Kinet, Virginie, Nguyen, Ngoc-Quynh-Nhu, Sabatel, Céline, Blacher, Silvia, Noël, Agnès, Martial, Joseph, ...
Human 16K PRL (16K hPRL) is a potent inhibitor of angiogenesis both in vitro and in vivo. It has been shown to prevent tumor growth in three xenograft mouse models. Here we have used a gene transfer...
Plasminogen activator inhibitor-1 protects endothelial cells from FasL-mediated apoptosis. (2008)
Bajou, Khalid, Peng, H, Laug, WE, Maillard, Catherine, Noël, Agnès, Foidart, Jean-Michel, ...
Plasminogen activator inhibitor-1 (PAI-1) paradoxically enhances tumor progression and angiogenesis; however, the mechanism supporting this role is not known. Here we provide evidence that PAI-1 is...
Zebrafish Sox7 and Sox18 function together to control arterial-venous identity (2008)
Pendeville-Samain, Hélène, Winandy, Marie, Manfroid, Isabelle, Nivelles, Olivier, Motte, Patrick, Pasque, V, ...
Sox7 and Sox18 are members of the F-subgroup of Sox transcription factors family and are mostly expressed in endothelial compartments. In humans, dominant mutations in Sox18 are the underlying cause...
Delporte, F M, Pasque, Vincent, Devos, Nathalie, Manfroid, Isabelle, Voz, Marianne, Motte, Patrick, ...
BACKGROUND: PAX6 is a transcription factor playing a crucial role in the development of the eye and in the differentiation of the pancreatic endocrine cells as well as of enteroendocrine cells....
Nguyen, Ngoc-Quynh-Nhu, Cornet, Anne, Blacher, Silvia, Tabruyn, Sébastien, Foidart, Jean-Michel, Noël, Agnès, ...
Tumor metastases, the most fearsome aspect of cancer, are generally resistant to conventional therapies. Angiogenesis is a crucial aspect of tumor growth and metastatic dissemination. Antiangiogenic...
Erdmann, S, Ricken, A, Merkwitz, C, Struman, Ingrid, Castino, R, Hummitzsch, K, ...
In the corpus luteum (CL), blood vessels develop, stabilize, and regress. This process depends on the ratio of pro-and antiangiogenic factors, which change during the ovarian cycle. The present study...
Evrard, Christine, Maes, D, Zegers, I, Declercq, JP, Vanhee, C, Martial, Joseph, ...
The capillary counterdiffusion method is a very efficient crystallization technique for obtaining high-quality protein crystals. This technique requires a convection-free environment, which can be...
Muller, Marc, Mavropoulos, A, Nica, G, Motte, Patrick, Martial, Joseph
16/06/2007
Tabruyn, SP, Sabatel, Céline, Nguyen, Ngoc-Quynh-Nhu, Verhaeghe, C, Castermans, Karolien, Malvaux, Ludovic, ...
The 16-kDa N-terminal fragment of human prolactin (16K hPRL) is a potent angiostatic factor that inhibits tumor growth in mouse models. Using microarray experiments, we have dissected how the...
Tabruyn, Sébastien, Sabatel, Céline, Nguyen, Ngoc-Quynh-Nhu, Castermans, Karolien, Malvaux, Ludovic, ...
The 16-kDa N-terminal fragment of human prolactin (16K hPRL) is a potent angiostatic factor that inhibits tumor growth in mouse models. Using microarray experiments, we have dissected how the...
Manfroid, Isabelle, Delporte, F, Baudhuin, A, Motte, P, Neumann, C J, Voz, Marianne, ...
In amniotes, the pancreatic mesenchyme plays a crucial role in pancreatic epithelium growth, notably through the secretion of fibroblast growth factors. However, the factors involved in the formation...
Nguyen, Ngoc-Quynh-Nhu, Tabruyn, Sebastien, Lins, Laurence, Lion, Michelle, Cornet, AM, Lair, Florence, ...
Angiogenesis is a crucial step in many pathologies, including tumor growth and metastasis. Here, we show that tilted peptides exert antiangiogenic activity. Tilted (or oblique-oriented) peptides are...
Expression of the somatolactin beta gene during zebrafish embryonic development (2006)
Lopez, Mauricio, Nica, G, Motte, Patrick, Martial, Joseph, Hammerschmidt, Matthias, Muller, Marc
Somatolactin (Sl) is a pituitary hormone closely related to prolactin (Prl) and growth hormone that was until now only found in various fish species. We isolated the cDNA coding for zebrafish Sl beta...
Expression and functional properties of four slow skeletal troponin T isoforms in rat muscles (2005)
Kischel, Philippe, Bastide, Bruno, Muller, Marc, Dubail, Fabien, Offredi, Fabrice, Jin, Jian-Ping, ...
We investigated the expression and functional properties of slow skeletal troponin T(sTnT) isoforms in rat skeletal muscles. Four sTnT cDNAs were cloned from the slow soleus muscle. Three isoforms...
Tabruyn, Sébastien, Nguyen, Ngoc-Quynh-Nhu, Cornet, Anne, Martial, Joseph, Struman, Ingrid
The 16-kDa N-terminal fragment of human prolactin (16K hPRL) is a potent antiangiogenic factor that has been shown to prevent tumor growth in a xenograph mouse model. In this paper we first...
Willemsen, Philippe, Scippo, Marie-Louise, Maghuin-Rogister, Guy, Martial, Joseph, Muller, Marc
We have previously generated several transformed human mammary cell lines for the detection of steroid receptor-mediated activities and used these cell lines to detect and characterize steroid...
Solution structure of human prolactin (2005)
Teilum, K, Hoch, J C, Goffin, Vincent, Kinet, Sandrina, Martial, Joseph, Kragelund, B B
We report the solution structure of human prolactin determined by NMR spectroscopy. Our result is a significant improvement over a previous structure in terms of number and distribution of distance...
sox4b is a key player of pancreatic alpha cell differentiation in zebrafish (2005)
Mavropoulos, A, Devos, Nathalie, Biemar, Frédéric, Zecchin, Elisabetta, Argenton, Francesco, Edlund, Helena, ...
Pancreas development relies on a network of transcription factors belonging mainly to the Homeodomain and basic Helix-Loop-Helix families. We show in this study that, in zebrafish, sox4, a member of...
Evidence for a role of prolactin fragments in corpus luteum function (2005)
Ricken, A, Merkwitz, C, Struman, Ingrid, Martial, Joseph, Spanel-Borowski, K
Piwnica, D, Touraine, P, Struman, Ingrid, Tabruyn, S, Bolbach, G, Clapp, C, ...
16K prolactin (PRL) is the name given to the 16-kDa N-terminal fragment obtained by proteolysis of rat PRL by tissue extracts or cell lysates, in which cathepsin D was identified as the candidate...
Pan, H, Nguyen, Ngoc-Quynh-Nhu, Yoshida, H, Bentzien, F, Shaw, LC, Rentier-Delrue, Françoise, ...
PURPOSE. To examine the ability and mechanism of the 16 kDa N-terminal fragment of human prolactin (16K hPRL) in the inhibition of abnormal retinal neovascularization. METHODS. The 16K hPRL-encoding...
Receptor-based screening assays: New perspectives in anti-doping control (2004)
Scippo, Marie-Louise, Willemsen, Philippe, Danyi, Sophie, Helbo, Vincent, Muller, Marc, Martial, Joseph, ...
