Cardenas, Paula P., Carrasco, Begoña, Sanchez, Humberto, Deikus, Gintaras, Bechhofer, David H, Alonso, Juan C
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN...
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, (2008)
Helga Westers, Ronald Dorenbos, Jorrit Kabel, Tony Flanagan, Kevin M. Devine, ...
Reconstructing the Bacillus genome (Westers et al.) Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is...
Pratto, Florencia, Cicek, Aslan, Weihofen, Wilhelm A., Lurz, Rudi, Saenger, Wolfram, Alonso, Juan C.
The accurate partitioning of Firmicute plasmid pSM19035 at cell division depends on ATP binding and hydrolysis by homodimeric ATPase {delta}2 (ParA) and binding of {omega}2 (ParB) to its cognate parS...
Dynamic structures of Bacillus subtilis RecN-DNA complexes (2008)
Sanchez, Humberto, Cardenas, Paula P., Yoshimura, Shige H., Takeyasu, Kunio, Alonso, Juan C.
Genetic and cytological evidences suggest that Bacillus subtilis RecN acts prior to and after end-processing of DNA double-strand ends via homologous recombination, appears to participate in the...
Pratto, Florencia, Cicek, Aslan, Weihofen, Wilhelm A., Lurz, Rudi, Saenger, Wolfram, Alonso, Juan C.
The accurate partitioning of Firmicute plasmid pSM19035 at cell division depends on ATP binding and hydrolysis by homodimeric ATPase δ2 (ParA) and binding of ω2 (ParB) to its cognate parS DNA. The...
The RecU Holliday junction resolvase acts at early stages of homologous recombination (2008)
Cañas, Cristina, Carrasco, Begoña, Ayora, Silvia, Alonso, Juan C.
Homologous recombination is essential for DNA repair and generation of genetic diversity in all organisms. It occurs through a series of presynaptic steps where the substrate is presented to the...
Thaw, Paul, Sedelnikova, Svetlana E., Muranova, Tatyana, Wiese, Sebastian, Ayora, Sylvia, Alonso, Juan C., ...
The Lrp/AsnC family of transcriptional regulatory proteins is found in both archaea and bacteria. Members of the family influence cellular metabolism in both a global (Lrp) and specific (AsnC)...
Thaw, Paul, Sedelnikova, Svetlana E., Muranova, Tatyana, Wiese, Sebastian, Ayora, Sylvia, Alonso, Juan C., ...
Sanchez, Humberto, Alonso, Juan C.
Bacillus subtilis RecN appears to be an early detector of breaks in double-stranded DNA. In vivo, RecN forms discrete nucleoid-associated structures and in vitro exhibits Mg2+-dependent...
De La Hoz, Ana B., Pratto, Florencia, Misselwitz, Rolf, Speck, Christian, Weihofen, Wilhelm, Welfle, Karin, ...
pSM19035‐encoded ω protein forms a dimer (ω2) that binds to a set of 7‐bp repeats with sequence 5′‐NATCACN‐3′. Upon binding to its cognate sites, ω2 regulates transcription...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Canosa, Inés, López, Gema, Rojo, Fernando, Boocock, Martin R., Alonso, Juan C.
In the presence of a sequence‐independent chromatin‐associated protein, such as Hbsu or HMGB, the β recombinase catalyses resolution between two directly oriented recombination sites (six...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G40P, interacts with forked DNA (2002)
Ayora, Silvia, Weise, Frank, Mesa, Pablo, Stasiak, Andrzej, Alonso, Juan C.
SPP1-encoded replicative DNA helicase gene 40 product (G40P) is an essential product for phage replication. Hexameric G40P, in the presence of AMP-PNP, preferentially binds unstructured...
Tapias, Angels, Fernández, Silvia, Alonso, Juan C., Barbé, Jordi
Transcription of the Rhodobacter sphaeroides recA promoter (PrecA) is induced upon DNA damage in a lexA-dependent manner. In vivo experiments demonstrate that LexA protein represses and might also...
Martínez-Jiménez, María I., Mesa, Pablo, Alonso, Juan C.
Genetic evidence suggests that the Bacillus subtilis dnaX gene only encodes for the τ subunit of both DNA polymerases III (Pol IIIs). The B.subtilis full‐length protein and their mutant...
Proteolytic cleavage of Gram-positive ß recombinase is required for crystallization (1999)
Orth, Peter, Jekow, Petra, Alonso, Juan C., Hinrichs, Winfried
β Recombinase, a DNA resolvase-invertase, catalyzes in the presence of a chromatin-associated protein such as Hbsu, DNA resolution or DNA inversion on supercoiled substrates containing two directly...
