Expression of human α1-proteinase inhibitor in Aspergillus niger (2007)
Karnaukhova, Elena, Ophir, Yakir, Trinh, Loc, Dalal, Nimish, Punt, Peter J, Golding, Basil, ...
Abstract Background Human α 1 -proteinase inhibitor (α 1 -PI), also known as antitrypsin, is the most abundant serine protease inhibitor (serpin) in plasma. Its deficiency is associated with...
Jaluria, Pratik, Betenbaugh, Michael, Konstantopoulos, Konstantinos, Shiloach, Joseph
Abstract Background Genomics tools, particularly DNA microarrays, have found application in a number of areas including gene discovery and disease characterization. Despite the vast utility of these...
A perspective on microarrays: current applications, pitfalls, and potential uses (2007)
Jaluria, Pratik, Konstantopoulos, Konstantinos, Betenbaugh, Michael, Shiloach, Joseph
Abstract With advances in robotics, computational capabilities, and the fabrication of high quality glass slides coinciding with increased genomic information being available on public databases,...
Production of human α1proteinase inhibitor from Aspergillus niger (2006)
Chill, Liat, Trinh, Loc B, Karnaukhova, Elena, Ophir, Yakir, Golding, Basil, Shiloach, Joseph
No abstract available.
Postnikov, Yuri V., Lehn, Donald A., Robinson, Richard C., Frideman, Fred K., Shiloach, Joseph, Bustin, Michael
Mutants of human chromosomal protein HMG-14 were generated by site directed mutagenesis and used to study functional domains in this protein. A replacement of serine by cysteine at position 7 did not...
Recombinant human chromosomal proteins HMG-14 and HMG-17 (1991)
Bustin, Michael, Becerra, Patricia S., Crippa, Massimo P., Lehn, Donald A., Pash, James M., Shiloach, Joseph
Vectors for expressing human chromosomal proteins HMG-14 and HMG-17 in bacterial cultures under the control of the temperature-inducible λ PL promoter have been constructed. The open reading frames...
Bacillus subtilis contains multiple forms of somatostatin-like material (1985)
Leroith, Derek, Pickens, William, Vinik, Aaron I., Shiloach, Joseph
Extracts of B. subtilis contain somatostatin-like immunoactivity (1-20 pg per g wet weight cells). Two major forms were detected, one with reactivity in both N-and C- terminal immunoassays similar to...
Pavliakova, Danka, Chu, Chiayung, Bystricky, Slavomir, Tolson, Nathaniel W., Shiloach, Joseph, Kaufman, Jeanne B., ...
Seroepidemiological data and a clinical trial with a Shigella sonnei O-specific polysaccharide (O-SP)–Pseudomonas aeruginosa recombinant exoprotein A (rEPA) conjugate provide evidence that a...
Konadu, Edward, Donohue-Rolfe, Arthur, Calderwood, Stephen B., Pozsgay, Vince, Shiloach, Joseph, Robbins, John B., ...
Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers,...
Passwell, Justen H., Harlev, Efrat, Ashkenazi, Shai, Chu, Chiayung, Miron, Dan, Ramon, Reut, ...
Data suggest that the O-specific polysaccharide (O-SP) domain of the lipopolysaccharide (LPS) of Shigella species is both an essential virulence factor and a protective antigen and that a critical...
Kossaczka, Zuzana, Shiloach, Joseph, Johnson, Virginia, Taylor, David N., Finkelstein, Richard A., Robbins, John B., ...
Epidemiologic and experimental data provide evidence that a critical level of serum immunoglobulin G (IgG) antibodies to the surface polysaccharide of Vibrio cholerae O1 (lipopolysaccharide) and of...
Development of an improved vaccine for anthrax
Leppla, Stephen H., Robbins, John B., Schneerson, Rachel, Shiloach, Joseph
Schneerson, Rachel, Kubler-Kielb, Joanna, Liu, Teh-Yung, Dai, Zhong-Dong, Leppla, Stephen H., Yergey, Alfred, ...
