Iishi, Tatsuhiko, Hiraki, Takao, Mimura, Hidefumi, Gobara, Hideo, Kurose, Taichi, Fujiwara, Hiroyasu, ...
The present study was performed to clarify the effect of hypertonic saline infusion into the lung parenchyma on radiofrequency ablation (RFA) of the lungs. A total of 20 ablation zones were created...
CALABI-YAU THREEFOLDS OF QUOTIENT TYPE (2007)
Dedicated to Professor V. A. Iskovskih on the occasion of his sixtieth birthday Abstract. By a Calabi-Yau threefold we mean a minimal complex projective threefold X such that OX (KX) ' OX and h...
Radiofrequency ablation followed by radiation therapy for large primary lung tumors (2007)
Mukai, Takashi, Mimura, Hidefumi, Gobara, Hideo, Takemoto, Mitsuhiro, Himei, Kengo, Hiraki, Takao, ...
We report the clinical experience of radiofrequency ablation followed by radiation therapy for large primary lung tumors. Two patients with large primary lung tumors were treated with combined...
Clostridium perfringens Alpha-Toxin: Characterization and Mode of Action (2004)
Sakurai, Jun, Nagahama, Masahiro, Oda, Masataka
Clostridium perfringens type A strains that produce alpha-toxin cause gas gangrene, which is a life-threatening infection with fever, pain, edema, myonecrosis and gas production. Intramuscular...
Identification of Metal Ligands in the Clostridium histolyticum ColH Collagenase
Jung, Chang-Min, Matsushita, Osamu, Katayama, Seiichi, Minami, Junzaburo, Sakurai, Jun, Okabe, Akinobu
A Clostridium histolyticum 116-kDa collagenase has an H415EXXH motif but not the third zinc ligand, as found in already characterized zinc metalloproteinases. To identify its catalytic site, we...
Characterization of the Enzymatic Component of Clostridium perfringens Iota-Toxin
Nagahama, Masahiro, Sakaguchi, Yoshihiko, Kobayashi, Keiko, Ochi, Sadayuki, Sakurai, Jun
The iotaa component (ia) of Clostridium perfringens ADP ribosylates nonmuscle β/γ actin and skeletal muscle α-actin. Replacement of Arg-295 in ia with alanine led to a complete loss of...
Binding Component of Clostridium perfringens Iota-Toxin Induces Endocytosis in Vero Cells
Nagahama, Masahiro, Nagayasu, Koichi, Kobayashi, Keiko, Sakurai, Jun
Clostridium perfringens iota-toxin is a binary toxin consisting of two individual proteins, the binding component (Ib) and the enzyme component (Ia). Wild-type Ib bound to Vero cells at 4 and 37°C...
Existence of Two Distinct Hemolysins in Vibrio parahaemolyticus
Sakurai, Jun, Matsuzaki, Akiko, Takeda, Yoshifumi, Miwatani, Toshio
Two distinct hemolysins were demonstrated in Vibrio parahaemolyticus. A thermostable direct hemolysin purified from V. parahemolyticus WP-1, a Kanagawa phenomenon (KP)-positive strain, is...
Binding and Internalization of Clostridium perfringens Iota-Toxin in Lipid Rafts
Nagahama, Masahiro, Yamaguchi, Akiwo, Hagiyama, Tohko, Ohkubo, Noriko, Kobayashi, Keiko, Sakurai, Jun
Clostridium perfringens iota-toxin is a binary toxin composed of an enzymatic component (Ia) and a binding component (Ib). The oligomer of Ib formed in membranes induces endocytosis. We examined the...
Effect of Heat (Arrhenius Effect) on Crude Hemolysin of Vibrio parahaemolyticus
Miwatani, Toshio, Takeda, Yoshifumi, Sakurai, Jun, Yoshihara, Akiko, Taga, Sekiko
Crude hemolysins prepared from various strains of Vibrio parahaemolyticus, which give positive Kanagawa phenomenon, were partly inactivated by heating at 60 C, but not inactivated significantly by...
Purification and Characterization of Thermostable Direct Hemolysin of Vibrio parahaemolyticus
Sakurai, Jun, Matsuzaki, Akiko, Miwatani, Toshio
A thermostable direct hemolysin was purified from culture filtrates of Vibrio parahaemolyticus. The purified hemolysin gave one precipitation line with the antihemolysin antiserum on agar-gel...
