douglas R., ...
Sexually transmitted human papillomaviruses (HPVs) are the primary cause of cervical cancer. Recent advances in techniques for production of papillomaviral vectors [known as pseudoviruses (PsVs)]...
Richards, Rebecca M., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
Papillomaviruses (PV) comprise a large family of nonenveloped DNA viruses that include the oncogenic PV types that are the causative agents of human cervical cancer. As is true of many animal DNA...
Greenstone, Heather L., Nieland, John D., De Visser, Karin E., Kirnbauer, Reinhard, Roden, Richard B. S., ...
Papillomavirus-like particles (VLPs) are a promising prophylactic vaccine candidate to prevent human papillomavirus (HPV) infections and associated epithelial neoplasia. However, they are unlikely to...
Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune...
Day, Patricia M., Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that...
L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation
Okun, Martin M., Day, Patricia M., Greenstone, Heather L., Booy, Frank P., Lowy, Douglas R., Schiller, John T., ...
BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid proteins L1 and L2 of BPV1, but...
Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection
Roden, Richard B. S., Day, Patricia M., Bronzo, Brian K., Yutzy, William H., Yang, Yanqin, Lowy, Douglas R., ...
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral...
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
High avidity and long-lasting autoantibodies to a self-polypeptide (TNF-α) were generated after parenteral vaccination of mice with low doses of virus-like particle–based (VLP-based) vaccines that...
Efficient Intracellular Assembly of Papillomaviral Vectors
Buck, Christopher B., Pastrana, Diana V., Lowy, Douglas R., Schiller, John T.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of...
Chackerian, Bryce, Briglio, Lindsey, Albert, Paul S., Lowy, Douglas R., Schiller, John T.
Antibodies against CCR5, the major coreceptor for human immunodeficiency virus type 1 (HIV-1), may have antiviral potential as viral fusion inhibitors. In this study, we generated a virus-like...
Day, Patricia M., Baker, Carl C., Lowy, Douglas R., Schiller, John T.
Previous studies have suggested that most papillomaviruses enter the host cell via clathrin-dependent receptor-mediated endocytosis but have not addressed later steps in viral entry. To examine these...
Maturation of Papillomavirus Capsids
Buck, Christopher B., Thompson, Cynthia D., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The papillomavirus capsid is a nonenveloped icosahedral shell formed by the viral major structural protein, L1. It is known that disulfide bonds between neighboring L1 molecules help to stabilize the...
Kämper, Nadine, Day, Patricia M., Nowak, Thorsten, Selinka, Hans-Christoph, Florin, Luise, Bolscher, Jan, ...
Papillomaviruses are internalized via clathrin-dependent endocytosis. However, the mechanism by which viral genomes pass endosomal membranes has not been elucidated. In this report we show that the...
Human α-defensins block papillomavirus infection
Buck, Christopher B., Day, Patricia M., Thompson, Cynthia D., Lubkowski, Jacek, Lu, Wuyuan, Lowy, Douglas R., ...
Sexually transmitted human papillomaviruses (HPVs) are the primary cause of cervical cancer. Recent advances in techniques for production of papillomaviral vectors [known as pseudoviruses (PsVs)]...
Richards, Rebecca M., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
Papillomaviruses (PV) comprise a large family of nonenveloped DNA viruses that include the oncogenic PV types that are the causative agents of human cervical cancer. As is true of many animal DNA...
Prophylactic human papillomavirus vaccines
Lowy, Douglas R., Schiller, John T.
Human papillomavirus (HPV) infection causes virtually all cases of cervical cancer, the second most common cause of death from cancer among women worldwide. This Review examines prophylactic HPV...
Carrageenan Is a Potent Inhibitor of Papillomavirus Infection
Buck, Christopher B, Thompson, Cynthia D, Roberts, Jeffrey N, Müller, Martin, Lowy, Douglas R, Schiller, John T
Certain sexually transmitted human papillomavirus (HPV) types are causally associated with the development of cervical cancer. Our recent development of high-titer HPV pseudoviruses has made it...
Day, Patricia M., Thompson, Cynthia D., Buck, Christopher B., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The mechanisms of human papillomavirus (HPV) neutralization by antibodies are incompletely understood. We have used HPV16 pseudovirus infection of HaCaT cells to analyze how several neutralizing...
García-Piñeres, Alfonso, Hildesheim, Allan, Dodd, Lori, Kemp, Troy J., Williams, Marcus, Harro, Clayton, ...
To determine the systemic cytokine pattern induced by vaccination with human papillomavirus (HPV) L1 virus-like particles (VLP), we analyzed 22 different cytokines in culture supernatants of L1...
Kemp, Troy J., Hildesheim, Allan, Falk, Roni T., Schiller, John T., Lowy, Douglas R., Rodriguez, Ana Cecilia, ...
Immunogenicity evaluations in human papillomavirus (HPV) vaccine trials have relied on serological samples, yet cervical antibodies are likely to be most relevant for protection against infection. In...
Day, Patricia M., Gambhira, Ratish, Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
Pseudovirions of human papillomavirus type 16 (HPV16), the principal etiologic agent in 50% of cervical cancers, were used as a model system to investigate the cell surface interactions involved in...
Human Papillomavirus Type 16 Entry: Retrograde Cell Surface Transport along Actin-Rich Protrusions
Schelhaas, Mario, Ewers, Helge, Rajamäki, Minna-Liisa, Day, Patricia M., Schiller, John T., Helenius, Ari
The lateral mobility of individual, incoming human papillomavirus type 16 pseudoviruses (PsV) bound to live HeLa cells was studied by single particle tracking using fluorescence video microscopy. The...
Arrangement of L2 within the Papillomavirus Capsid▿
Buck, Christopher B., Cheng, Naiqian, Thompson, Cynthia D., Lowy, Douglas R., Steven, Alasdair C., Schiller, John T., ...
Papillomaviruses are a family of nonenveloped DNA tumor viruses. Some sexually transmitted human papillomavirus (HPV) types, including HPV type 16 (HPV16), cause cancer of the uterine cervix....
Alphs, Hannah H., Gambhira, Ratish, Karanam, Balasubramanyam, Roberts, Jeffrey N., Jagu, Subhashini, Schiller, John T., ...
Persistent infection with the high-risk subset of genitotropic human papillomavirus (HPV) genotypes is a necessary cause of cervical cancer. Given the global burden of cervical cancer, a low-cost,...
Heparan Sulfate-Independent Cell Binding and Infection with Furin-Precleaved Papillomavirus Capsids▿
Day, Patricia M., Lowy, Douglas R., Schiller, John T.
Papillomavirus infection normally involves virion binding to cell surface heparan sulfate proteoglycans (HSPGs). However, we found that human papillomavirus type 16 pseudovirions efficiently bound...
Jagu, Subhashini, Karanam, Balasubramanyam, Gambhira, Ratish, Chivukula, Sudha V., Chaganti, Revathi J., Lowy, Douglas R., ...
Johnson, Katherine M., Kines, Rhonda C., Roberts, Jeffrey N., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
The host factors required for in vivo infection have not been investigated for any papillomavirus. Using a recently developed murine cervicovaginal challenge model, we evaluated the importance of...