The so-called "growth promoters", steroid hormones and beta-agonists, are currently controlled by using hyphenated analytical methods (chromatography coupled to mass spectrometry) or, sometimes for...
Scippo, Marie-Louise, Argiris, Catherine, Van De Weerdt, Cécile, Muller, Marc, Willemsen, Philippe, Martial, Joseph, ...
This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERalpha, hPR and hAR). The tested chemicals are organochlorine...
Use of reporter cell lines for detection of endocrine-disrupter activity (2004)
Willemsen, Philippe, Scippo, Marie-Louise, Kausel, G, Figueroa, J, Maghuin-Rogister, Guy, Martial, Joseph, ...
We have studied stable transformed human mammary cell lines with highly inducible steroid receptor-mediated luciferase reporter gene expression. Cells responding specifically to glucocorticoids,...
Tabruyn, Sébastien, Sorlet, CM, Rentier-Delrue, Françoise, Bours, Vincent, Weiner, RI, Martial, Joseph, ...
We have previously shown that the 16-kDa N-terminal fragment of human prolactin (16K hPRL) has antiangiogenic properties, including the ability to induce apoptosis in vascular endothelial cells....
Offredi, Fabrice, Dubail, Fabien, Kischel, Philippe, Sarinski, K, Stern, AS, Van De Weerdt, Cécile, ...
We have designed, synthesized, and characterized a 216 amino acid residue sequence encoding a putative idealized alpha/beta-barrel protein. The design was elaborated in two steps. First, the...
Development of pure prolactin receptor antagonists (2003)
Bernichtein, Sophie, Kayser, Christine, Dillner, Karin, Moulin, Stéphanie, Kopchick, John J., Martial, Joseph, ...
Prolactin (PRL) promotes tumor growth in various experimental models and leads to prostate hyperplasia and mammary neoplasia in PRL transgenic mice. Increasing experimental evidence argues for the...
Transcription of the human prolactin gene in mammary cells (2002)
Baudhuin, A, Manfroid, Isabelle, Van De Weerdt, Cécile, Martial, Joseph, Muller, Marc
Expression of human prolactin in the Mammary gland, one of the main target organs of this hormone, leads to the formation of an autocrine-paracrine proliferative loop in this tissue. Involvement of...
Detection of illegal growth promoters in biological samples using receptor binding assays (2002)
Scippo, Marie-Louise, Van De Weerdt, Cécile, Willemsen, Philippe, François, Jean-Marie, Rentier-Delrue, Françoise, Muller, Marc, ...
In the European Union (EU), the use of growth-promoting substances in meat production is banned. The control of growth promoters, especially steroid hormones, is presently based on expensive and...
Willemsen, Philippe, Scippo, Marie-Louise, Maghuin-Rogister, Guy, Martial, Joseph, Muller, Marc
We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection...
Expression of the zinc finger Egr1 gene during zebrafish embryonic development (2002)
Close, Renaud, Toro, Sabrina, Martial, Joseph, Muller, Marc
Egr1 is a highly conserved zinc finger protein which plays important roles in many aspects of vertebrate development and in the adult. The cDNA coding for zebrafish Egr1 was obtained and its...
Molina, A, Carpeaux, Rudy, Martial, Joseph, Muller, Marc
We obtained a stable transformed fish (EPC) cell line containing a reporter gene under the control of the tilapia HSP70 promoter. Expression of the reporter gene, coding for a green fluorescent...
Differential expression of two somatostatin genes during zebrafish embryonic development (2002)
Devos, Nathalie, Deflorian, Gianluca, Biemar, Frédéric, Bortolussi, Marino, Martial, Joseph, Peers, Bernard, ...
We have identified the cDNAs of two new zebrafish preprosomatostatins, PPSS1 and PPSS3, in addition to the previously cloned PPSS2 (Argenton et al., 1999). PPSS1 is the orthologue of mammalian PPSSs,...
Bentzien, F, Struman, Ingrid, Martini, J F, Martial, Joseph, Weiner, R
The M(r) 16,000 NH(2)-terminal fragment of human prolactin (16K hPRL) is a potent antiangiogenic factor inhibiting endothelial cell function in vitro and neovascularization in vivo. The present study...
S179D-human PRL, a pseudophosphorylated human PRL analog, is an agonist and not an antagonist (2001)
Bernichtein, Sophie, Kinet, Sandrina, Jeay, Sébastien, Llovera, Marta, Madern, Dominique, Martial, Joseph, ...
For many years, our group has been involved in the development of human PRL antagonists. In two recent publications, S179D-human PRL, a human PRL analog designed to mimic a putative...
Molina, Alfredo, Di Martino, Emmanuel, Martial, Joseph, Muller, Marc
We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell...
Molina, Alfredo, Iyengar, Arati, Marins, Luis F., Biemar, Frédéric, Hanley, Sean, Maclean, Norman, ...
We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also...
Nishimura, Tadahiro, Narita, Tadashi, Miyazaki, Emi, Ito, Tohru, Nishimoto, Norihiro, Yoshizaki, Kazuyuki, ...
Expression of the human low-affinity Fc receptors for IgG (human Fc gamma RII) is differentially regulated. We report here the characterization of the promoter structure of the human Fc gamma RIIB...
Biemar, Frédéric, Devos, Nathalie, Martial, Joseph, Driever, Wolfgang, Peers, Bernard
Meis and Prep/Pknox (MEINOX family) proteins, together with Pbx (PBC family) proteins, belong to the TALE superfamily characterized by an atypical homeodomain containing three additional amino acids...
KINET, Sandrina, BERNICHTEIN, Sophie, LLOVERA, Marta, KELLY, Paul A., Martial, Joseph, GOFFIN, Vincent
In this review, we make a chronologically overview of a project aimed at deciphering the molecular bases of the mechanism by which prolactin, a pituitary-secreted polypeptidic hormone, interacts with...
Pendeville-Samain, Hélène, Carpino, Nick, Marine, Jean-Christophe, Takahashi, Yukata, Muller, Marc, Martial, Joseph, ...
Overexpression and inhibitor studies have suggested that the c-Myc target gene for ornithine decarboxylase (ODC), the enzyme which converts ornithine to putrescine, plays an important role in diverse...
Orosz, F, Olah, J, Alvarez, M, Keseru, G M, Szabo, B, Wagner, G, ...
In a Hungarian family with severe decrease in triosephosphate isomerase (TPI) activity, 2 germ line-identical but phenotypically differing compound heterozygote brothers inherited 2 independent...
Martini, J F, Piot, C, Humeau, L M, Struman, Ingrid, Martial, Joseph, Weiner, R I
We asked whether the antiangiogenic action of 16K human PRL (hPRL), in addition to blocking mitogen-induced vascular endothelial cell proliferation, involved activation of programmed cell death....
Cloning and expression analysis of an inducible HSP70 gene from tilapia fish (2000)
Molina, Alfredo, Biemar, Frédéric, Muller, Ferenc, Iyengar, Arati, Prunet, Patrick, Maclean, Norman, ...
We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock...
Far upstream sequences regulate the human prolactin promoter transcription (2000)
Van De Weerdt, Cécile, Peers, Bernard, Belayew, A, Martial, Joseph, Muller, Marc
The human prolactin gene is mainly expressed in pituitary lactotrope cells, but transcription from an alternative, far upstream promoter was detected in lymphoid, placental and mammary cells. We...