Oda, Masataka, Ikari, Syusuke, Matsuno, Takayuki, Morimune, Yuka, Nagahama, Masahiro, Sakurai, Jun
Clostridium perfringens alpha-toxin induces the generation of superoxide anion (O2−) via production of 1,2-diacylglycerol (DG) in rabbit neutrophils. The mechanism of the generation, however,...
Identification of Metal Ligands in the Clostridium histolyticum ColH Collagenase
Jung, Chang-Min, Matsushita, Osamu, Katayama, Seiichi, Minami, Junzaburo, Sakurai, Jun, Okabe, Akinobu
A Clostridium histolyticum 116-kDa collagenase has an H415EXXH motif but not the third zinc ligand, as found in already characterized zinc metalloproteinases. To identify its catalytic site, we...
Characterization of the Enzymatic Component of Clostridium perfringens Iota-Toxin
Nagahama, Masahiro, Sakaguchi, Yoshihiko, Kobayashi, Keiko, Ochi, Sadayuki, Sakurai, Jun
The iotaa component (ia) of Clostridium perfringens ADP ribosylates nonmuscle β/γ actin and skeletal muscle α-actin. Replacement of Arg-295 in ia with alanine led to a complete loss of...
Binding Component of Clostridium perfringens Iota-Toxin Induces Endocytosis in Vero Cells
Nagahama, Masahiro, Nagayasu, Koichi, Kobayashi, Keiko, Sakurai, Jun
Clostridium perfringens iota-toxin is a binary toxin consisting of two individual proteins, the binding component (Ib) and the enzyme component (Ia). Wild-type Ib bound to Vero cells at 4 and 37°C...
Existence of Two Distinct Hemolysins in Vibrio parahaemolyticus
Sakurai, Jun, Matsuzaki, Akiko, Takeda, Yoshifumi, Miwatani, Toshio
Two distinct hemolysins were demonstrated in Vibrio parahaemolyticus. A thermostable direct hemolysin purified from V. parahemolyticus WP-1, a Kanagawa phenomenon (KP)-positive strain, is...
Binding and Internalization of Clostridium perfringens Iota-Toxin in Lipid Rafts
Nagahama, Masahiro, Yamaguchi, Akiwo, Hagiyama, Tohko, Ohkubo, Noriko, Kobayashi, Keiko, Sakurai, Jun
Clostridium perfringens iota-toxin is a binary toxin composed of an enzymatic component (Ia) and a binding component (Ib). The oligomer of Ib formed in membranes induces endocytosis. We examined the...
Effect of Heat (Arrhenius Effect) on Crude Hemolysin of Vibrio parahaemolyticus
Miwatani, Toshio, Takeda, Yoshifumi, Sakurai, Jun, Yoshihara, Akiko, Taga, Sekiko
Crude hemolysins prepared from various strains of Vibrio parahaemolyticus, which give positive Kanagawa phenomenon, were partly inactivated by heating at 60 C, but not inactivated significantly by...
Purification and Characterization of Thermostable Direct Hemolysin of Vibrio parahaemolyticus
Sakurai, Jun, Matsuzaki, Akiko, Miwatani, Toshio
A thermostable direct hemolysin was purified from culture filtrates of Vibrio parahaemolyticus. The purified hemolysin gave one precipitation line with the antihemolysin antiserum on agar-gel...
Oda, Masataka, Ikari, Syusuke, Matsuno, Takayuki, Morimune, Yuka, Nagahama, Masahiro, Sakurai, Jun
Clostridium perfringens alpha-toxin induces the generation of superoxide anion (O2−) via production of 1,2-diacylglycerol (DG) in rabbit neutrophils. The mechanism of the generation, however,...
Nagahama, Masahiro, Morimitsu, Shinsuke, Kihara, Atsushi, Akita, Masahiko, Setsu, Koujun, Sakurai, Jun
Clostridium perfringens beta-toxin causes dermonecrosis and oedema in the dorsal skin of animals. In the present study, we investigated the mechanisms of oedema induced by the toxin.The toxin induced...
Tsuge, Hideaki, Nagahama, Masahiro, Oda, Masataka, Iwamoto, Shinobu, Utsunomiya, Hiroko, Marquez, Victor E., ...
The ADP-ribosylating toxins (ADPRTs) produced by pathogenic bacteria modify intracellular protein and affect eukaryotic cell function. Actin-specific ADPRTs (including Clostridium perfringens...