Sennels, Lau, Bukowski-Wills, Jimi-Carlo, Rappsilber, Juri
Abstract Background Proteomic protein identification results need to be compared across laboratories and platforms, and thus a reliable method is needed to estimate false discovery rates. The...
Decourty, Laurence, Saveanu, Cosmin, Rappsilber, Juri, Jacquier, Alain, ...
A genome-wide screen for synthetic lethal (SL) interactions with loss of the nuclear exosome cofactors Rrp47/Lrp1 or Air1 identified 3' --> 35' exonucleases, the THO complex required for mRNP...
Protein abundance profiling of the Escherichia coli cytosol (2008)
Ishihama, Yasushi, Schmidt, Thorsten, Rappsilber, Juri, Mann, Matthias, Hartl, F Ulrich, Kerner, Michael J, ...
Abstract Background Knowledge about the abundance of molecular components is an important prerequisite for building quantitative predictive models of cellular behavior. Proteins are central...
Thomson, Emma, Rappsilber, Juri, Tollervey, David
Proteomic analyses in yeast have identified a large number of proteins that are associated with preribosomal particles. However, the product of the yeast ORF YJL010C, herein designated as Nop9,...
Lehmann, Wolf D., Wei, Junhua, Rappsilber, Juri, Salek, Mojiborahman
Upon low energy collision induced dissociation (CID), multiply protonated peptides generate a set of interdependent fragment ions detectable by MS/MS, the '[peptide]n+-fragmentome'. In particular...
eIF4A3 is a novel component of the exon junction complex (2004)
CHAN, CHIA C., DOSTIE, JOSÉE, DIEM, MICHAEL D., FENG, WENQIN, MANN, MATTHIAS, RAPPSILBER, JURI, ...
The exon junction complex (EJC) is a protein complex that assembles near exon–exon junctions of mRNAs as a result of splicing. EJC proteins play important roles in postsplicing events including...
Is mass spectrometry ready for proteome-wide protein expression analysis? (2002)
Rappsilber, Juri, Mann, Matthias
Abstract Recent advances in mass spectrometry will soon allow routine analysis of protein expression levels. How close are we to true quantitative proteomics?
Large-Scale Proteomic Analysis of the Human Spliceosome (2002)
Rappsilber, Juri, Ryder, Ursula, Lamond, Angus I., Mann, Matthias
Large-Scale Proteomic Analysis of the Human Spliceosome (2002)
Rappsilber, Juri, Ryder, Ursula, Lamond, Angus I., Mann, Matthias
Frankfurt (Main), Universiẗat, Diss., 2001.
Friesen, Westley J., Paushkin, Sergey, Wyce, Anastasia, Massenet, Severine, Pesiridis, G. Scott, Van Duyne, Gregory, ...
snRNPs, integral components of the pre-mRNA splicing machinery, consist of seven Sm proteins which assemble in the cytoplasm as a ring structure on the snRNAs U1, U2, U4, and U5. The survival motor...
Is mass spectrometry ready for proteome-wide protein expression analysis?
Rappsilber, Juri, Mann, Matthias
Recent advances in mass spectrometry will soon allow routine analysis of protein expression levels. How close are we to true quantitative proteomics?
Large-Scale Proteomic Analysis of the Human Spliceosome
Rappsilber, Juri, Ryder, Ursula, Lamond, Angus I., Mann, Matthias
In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we...
Model for stathmin/OP18 binding to tubulin
Wallon, Gerlind, Rappsilber, Juri, Mann, Matthias, Serrano, Luis
Stathmin/OP18 is a regulatory phosphoprotein that controls microtubule (MT) dynamics. The protein does not have a defined three-dimensional structure, although it contains three distinct regions (an...
eIF4A3 is a novel component of the exon junction complex
CHAN, CHIA C., DOSTIE, JOSÉE, DIEM, MICHAEL D., FENG, WENQIN, MANN, MATTHIAS, RAPPSILBER, JURI, ...
The exon junction complex (EJC) is a protein complex that assembles near exon–exon junctions of mRNAs as a result of splicing. EJC proteins play important roles in postsplicing events including...
miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
Mourelatos, Zissimos, Dostie, Josée, Paushkin, Sergey, Sharma, Anup, Charroux, Bernard, Abel, Linda, ...
Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6....
Friesen, Westley J., Paushkin, Sergey, Wyce, Anastasia, Massenet, Severine, Pesiridis, G. Scott, Van Duyne, Gregory, ...
snRNPs, integral components of the pre-mRNA splicing machinery, consist of seven Sm proteins which assemble in the cytoplasm as a ring structure on the snRNAs U1, U2, U4, and U5. The survival motor...
Is mass spectrometry ready for proteome-wide protein expression analysis?
Rappsilber, Juri, Mann, Matthias
Recent advances in mass spectrometry will soon allow routine analysis of protein expression levels. How close are we to true quantitative proteomics?
miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
Mourelatos, Zissimos, Dostie, Josée, Paushkin, Sergey, Sharma, Anup, Charroux, Bernard, Abel, Linda, ...
Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6....
Large-Scale Proteomic Analysis of the Human Spliceosome
Rappsilber, Juri, Ryder, Ursula, Lamond, Angus I., Mann, Matthias
In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we...
Model for stathmin/OP18 binding to tubulin
Wallon, Gerlind, Rappsilber, Juri, Mann, Matthias, Serrano, Luis
Stathmin/OP18 is a regulatory phosphoprotein that controls microtubule (MT) dynamics. The protein does not have a defined three-dimensional structure, although it contains three distinct regions (an...
eIF4A3 is a novel component of the exon junction complex
CHAN, CHIA C., DOSTIE, JOSÉE, DIEM, MICHAEL D., FENG, WENQIN, MANN, MATTHIAS, RAPPSILBER, JURI, ...
The exon junction complex (EJC) is a protein complex that assembles near exon–exon junctions of mRNAs as a result of splicing. EJC proteins play important roles in postsplicing events including...
Dietrich, Nikolaj, Bracken, Adrian P, Trinh, Emmanuelle, Schjerling, Charlotte K, Koseki, Haruhiko, Rappsilber, Juri, ...
The Polycomb group (PcG) proteins are essential for embryogenesis, and their expression is often found deregulated in human cancer. The PcGs form two major protein complexes, called polycomb...
Protein abundance profiling of the Escherichia coli cytosol
Ishihama, Yasushi, Schmidt, Thorsten, Rappsilber, Juri, Mann, Matthias, Hartl, F Ulrich, Kerner, Michael J, ...
A Functional Interaction between the Survival Motor Neuron Complex and RNA Polymerase II
Pellizzoni, Livio, Charroux, Bernard, Rappsilber, Juri, Mann, Matthias, Dreyfuss, Gideon
The survival motor neuron (SMN) protein, the protein product of the spinal muscular atrophy (SMA) disease gene, plays a role in the assembly and regeneration of small nuclear ribonucleoproteins...
Thomson, Emma, Rappsilber, Juri, Tollervey, David
Proteomic analyses in yeast have identified a large number of proteins that are associated with preribosomal particles. However, the product of the yeast ORF YJL010C, herein designated as Nop9,...
Molecular and Genetic Analysis of Condensin Function in Vertebrate Cells
Hudson, Damien F., Ohta, Shinya, Freisinger, Tina, MacIsaac, Fiona, Sennels, Lau, Alves, Flavia, ...
We engineered mutants into residues of SMC2 to dissect the role of ATPase function in the condensin complex. These residues are predicted to be involved in ATP binding or hydrolysis and in the...
Milligan, Laura, Decourty, Laurence, Saveanu, Cosmin, Rappsilber, Juri, Ceulemans, Hugo, Jacquier, Alain, ...
A genome-wide screen for synthetic lethal (SL) interactions with loss of the nuclear exosome cofactors Rrp47/Lrp1 or Air1 identified 3′→5′ exonucleases, the THO complex required for mRNP...
Fission Yeast Scm3: A CENP-A Receptor Required for Integrity of Subkinetochore Chromatin
Pidoux, Alison L., Choi, Eun Shik, Abbott, Johanna K.R., Liu, Xingkun, Kagansky, Alexander, Castillo, Araceli G., ...
The mechanisms ensuring specific incorporation of CENP-A at centromeres are poorly understood. Mis16 and Mis18 are required for CENP-A localization at centromeres and form a complex that is conserved...