Kausel, Gudrun, Vera, María Inés, San Martin, Rody, Figueroa, Jaime, Molina, Alfredo, Muller, Marc, ...
A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined,...
Struman, Ingrid, Bentzien, F, Lee, H, Mainfroid, V, D'Angelo, G, Goffin, Vincent, ...
Angiogenesis, the process of development of a new microvasculature, is regulated by a balance of positive and negative factors. We show both in vivo and in vitro that the members of the human...
Jacquemin, Patrick, Depetris, Danielle, Mattei, Marie-Geneviève, Martial, Joseph, Davidson, Irwin
Embryonic TEA domain-containing factor (ETF) belongs to the family of proteins structurally related to transcriptional enhancer factor-1 (TEF-1) and is implicated in neural development. Isolation and...
Maes, Dominique, Zeelen, Johan P., Thanki, Narmada, Beaucamp, Nicola, Alvarez, Marco, Thi, Minh Hoa Dao, ...
The molecular mechanisms that evolution has been employing to adapt to environmental temperatures are poorly understood. To gain some further insight into this subject we solved the crystal structure...
Sekkali, Belaid, Brim, Hassan, Muller, Marc, Argenton, Francesco, Bortolussi, Marino, Colombo, Lorenzo, ...
A gene encoding the Tilapia mossambica (Oreochromis mossambicus) growth hormone (tiGH) was isolated and sequenced. The gene spans 5.6 kb, including 3.7 kb of 5' and 0.2 kb of 3' flanking sequences...
Goudet, G, Delhalle, S, Biemar, F, Martial, Joseph, Peers, Bernard
Expression of the somatostatin gene in endocrine pancreatic cells is controlled by several regulatory cis-elements located in the promoter region. Among these, the adjacent UE-A and TSEI elements,...
Lee, H, Struman, Ingrid, Clapp, C, Martial, Joseph, Weiner, R I
The N-terminal fragment of PRL (16K PRL) is an antiangiogenic factor that, in vitro, inhibits several components of angiogenesis including basic fibroblast growth factor (bFGF)-induced cell division,...
Enhanced expression in seminoma of human zinc finger genes located on chromosome 19 (1998)
Ogawa, Takehiko, Poncelet, Dominique A, Kinoshita, Yuzo, Noce, Toshiaki, Takeda, Mitsumasa, Kawamoto, Kanji, ...
Six Kruppel-type zinc finger (ZF) genes were cloned from a seminoma cDNA library. One, ZFS-1, showed high sequence homology to the ZNF91 KRAB (Kruppel-associated box) ZF gene family and also the same...
Pernasetti, Flavia, Milner, Robert DG, De Zegher, Francis, Chavez, Viviana M, Muller, Marc, ...
Pit-1, a member of the POU-homeo domain protein family, is one of the transcription factors responsible for anterior pituitary development and pituitary-specific gene expression. Here, we describe...
Poncelet, D A, Bellefroid, E J, Bastiaens, P V, Demoitie, M A, Marine, J C, Pendeville-Samain, Hélène, ...
We previously identified the ZNF85 (HPF4) KRAB zinc finger gene, a member of the human ZNF91 family. Here, we show that the ZNF85 gene is highly expressed in normal adult testis, in seminomas, and in...
Ward, L, Chavez, M, Huot, C, Lecocq, P, Collu, R, Decarie, J C, ...
A 20-month-old boy presented with severe congenital growth hormone, thyrotropin, and prolactin deficiencies resulting from a de novo mutation of the PIT-1 gene. This form of congenital...
Régulation transcriptionnelle du gène de la prolactine humaine (1998)
Muller, Marc, BERWAER, M, CACCAVELLI, L, Manfroid, Isabelle, NALDA, A, Pendeville-Samain, Hélène, ...
Le gène humain de la prolactine (hPRL) est exprimé essentiellement par l'antéhypophyse. L'analyse des éléments régulateurs de la transcription sur plus de 5 000 bases en amont du site de début...
Effect of seasonal acclimatization on the expression of the carp transcription factor Pit-1 (1998)
Kausel, G, Vera, MI, Figueroa, J, Hernández, J, San Martin, R, Molina, A, ...
We isolated a clone comprising four exons of the carp Pit-1 gene. Using synthetic oligonucleotide probes derived from the carp Pit-1 sequence Pit-1 expression was assessed by in situ hybridization in...
Oury, Cécile, Alsat, E, Jacquemin, P, Evain-Brion, D, Martial, Joseph, Muller, Marc
Human chorionic somatomammotropin (hCS) is encoded by two highly related genes, hCS-A and hCS-B, located in the hGH/hCS gene locus. Both genes are expressed in the syncytiotrophoblast layer of the...
Jacquemin, P, Alsat, E, Oury, Cécile, Belayew, A, Muller, Marc, Evain-Brion, D, ...
The hCS-A and hCS-B genes encoding human chorionic somatomammotropin and the related hCS-L gene are very similar in their coding and flanking sequences. For each of these genes, downstream enhancers,...
Mainfroid, Véronique, Mande, Shekhar C, Hol, Wim G J, Martial, Joseph, Goraj, Karine
Human triosephosphate isomerase (hTIM) is a dimeric enzyme of identical subunits, adopting the alpha/beta-barrel fold. In a previous work, a monomeric mutant of hTIM was engineered in which Met14 and...
Poncelet, AC, Levavi-Sivan, B, Muller, Marc, Yaron, Z, Martial, Joseph, Belayew, A
To study the elements involved in the pituitary specific transcriptional regulation of the tilapia prolactin I gene (tiPRL I), we have cloned and entirely sequenced a 3.4-kb genomic fragment...
Regulation of prolactin gene expression in fishes (1996)
PONCELET, AC, YARON, Z, LEVAVI-SIVAN, B, Martial, Joseph, Muller, Marc
D'Angelo, G, Struman, Ingrid, Martial, Joseph, Weiner, R I
A number of factors both stimulating and inhibiting angiogenesis have been described. In the current work, we demonstrate that the angiogenic factor vascular endothelial growth factor (VEGF)...
Auperin, B, Rentier-Delrue, Françoise, Martial, Joseph, Prunet, P
Prolactin (PRL) receptors in gill tissue have been analyzed in tilapia (Oreochromis niloticus) after transfer from fresh water (FW) to brackish water (BW). This study has indicated the presence of...
The prenatal role of thyroid hormone evidenced by fetomaternal Pit-1 deficiency (1995)
De Zegher, F, Pernasetti, F, Vanhole, C, Devlieger, H, Martial, Joseph
The role of thyroid hormone in the human fetus is uncertain; a significant amount of T4 is transferred from the maternal to the fetal circulation. A mother-infant pair was found to be heterozygotic...
Delboni, Luis F, Mande, Shekhar C, Rentier-Delrue, Françoise, Mainfroid, Véronique, Turley, Stewart, Vellieux, Frederique M D, ...
The structure of the thermostable triosephosphate isomerase (TIM) from Bacillus stearothermophilus complexed with the competitive inhibitor 2-phosphoglycolate was determined by X-ray crystallography...
Use of a model to understand prolactin and growth hormone specificities (1995)
Goffin, Vincent, Martial, Joseph, Summers, Neena L.
A human prolactin (hPRL) model based on a 2.1 A resolution X-ray refinement of porcine growth hormone is reported. Only regions clearly defined in the growth hormone template (the four-helix bundle)...
