Joseph W. Lynch

Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry (2009)

Gilbert, Daniel, Wilson, John C., Nink, Virginia, Lynch, Joseph W., Osborne, Geoffrey W.

Flow cytometry is an important drug discovery tool because it permits high-content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing...

A Cation-π Interaction in the Binding Site of the Glycine Receptor Is Mediated by a Phenylalanine Residue (2008)

Pless, Stephan A., Millen, Kat S., Hanek, Ariele P., Lynch, Joseph W., Lester, Henry A., Lummis, Sarah C. R., ...

Cys-loop receptor binding sites characteristically contain many aromatic amino acids. In nicotinic ACh and 5-HT3 receptors, a Trp residue forms a cation-{pi} interaction with the agonist, whereas in...

Illuminating the structure and function of Cys-loop receptors (2008)

Pless, Stephan A, Lynch, Joseph W

Cys-loop receptors are an important class of ligand-gated ion channels. They mediate fast synaptic neurotransmission, are implicated in various 'channelopathies' and are important pharmacological...

Subunit-specific potentiation of recombinant glycine receptors by NV-31, a bilobalide-derived compound (2008)

Lynch, Joseph W., Chen, Xuebin

Bilobalide, a major bioactive component of Ginkgo biloba herbal extracts, exhibits neuroprotective and anti-ischaemic activity. However, its therapeutic potential is limited because of its...

Conformational variability of the glycine receptor M2 domain in response to activation by different agonists (2007)

Pless, Stephan A., Dibas, Mohammed I., Lester, Henry A., Lynch, Joseph W.

Models describing the structural changes mediating cys-loop receptor activation generally give little attention to the possibility that different agonists may promote activation via distinct M2...

A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABA(A) receptor chloride channels (2005)

Kruger, Wade, Gilbert, Daniel, Hawthorne, Rebecca, Hryciw, Deanne H., Frings, Stephan, Poronnik, Philip, ...

There is a significant clinical need to identify novel ligands with high selectivity and potency for GABA(A), GABA(C) and glycine receptor Cl- channels. Two recently developed, yellow fluorescent...

A picrotoxin-specific conformational change in the glycine receptor M2-M3 domain. (2005)

Hawthorne, Rebecca L., Lynch, Joseph W.

The external loop linking the M2 and M3 transmembrane domains is crucial for coupling agonist binding to channel gating in the glycine receptor chloride channel (GlyR). A substituted cysteine...

A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABA(A) receptor chloride channels (2005)

Kruger, Wade, Gilbert, Daniel, Hawthorne, Rebecca, Hryciw, Deanne H., Frings, Stephan, Poronnik, Philip, ...

There is a significant clinical need to identify novel ligands with high selectivity and potency for GABA(A), GABA(C) and glycine receptor Cl- channels. Two recently developed, yellow fluorescent...

A picrotoxin-specific conformational change in the glycine receptor M2-M3 loop. (2005)

Hawthorne, Rebecca L., Lynch, Joseph W.

The external loop linking the M2 and M3 transmembrane domains is crucial for coupling agonist binding to channel gating in the glycine receptor chloride channel (GlyR). A substituted cysteine...

Characterization of a glycine receptor domain that controls the binding and gating mechanisms of the beta-amino acid agonist, taurine (2001)

Han, Nian-Lin R., Haddrill, Justine L., Lynch, Joseph W.

The beta -amino acid, taurine, is a full agonist of the human glycine receptor al subunit when recombinantly expressed in a mammalian (HEK293) cell line, but a partial agonist of the same receptor...