Pike, Jason E., Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
We have developed a system to reconstitute all of the proposed steps of Okazaki fragment processing using purified yeast proteins and model substrates. DNA polymerase δ was shown to extend an...
Human Dna2 is a nuclear and mitochondrial DNA maintenance protein (2009)
Duxin, Julien P., Dao, Benjamin, Martinsson, Peter, Rajala, Nina, Guittat, Lionel, Campbell, Judith L., ...
Dna2 is a highly conserved helicase/nuclease that in yeast participates in Okazaki fragment processing, DNA repair, and telomere maintenance. Here, we investigated the biological function of human...
Stewart, Jason A., Campbell, Judith L., Bambara, Robert A.
Okazaki fragments are initiated by short RNA/DNA primers, which are displaced into flap intermediates for processing. Flap endonuclease 1 (FEN1) and Dna2 are responsible for flap cleavage....
Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair (2009)
Budd, Martin E., Campbell, Judith L.
The Mre11/Rad50/Xrs2 complex initiates IR repair by binding to the end of a double-strand break, resulting in 5′ to 3′ exonuclease degradation creating a single-stranded 3′ overhang competent...
Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair (2009)
Budd, Martin E., Campbell, Judith L.
The Mre11/Rad50/Xrs2 complex initiates IR repair by binding to the end of a double-strand break, resulting in 5′ to 3′ exonuclease degradation creating a single-stranded 3′ overhang competent...
Stewart, Jason A., Miller, Adam S., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragments are initiated by an RNA/DNA primer, which is removed before the fragments are joined. Polymerase d displaces the primer into a flap for processing. Dna2 nuclease/helicase...
Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase delta (Pol delta) extends the upstream Okazaki fragment and displaces...
Lou, Huiqiang, Komata, Makiko, Katou, Yuki, Guan, Zhiyun, Reis, Clara C., Budd, Martin, ...
Yeast Mrc1, ortholog of metazoan Claspin, is both a central component of normal DNA replication forks and a mediator of the S phase checkpoint. We report that Mrc1 interacts with Pol2, the catalytic...
Masuda-Sasa, Taro, Polaczek, Piotr, Peng, Xiao P., Chen, Lu, Campbell, Judith L.
The polyguanine-rich DNA sequences commonly found at telomeres and in rDNA arrays have been shown to assemble into structures known as G quadruplexes, or G4 DNA, stabilized by base-stacked G...
Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase delta (Pol delta) extends the upstream Okazaki fragment and displaces...
Boronat, Susanna, Campbell, Judith L.
Ectopic expression of Cdc6p results in mitotic delay, and this has been attributed to Cdc6p-mediated inhibition of Cdc28 protein kinase and failure to activate the anaphase-promoting complex (APC)....
Masuda-Sasa, Taro, Polaczek, Piotr, Campbell, Judith L.
The Dna2 protein is a multifunctional enzyme with 5'-3' DNA helicase, DNA-dependent ATPase, 3' exo/endonuclease, and 5' exo/endonuclease. The enzyme is highly specific for structures containing...
Flap Endonuclease Disengages Dna2 Helicase/Nuclease from Okazaki Fragment Flaps (2006)
Stewart, Jason A., Campbell, Judith L., Bambara, Robert A.
Okazaki fragments contain an initiator RNA/DNA primer that must be removed before the fragments are joined. In eukaryotes, the primer region is raised into a flap by the strand displacement activity...
Budd, Martin E., Reis, Clara C., Smith, Stephanie, Myung, Kyungjae, Campbell, Judith L.
The precise machineries required for two aspects of eukaryotic DNA replication, Okazaki fragment processing (OFP) and telomere maintenance, are poorly understood. In this work, we present evidence...
Biochemical analysis of human Dna2 (2006)
Masuda-Sasa, Taro, Imamura, Osamu, Campbell, Judith L.
Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5' flaps. It is also involved in the...
Biochemical analysis of human Dna2 (2006)
Masuda-Sasa, Taro, Imamura, Osamu, Campbell, Judith L.
Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5′ flaps. It is also involved in...
A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability (2005)
Budd, Martin E., Tong, Amy Hin Yan, Polaczek, Piotr, Peng, Xiao, Boone, Charles, Campbell, Judith L.
To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately...