Houbrechts, Annick, Moreau, Benoît, Abagyan, Ruben, Mainfroid, Véronique, Preaux, Gisèle, Lamproye, Alain, ...
The sequence of octarellin I, the first de novo (beta/alpha)8 polypeptide, was revised according to several criteria, among others the symmetry of the sequence, beta-residue volume and...
Wera, S, Zheng, L, Hooghe-Peters, E L, Belayew, A, Martial, Joseph, Velkeniers, B
It is at present well established that prolactin exerts a non-specific immunoactivating function. In this work we tested whether the immunosuppressant drugs cyclosporin A, FK506 and rapamycin...
Cellular and molecular aspects of thymic T-cell education to neurohypophysial peptides (1995)
Geenen, Vincent, Vandersmissen, Eric, Martens, Henri, Goxe, Béatrice, Kecha, Ouafae, Legros, Jean-Jacques, ...
Our studies have shown that oxytocin (OT) is the dominant peptide of the neurohypophysial (NHP) family that is expressed by thymic epithelial/nurse cells (TEC/TNC). Both in specific RIA and ICC...
Cellular and molecular aspects of thymic T-cell education to neurohypophysial peptides (1995)
Geenen, Vincent, Vandersmissen, Eric, Martens, Henri, Goxe, Béatrice, Kecha, Ouafae, Legros, Jean-Jacques, ...
Our studies have shown that oxytocin (OT) is the dominant peptide of the neurohypophysial (NHP) family that is expressed by thymic epithelial/nurse cells (TEC/TNC). Both in specific RIA and ICC...
Cellular and molecular aspects of thymic T-cell education to neurohypophysial peptides (1995)
Geenen, Vincent, Martens, Henri, Vandersmissen, Eric, Kecha, Ouafae, Benhida, Abdellah, Legros, Jean-Jacques, ...
Evidence for a Second Receptor Binding Site on Human Prolactin (1994)
Goffin, Vincent, Struman, Ingrid, Mainfroid, V, Kinet, Sandrina, Martial, Joseph
The existence of a second receptor binding site on human prolactin (hPRL) was investigated by site-directed mutagenesis. First, 12 residues of helices 1 and 3 were mutated to alanine. Since none of...
Geenen, Vincent, Cormann-Goffin, Nadine, Vandersmissen, Eric, Martens, Henri, Benhida, Abdellah, Martial, Joseph, ...
A comparative study of reporter gene activities in fish cells and embryos (1994)
Belayew, Alexandra, Martial, Joseph
Transcriptional regulation of gene expression is often studied by fusing the DNA sequences of interest (and their mutants) with a reporter gene. The resulting hybrid gene is introduced into cells...
Auperin, B, Rentier-Delrue, Françoise, Martial, Joseph, Prunet, P
In tilapia, there are two forms of prolactin (PRL) whose effects on sodium and chloride movements differ and depend on the living environment of the fish. To see whether different receptors or the...
Auperin, B, Rentier-Delrue, Françoise, Martial, Joseph, Prunet, P
Two forms of prolactin (tiPRLI and tiPRLII), with only 69% sequence identity, have been previously described in the cichlid fish tilapia (Oreochromis species). In the present study we have attempted...
Berwaer, M, Martial, Joseph, Davis, J R
The human PRL gene is expressed outside pituitary lactotrophs in decidualized endometrium and lymphoid cells, but here the mRNA contains a 5'-untranslated region from an additional noncoding exon 1a....
Jacquemin, P, Oury, Cécile, Belayew, A, Martial, Joseph
The transcriptional activity of the human placental lactogen genes (choriosomatomammotropic hormone, hCS) is controlled by tissue-specific enhancers located 4 kb downstream from their respective...
Kishan, Radha, Zeelen, Ph. Johan, Noble, Martin E.M., Borchert, Torben V., Mainfroid, Véronique, Goraj, Karine, ...
The crystal structure of a hybrid Escherichia coli triosephosphate isomerase (TIM) has been determined at 2.8 A resolution. The hybrid TIM (ETIM8CHI) was constructed by replacing the eighth beta...
Mande, S C, Mainfroid, V, Kalk, K H, Goraj, K, Martial, Joseph, Hol, W G
The crystal structure of recombinant human triosephosphate isomerase (hTIM) has been determined complexed with the transition-state analogue 2-phosphoglycolate at a resolution of 2.8 A. After...
Assignment of the human ZNF83 (HPF1) zinc finger gene to chromosome 19q13.3-q13.4 (1994)
Marine, Jean-Christophe, Bellefroid, Eric J., Bourguignon, Catherine, Riviere, Michèle, Lecoq, Pierre J., Poncelet, Dominique A., ...
Crystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide (1994)
Merritt, E A, Sarfaty, S, L'Hoir, C, Martial, Joseph, Hol, W G
Cholera toxin (CT) is an AB5 hexameric protein responsible for the symptoms produced by Vibrio cholerae infection. In the first step of cell intoxication, the B-pentamer of the toxin binds...
Lecocq-Xhonneux, F, Thiry, M, Dheur, I, Rossius, M, Vanderheijden, N, Martial, Joseph, ...
Viral haemorrhagic septicaemia (VHS) is a fish rhabdovirus infection of world-wide importance. Control policies have been established but the disease still causes heavy losses in fish farming. The...
A comparative study of reporter gene activities in fish cells and embryos (1994)
Sekkali, B, Hellemans, B, Belayew, A, Martial, Joseph, Ollivier, F, Volckaert, FA
Geenen, Vincent, Vandersmissen, E, Cormann-Goffin, N, Martens, Henri, Legros, Jean-Jacques, Degiovanni, G, ...
Thymic epithelial and nurse cells (TEC/TNC) synthesize an oxytocin (OT)-like peptide in association with a neurophysin (NP)-related protein in a way similar to in the hypothalamo-neurohypophysial...
Geenen, Vincent, Martens, Henri, Robert, Françoise, Benhida, Abdellah, Cormann-Goffin, Nadine, Legros, Jean-Jacques, ...
Goffin, Vincent, Struman, Ingrid, Goormaghtigh, E, Martial, Joseph
We have added nine extra residues to the C-terminal of human prolactin and analysed the effect of this mutation on the ability of the hormone to bind to its lactogenic receptor and to induce Nb2 cell...
Thymic Neurohypophysial-Related Peptides and T Cell Selection (1993)
Geenen, Vincent, Cormann-Goffin, N, Martens, Henri, Vandersmissen, E, Robert, F, Benhida, A, ...
Berwaer, Monique, Peers, Bernard, Nalda, Asuncion M, Monget, Philippe, Davis, Julian R, Belayew, Alexandra, ...
Pituitary GH3 cells were transfected with different deletion mutants of the human prolactin (hPRL) promoter fused to the CAT reporter gene. The proximal region (-250 to -42) was sufficient to confer...
Efficient lipofection of human trophoblast cells in primary cultures (1993)
Jacquemin, P, Alsat, E, Oury, Cécile, Belayew, A, Martial, Joseph, Evain-Brion, D
Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this...
Mainfroid, Véronique, Goraj, Karine, Rentier-Delrue, Françoise, Houbrechts, Annick, Loiseau, A, Gohimont, A C, ...
In order to investigate how structural modifications interfere with protein stability, we modified a (beta alpha)-unit in E.coli triosephosphate isomerase (TIM), a typical (beta alpha)-barrel...
