Rhizobium common nod genes are required for biofilm formation (2008)
Fujishige, Nancy A, Lum, Michelle R, De Hoff, Peter L, Whitelegge, Julian P, Faull, Kym F, Hirsch, Ann M
In legume nitrogen-fixing symbioses, rhizobial nod genes are obligatory for initiating infection thread formation and root nodule development. Here we show that the common nod genes, nodD1ABC, whose...
Shaw, Bryan F., Schneider, Gregory F., Bilgiçer, Basar, Kaufman, George K., Neveu, John M., Lane, William S., ...
This paper reports that the acetylation of lysine ϵ-NH3 + groups of α-amylase—one of the most important hydrolytic enzymes used in industry—produces highly negatively charged variants that...
Proteomics and Mass Spectrometry for Cancer Biomarker Discovery (2007)
Ming Lu, Kym F. Faull, Julian P. Whitelegge, Jianbo He, Dejun Shen, Romaine E. Saxton, ...
Proteomics is a rapidly advancing field not only in the field of biology but also in translational cancer research. In recent years, mass spectrometry and associated technologies have been explored...
Sequencing covalent modifications of membrane proteins (2006)
Whitelegge, Julian P, Laganowsky, Arthur, Nishio, John, Souda, Puneet, Zhang, Huamin, Cramer, William A
A number of strategies have successfully extended plant proteomics into the bilayer domain. Important benefits can be afforded by including a well-resolved intact protein mass spectrum alongside...
Sequencing covalent modifications of membrane proteins (2006)
Whitelegge, Julian P., Laganowsky, Arthur, Nishio, John, Souda, Puneet, Zhang, Huamin, Cramer, William A.
A number of strategies have successfully extended plant proteomics into the bilayer domain. Important benefits can be afforded by including a well-resolved intact protein mass spectrum alongside...
Sequencing covalent modifications of membrane proteins (2006)
Whitelegge, Julian P., Laganowsky, Arthur, Nishio, John, Souda, Puneet, Zhang, Huamin, Cramer, William A.
A number of strategies have successfully extended plant proteomics into the bilayer domain. Important benefits can be afforded by including a well-resolved intact protein mass spectrum alongside...
Proteomic profiling of cancer biomarkers (2003)
Shau, Hungyi, Chandler, G. Scott, Whitelegge, Julian P., Gornbein, Jeffrey A., Faull, Kym F., Chang, Helena R.
Early detection and correct diagnosis are essential for effective treatment of cancer and patient survival. Complete sequencing of the human genome, and the genomes of other species, provides...
Blanco, David R., Whitelegge, Julian P., Miller, James N., Lovett, Michael A.
Purified native Tromp1 was subjected to mass spectrometric analysis in order to determine conclusively whether this protein possesses a cleaved or uncleaved signal peptide. The molecular masses of...
The Chloroplastic GrpE Homolog of Chlamydomonas: Two Isoforms Generated by Differential Splicing
Schroda, Michael, Vallon, Olivier, Whitelegge, Julian P., Beck, Christoph F., Wollman, Francis-André
In eubacteria and mitochondria, Hsp70 chaperone activity is controlled by the nucleotide exchange factor GrpE. We have identified the chloroplastic GrpE homolog of Chlamydomonas, CGE1, as an ∼26-kD...
Crystal structure of saposin B reveals a dimeric shell for lipid binding
Ahn, Victoria E., Faull, Kym F., Whitelegge, Julian P., Fluharty, Arvan L., Privé, Gilbert G.
Saposin B is a small, nonenzymatic glycosphingolipid activator protein required for the breakdown of cerebroside sulfates (sulfatides) within the lysosome. The protein can extract target lipids from...
Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry
Weinglass, Adam B., Whitelegge, Julian P., Hu, Yonglin, Verner, Gillian E., Faull, Kym F., Kaback, H.Ronald
Integration of biochemical and biophysical data on the lactose permease of Escherichia coli has culminated in a molecular model that predicts substrate–protein proximities which include interaction...
Identification of Proteins Associated with Murine Gammaherpesvirus 68 Virions
Bortz, Eric, Whitelegge, Julian P., Jia, Qingmei, Zhou, Z. Hong, Stewart, James P., Wu, Ting-Ting, ...
Murine gammaherpesvirus 68 (MHV68 [also known as γHV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying...
Yohannan, Sarah, Faham, Salem, Yang, Duan, Whitelegge, Julian P., Bowie, James U.
One of the hallmarks of membrane protein structure is the high frequency of transmembrane helix kinks, which commonly occur at proline residues. Because the proline side chain usually precludes...