Both the protective antigen (PA) and the poly(γ-d-glutamic acid) capsule (γdPGA) are essential for the virulence of Bacillus anthracis. A critical level of vaccine-induced IgG anti-PA confers...
Tsukiyama, Toshio, Palmer, Jeffrey, Landel, Carolyn C., Shiloach, Joseph, Wu, Carl
We have identified and characterized two Imitation Switch genes in Saccharomyces cerevisiae, ISW1 and ISW2, which are highly related to Drosophila ISWI, encoding the putative ATPase subunit of three...
Canh, Do Gia, Lin, Feng-ying (Kimi), Thiem, Vu Dinh, Trach, Dang Duc, Trong, Nguyen Dinh, Mao, Nguyen Duc, ...
In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 μg of Vi and...
Fritz, Timothy A., Hurley, James H., Trinh, Loc-Ba, Shiloach, Joseph, Tabak, Lawrence A.
UDP-GalNAc:polypeptide α-N-acetylgalactosaminyltransferases (ppGaNTases) initiate the formation of mucin-type, O-linked glycans by catalyzing the transfer of α-N-acetylgalactosamine from UDP-GalNAc...
The molecular structure of the Toll-like receptor 3 ligand-binding domain
Bell, Jessica K., Botos, Istvan, Hall, Pamela R., Askins, Janine, Shiloach, Joseph, Segal, David M., ...
Innate immunity is the first line of defense against invading pathogens. Toll-like receptors (TLRs) act as sentinels of the innate immune system, sensing a variety of ligands from lipopolysaccharide...
Jin, Zhigang, Bohach, Gregory A., Shiloach, Joseph, Norris, Scott E., Freedberg, Darón I., Deobald, Claudia, ...
Neisseria meningitidis groups A (GAM) and W135 capsular polysaccharides (CPs) were bound to recombinant Staphylococcus aureus enterotoxin C1 (rSEC). The CPs were activated with...
Pavliakova, Danka, Chu, Chiayung, Bystricky, Slavomir, Tolson, Nathaniel W., Shiloach, Joseph, Kaufman, Jeanne B., ...
Seroepidemiological data and a clinical trial with a Shigella sonnei O-specific polysaccharide (O-SP)–Pseudomonas aeruginosa recombinant exoprotein A (rEPA) conjugate provide evidence that a...
Konadu, Edward, Donohue-Rolfe, Arthur, Calderwood, Stephen B., Pozsgay, Vince, Shiloach, Joseph, Robbins, John B., ...
Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers,...
Passwell, Justen H., Harlev, Efrat, Ashkenazi, Shai, Chu, Chiayung, Miron, Dan, Ramon, Reut, ...
Data suggest that the O-specific polysaccharide (O-SP) domain of the lipopolysaccharide (LPS) of Shigella species is both an essential virulence factor and a protective antigen and that a critical...
Kossaczka, Zuzana, Shiloach, Joseph, Johnson, Virginia, Taylor, David N., Finkelstein, Richard A., Robbins, John B., ...
Epidemiologic and experimental data provide evidence that a critical level of serum immunoglobulin G (IgG) antibodies to the surface polysaccharide of Vibrio cholerae O1 (lipopolysaccharide) and of...
Development of an improved vaccine for anthrax
Leppla, Stephen H., Robbins, John B., Schneerson, Rachel, Shiloach, Joseph
Schneerson, Rachel, Kubler-Kielb, Joanna, Liu, Teh-Yung, Dai, Zhong-Dong, Leppla, Stephen H., Yergey, Alfred, ...
Both the protective antigen (PA) and the poly(γ-d-glutamic acid) capsule (γdPGA) are essential for the virulence of Bacillus anthracis. A critical level of vaccine-induced IgG anti-PA confers...
Tsukiyama, Toshio, Palmer, Jeffrey, Landel, Carolyn C., Shiloach, Joseph, Wu, Carl
We have identified and characterized two Imitation Switch genes in Saccharomyces cerevisiae, ISW1 and ISW2, which are highly related to Drosophila ISWI, encoding the putative ATPase subunit of three...