Structure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution (1993)
Noble, M E, Zeelen, J P, Wierenga, R K, Mainfroid, V, Goraj, K, Gohimont, A C, ...
The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first...
Cloning of a human GHF-1/Pit-1 cDNA variant (1993)
Pernasetti, Flavia, Wera, Stefaan, Belayew, Alexandra, Martial, Joseph
Rentier-Delrue, Françoise, Mande, Shekhar C, Moyens, Sylvianne, Terpstra, Peter, Mainfroid, Véronique, Goraj, Karine, ...
We focused on the temperature adaptation of triosephosphate isomerase (TIM; E.C. 5.3.1.1.) by comparing the structure of TIMs isolated from bacterial organisms living in either cold or hot...
Rentier-Delrue, Françoise, Moyens, Sylvianne, Lion, Michelle, Martial, Joseph
By analysis of genomic clones, we have determined the complete nucleotide sequence of the gene encoding triosephosphate isomerase (TIM; EC 5.3.1.1) in the thermophilic bacterium, Bacillus...
Wera, S, Belayew, A, Martial, Joseph
Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL...
De Moerlooze, L, Lecomte, C, Brown-Shimmer, S, Schmetz, D, Guiot, C, Vandenbergh, D, ...
The nucleotide sequence of the cytopathic Osloss isolate of bovine viral diarrhoea virus (BVDV) was deduced from overlapping cDNA clones and from PCR products. The Osloss genome is an RNA molecule of...
Molecular Biology of tilapia prolactins (1993)
Swennen, E, Sekkali, B, Poncelet, AC, Rentier-Delrue, Françoise, Martial, Joseph, Belayew, A
Argenton, F, Vianello, S, Bernardini, S, Jacquemin, P, Martial, Joseph, Belayew, A, ...
Growth hormone (GH) gene expression in mammals is regulated by the interaction of the transcription factor Pit-1 with two binding sites within the proximal promoter. Four sequences, homologous to the...
Bellefroid, Eric J, Marine, Jean-Christophe, Ried, Thomas, Lecocq, Pierre J, Riviere, Michele, Amemiya, Chris, ...
KRAB zinc-finger proteins (KRAB-ZFPs) constitute a large subfamily of ZFPs of the Krüppel C2H2 type. KRAB (Krüppel-associated box) is an evolutionarily conserved protein domain found N-terminally...
Stabilization of T7-Promoter-Based Parhs Expression Vectors Using the Parb Locus (1992)
De Moerlooze, L, Struman, Ingrid, Renard, Andre, Martial, Joseph
We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to...
Geenen, Vincent, Robert, F, Legros, Jean-Jacques, Defresne, Marie-Paule, Boniver, Jacques, Martial, Joseph, ...
Localization of the human KRAB finger gene ZNF117 (HPF9) to chromosome 7q11.2 (1992)
Bellefroid, Eric J, Ried, Thomas, Riviere, Michele, Marine, Jean-Christophe, Levan, G, Szpirer, J, ...
A cluster of Kruppel type zinc finger genes of the KRAB subclass has recently been localized on human chromosome 19p12-p13.1. We now report that ZNF117 (HPF9), a closely related zinc finger gene of...
Goffin, V, Norman, M, Martial, Joseph
We have generated 10 alanine mutants of human PRL (hPRL), a member of the PRL/GH family, to investigate the involvement of the highly conserved 58-74 region in the biological behavior of the protein....
Peers, Bernard, Nalda, Asunción M, Monget, Philippe, Voz, Marianne, Belayew, Alexandra, Martial, Joseph
cAMP strongly stimulates the activity of the human prolactin (hPRL) promoter. We have previously shown that two types of cis-element are required for this cAMP regulation; binding sites for the...
Structure of the tilapia (Oreochromis mossambicus) prolactin I gene (1992)
Swennen, D, Poncelet, AC, Sekkali, B, Rentier-Delrue, Françoise, Martial, Joseph, Belayew, A
The tilapia (Oreochromis mossambicus) prolactin-I (PRL-I) gene has been cloned and sequenced. Its transcript (3,677 bases long) begins with a guanine and is organized in five exons and four introns...
De Moerlooze, L, Lecomte, C, Renard, A, Vandenbergh, D, Vanderheijden, N, Martial, Joseph
Proceedings of the second symposium on pestiviruses
Beauregard, Marc, Goraj, Karine, Goffin, Vincent, Heremans, Karel, Goormaghtigh, Eric, Ruysschaert, Jean-Marie, ...
We present here a spectroscopic structural characterization of octarellin, a recently reported de novo protein modelled on alpha/beta-barrel proteins [K. Goraj, A. Renard and J.A. Martial (1990)...
Bellefroid, Eric J, Poncelet, Dominique A, Lecocq, Pierre J, Revelant, Olivier, Martial, Joseph
We have previously shown that the human genome includes hundreds of genes coding for putative factors related to the Kruppel zinc-finger protein, which regulates Drosophila segmentation. We report...
Berwaer, M, Monget, P, Peers, Bernard, Mathy, Marianne, Bellefroid, E, Davis, J R, ...
We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown...
Pit-1 binding sequences permit calcium regulation of human prolactin gene expression (1991)
Hoggard, Nigel, Davis, Julian R E, Berwaer, Monique, Monget, Philippe, Peers, Bernard, Belayew, Alexandra, ...
This study examines the regulation of the human PRL (hPRL) gene promoter by intracellular calcium. Deletants of the 5'-flanking region of the hPRL gene and constructs consisting of the thymidine...
Swennen, D, Rentier-Delrue, Françoise, Auperin, B, Prunet, P, Flik, G, Wendelaar Bonga, S E, ...
Recombinant expression vectors carrying tilapia prolactin-I or -II (tiPRL-I or tiPRL-II) cDNA were constructed and the tiPRL-I and II proteins were produced in E. coli as inclusion bodies. These...
Neuroendocrinology of the thymus (1991)
Geenen, Vincent, Robert, Françoise, Martens, Henri, Benhida, Abdellah, De Giovanni, Gérard, Defresne, Marie-Paule, ...
Fonds de la Recherche Scientifique (Communauté française de Belgique) - FNRS
Geenen, Vincent, Robert, Françoise, Legros, Jean-Jacques, Defresne, Marie-Paule, Boniver, Jacques, Martial, Joseph, ...
In recent years, it appeared more and more that the three major integrating and adaptive systems of intercellular communication, nervous, endocrine, and immune systems, are closely interconnected....
Thymic cryptocrine signaling and the immune recognition of self neuroendocrine functions (1991)
Geenen, Vincent, Martens, Henri, Robert, Françoise, Legros, Jean-Jacques, Degiovanni, Gérard, Defresne, Marie-Paule, ...
De Moerlooze, L, Desport, M, Renard, A, Lecomte, C, Brownlie, J, Martial, Joseph
We sequenced cDNAs, amplified by the polymerase chain reaction (PCR), which correspond to the carboxy-terminal portion of the 54-kDa protein of various cytopathic (cp) or noncytopathic (ncp)...
Goraj, Karine, Renard, André, Martial, Joseph
We have attempted to construct an artificial polypeptide that folds like the eight-stranded parallel beta-barrel structures. Our approach consists of repeating eight times a unit peptide designed to...
