John Wain

Molecular Typing of Multiple-Antibiotic-Resistant Salmonella enterica Serovar Typhi from Vietnam: Application to Acute and Relapse Cases of Typhoid Fever

Wain, John, Hien, Tran T., Connerton, Phillippa, Ali, Tahir, M. Parry, Christopher, Chinh, Nguyen T. T., ...

The rate of multiple-antibiotic resistance is increasing among Salmonella enterica serovar Typhi strains in Southeast Asia. Pulsed-field gel electrophoresis (PFGE) and other typing methods were used...

Value of a Single-Tube Widal Test in Diagnosis of Typhoid Fever in Vietnam

Parry, Christopher M., Hoa, Nguyen Thi Tuyet, Diep, To Song, Wain, John, Chinh, Nguyen Tran, Vinh, Ha, ...

The diagnostic value of an acute-phase single-tube Widal test for suspected typhoid fever was evaluated with 2,000 Vietnamese patients admitted to an infectious disease referral hospital between 1993...

Epidemic Typhoid in Vietnam: Molecular Typing of Multiple-Antibiotic-Resistant Salmonella enterica Serotype Typhi from Four Outbreaks

Connerton, Phillippa, Wain, John, Hien, Tran T., Ali, Tahir, Parry, Christopher, Chinh, Nguyen T., ...

Multidrug-resistant Salmonella enterica serotype Typhi isolates from four outbreaks of typhoid fever in southern Vietnam between 1993 and 1997 were compared. Pulsed-field gel electrophoresis,...

Serology of Typhoid Fever in an Area of Endemicity and Its Relevance to Diagnosis

House, Deborah, Wain, John, Ho, Vo A., Diep, To S., Chinh, Nguyen T., Bay, Phan V., ...

Currently, the laboratory diagnosis of typhoid fever is dependent upon either the isolation of Salmonella enterica subsp. enterica serotype Typhi from a clinical sample or the detection of raised...

Quantitation of Bacteria in Bone Marrow from Patients with Typhoid Fever: Relationship between Counts and Clinical Features

Wain, John, Bay, Phan Van Be, Vinh, Ha, Duong, Nguyen M., Diep, To Song, Walsh, Amanda L., ...

Enteric fever is the only bacterial infection of humans for which bone marrow examination is routinely recommended. A prospective study of the concentrations of bacteria in the bone marrow and their...

Nasal Carriage in Vietnamese Children of Streptococcus pneumoniae Resistant to Multiple Antimicrobial Agents

Parry, Christopher M., Diep, To Song, Wain, John, Hoa, Nguyen Thi Tuyet, Gainsborough, Mary, Nga, Diem, ...

Resistance to antimicrobial agents in Streptococcus pneumoniae is increasing rapidly in many Asian countries. There is little recent information concerning resistance levels in Vietnam. A prospective...

A Randomized Controlled Comparison of Azithromycin and Ofloxacin for Treatment of Multidrug-Resistant or Nalidixic Acid-Resistant Enteric Fever

Chinh, Nguyen Tran, Parry, Christopher M., Ly, Nguyen Thi, Ha, Huynh Duy, Thong, Mai Xuan, Diep, To Song, ...

To examine the efficacy and safety of short courses of azithromycin and ofloxacin for treating multidrug-resistant (MDR, i.e., resistant to chloramphenicol, ampicillin, and cotrimoxazole) and...

Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid

Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., ...

Population genetic studies suggest that Yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from Yersinia pseudotuberculosis. Plasmid acquisition is likely to have...

Quantitation of Bacteria in Blood of Typhoid Fever Patients and Relationship between Counts and Clinical Features, Transmissibility, and Antibiotic Resistance

Wain, John, Diep, To Song, Ho, Vo Anh, Walsh, Amanda M., Hoa, Nguyen Thi Tuyet, Parry, Christopher M., ...

Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood,...

