José Moniz-Pereira

Modification of the mycobacteriophage Ms6 attPcore allows the integration of multiple vectors into different tRNAalaT-loops in slow- and fast-growing mycobacteria (2006)

Dos Vultos, Tiago, Méderlé, Isabelle, Abadie, Valérie, Pimentel, Madalena, Moniz-Pereira, José, Gicquel, Brigitte, ...

Abstract Background Mycobacteriophage Ms6 integrates into Mycobacterium smegmatis and M. bovis BCG chromosome at the 3' end of tRNA ala genes. Homologous recombination occurs between the phage attP...

Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNAala T-loops in slow- and fast-growing mycobacteria. (2006)

Vultos, Tiago Dos, Méderlé, Isabelle, Abadie, Valérie, Pimentel, Madalena, Moniz-Pereira, José, Gicquel, Brigitte, ...

BACKGROUND: Mycobacteriophage Ms6 integrates into Mycobacterium smegmatis and M. bovis BCG chromosome at the 3' end of tRNAala genes. Homologous recombination occurs between the phage attP core and...

Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNAala T-loops in slow- and fast-growing mycobacteria. (2006)

Vultos, Tiago Dos, Méderlé, Isabelle, Abadie, Valérie, Pimentel, Madalena, Moniz-Pereira, José, Gicquel, Brigitte, ...

BACKGROUND: Mycobacteriophage Ms6 integrates into Mycobacterium smegmatis and M. bovis BCG chromosome at the 3' end of tRNAala genes. Homologous recombination occurs between the phage attP core and...

Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNAala T-loops in slow- and fast-growing mycobacteria. (2006)

Vultos, Tiago Dos, Méderlé, Isabelle, Abadie, Valérie, Pimentel, Madalena, Moniz-Pereira, José, Gicquel, Brigitte, ...

BACKGROUND: Mycobacteriophage Ms6 integrates into Mycobacterium smegmatis and M. bovis BCG chromosome at the 3' end of tRNAala genes. Homologous recombination occurs between the phage attP core and...

Discrimination of Multidrug-Resistant Mycobacterium tuberculosis IS6110 Fingerprint Subclusters by rpoB Gene Mutation Analysis

Portugal, Isabel, Maia, Sara, Moniz-Pereira, José

The rpoB gene mutations in a 69-bp region of the gene, resulting in resistance to rifampin, were used to discriminate between Mycobacterium tuberculosis IS6110 fingerprint subclusters. These...

Primary Human Immunodeficiency Virus Type 2 (HIV-2) Isolates Infect CD4-Negative Cells via CCR5 and CXCR4: Comparison with HIV-1 and Simian Immunodeficiency Virus and Relevance to Cell Tropism In Vivo

Reeves, Jacqueline D., Hibbitts, Sam, Simmons, Graham, McKnight, Áine, Azevedo-Pereira, José M., Moniz-Pereira, José, ...

Cell surface receptors exploited by human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) for infection are major determinants of tropism. HIV-1 usually requires two receptors to...

Virological and Molecular Demonstration of Human Immunodeficiency Virus Type 2 Vertical Transmission

Cavaco-Silva, Patricia, Taveira, Nuno C., Rosado, Lino, Lourenço, Maria H., Moniz-Pereira, José, Douglas, Nigel W., ...

To demonstrate that human immunodeficiency virus type 2 (HIV-2) mother-to-child transmission exists, HIV-2 isolates were obtained from both an asymptomatic mother (HIV-2 strain ARM), and her child...

Expression of Mycobacteriophage Ms6 Lysis Genes Is Driven by Two σ70-Like Promoters and Is Dependent on a Transcription Termination Signal Present in the Leader RNA

Garcia, Miguel, Pimentel, Madalena, Moniz-Pereira, José

A mycobacteriophage Ms6 strong promoter region (Plys) was isolated by using transcriptional fusions with the lacZ reporter gene. Two tandem σ70-like promoter sequences (P1 and P2) were found in this...

pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Isolates in Portugal

Portugal, Isabel, Barreiro, Luís, Moniz-Pereira, José, Brum, Laura

The nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were...

Functional Analysis of Vif Protein Shows Less Restriction of Human Immunodeficiency Virus Type 2 by APOBEC3G

Ribeiro, Ana Clara, Maia E Silva, Alexandra, Santa-Marta, Mariana, Pombo, Ana, Moniz-Pereira, José, Goncalves, Joao, ...

Viral infectivity factor (Vif) is one of the human immunodeficiency virus (HIV) accessory proteins and is conserved in the primate lentivirus group. This protein is essential for viral replication in...

Discrimination of Multidrug-Resistant Mycobacterium tuberculosis IS6110 Fingerprint Subclusters by rpoB Gene Mutation Analysis

Portugal, Isabel, Maia, Sara, Moniz-Pereira, José

The rpoB gene mutations in a 69-bp region of the gene, resulting in resistance to rifampin, were used to discriminate between Mycobacterium tuberculosis IS6110 fingerprint subclusters. These...

Primary Human Immunodeficiency Virus Type 2 (HIV-2) Isolates Infect CD4-Negative Cells via CCR5 and CXCR4: Comparison with HIV-1 and Simian Immunodeficiency Virus and Relevance to Cell Tropism In Vivo

Reeves, Jacqueline D., Hibbitts, Sam, Simmons, Graham, McKnight, Áine, Azevedo-Pereira, José M., Moniz-Pereira, José, ...

Cell surface receptors exploited by human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) for infection are major determinants of tropism. HIV-1 usually requires two receptors to...

Virological and Molecular Demonstration of Human Immunodeficiency Virus Type 2 Vertical Transmission

Cavaco-Silva, Patricia, Taveira, Nuno C., Rosado, Lino, Lourenço, Maria H., Moniz-Pereira, José, Douglas, Nigel W., ...

To demonstrate that human immunodeficiency virus type 2 (HIV-2) mother-to-child transmission exists, HIV-2 isolates were obtained from both an asymptomatic mother (HIV-2 strain ARM), and her child...

Expression of Mycobacteriophage Ms6 Lysis Genes Is Driven by Two σ70-Like Promoters and Is Dependent on a Transcription Termination Signal Present in the Leader RNA

Garcia, Miguel, Pimentel, Madalena, Moniz-Pereira, José

A mycobacteriophage Ms6 strong promoter region (Plys) was isolated by using transcriptional fusions with the lacZ reporter gene. Two tandem σ70-like promoter sequences (P1 and P2) were found in this...

pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Isolates in Portugal

Portugal, Isabel, Barreiro, Luís, Moniz-Pereira, José, Brum, Laura

The nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were...

Functional Analysis of Vif Protein Shows Less Restriction of Human Immunodeficiency Virus Type 2 by APOBEC3G

Ribeiro, Ana Clara, Maia E Silva, Alexandra, Santa-Marta, Mariana, Pombo, Ana, Moniz-Pereira, José, Goncalves, Joao, ...

Viral infectivity factor (Vif) is one of the human immunodeficiency virus (HIV) accessory proteins and is conserved in the primate lentivirus group. This protein is essential for viral replication in...