Expression in Escherichia coli of two mutated genes encoding the cholera toxin B subunit (1990)
L'Hoir, C, Renard, A, Martial, Joseph
To allow subsequent genetically mediated fusion of foreign antigens to cholera toxin B subunit (CTB), two mutated CTB encoding genes (ctxB) were constructed and overexpressed in Escherichia coli. The...
Triiodothyronine inhibits transcription from the human growth hormone promoter (1990)
Morin, A, Louette, J, Voz, Marianne, Tixier-Vidal, A, Belayew, A, Martial, Joseph
Three DNA constructs, the natural human growth hormone gene (hGH-hGH) its 500 bp promoter linked to the chloramphenicol acetyl transferase reporter gene (hGH-CAT), and its structural part linked to...
Bacterial production and purification of recombinant human prolactin (1990)
Paris, N, Rentier-Delrue, Françoise, Defontaine, A, Goffin, V, Lebrun, J, Mercier, L, ...
Escherichia coli cells transformed with a recombinant plasmid (pT7L) containing the coding sequence of human prolactin (hPrl) expressed a new protein. This protein, comigrating with human Prl on...
Pagesy, Patrick, Li, Jacques Y., Rentier-Delrue, Françoise, Delalande, Olivier, Le Bouc, Yves, Kujas, Michèle, ...
Some patients with active acromegaly have elevated plasma IGF-I concentrations with only minimal elevation of plasma GH. We compared adenomatous GH and SRIH expression in 3 such patients (patients...
The human genome contains hundreds of genes coding for finger proteins of the Kruppel type (1989)
Bellefroid, E J, Lecocq, P, Benhida, A, Poncelet, D, Belayew, A, Martial, Joseph
Our aim was to identify new human proteins with potential DNA binding activity, related to the Kruppel protein which regulates Drosophila segmentation. We screened a human placenta cDNA library and a...
Laverriere, J N, Richard, J L, Buisson, N, Martial, Joseph, Tixier-Vidal, A, Gourdji, D
The stimulation of PRL gene transcription by TRH involves the two branches of the phosphatidyl inositol pathway as shown by pharmacological mobilization of intracellular Ca2+ stores and activation of...
Lemaigre, F P, Peers, Bernard, Lafontaine, D.A., Mathy, Marianne, Rousseau, G G, Belayew, A, ...
The human genes coding for growth hormone (GH), chorionic somatomammotropin (placental lactogen, CS), and prolactin (Prl) are related evolutionarily but are expressed in phenotypically distinct cell...
Peptide hormones: from the transcription of the gene to the final product--a biochemical view (1989)
This very short review aims at analyzing the current biochemical view of the molecular steps involved in the peptide hormone synthesis, going from the gene to the final active peptide.
Rainbow trout prolactin cDNA cloning in Escherichia coli (1989)
Mercier, L, Rentier-Delrue, Françoise, Swennen, D, Lion, M, Le Goff, P, Prunet, P, ...
We describe the isolation and characterization of a cDNA for trout prolactin (tPrl). An extensive analysis of tPrl recombinant clones by restriction analysis and sequencing revealed the presence of...
Evidence of pre-prosomatostatin mRNA in human normal and tumoral anterior pituitary gland (1989)
Pagesy, P, Li, J Y, Rentier-Delrue, Françoise, Le Bouc, Y, Martial, Joseph, Peillon, F
Expression of the SRIH gene was investigated in six human normal anterior pituitaries, six GH-, three PRL-, three mixed GH/PRL-secreting and four nonsecreting adenomas. Total cellular RNA and...
Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli (1989)
Rentier-Delrue, Françoise, Swennen, D, Philippart, Jean-Claude, L'Hoir, C, Lion, M, Benrubi, O, ...
A cDNA library was prepared from poly(A)+RNA extracted from tilapia Oreochromis niloticus anterior pituitaries. The recombinant clones carrying the cDNA sequence of tilapia growth hormone (tiGH) were...
Tilapia prolactin: molecular cloning of two cDNAs and expression in Escherichia coli (1989)
Rentier-Delrue, Françoise, Swennen, D, Prunet, P, Lion, M, Martial, Joseph
We have isolated cDNA clones encoding tilapia prolactin (tiPRL) from a cDNA library prepared from tilapia (Oreochromis niloticus) anterior pituitary glands. A trout PRL cDNA fragment was used as...
Rentier-Delrue, Françoise, Swennen, D, Mercier, L, Lion, M, Benrubi, O, Martial, Joseph
We constructed a cDNA library using mRNA isolated from rainbow trout pituitaries. Two types of cDNA clones encoding growth hormone (GH) were isolated and their complete nucleotide sequences...
Muller, Marc, Martial, Joseph, Verly, W G
5-Bromo-2'-deoxyuridine triphosphate (Br-dUTP) and dTTP are used interchangeably for DNA synthesis in vitro by the Klenow fragment of Escherichia coli DNA polymerase I. When DNA containing Br-dUMP...
Renard, A, Lecomte, C, Rentier-Delrue, Françoise, Martial, Joseph
Use of Somatotropin in Livestock Production
Hooghe-Peters, E L, Belayew, A, Herregodts, P, Velkeniers, B, Smets, G, Martial, Joseph, ...
Data are controversial concerning the time when PRL-synthesizing cells are detected for the first time in the rat pituitary. Using a very sensitive immunocytochemical technique, we could visualize...
Laverriere, Jean-Noël, Tixier-Vidal, Andrée, Buisson, Nicole, Morin, Annie, Martial, Joseph, Gourdji, Danielle
TRH induces two separate events in pituitary PRL cells. It increases the release of stored PRL and enhances the rate of PRL gene transcription, which results in an increased steady state...
Acromegaly with low plasma GH level. A clinical biological, histological and GH mRNA study (1988)
Pagesy, P, Martial, Joseph, Delalande, O, Rentier-Delrue, Françoise, Derome, P, Frankenne, F, ...
Duez, Colette, Piron-Fraipont, Claudine, Joris, Bernard, Dusart, Jean, Urdea, Mickey S, Martial, Joseph, ...
Duez, Colette, Piron-Fraipont, Claudine, Joris, Bernard, Dusart, Jean, Urdea, Mickey S, Martial, Joseph, ...
An 11450-base DNA fragment containing the gene for the extracellular active-site serine DD-peptidase of Streptomyces R61 was cloned in Streptomyces lividans using the high-copy-number plasmid pIJ702...
Lecomte, CM, Renard, A, Martial, Joseph
From a human pituitary cDNA library, we have cloned 3 distinct human growth hormone (hGH) cDNAs, coding respectively for the 22 K hGH, the 20 K variant, and a yet unknown 17.5 K variant. S1 mapping...
Rajpert, E J, Lemaigre, F P, Eliard, P H, Place, M, Lafontaine, DA, Economidis, I V, ...
Modulation of gene expression by glucocorticoids involves interaction of these hormones with an intracellular receptor followed by 'transformation' of the hormone-receptor complex into a nuclear...
Molecular cloning of the bovine viral diarrhea virus genomic RNA (1987)
Renard, A, Schmetz, D, Guiot, C, Brown-Shimmer, S, Dagenais, L, Pastoret, Paul-Pierre, ...
The genomic RNA from Bovine Viral Diarrhea Virus (BVD) was cloned in E. coli. The complete sequence has been obtained and the genomic organization has been deduced. Some of the BVD specific cDNA have...