Crother, Timothy R., Champion, Cheryl I., Whitelegge, Julian P., Aguilera, Rodrigo, Wu, Xiao-Yang, Blanco, David R., ...
The numbers of host-adapted Borrelia burgdorferi (HAB) organisms in rabbit skin were assessed by real-time PCR over the first 3 weeks of infection. Maximal numbers were found at day 11, while...
Blanco, David R., Champion, Cheryl I., Dooley, Alek, Cox, David L., Whitelegge, Julian P., Faull, Kym, ...
Immunization with purified Treponema pallidum outer membrane vesicles (OMV) has previously resulted in high-titer complement-dependent serum bactericidal activity. In this study, OMV immunization...
Kozak, Natalia A., Mattoo, Seema, Foreman-Wykert, Amy K., Whitelegge, Julian P., Miller, Jeff F.
We have recently described a multicomponent cascade that regulates type III secretion in Bordetella. This cascade includes a group of proteins, BtrU, BtrW, and BtrV, that contain an array of domains...
Barwe, Sonali P., Anilkumar, Gopalakrishnapillai, Moon, Sun Y., Zheng, Yi, Whitelegge, Julian P., Rajasekaran, Sigrid A., ...
The Na,K-ATPase, consisting of α- and β-subunits, regulates intracellular ion homeostasis. Recent studies have demonstrated that Na,K-ATPase also regulates epithelial cell tight junction structure...
Liu, Cuimin, Willmund, Felix, Whitelegge, Julian P., Hawat, Susan, Knapp, Bettina, Lodha, Mukesh, ...
J-domain cochaperones confer functional specificity to their heat shock protein (HSP)70 partner by recruiting it to specific substrate proteins. To gain insight into the functions of plastidic...
Blanco, David R., Whitelegge, Julian P., Miller, James N., Lovett, Michael A.
Purified native Tromp1 was subjected to mass spectrometric analysis in order to determine conclusively whether this protein possesses a cleaved or uncleaved signal peptide. The molecular masses of...
The Chloroplastic GrpE Homolog of Chlamydomonas: Two Isoforms Generated by Differential Splicing
Schroda, Michael, Vallon, Olivier, Whitelegge, Julian P., Beck, Christoph F., Wollman, Francis-André
In eubacteria and mitochondria, Hsp70 chaperone activity is controlled by the nucleotide exchange factor GrpE. We have identified the chloroplastic GrpE homolog of Chlamydomonas, CGE1, as an ∼26-kD...
Crystal structure of saposin B reveals a dimeric shell for lipid binding
Ahn, Victoria E., Faull, Kym F., Whitelegge, Julian P., Fluharty, Arvan L., Privé, Gilbert G.
Saposin B is a small, nonenzymatic glycosphingolipid activator protein required for the breakdown of cerebroside sulfates (sulfatides) within the lysosome. The protein can extract target lipids from...
Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry
Weinglass, Adam B., Whitelegge, Julian P., Hu, Yonglin, Verner, Gillian E., Faull, Kym F., Kaback, H.Ronald
Integration of biochemical and biophysical data on the lactose permease of Escherichia coli has culminated in a molecular model that predicts substrate–protein proximities which include interaction...
Identification of Proteins Associated with Murine Gammaherpesvirus 68 Virions
Bortz, Eric, Whitelegge, Julian P., Jia, Qingmei, Zhou, Z. Hong, Stewart, James P., Wu, Ting-Ting, ...
Murine gammaherpesvirus 68 (MHV68 [also known as γHV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying...
Yohannan, Sarah, Faham, Salem, Yang, Duan, Whitelegge, Julian P., Bowie, James U.
One of the hallmarks of membrane protein structure is the high frequency of transmembrane helix kinks, which commonly occur at proline residues. Because the proline side chain usually precludes...
Crother, Timothy R., Champion, Cheryl I., Whitelegge, Julian P., Aguilera, Rodrigo, Wu, Xiao-Yang, Blanco, David R., ...
The numbers of host-adapted Borrelia burgdorferi (HAB) organisms in rabbit skin were assessed by real-time PCR over the first 3 weeks of infection. Maximal numbers were found at day 11, while...
Barwe, Sonali P., Anilkumar, Gopalakrishnapillai, Moon, Sun Y., Zheng, Yi, Whitelegge, Julian P., Rajasekaran, Sigrid A., ...
The Na,K-ATPase, consisting of α- and β-subunits, regulates intracellular ion homeostasis. Recent studies have demonstrated that Na,K-ATPase also regulates epithelial cell tight junction structure...