A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability (2005)
Martin E. Budd, Piotr Polaczek, Xiao Peng, Charles Boone, Judith L. Campbell
To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately...
Kao, Hui-I, Campbell, Judith L., Bambara, Robert A.
During cellular DNA replication the lagging strand is generated as discontinuous segments called Okazaki fragments. Each contains an initiator RNA primer that is removed prior to joining of the...
Lesur, Isabelle, Campbell, Judith L.
To help define the pathologies associated with yeast cells as they age, we analyzed the transcriptome of young and old cells isolated by elutriation, which allows isolation of biochemical quantities...
Imamura, Osamu, Campbell, Judith L.
Bloom syndrome is a disorder of profound and early cancer predisposition in which cells become hypermutable, exhibit high frequency of sister chromatid exchanges, and show increased micronuclei. BLM,...
Weitao, Tao, Budd, Martin, Mays Hoopes, Laura L., Campbell, Judith L.
We have proposed that faulty processing of arrested replication forks leads to increases in recombination and chromosome instability in Saccharomyces cerevisiae and contributes to the shortened...
Mutations in DNA Replication Genes Reduce Yeast Life Span (2002)
Mays Hoopes, Laura L., Budd, Martin, Choe, Wonchae, Weitao, Tao, Campbell, Judith L.
Surprisingly, the contribution of defects in DNA replication to the determination of yeast life span has never been directly investigated. We show that a replicative yeast helicase/nuclease, encoded...
Choe, Wonchae, Budd, Martin, Imamura, Osamu, Hoopes, Laura, Campbell, Judith L.
We have found that the Dna2 helicase-nuclease, thought to be involved in maturation of Okazaki fragments, is a component of telomeric chromatin. We demonstrate a dynamic localization of Dna2p to...
Dua, Rajiv, Levy, Daniel L., Li, Caroline M., Snow, Peter M., Campbell, Judith L.
DNA polymerase epsilon (pol epsilon) is a multiple subunit complex consisting of at least four proteins, including catalytic Po12p, Dpb2p, Dpb3p, and Dpb4p. Pol epsilon has been shown to play...
Dua, Rajiv, Edwards, Shaune, Levy, Daniel L., Campbell, Judith L.
Saccharomyces cerevisiae DNA polymerase epsilon (pol ε) is essential for chromosomal replication. A major form of pol ε purified from yeast consists of at least four subunits: Pol2p, Dpb2p, Dpb3p,...
Budd, Martin E., Choe, Won-chae, Campbell, Judith L.
Saccharomyces cerevisiae Dna2 protein is required for DNA replication and repair and is associated with multiple biochemical activities: DNA-dependent ATPase, DNA helicase, and DNA nuclease. To...
Liu, Qingquan, Choe, Won-chae, Campbell, Judith L.
The DNA2 gene of Saccharomyces cerevisiae is essential for growth and appears to be required for a late stage of chromosomal DNA replication. S. cerevisiae Dna2p (ScDna2p) is a DNA helicase and also...
Biopolymers: Protein and Nucleic Acids (2000)
Richards, John H., Abelson, John N., Hood, Leroy E., Simon, Melvin I., Campbell, Judith L.
The work focuses on learning the principles that govern interactions between proteins and nucleic acids. With these principles as guides we are synthesizing peptides (of about 50 amino acids) that...
Dua, Rajiv, Levy, Daniel L., Campbell, Judith L.
As first observed by Wittenberg (Kesti, T., Flick, K., Keranen, S., Syvaoja, J. E., and Wittenburg, C. (1999) Mol. Cell 3, 679-685), we find that deletion mutants lacking the entire N-terminal DNA...
Dua, Rajiv, Levy, Daniel L., Campbell, Judith L.
It has been proposed that C-terminal motifs of the catalytic subunit of budding yeast polymerase (pol) epsilon (POL2) couple DNA replication to the S/M checkpoint (Navas, T. A., Zheng, Z., and...
Budd, Martin E., Campbell, Judith L.
We have recently described a new helicase, the Dna2 helicase, that is essential for yeast DNA replication. We now show that the yeast FEN-1 (yFEN-1) nuclease interacts genetically and biochemically...
DNA2 Encodes a DNA Helicase Essential for Replication of Eukaryotic Chromosomes (1995)
Budd, Martin E., Choe, Won-Chae, Campbell, Judith L.