EXPRESSION OF THE GROWTH HORMONE VARIANT GENE IN HUMAN PLACENTA (1987)
FRANKENNE, Francis, Rentier-Delrue, Françoise, Scippo, Marie-Louise, Martial, Joseph, Hennen, Georges
Besides the hGH-N gene, which codes for the pituitary 22 and 20K GH variants, the human genome contains a second GH gene, namely the GH-V, which has been thought to be silent. We recently discovered...
Molecular cloning, sequencing and expression of BVDV RNA (1987)
Renard, A, Brown-Shimmer, S, Schmetz, D, Guiot, C, Dagenais, L, Pastoret, Paul-Pierre, ...
Pestivirus Infections of Ruminants
[Regulation of prolactin and growth hormone gene expression in pituitary cell culture] (1986)
Laverriere, J N, Tixier-Vidal, A, Morin, Alexis, Buisson, N, Muller, Marc, Truong, A T, ...
The rat pituitary tumor derived cell lines of the "GH" family offer a fruitful model for studying the expressions of the prolactin (rPRL) and growth hormone (rGH) genes in basic and regulated states....
Belayew, A, Bellefroid, E, Berwaer, M, Dumoulin, M, Lambert, C, Lemaitre-Wathy, C, ...
We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and...
Barlow, John W, Voz, Marianne, Eliard, Pierre H, Mathy, Marianne, De Nayer, Philippe, Economidis, Ioannis V, ...
The intracellular receptor for thyroid hormone is a protein found in chromatin. Since thyroid hormone stimulates transcription of the growth hormone gene through an unknown mechanism, the hypothesis...
Dehottay, Philippe, Dusart, Jean, Duez, Colette, Lenzini, Mauro V, Martial, Joseph, Frère, Jean-Marie, ...
A 4.9-kb DNA fragment containing the bla gene for the extracellular beta-lactamase (BLA) of Streptomyces albus G was cloned in Streptomyces lividans using the conjugative, low-copy-number plasmid...
Laverriere, Jean-Noël, Muller, Marc, Buisson, Nicole, Tougard, Claude, Tixier-Vidal, Andrée, Martial, Joseph, ...
In order to assess the potential role of DNA methylation in the expression of rat PRL (rPRL) as compared to rat GH (rGH) gene, the cleavage patterns generated by the isoschizomeric restriction...
Location of transcriptional and regulatory sequences within the prolactin family genes (1986)
Belayew, A, Bellefroid, E, Berwaer, M, Dumoulin, M, Lambert, C, Lemaitre-Wathy, C, ...
Eliard, P H, Marchand, M, Rousseau, G G, Formstecher, P, Mathy, Marianne, Belayew, A, ...
An in vitro competition assay was used to investigate whether binding sites for the human glucocorticoid receptor occur in the human genes for growth hormone (hGH) and placental lactogen (chorionic...
Molecular cloning of bovine viral diarrhea viral sequences (1985)
Renard, A, Guiot, C, Schmetz, D, Dagenais, L, Pastoret, Paul-Pierre, Dina, D, ...
Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD...
Karin, M, Eberhardt, N L, Mellon, S H, Malich, N, Richards, R I, Slater, E P, ...
Expression of the rat growth hormone (rGH) gene in the pituitary and in cultured pituitary tumor cells is regulated by glucocorticoid hormones. After co-transfer of cloned DNA containing the rGH gene...
Cloning and structure analysis of the rat apolipoprotein A-I cDNA (1984)
Poncin, Jacques, Martial, Joseph, Gielen, Jacques
Apolipoprotein A-I, the major protein in mammalian high-density lipoprotein, acts as a cofactor for lecithin-cholesterol acyltransferase during the formation of cholesterol ester and as such, is...
Isolation and characterization of the human prolactin gene (1984)
Truong, Anh T, Duez, Colette, Belayew, Alexandra, Renard, André, Pictet, Raymond, Bell, Greame I, ...
Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by...
Regulatory sequences in the human prolactin gene (1984)
Martial, Joseph, Truong, A T, Eliard, P, Mathy, Marianne, Rousseau, G G, Belayew, A
Endocrinology: Proceedings of the 7th International Congress of Endocrinology
A method for isolation of intact, translationally active ribonucleic acid (1983)
Cathala, G, Savouret, J F, Mendez, B, West, B L, Karin, M, Martial, Joseph, ...
A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 M guanidine monothiocyanate followed by direct...
Laverriere, J N, Morin, A, Tixier-Vidal, A, Truong, A T, Gourdji, D, Martial, Joseph
The hypothalamic tripeptide thyroliberin (TRH) regulates prolactin (PRL) and growth hormone (GH) synthesis inversely by modulating the levels of their specific mRNA. Changes in mRNA levels could...
Molecular mechanisms of glucocorticoid hormone action (1982)
Lan, N C, Nguyen, T, Cathala, G, Wolff, M E, Kollman, P A, Wegnez, M, ...
Parks, J S, Herd, J E, Wurzel, J M, Martial, Joseph
Analysis of GH gene structure in the mouse permits evolutionary comparisons with GH gene structure in the rat and provides information about the mechanism responsible for heritable deficiencies of...
Structure and expression of hormone genes (1982)
Baxter, J D, Whitfield, P L, Seeburg, P H, Barta, A, Cooke, N E, Eberhardt, N L, ...
Structure et régulation du gène de la prolactine (1982)
Martial, Joseph, Laverriere, J N, Morin, A
Colloque de la Société nationale pour l'étude de la stérilité et de la fécondité
Structure et régulation du gène de la prolactine (1982)
Martial, Joseph, Laverriere, J N, Morin, A
INSERM 110
Human prolactin. cDNA structural analysis and evolutionary comparisons (1981)
Cooke, Nancy E, Coit, Doris, Shine, John, Baxter, John D, Martial, Joseph
Prolactin (Prl), growth hormone, and chorionic sommatomammotropin form a set (the "Prl set") of hormones which is thought to have evolved from a common ancestral gene. This assumption is based on...
Cloning of bovine prolactin cDNA and evolutionary implications of its sequence (1981)
Miller, W L, Coit, D, Baxter, J D, Martial, Joseph
Prolactin, growth hormone, and chorionic somatomammotropin (placental lactogen) constitute a set of related polypeptides believed to derive from a common evolutionary ancestor protein. We have cloned...
The prolactin gene is located on chromosome 6 in humans (1981)
Owerbach, D, Rutter, W J, Cooke, N E, Martial, Joseph, Shows, T B
The gene for prolactin has been located on chromosome 6 in humans. DNA fragments of 4.8 and 4.0 kilobases containing prolactin gene sequences were identified in human genomic DNA, whereas DNA...
Structure of cloned DNA complementary to rat prolactin messenger RNA (1980)
Cooke, Nancy E, Coit, Doris, Weiner, Richard I, Baxter, John D, Martial, Joseph
Cronin, Michaël J, Faure, Nacia, Martial, Joseph, Weiner, Richard I
Dopamine (DA) and DA agonists bind with high affinity to anterior pituitary receptors which mediate the inhibition of PRL release. Spiperone (SPIP), a DA antagonist, has also been successfully used...
Decreased responsiveness of GH3 cells to the dopaminergic inhibition of prolactin (1980)
Faure, Nacia, Cronin, Michaël J, Martial, Joseph, Weiner, Richard I
The inhibitory regulation of PRL secretion by dopamine (DA) or the dopaminergic agonists bromergocryptine (CB-154) and apomorphine was studied in cultured GH3 cells, an established rat anterior...
