Joseph A. Krzycki

Clustered Genes Encoding the Methyltransferases of Methanogenesis from Monomethylamine

Burke, Stephen A., Lo, Sam L., Krzycki, Joseph A.

Coenzyme M (CoM) is methylated during methanogenesis from monomethyamine in a reaction catalyzed by three proteins. Using monomethylamine, a 52-kDa polypeptide termed monomethylamine...

The Trimethylamine Methyltransferase Gene and Multiple Dimethylamine Methyltransferase Genes of Methanosarcina barkeri Contain In-Frame and Read-Through Amber Codons†

Paul, Ligi, Ferguson, Donald J., Krzycki, Joseph A.

Three different methyltransferases initiate methanogenesis from trimethylamine (TMA), dimethylamine (DMA) or monomethylamine (MMA) by methylating different cognate corrinoid proteins that are...

The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity

Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...

Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...

Clustered Genes Encoding the Methyltransferases of Methanogenesis from Monomethylamine

Burke, Stephen A., Lo, Sam L., Krzycki, Joseph A.

Coenzyme M (CoM) is methylated during methanogenesis from monomethyamine in a reaction catalyzed by three proteins. Using monomethylamine, a 52-kDa polypeptide termed monomethylamine...

The Trimethylamine Methyltransferase Gene and Multiple Dimethylamine Methyltransferase Genes of Methanosarcina barkeri Contain In-Frame and Read-Through Amber Codons†

Paul, Ligi, Ferguson, Donald J., Krzycki, Joseph A.

Three different methyltransferases initiate methanogenesis from trimethylamine (TMA), dimethylamine (DMA) or monomethylamine (MMA) by methylating different cognate corrinoid proteins that are...

The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity

Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...

Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...

A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine

Longstaff, David G., Larue, Ross C., Faust, Joseph E., Mahapatra, Anirban, Zhang, Liwen, Green-Church, Kari B., ...

Pyrrolysine has entered natural genetic codes by the translation of UAG, a canonical stop codon. UAG translation as pyrrolysine requires the pylT gene product, an amber-decoding tRNAPyl that is...

Structure of the α2ε2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarbonylase/synthase complex

Gong, Weimin, Hao, Bing, Wei, Zhiyi, Ferguson, Donald J., Tallant, Thomas, Krzycki, Joseph A., ...

Ni-dependent carbon monoxide dehydrogenases (Ni-CODHs) are a diverse family of enzymes that catalyze reversible CO:CO2 oxidoreductase activity in acetogens, methanogens, and some CO-using bacteria....