Canh, Do Gia, Lin, Feng-ying (Kimi), Thiem, Vu Dinh, Trach, Dang Duc, Trong, Nguyen Dinh, Mao, Nguyen Duc, ...
In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 μg of Vi and...
Fritz, Timothy A., Hurley, James H., Trinh, Loc-Ba, Shiloach, Joseph, Tabak, Lawrence A.
UDP-GalNAc:polypeptide α-N-acetylgalactosaminyltransferases (ppGaNTases) initiate the formation of mucin-type, O-linked glycans by catalyzing the transfer of α-N-acetylgalactosamine from UDP-GalNAc...
The molecular structure of the Toll-like receptor 3 ligand-binding domain
Bell, Jessica K., Botos, Istvan, Hall, Pamela R., Askins, Janine, Shiloach, Joseph, Segal, David M., ...
Innate immunity is the first line of defense against invading pathogens. Toll-like receptors (TLRs) act as sentinels of the innate immune system, sensing a variety of ligands from lipopolysaccharide...
Jin, Zhigang, Bohach, Gregory A., Shiloach, Joseph, Norris, Scott E., Freedberg, Darón I., Deobald, Claudia, ...
Neisseria meningitidis groups A (GAM) and W135 capsular polysaccharides (CPs) were bound to recombinant Staphylococcus aureus enterotoxin C1 (rSEC). The CPs were activated with...
A perspective on microarrays: current applications, pitfalls, and potential uses
Jaluria, Pratik, Konstantopoulos, Konstantinos, Betenbaugh, Michael, Shiloach, Joseph
With advances in robotics, computational capabilities, and the fabrication of high quality glass slides coinciding with increased genomic information being available on public databases, microarray...
Production of human α1 proteinase inhibitor from Aspergillus niger
Chill, Liat, Trinh, Loc B, Karnaukhova, Elena, Ophir, Yakir, Golding, Basil, Shiloach, Joseph
Kubler-Kielb, Joanna, Majadly, Fathy, Wu, Yimin, Narum, David L., Guo, Chunyan, Miller, Louis H., ...
Malaria is a leading cause of morbidity and mortality, estimated to cause >1 million childhood deaths annually. Plasmodium falciparum causes the most severe form of the disease. There is as yet no...
Extracellular structure of polysialic acid explored by on cell solution NMR
Azurmendi, Hugo F., Vionnet, Justine, Wrightson, Lauren, Trinh, Loc B., Shiloach, Joseph, Freedberg, Darón I.
The capsular polysaccharide of the pathogens Neisseria meningitidis serogroup B and of Escherichia coli K1, α(2 → 8) polysialic acid (PSA), is unusual, because when injected into adult humans, it...
Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction
White, Jim F., Grodnitzky, Justin, Louis, John M., Trinh, Loc B., Shiloach, Joseph, Gutierrez, Joanne, ...
G protein-coupled receptors (GPCRs) have been found as monomers but also as dimers or higher-order oligomers in cells. The relevance of the monomeric or dimeric receptor state for G protein...
Expression of human α1-proteinase inhibitor in Aspergillus niger
Karnaukhova, Elena, Ophir, Yakir, Trinh, Loc, Dalal, Nimish, Punt, Peter J, Golding, Basil, ...
Chu, Chia, Lugovtsev, Vladimir, Golding, Hana, Betenbaugh, Michael, Shiloach, Joseph
MDCK cells are currently being considered as an alternative to embryonated eggs for influenza virus propagation and hemagglutinin (HA) production intended for vaccine manufacturing. MDCK cells were...
Robbins, John B., Kubler-Kielb, Joanna, Vinogradov, Evguenii, Mocca, Christopher, Pozsgay, Vince, Shiloach, Joseph, ...
Shigellosis, an enteric disease, is on the World Health Organization's priority prevention list. In one study, the Shigella sonnei O-specific polysaccharide (O-SP)-protein conjugate showed 72%...