Although a number of eukaryotic DNA helicases have been identified biochemically and still more have been inferred from the amino acid sequences of the products of cloned genes, none of the cellular...
A Yeast Gene Required for DNA Replication Encodes a Protein with Homology to DNA Helicases (1995)
Budd, Martin E., Campbell, Judith L.
A yeast gene has been identified by screening for DNA replication mutants using a permeabilized cell replication assay. The mutant is temperature sensitive for growth and shows a cell cycle phenotype...
DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae (1995)
Budd, Martin E., Campbell, Judith L.
The ability of yeast DNA polymerase mutant strains to carry out repair synthesis after UV irradiation was studied by analysis of postirradiation molecular weight changes in cellular DNA. Neither DNA...
Photocopy of the microreproduction of original.
Regulation of Saccharomyces cerevisiae CDC7 function during the cell cycle (1993)
Yoon, Hye-Joo, Loo, Seng, Campbell, Judith L.
The yeast Cdc7 function is required for the G1/S transition and is dependent on passage through START, a point controlled by the Cdc28/cdc2/p34 protein kinase. CDC7 encodes a protein kinase activity,...
Budd, Martin E., Campbell, Judith L.
Three DNA polymerases, alpha, delta, and epsilon are required for viability in Saccharomyces cerevisiae. We have investigated whether DNA polymerases epsilon and delta are required for DNA...
The 66 kDa component of yeast SF1, stimulatory factor I, is hsp6O (1992)
Smiley, Jean K., Brown, William Clay, Campbell, Judith L.
DNA polymerase е stimulatory factor I (SFI) has been shown to contain three peptldes, p66, p37 and p13 Two of these components have been identified The p66 gene was cloned by using a p66...
Budd, Martin E., Wittrup, K. Dane, Bailey, J. E., Campbell, Judith L.
We have used a set of seven temperature-sensitive mutants in the DNA polymerase I gene of Saccharomyces cerevisiae to investigate the role of DNA polymerase I in various aspects of DNA synthesis in...
Saccharomyces cerevisiae SSB1 protein and its relationship to nucleolar RNA-binding proteins (1987)
Jong, Ambrose Y.-S., Clark, Michael W., Gilbert, Mary, Oehm, Alexander, Campbell, Judith L.
To better define the function of Saccharomyces cerevisiae SSB1, an abundant single-stranded nucleic acid-binding protein, we determined the nucleotide sequence of the SSB1 gene and compared it with...
Temperature-Sensitive Mutations in the Yeast DNA Polymerase I Gene (1987)
Budd, Martin, Campbell, Judith L.
Seven mutations that yield thermolabile DNA polymerases have been isolated in the DNA polymerase I gene, POL1, of Saccharomyces cerevisiae. Strains carrying the mutant genes are temperature sensitive...
Isolation of the Gene Encoding Yeast Single-Stranded Nucleic Acid Binding Protein 1 (1986)
Jong, Ambrose Y.-S., Campbell, Judith L.
A yeast gene encoding SSB-1, a single-stranded nucleic acid binding protein, has been isolated by screening a lambda gt11 genomic DNA library. The gene is located on a 1.84-kilobase chromosomal Bgl...
Effect of ARS1 mutations on chromosome stability in Saccharomyces cerevisiae (1985)
Srienc, Friedrich, Bailey, James E., Campbell, Judith L.
We have used a set of deletion mutations in the ARS1 element of Saccharomyces cerevisiae to measure their effect on chromosome stability. This work establishes the previously proposed existence of...
cis-acting mutations that affect rop protein control of plasmid copy number (1984)
Moser, David R., Ma, Doreen, Moser, Catherine D., Campbell, Judith L.
A number of pMB1 derivatives provide a trans-acting function that can suppress lethal runaway replication of a temperature-sensitive copy-number mutant of NTP1. Deletion analysis indicates that the...
Isolation of Yeast DNA Replication Mutants in Permeabilized Cells (1983)
Kuo, Chia-lam, Huang, Nak-hui, Campbell, Judith L.
A random population of temperature-sensitive mutants was screened by assaying for defects in DNA synthesis in a permeabilized yeast DNA replication system. Twenty mutants defective in in vitro DNA...
Kuo, Chia-Lam, Campbell, Judith L.