Emergence in Vietnam of Streptococcus pneumoniae Resistant to Multiple Antimicrobial Agents as a Result of Dissemination of the Multiresistant Spain23F-1 Clone

Parry, Christopher M., Duong, Nguyen Minh, Zhou, Jiaji, Mai, Nguyen Thi Hoang, Diep, To Song, Thinh, Le Quoc, ...

Surveillance for Streptococcus pneumoniae resistant to penicillin and other antimicrobial agents is necessary to define the optimal empirical antibiotic therapy for meningitis in resource-poor...

Composition, Acquisition, and Distribution of the Vi Exopolysaccharide-Encoding Salmonella enterica Pathogenicity Island SPI-7

Pickard, Derek, Wain, John, Baker, Stephen, Line, Alexandra, Chohan, Sonia, Fookes, Maria, ...

Vi capsular polysaccharide production is encoded by the viaB locus, which has a limited distribution in Salmonella enterica serovars. In S. enterica serovar Typhi, viaB is encoded on a 134-kb...

Molecular Analysis of incHI1 Antimicrobial Resistance Plasmids from Salmonella Serovar Typhi Strains Associated with Typhoid Fever

Wain, John, Diem Nga, L. T., Kidgell, Claire, James, Keith, Fortune, Sarah, Song Diep, To, ...

The first outbreak of multidrug-resistant (MDR) typhoid fever in Vietnam was in 1993, and by 1995 nearly 90% of cases were MDR. Plasmid HCM1, sequenced in full, is an incHI1 plasmid from Salmonella...

Vi Antigen Expression in Salmonella enterica Serovar Typhi Clinical Isolates from Pakistan

Wain, John, House, Deborah, Zafar, Afia, Baker, Stephen, Nair, Satheesh, Kidgell, Claire, ...

The accurate identification of Salmonella enterica subsp. enterica serovar Typhi variants that fail to express the capsular polysaccharide, Vi, is an important and much discussed issue for medical...

Use of Paired Serum Samples for Serodiagnosis of Typhoid Fever

House, Deborah, Chinh, Nguyen T., Diep, To S., Parry, Christopher M., Wain, John, Dougan, Gordon, ...

Using an enzyme-linked immunosorbent assay we demonstrate that, in adult patients with typhoid fever, the sensitivity of a serological test based on the detection of anti-lipopolysaccharide...

Detection of Vi-Negative Salmonella enterica Serovar Typhi in the Peripheral Blood of Patients with Typhoid Fever in the Faisalabad Region of Pakistan

Baker, Stephen, Sarwar, Yasra, Aziz, Hafsa, Haque, Asma, Ali, Aamir, Dougan, Gordon, ...

The synthesis and transportation proteins of the Vi capsular polysaccharide of Salmonella enterica serovar Typhi (serovar Typhi) are encoded by the viaB operon, which resides on a 134-kb...

Molecular Typing of Multiple-Antibiotic-Resistant Salmonella enterica Serovar Typhi from Vietnam: Application to Acute and Relapse Cases of Typhoid Fever

Wain, John, Hien, Tran T., Connerton, Phillippa, Ali, Tahir, M. Parry, Christopher, Chinh, Nguyen T. T., ...

The rate of multiple-antibiotic resistance is increasing among Salmonella enterica serovar Typhi strains in Southeast Asia. Pulsed-field gel electrophoresis (PFGE) and other typing methods were used...

Value of a Single-Tube Widal Test in Diagnosis of Typhoid Fever in Vietnam

Parry, Christopher M., Hoa, Nguyen Thi Tuyet, Diep, To Song, Wain, John, Chinh, Nguyen Tran, Vinh, Ha, ...

The diagnostic value of an acute-phase single-tube Widal test for suspected typhoid fever was evaluated with 2,000 Vietnamese patients admitted to an infectious disease referral hospital between 1993...