Müller, Anna K., Rojo, Fernando, Alonso, Juan C.
Plasmlds control their copy number by limiting the amount of the Initiator for DNA replication. The plasmid pUB110 Initiator protein is termed repU. Expression of the pUB110 repU gene is controlled...
Rojo, Fernado, Alonso, Juan C.
The β recombinase from plasmid pSM19035 catalyzes Intramolecular site-specific recombination between two directly or Inversely oriented six sites In the presence of a chromatin-associated protein...
Rojo, Fernando, Alonso, Juan C.
The β protein encoded by the Streptococcus pyogenes plasmid pSM19035 is a site-specific recombinase involved in both resolution of plasmid multimers into monomers and DNA inversion. It has been...
Functional analysis of the bacillus subtilis bacteriophage SPP1 pac site (1990)
Bravo, Alicia, Alonso, Juan C., Trautner, Thomas A.
Encapsidation of the DNA of the virulent Bacillus subtilis phage SPP1 follows a processive unidirectional headful-mechanism and initiates at a unique genomic location {pac). We have cloned a fragment...
Alonso, Juan C., Shirahige, Katsuhiko, Ogasawara, Naotake
In Bacillus subtilis the recM gene, whose product is associated with DNA repair and recombination, has been located between the dnaX and rrnA genes. The recM gene has been cloned and analyzed....
Bravo, Alicia, Alonso, Juan C.
Bacteriophage SPP1 infection of Bacillus subtilis cells bearing plasmids induces the synthesis of multigenome-length plasmid molecules. Two independent pathways can account for this synthesis. In one...
Brantl, Sabine, Behnke, Detlev, Alonso, Juan C.
The large conjugative plasmid pIP501 was originally isolated from Streptococcus agalactiae. To study the molecular basis of pIP501 replication we determined the nucleotide sequence of a 2.2 kb DNA...
Revision of the nucleotide sequence of the Streptococcus pyogenes plasmid pSM19035 repS gene (1989)
Brantl, Sabine, Nowak, Angelika, Behnke, Detlev, Alonso, Juan C.
Viret, Jean-Francois, Alonso, Juan C.
The origin of lagging strand synthesis in pUB110, oriL, has been localized within 140 bases outside the pUB110 minimal replicon. The oriL DNA sequence is a cis-acting and orientation dependent...
Alonso, Juan C., Leonhardt, Heinrich, Stiege, Carola A.
Supercoiled plasmid DNA is the substrate for initiation of pUB110 replication, and - by inference - for binding of its initiator protein (RepU) to the plasmid replication origin (oriU) in vivo. No...
Generation of linear multigenome-length plasmid molecules in Bacillus subtilis (1987)
Viret, Jean-Francois, Alonso, Juan C.
Linear multigenome-length double and single stranded plasmid DNA was identified in a Bacillus subtilis ATP-dependent DNAase mutant strain (addA5) bearing plasmids pC194 or pBD95ts. Plasmid pBC30, a...
Actitudes de los maestros hacia la integración escolar de niños con necesidades especiales (1985)
García Sánchez, Jesús Nicasio, Alonso, Juan C.
This report presents a study of variables affecting teachers �attitudes towards mainstreaming children with special needs in 16 ordinary education schools in the province of Guipúzcoa. However, no...
Bacillus subtilis polynucleotide phosphorylase 3′-to-5′ DNase activity is involved in DNA repair
Cardenas, Paula P., Carrasco, Begoña, Sanchez, Humberto, Deikus, Gintaras, Bechhofer, David H, Alonso, Juan C
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN...
Structural basis for the nuclease activity of a bacteriophage large terminase
Smits, Callum, Chechik, Maria, Kovalevskiy, Oleg V, Shevtsov, Mikhail B, Foster, Andrew W, Alonso, Juan C, ...
The DNA-packaging motor in tailed bacteriophages requires nuclease activity to ensure that the genome is packaged correctly. This nuclease activity is tightly regulated as the enzyme is inactive for...
Evidence for Different Pathways during Horizontal Gene Transfer in Competent Bacillus subtilis Cells
Kidane, Dawit, Carrasco, Begoña, Manfredi, Candela, Rothmaier, Katharina, Ayora, Silvia, Tadesse, Serkalem, ...
Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA. The integration of homologous donor...