Cell-free synthesis of acetylcholine receptor polypeptides (1980)
Mendez, B, Valenzuela, P, Martial, Joseph, Baxter, J D
Messenger RNA coding for acetylcholine receptor peptides has been identified. This polyadenylate [poly(A)+]RNA from Torpedo californica directs, in a cell-free system, the synthesis of peptides...
Molecular cloning of DNA complementary to bovine growth hormone mRNA (1980)
Miller, Walter L, Martial, Joseph, Baxter, John D
We have cloned DNA complementary to mRNA coding for bovine growth hormone (bGH). Double-stranded DNA complementary to bovine pituitary mRNA was inserted into the Pst I site of plasmid pBR322 by the...
Cloning of DNA complementary to bovine prolactin mRNA (1980)
Miller, Walter L, Thirion, Jean-Paul, Martial, Joseph
We have cloned DNA complementary to mRNA coding for bovine prolactin (bPrl). Double-stranded cDNA prepared from bovine pituitary mRNA was inserted into the Pst I site of plasmid bPR322 by the dC x dG...
Owerbach, D, Rutter, W J, Martial, Joseph, Baxter, J D, Shows, T B
The human genes for growth hormone (GH), chorionic somatomammotropin (CSH), and a third growth hormone-like gene (GHL) have been located on chromosome 17 in humans. DNA fragments of 2.6, 2.8, and 9.5...
The pH dependence of rat liver RNA polymerases I and II. (1980)
Bull, P, Martial, Joseph, Téllez, R, Venegas, A, Valenzuela, P
The effect of pH on the stability and activity of rat liver RNA polymerases I (A) and II (B) has been studied. Both enzymes are irreversibly inactivated in buffer solutions below pH 5.0. Km values of...
Regulation of the genes for ACTH and growth hormone by glucocorticoid hormones (1980)
Johnson, L K, Eberhardt, N L, Spindler, S S, Martial, Joseph, Dallman, M F, Jones, M T, ...
Isolation and sequence analysis of the rat prolactin structural gene (1980)
International Symposium on Central and Peripheral Regulation of Prolactin Function, , 1979.
Thyroid hormone receptors and responses (1979)
Baxter, J D, Eberhardt, N L, Apriletti, J W, Johnson, L K, Ivarie, R D, Schachter, B S, ...
Human growth hormone: complementary DNA cloning and expression in bacteria (1979)
Martial, Joseph, Hallewell, R A, Baxter, J D, Goodman, H M
The nucleotide sequence of a DNA complementary to human growth hormone messenger RNA was cloned; it contains 29 nucleotides in its 5' untranslated region, the 651 nucleotides coding for the...
Regulation of growth hormone messenger RNA (1979)
Martial, Joseph, Seeburg, P H, Matulich, D T, Goodman, H M, Baxter, J D
In cultured rat pituitary cells, glucocorticoids regulate growth hormone production by modulating the number of growth hormone messenger RNA molecules. The effect is quite specific, since only a few...
Structure and expression in bacteria of growth hormone genes (1979)
GOODMAN, H M, SEEBURG, P H, Martial, Joseph, SHINE, J, ULLRICH, A, BAXTER, J D
Benzon Symposium
Synthesis of growth hormone by bacteria (1978)
Seeburg, P H, Shine, J, Martial, Joseph, Ivarie, R D, Morris, J A, Ullrich, A, ...
A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences...
Regulation of gene expression by thyroid hormones (1978)
LATHAM, K R, MACLEOD, K M, PAPAVASILIOU, S S, Martial, Joseph, SEEBURG, P H, GOODMAN, H M, ...
Subunits of yeast RNA polymerase I involved in interactions with DNA and nucleotides (1978)
Valenzuela, Pablo, Bull, Paulina, Zaldivar, Josefina, Venegas, Venegas, Martial, Joseph
Reaction of yeast RNA polymerase I with pyridoxal 5′-phosphate and sodium borohydride under conditions which inactivate the enzyme results in the specific binding of pyridoxal 5′-phosphate to...
Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones (1977)
Martial, Joseph, Baxter, John D, Goodman, Howard M, Seeburg, Peter H
Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells....
Martial, Joseph, Seeburg, Peter H, Guenzi, Doris, Goodman, Howard M, Baxter, John D
Cultured rat pituitary cells (GC) respond to thyroid and glucocorticoid hormones by increases in growth hormone production and growth hormone mRNA. When these cells are transferred from medium...
Thyroid hormonelike actions of 3,3',5'-L-triiodothyronine nad 3,3'-diiodothyronine (1977)
Papavasiliou, S S, Martial, Joseph, Latham, K R, Baxter, J D
l-Thyroxine is converted to 3,5,3'-l-triiodothyronine (T(3)) as well as to 3,3',5'-l-triiodothyronine (reverse T(3)). One product of further deiodination is 3,3'-diiodothyronine (3,3'T(2)). The serum...
Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone (1977)
Seeburg, P H, Shine, J, Martial, Joseph, Baxter, J D, Goodman, H M
The primary structure of DNA containing the sequence for rat pituitary growth hormone mRNA has been determined. DNA was obtained by reverse transcription of polyadenylated RNA from cultured pituitary...
Seeburg, Peter H, Shine, John, Martial, Joseph, Ullrich, Axel, Baxter, John D, Goodman, Howard M
A novel purification procedure for DNA complementary to individual mRNA species has been developed by using restriction endonuclease cleavage of cDNA transcribed from a complex mixture of mRNAs. This...
Construction and analysis of recombinant DNA for human chorionic somatomammotropin (1977)
Shine, J, Seeburg, P H, Martial, Joseph, Baxter, J D, Goodman, H M
DNA complementary to mRNA coding for the human polypeptide hormone, chorionic somatomammotropin, has been purified by specific restriction endonuclease digestion and religation before cloning into...
Rousseau, Guy, Martial, Joseph, De Visscher, M
We have examined whether glucocorticoids control the activity and (or) the subcellular distribution of protein kinase dependent on cyclic AMP (adenosine 3':5'-monophosphate), since they influence...
Martial, Joseph, Zaldivar, J, Bull, P, Venegas, A, Valenzuela, P
Purified DNA-dependent RNA polymerase forms I (A) and II (B) from rat liver and form I from yeast are rapidly inactivated by pyridoxal 5'-phosphate at pH 8.0. The inhibition is relatively specific...
Bull, Paulina, Zaldivar, Josefina, Venegas, Alejandro, Martial, Joseph, Valenzuela, Pablo
The inactivation of E. coli RNA polymerase (3.3 × 10−7M) by pyridoxal 5′-phosphate (1 × 10−4M to 5 × 10−4M) is a first order process with respect to the remaining active enzyme. Studies of...
Venegas, Alejandro, Martial, Joseph, Valenzuela, Pablo
DNA-dependent RNA polymerase from E. coli is rapidly inhibited by preincubation with pyridoxal 5′-phosphate. The enzyme is not inhibited by pyridoxamine 5′-phosphate or pyridoxine. Pyridoxal is...
Maes, Dominique, Crabeel, Marjolaine, Van De Weerdt, Cécile, Martial, Joseph, Peeters, Eveline, Charlier, Daniël, ...
A study on the crystallization of ornithine acetyltransferase from yeast, catalysing the fifth step in microbial arginine synthesis, is presented. The use of the counter-diffusion technique removes...