Epidemic Typhoid in Vietnam: Molecular Typing of Multiple-Antibiotic-Resistant Salmonella enterica Serotype Typhi from Four Outbreaks

Connerton, Phillippa, Wain, John, Hien, Tran T., Ali, Tahir, Parry, Christopher, Chinh, Nguyen T., ...

Multidrug-resistant Salmonella enterica serotype Typhi isolates from four outbreaks of typhoid fever in southern Vietnam between 1993 and 1997 were compared. Pulsed-field gel electrophoresis,...

Serology of Typhoid Fever in an Area of Endemicity and Its Relevance to Diagnosis

House, Deborah, Wain, John, Ho, Vo A., Diep, To S., Chinh, Nguyen T., Bay, Phan V., ...

Currently, the laboratory diagnosis of typhoid fever is dependent upon either the isolation of Salmonella enterica subsp. enterica serotype Typhi from a clinical sample or the detection of raised...

Quantitation of Bacteria in Bone Marrow from Patients with Typhoid Fever: Relationship between Counts and Clinical Features

Wain, John, Bay, Phan Van Be, Vinh, Ha, Duong, Nguyen M., Diep, To Song, Walsh, Amanda L., ...

Enteric fever is the only bacterial infection of humans for which bone marrow examination is routinely recommended. A prospective study of the concentrations of bacteria in the bone marrow and their...

Nasal Carriage in Vietnamese Children of Streptococcus pneumoniae Resistant to Multiple Antimicrobial Agents

Parry, Christopher M., Diep, To Song, Wain, John, Hoa, Nguyen Thi Tuyet, Gainsborough, Mary, Nga, Diem, ...

Resistance to antimicrobial agents in Streptococcus pneumoniae is increasing rapidly in many Asian countries. There is little recent information concerning resistance levels in Vietnam. A prospective...

A Randomized Controlled Comparison of Azithromycin and Ofloxacin for Treatment of Multidrug-Resistant or Nalidixic Acid-Resistant Enteric Fever

Chinh, Nguyen Tran, Parry, Christopher M., Ly, Nguyen Thi, Ha, Huynh Duy, Thong, Mai Xuan, Diep, To Song, ...

To examine the efficacy and safety of short courses of azithromycin and ofloxacin for treating multidrug-resistant (MDR, i.e., resistant to chloramphenicol, ampicillin, and cotrimoxazole) and...

Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid

Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., ...

Population genetic studies suggest that Yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from Yersinia pseudotuberculosis. Plasmid acquisition is likely to have...

Quantitation of Bacteria in Blood of Typhoid Fever Patients and Relationship between Counts and Clinical Features, Transmissibility, and Antibiotic Resistance

Wain, John, Diep, To Song, Ho, Vo Anh, Walsh, Amanda M., Hoa, Nguyen Thi Tuyet, Parry, Christopher M., ...

Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood,...

Emergence in Vietnam of Streptococcus pneumoniae Resistant to Multiple Antimicrobial Agents as a Result of Dissemination of the Multiresistant Spain23F-1 Clone

Parry, Christopher M., Duong, Nguyen Minh, Zhou, Jiaji, Mai, Nguyen Thi Hoang, Diep, To Song, Thinh, Le Quoc, ...

Surveillance for Streptococcus pneumoniae resistant to penicillin and other antimicrobial agents is necessary to define the optimal empirical antibiotic therapy for meningitis in resource-poor...

Composition, Acquisition, and Distribution of the Vi Exopolysaccharide-Encoding Salmonella enterica Pathogenicity Island SPI-7

Pickard, Derek, Wain, John, Baker, Stephen, Line, Alexandra, Chohan, Sonia, Fookes, Maria, ...

Vi capsular polysaccharide production is encoded by the viaB locus, which has a limited distribution in Salmonella enterica serovars. In S. enterica serovar Typhi, viaB is encoded on a 134-kb...

Molecular Analysis of incHI1 Antimicrobial Resistance Plasmids from Salmonella Serovar Typhi Strains Associated with Typhoid Fever

Wain, John, Diem Nga, L. T., Kidgell, Claire, James, Keith, Fortune, Sarah, Song Diep, To, ...