Liu, Cuimin, Willmund, Felix, Whitelegge, Julian P., Hawat, Susan, Knapp, Bettina, Lodha, Mukesh, ...
J-domain cochaperones confer functional specificity to their heat shock protein (HSP)70 partner by recruiting it to specific substrate proteins. To gain insight into the functions of plastidic...
Blanco, David R., Champion, Cheryl I., Dooley, Alek, Cox, David L., Whitelegge, Julian P., Faull, Kym, ...
Immunization with purified Treponema pallidum outer membrane vesicles (OMV) has previously resulted in high-titer complement-dependent serum bactericidal activity. In this study, OMV immunization...
Kozak, Natalia A., Mattoo, Seema, Foreman-Wykert, Amy K., Whitelegge, Julian P., Miller, Jeff F.
We have recently described a multicomponent cascade that regulates type III secretion in Bordetella. This cascade includes a group of proteins, BtrU, BtrW, and BtrV, that contain an array of domains...
Nally, Jarlath E., Whitelegge, Julian P., Bassilian, Sara, Blanco, David R., Lovett, Michael A.
Pathogenic Leptospira species adapt to a wide range of environmental conditions during disease transmission and infection. While the proteome of in vitro cultivated Leptospira has been characterized...
Banci, Lucia, Bertini, Ivano, Durazo, Armando, Girotto, Stefania, Gralla, Edith Butler, Martinelli, Manuele, ...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder selectively affecting motor neurons; 90% of the total cases are sporadic, but 2% are associated with mutations in the...
Bortz, Eric, Wang, Lili, Jia, Qingmei, Wu, Ting-Ting, Whitelegge, Julian P., Deng, Hongyu, ...
The tegument, a semiordered matrix of proteins overlying the nucleocapsid and underlying the virion envelope, in viruses in the gamma subfamily of Herpesviridae is poorly understood. Murine...
Identities of Sequestered Proteins in Aggregates from Cells with Induced Polyglutamine Expression
Suhr, Steven T., Senut, Marie-Claude, Whitelegge, Julian P., Faull, Kym F., Cuizon, Denise B., Gage, Fred H.
Proteins with expanded polyglutamine (polyQ) tracts have been linked to neurodegenerative diseases. One common characteristic of expanded-polyQ expression is the formation of intracellular aggregates...
Amide bonds assemble pili on the surface of bacilli
Budzik, Jonathan M., Marraffini, Luciano A., Souda, Puneet, Whitelegge, Julian P., Faull, Kym F., Schneewind, Olaf
Pilin precursors are the building blocks of pili on the surface of Gram-positive bacteria; however, the assembly mechanisms of these adhesive fibers are unknown. Here, we describe the chemical bonds...
Shaw, Bryan F., Lelie, Herman L., Durazo, Armando, Nersissian, Aram M., Xu, Guillan, Chan, Pik K., ...
Determining the composition of aggregated superoxide dismutase 1 (SOD1) species associated with amyotrophic lateral sclerosis (ALS), especially with respect to co-aggregated proteins and...
Initiation and elongation in fibrillation of ALS-linked superoxide dismutase
Chattopadhyay, Madhuri, Durazo, Armando, Sohn, Se Hui, Strong, Cynthia D., Gralla, Edith B., Whitelegge, Julian P., ...
Familial amyotrophic lateral sclerosis (fALS) caused by mutations in copper–zinc superoxide dismutase (SOD1) is characterized by the presence of SOD1-rich inclusions in spinal cords. Similar...
Shaw, Bryan F., Schneider, Gregory F., Bilgiçer, Başar, Kaufman, George K., Neveu, John M., Lane, William S., ...
This paper reports that the acetylation of lysine ε-NH3 + groups of α-amylase—one of the most important hydrolytic enzymes used in industry—produces highly negatively charged variants that are...
Proteomics and Mass Spectrometry for Cancer Biomarker Discovery
Lu, Ming, Faull, Kym F., Whitelegge, Julian P., He, Jianbo, Shen, Dejun, Saxton, Romaine E., ...
Proteomics is a rapidly advancing field not only in the field of biology but also in translational cancer research. In recent years, mass spectrometry and associated technologies have been explored...
Averette, Kathleen M., Pratt, Matthew R., Yang, Yanan, Bassilian, Sara, Whitelegge, Julian P., Loo, Joseph A., ...
NOD-like receptors (NLRs) are a group of cytoplasmic molecules that recognize microbial invasion or ‘danger signals’. Activation of NLRs can induce rapid caspase-1 dependent cell death termed...