The CDC8 gene, whose product is required for DNA replication in Saccharomyces cerevisiae, has been isolated on recombinant plasmids. The yeast vector YCp50 bearing the yeast ARS1, CEN4, and URA3...
Moser, David R., Campbell, Judith L.
A 16-base-pair insertion has been identified as the mutation responsible for the high-copy-number phenotype of the plasmid copy number mutant pFH118. The mutation is located near the plasmid origin...
Kuo, Chia-Lam, Campbell, Judith L.
DNA synthesis in vitro in Brij-treated Saccharomyces cerevisiae requires the product of the CDC8 gene (Hereford, L. M. & Hartwell, L. H. (1971) Nature (London) New Biol. 234, 171-172). Extracts of...
Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids (1979)
Conrad, Susan E., Campbell, Judith L.
A modified in vitro replication system has been characterized and used to catalogue the host proteins required for the replication of plasmid RSF1030. These extracts differ from systems described...
Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids (1979)
Conrad, Susan E., Campbell, Judith L.
A modified in vitro replication system has been characterized and used to catalogue the host proteins required for the replication of plasmid RSF1030. These extracts differ from systems described...
Phosphorylation Controls Timing of Cdc6p Destruction: A Biochemical Analysis
Elsasser, Suzanne, Chi, Yong, Yang, Ping, Campbell, Judith L.
The replication initiation protein Cdc6p forms a tight complex with Cdc28p, specifically with forms of the kinase that are competent to promote replication initiation. We now show that potential...
Choe, Wonchae, Budd, Martin, Imamura, Osamu, Hoopes, Laura, Campbell, Judith L.
We have found that the Dna2 helicase-nuclease, thought to be involved in maturation of Okazaki fragments, is a component of telomeric chromatin. We demonstrate a dynamic localization of Dna2p to...
Mutations in DNA Replication Genes Reduce Yeast Life Span
Mays Hoopes, Laura L., Budd, Martin, Choe, Wonchae, Weitao, Tao, Campbell, Judith L.
Surprisingly, the contribution of defects in DNA replication to the determination of yeast life span has never been directly investigated. We show that a replicative yeast helicase/nuclease, encoded...
Edwards, Shaune, Li, Caroline M., Levy, Daniel L., Brown, Jessica, Snow, Peter M., Campbell, Judith L.
The large subunit of Saccharomyces cerevisiae DNA polymerase ɛ, Pol2, comprises two essential functions. The N terminus has essential DNA polymerase activity. The C terminus is also essential, but...
Imamura, Osamu, Campbell, Judith L.
Bloom syndrome is a disorder of profound and early cancer predisposition in which cells become hypermutable, exhibit high frequency of sister chromatid exchanges, and show increased micronuclei. BLM,...
Campbell, Judith L., Shizuya, Hiroaki, Richardson, Charles C.
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr × F− crosses, F-ductants in episome transfer, and transductants in...
The Transcriptome of Prematurely Aging Yeast Cells Is Similar to That of Telomerase-deficient Cells
Lesur, Isabelle, Campbell, Judith L.
To help define the pathologies associated with yeast cells as they age, we analyzed the transcriptome of young and old cells isolated by elutriation, which allows isolation of biochemical quantities...
Campbell, Judith L., Soll, Larry, Richardson, Charles C.
A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine and assay of polymerase activity in...
A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability
Budd, Martin E, Tong, Amy Hin Yan, Polaczek, Piotr, Peng, Xiao, Boone, Charles, Campbell, Judith L
To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately...
Biochemical analysis of human Dna2
Masuda-Sasa, Taro, Imamura, Osamu, Campbell, Judith L.
Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5′ flaps. It is also involved in...
Budd, Martin E., Reis, Clara C., Smith, Stephanie, Myung, Kyungjae, Campbell, Judith L.
The precise machineries required for two aspects of eukaryotic DNA replication, Okazaki fragment processing (OFP) and telomere maintenance, are poorly understood. In this work, we present evidence...
Phosphorylation Controls Timing of Cdc6p Destruction: A Biochemical Analysis
Elsasser, Suzanne, Chi, Yong, Yang, Ping, Campbell, Judith L.
The replication initiation protein Cdc6p forms a tight complex with Cdc28p, specifically with forms of the kinase that are competent to promote replication initiation. We now show that potential...
Choe, Wonchae, Budd, Martin, Imamura, Osamu, Hoopes, Laura, Campbell, Judith L.
We have found that the Dna2 helicase-nuclease, thought to be involved in maturation of Okazaki fragments, is a component of telomeric chromatin. We demonstrate a dynamic localization of Dna2p to...
Mutations in DNA Replication Genes Reduce Yeast Life Span
Mays Hoopes, Laura L., Budd, Martin, Choe, Wonchae, Weitao, Tao, Campbell, Judith L.
Surprisingly, the contribution of defects in DNA replication to the determination of yeast life span has never been directly investigated. We show that a replicative yeast helicase/nuclease, encoded...
Edwards, Shaune, Li, Caroline M., Levy, Daniel L., Brown, Jessica, Snow, Peter M., Campbell, Judith L.
The large subunit of Saccharomyces cerevisiae DNA polymerase ɛ, Pol2, comprises two essential functions. The N terminus has essential DNA polymerase activity. The C terminus is also essential, but...
Imamura, Osamu, Campbell, Judith L.
Bloom syndrome is a disorder of profound and early cancer predisposition in which cells become hypermutable, exhibit high frequency of sister chromatid exchanges, and show increased micronuclei. BLM,...
Campbell, Judith L., Shizuya, Hiroaki, Richardson, Charles C.
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr × F− crosses, F-ductants in episome transfer, and transductants in...
The Transcriptome of Prematurely Aging Yeast Cells Is Similar to That of Telomerase-deficient Cells
Lesur, Isabelle, Campbell, Judith L.
To help define the pathologies associated with yeast cells as they age, we analyzed the transcriptome of young and old cells isolated by elutriation, which allows isolation of biochemical quantities...
Campbell, Judith L., Soll, Larry, Richardson, Charles C.
A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine and assay of polymerase activity in...
A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability
Budd, Martin E, Tong, Amy Hin Yan, Polaczek, Piotr, Peng, Xiao, Boone, Charles, Campbell, Judith L
To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately...
Biochemical analysis of human Dna2
Masuda-Sasa, Taro, Imamura, Osamu, Campbell, Judith L.
Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5′ flaps. It is also involved in...
Budd, Martin E., Reis, Clara C., Smith, Stephanie, Myung, Kyungjae, Campbell, Judith L.
The precise machineries required for two aspects of eukaryotic DNA replication, Okazaki fragment processing (OFP) and telomere maintenance, are poorly understood. In this work, we present evidence...
Boronat, Susanna, Campbell, Judith L.
Ectopic expression of Cdc6p results in mitotic delay, and this has been attributed to Cdc6p-mediated inhibition of Cdc28 protein kinase and failure to activate the anaphase-promoting complex (APC)....
Reis, Clara C., Campbell, Judith L.
Balanced levels of histones are crucial for chromosome stability, and one major component of this control regulates histone mRNA amounts. The Saccharomyces cerevisiae poly(A) polymerases Trf4 and...
Jaszczur, Malgorzata, Flis, Krzysztof, Rudzka, Justyna, Kraszewska, Joanna, Budd, Martin E., Polaczek, Piotr, ...
Most replicases are multi-subunit complexes. DNA polymerase epsilon from Saccharomyces cerevisiae is composed of four subunits: Pol2p, Dpb2p, Dpb3p, and Dpb4p. Pol2p and Dpb2p are essential. To...
Interplay of Mre11 Nuclease with Dna2 plus Sgs1 in Rad51-Dependent Recombinational Repair
Budd, Martin E., Campbell, Judith L.
The Mre11/Rad50/Xrs2 complex initiates IR repair by binding to the end of a double-strand break, resulting in 5′ to 3′ exonuclease degradation creating a single-stranded 3′ overhang competent...
Masuda-Sasa, Taro, Polaczek, Piotr, Peng, Xiao P., Chen, Lu, Campbell, Judith L.
The polyguanine-rich DNA sequences commonly found at telomeres and in rDNA arrays have been shown to assemble into structures known as G quadruplexes, or G4 DNA, stabilized by base-stacked G...
Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase δ (Pol δ) extends the upstream Okazaki fragment and displaces the...
Stewart, Jason A., Miller, Adam S., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragments are initiated by a RNA/DNA primer, which is removed before the fragments are joined. Polymerase δ displaces the primer into a flap for processing. Dna2 nuclease/helicase...