The first outbreak of multidrug-resistant (MDR) typhoid fever in Vietnam was in 1993, and by 1995 nearly 90% of cases were MDR. Plasmid HCM1, sequenced in full, is an incHI1 plasmid from Salmonella...

Vi Antigen Expression in Salmonella enterica Serovar Typhi Clinical Isolates from Pakistan

Wain, John, House, Deborah, Zafar, Afia, Baker, Stephen, Nair, Satheesh, Kidgell, Claire, ...

The accurate identification of Salmonella enterica subsp. enterica serovar Typhi variants that fail to express the capsular polysaccharide, Vi, is an important and much discussed issue for medical...

Use of Paired Serum Samples for Serodiagnosis of Typhoid Fever

House, Deborah, Chinh, Nguyen T., Diep, To S., Parry, Christopher M., Wain, John, Dougan, Gordon, ...

Using an enzyme-linked immunosorbent assay we demonstrate that, in adult patients with typhoid fever, the sensitivity of a serological test based on the detection of anti-lipopolysaccharide...

Detection of Vi-Negative Salmonella enterica Serovar Typhi in the Peripheral Blood of Patients with Typhoid Fever in the Faisalabad Region of Pakistan

Baker, Stephen, Sarwar, Yasra, Aziz, Hafsa, Haque, Asma, Ali, Aamir, Dougan, Gordon, ...

The synthesis and transportation proteins of the Vi capsular polysaccharide of Salmonella enterica serovar Typhi (serovar Typhi) are encoded by the viaB operon, which resides on a 134-kb...

Randomized Controlled Comparison of Ofloxacin, Azithromycin, and an Ofloxacin-Azithromycin Combination for Treatment of Multidrug-Resistant and Nalidixic Acid-Resistant Typhoid Fever▿

Parry, Christopher M., Ho, Vo Anh, Phuong, Le Thi, Bay, Phan Van Be, Lanh, Mai Ngoc, Tung, Le Thanh, ...

Isolates of Salmonella enterica serovar Typhi that are multidrug resistant (MDR, resistant to chloramphenicol, ampicillin, and trimethoprim-sulfamethoxazole) and have reduced susceptibility to...

Multidrug-Resistant Salmonella enterica Serovar Paratyphi A Harbors IncHI1 Plasmids Similar to Those Found in Serovar Typhi▿

Holt, Kathryn E., Thomson, Nicholas R., Wain, John, Phan, Minh Duy, Nair, Satheesh, Hasan, Rumina, ...

Salmonella enterica serovars Typhi and Paratyphi A cause systemic infections in humans which are referred to as enteric fever. Multidrug-resistant (MDR) serovar Typhi isolates emerged in the 1980s,...

Prophage Sequences Defining Hot Spots of Genome Variation in Salmonella enterica Serovar Typhimurium Can Be Used To Discriminate between Field Isolates▿

Cooke, Fiona J., Wain, John, Fookes, Maria, Ivens, Alasdair, Thomson, Nicholas, Brown, Derek J., ...

Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by...

Antimicrobial Drug Resistance of Salmonella enterica Serovar Typhi in Asia and Molecular Mechanism of Reduced Susceptibility to the Fluoroquinolones▿

Chau, Tran Thuy, Campbell, James Ian, Galindo, Claudia M., Van Minh Hoang, Nguyen, Diep, To Song, Nga, Tran Thu Thi, ...

This study describes the pattern and extent of drug resistance in 1,774 strains of Salmonella enterica serovar Typhi isolated across Asia between 1993 and 2005 and characterizes the molecular...

Molecular Characterization of the Salmonella enterica Serovar Typhi Vi-Typing Bacteriophage E1▿

Pickard, Derek, Thomson, Nicholas R., Baker, Stephen, Wain, John, Pardo, Mercedes, Goulding, David, ...

Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi...