Foor, F, Morin, N, Bostian, K A
The gene (tutA) encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high...
Encapsidation of yeast killer double-stranded ribonucleic acids: dependence of M on L.
Bostian, K A, Sturgeon, J A, Tipper, D J
Virus-like particles containing either L or M double-stranded ribonucleic acid (dsRNA) were isolated from a killer toxin-producing strains of Saccharomyces cerevisiae (K+ R+). At least 95% of M- and...
Viruses in fungi: infection of yeast with the K1 and K2 killer viruses.
We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of...
Hanes, S D, Burn, V E, Sturley, S L, Tipper, D J, Bostian, K A
The type I killer strains of Saccharomyces cerevisiae secrete a dimeric 19-kDa protein that kills sensitive cells by disrupting cytoplasmic membrane function. This toxin is encoded by the...
Saunders, C A, Bostian, K A, Halvorson, H O
Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo...
Bostian, K A, Burn, V E, Jayachandran, S, Tipper, D J
In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and...
Rogers, D T, Lemire, J M, Bostian, K A
Two clones from a lambda phage collection containing yeast genes regulated by inorganic phosphate were shown by low-stringency hybridization to select three mRNAs that direct the in vitro synthesis...
Bostian, K A, Lemire, J M, Cannon, L E, Halvorson, H O
Antibodies to repressible nonspecific acid phosphatase [APase; orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] purified from Saccharomyces cerevisiae were used to detect the in...
Brizuela, L, Chrebet, G, Bostian, K A, Parent, S A
FK-506 is a novel and potent antagonist of T-cell activation and an inhibitor of fungal growth. Its immunosuppressive activity can be antagonized by the structurally related antibiotic rapamycin, and...
Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
Lemire, J M, Willcocks, T, Halvorson, H O, Bostian, K A
We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in...
Tait-Kamradt, A G, Turner, K J, Kramer, R A, Elliott, Q D, Bostian, S J, Thill, G P, ...
We characterized the organization and expression of PHO5 and PHO3, the tightly linked repressible and constitutive acid phosphatase genes of Saccharomyces cerevisiae. The "constitutive" gene, PHO3,...
Thill, G P, Kramer, R A, Turner, K J, Bostian, K A
The nucleotide sequence of 5'-noncoding and N-terminal coding regions of two coordinately regulated, repressible acid phosphatase genes from Saccharomyces cerevisiae were determined. These unlinked...
Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.
Bostian, K A, Lemire, J M, Halvorson, H O
We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum], EC 3.1.3.2) in Saccharomyces cerevisiae at the physiological and...
El-Sherbeini, M, Tipper, D J, Mitchell, D J, Bostian, K A
The plasmid determinants of killer phenotypes in type K1 and K2 killer yeast cells are the 1.9-kilobase (kb) M1 and 1.7-kb M2 double-stranded RNAs (dsRNAs), respectively. These are dependent for...
Bostian, K A, Lemire, J M, Halvorson, H O
The synthesis of repressible acid phosphatase in Saccharomyces cerevisiae was examined under conditions of blocked derepression as described by Toh-e et al. (Mol. Gen. Genet. 162:139-149, 1978)....
Secretion of Saccharomyces cerevisiae Killer Toxin: Processing of the Glycosylated Precursor
Bussey, H., Saville, D., Greene, D., Tipper, D. J., Bostian, K. A.
Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of...
Effect of Newcastle Disease on Serum Copper, Zinc, Cholesterol, and Carotenoid Values in the Chick
Squibb, R. L., Beisel, W. R., Bostian, K. A.
Experimentally induced Newcastle disease virus infection of chicks, with a mortality index of 48%, was accompanied by increased concentrations in serum of copper and cholesterol and decreased...
Canonico, P G, Little, J S, Powanda, M C, Bostian, K A, Beisel, W R
Streptococcus pneumoniae infection leads to multifold increases in sialyltransferase, galactosyltransferase, alpha 2-fucosyltransferase, and alpha 3-fucosyltransferase activity of rat liver. Such...
Bostian, K A, Betts, G F, Man, W K, Hughes, M N
Univalent cation activators of aldehyde dehydrogenase have dual effects, both interpreted as cation-induced or -stabilized conformation changes. These two processes are differentiated by the time...
Betts, G F, Poole, P L, Springham, M G, Bostian, K A
The activity, stability and spectroscopic properties of yeast K+ -activated aldehyde dehydrogenase were measured at various times after removal from, and after returning to a solution containing K+....
Mapping of functional domains within the Saccharomyces cerevisiae type 1 killer preprotoxin.
Sturley, S L, Elliot, Q, LeVitre, J, Tipper, D J, Bostian, K A
Strains of Saccharomyces cerevisiae harboring M1-dsRNA, the determinant of type 1 killer and immunity phenotypes, secrete a dimeric 19-kd toxin that kills sensitive yeast cells by the production of...
A method for the purification of yeast K+-activated aldehyde dehydrogenase is presented which can be completed in substantially less time than other published procedures. The enzyme has a different...
Data from steady-state kinetic analysis of yeast K+-activated aldehyde dehydrogenase are consistent with a ternary complex mechanism. Evidence from alternative substrate analysis and...
Foor, F, Morin, N, Bostian, K A
The gene (tutA) encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high...
Encapsidation of yeast killer double-stranded ribonucleic acids: dependence of M on L.
Bostian, K A, Sturgeon, J A, Tipper, D J
Virus-like particles containing either L or M double-stranded ribonucleic acid (dsRNA) were isolated from a killer toxin-producing strains of Saccharomyces cerevisiae (K+ R+). At least 95% of M- and...
Viruses in fungi: infection of yeast with the K1 and K2 killer viruses.
We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of...
Hanes, S D, Burn, V E, Sturley, S L, Tipper, D J, Bostian, K A
The type I killer strains of Saccharomyces cerevisiae secrete a dimeric 19-kDa protein that kills sensitive cells by disrupting cytoplasmic membrane function. This toxin is encoded by the...
Saunders, C A, Bostian, K A, Halvorson, H O
Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo...
Bostian, K A, Burn, V E, Jayachandran, S, Tipper, D J
In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and...
Rogers, D T, Lemire, J M, Bostian, K A
Two clones from a lambda phage collection containing yeast genes regulated by inorganic phosphate were shown by low-stringency hybridization to select three mRNAs that direct the in vitro synthesis...
Bostian, K A, Lemire, J M, Cannon, L E, Halvorson, H O
Antibodies to repressible nonspecific acid phosphatase [APase; orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] purified from Saccharomyces cerevisiae were used to detect the in...
Brizuela, L, Chrebet, G, Bostian, K A, Parent, S A
FK-506 is a novel and potent antagonist of T-cell activation and an inhibitor of fungal growth. Its immunosuppressive activity can be antagonized by the structurally related antibiotic rapamycin, and...
Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
Lemire, J M, Willcocks, T, Halvorson, H O, Bostian, K A
We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in...
Tait-Kamradt, A G, Turner, K J, Kramer, R A, Elliott, Q D, Bostian, S J, Thill, G P, ...
We characterized the organization and expression of PHO5 and PHO3, the tightly linked repressible and constitutive acid phosphatase genes of Saccharomyces cerevisiae. The "constitutive" gene, PHO3,...
Thill, G P, Kramer, R A, Turner, K J, Bostian, K A
The nucleotide sequence of 5'-noncoding and N-terminal coding regions of two coordinately regulated, repressible acid phosphatase genes from Saccharomyces cerevisiae were determined. These unlinked...
Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.
Bostian, K A, Lemire, J M, Halvorson, H O
We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum], EC 3.1.3.2) in Saccharomyces cerevisiae at the physiological and...
El-Sherbeini, M, Tipper, D J, Mitchell, D J, Bostian, K A
The plasmid determinants of killer phenotypes in type K1 and K2 killer yeast cells are the 1.9-kilobase (kb) M1 and 1.7-kb M2 double-stranded RNAs (dsRNAs), respectively. These are dependent for...
Bostian, K A, Lemire, J M, Halvorson, H O
The synthesis of repressible acid phosphatase in Saccharomyces cerevisiae was examined under conditions of blocked derepression as described by Toh-e et al. (Mol. Gen. Genet. 162:139-149, 1978)....
Secretion of Saccharomyces cerevisiae Killer Toxin: Processing of the Glycosylated Precursor
Bussey, H., Saville, D., Greene, D., Tipper, D. J., Bostian, K. A.
Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of...
Effect of Newcastle Disease on Serum Copper, Zinc, Cholesterol, and Carotenoid Values in the Chick
Squibb, R. L., Beisel, W. R., Bostian, K. A.
Experimentally induced Newcastle disease virus infection of chicks, with a mortality index of 48%, was accompanied by increased concentrations in serum of copper and cholesterol and decreased...
Canonico, P G, Little, J S, Powanda, M C, Bostian, K A, Beisel, W R
Streptococcus pneumoniae infection leads to multifold increases in sialyltransferase, galactosyltransferase, alpha 2-fucosyltransferase, and alpha 3-fucosyltransferase activity of rat liver. Such...
Bostian, K A, Betts, G F, Man, W K, Hughes, M N
Univalent cation activators of aldehyde dehydrogenase have dual effects, both interpreted as cation-induced or -stabilized conformation changes. These two processes are differentiated by the time...
Betts, G F, Poole, P L, Springham, M G, Bostian, K A
The activity, stability and spectroscopic properties of yeast K+ -activated aldehyde dehydrogenase were measured at various times after removal from, and after returning to a solution containing K+....
Mapping of functional domains within the Saccharomyces cerevisiae type 1 killer preprotoxin.
Sturley, S L, Elliot, Q, LeVitre, J, Tipper, D J, Bostian, K A
Strains of Saccharomyces cerevisiae harboring M1-dsRNA, the determinant of type 1 killer and immunity phenotypes, secrete a dimeric 19-kd toxin that kills sensitive yeast cells by the production of...
A method for the purification of yeast K+-activated aldehyde dehydrogenase is presented which can be completed in substantially less time than other published procedures. The enzyme has a different...
Data from steady-state kinetic analysis of yeast K+-activated aldehyde dehydrogenase are consistent with a ternary complex mechanism. Evidence from alternative substrate analysis and...