Drusano, G. L., Preston, S. L., Smee, D., Bush, K., Bailey, K., Sidwell, R. W.
We examined RWJ-270201 in a lethal model of influenza in BALB/c mice. The aim was to delineate the pharmacodynamically linked variable for the drug. Challenge was performed with influenza virus...
Bradford, P A, Urban, C, Jaiswal, A, Mariano, N, Rasmussen, B A, Projan, S J, ...
Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be...
A functional classification scheme for beta-lactamases and its correlation with molecular structure.
Bradford, P A, Jacobus, N V, Bhachech, N, Bush, K
TEM-28 (pI 6.1), expressed by an Escherichia coli clinical isolate, is a novel beta-lactamase which hydrolyzed ceftazidime, cefotaxime, and aztreonam with rates of 25, 1.1, and 5.6, respectively,...
Rasmussen, B A, Bush, K, Keeney, D, Yang, Y, Hare, R, O'Gara, C, ...
In 1984, a year prior to the U.S. approval of imipenem for clinical use, a wound isolate and a bile isolate of Enterobacter cloacae were obtained from two patients in a California hospital. These...
Bradford, P A, Urban, C, Mariano, N, Projan, S J, Rahal, J J, Bush, K
Six Escherichia coli and 12 Klebsiella pneumoniae isolates from a single hospital expressed a common beta-lactamase with a pI of approximately 9.0 and were resistant to cefoxitin and cefotetan (MIC...
Antibacterial activity of BMS-180680, a new catechol-containing monobactam.
Fung-Tomc, J, Bush, K, Minassian, B, Kolek, B, Flamm, R, Gradelski, E, ...
The in vitro activities of a new catechol-containing monobactam, BMS-180680 (SQ 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin,...
Penicillin-binding proteins and induction of AmpC beta-lactamase.
Sanders, C C, Bradford, P A, Ehrhardt, A F, Bush, K, Young, K D, Henderson, T A, ...
In competition assays for radiolabeled penicillin, penicillin-binding proteins (PBPs) 4, 7a, and 7b showed very high affinities for strong inducers of AmpC beta-lactamase. Loss of PBP 4 resulted in...
Quinn, J P, Miyashiro, D, Sahm, D, Flamm, R, Bush, K
Two clinical isolates of Klebsiella pneumoniae from seriously ill patients in Chicago, Ill., have been identified as resistant to ceftazidime and aztreonam but susceptible to other cephalosporins....
In vitro evaluation of tigemonam, a novel oral monobactam.
Tanaka, S K, Summerill, R A, Minassian, B F, Bush, K, Visnic, D A, Bonner, D P, ...
Tigemonam, a novel, orally administered monobactam, exhibited potent and specific activity in vitro against members of the family Enterobacteriaceae, Haemophilus influenzae, and Neisseria...
Bush, K, Smith, S A, Ohringer, S, Tanaka, S K, Bonner, D P
Tigemonam and temocillin, but not aztreonam, bound to penicillin-binding proteins (PBPs) 1a and 3 of Escherichia coli with apparent improved affinity when challenged with benzylpenicillin at lowered...
Resistance caused by decreased penetration of beta-lactam antibiotics into Enterobacter cloacae.
Bush, K, Tanaka, S K, Bonner, D P, Sykes, R B
Strains of Enterobacter cloacae were selected on the basis of resistance to aztreonam, ceftazidime, moxalactam, or imipenem. All strains produced the same E2 beta-lactamase, with an isoelectric point...
Sykes, R B, Bonner, D P, Bush, K, Georgopapadakou, N H
Azthreonam (SQ 26,776) is a synthetic monocyclic beta-lactam antimicrobial agent belonging to the monobactam family (Sykes et al., Nature [London] 291:489-491, 1981), members of which are...
Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
Bush, K, Freudenberger, J S, Sykes, R B
Monobactams containing 3 beta-aminothiazolyl oxime side chains (SQ 81,377, SQ 81,402, azthreonam, and SQ 26,917) have poor affinities for the broad-spectrum beta-lactamases TEM-2 and K1. Addition of...
Kinetic interactions of tazobactam with beta-lactamases from all major structural classes.
Bush, K, Macalintal, C, Rasmussen, B A, Lee, V J, Yang, Y
Tazobactam was shown to be a potent inhibitor of group 1, 2a, 2b, and 2b' beta-lactamases. Extended kinetic studies with class A and C serine beta-lactamases showed that the PC1, TEM-2, and P99...
Rasmussen, B A, Bradford, P A, Quinn, J P, Wiener, J, Weinstein, R A, Bush, K
Ceftazidime-resistant isolates of Escherichia coli and Klebsiella pneumoniae produced a plasmid-mediated beta-lactamase with a pI of 5.6 with biochemical characteristics comparable to those of the...
Biochemical characterization of the metallo-beta-lactamase CcrA from Bacteroides fragilis TAL3636.
Yang, Y, Rasmussen, B A, Bush, K
The CcrA beta-lactamase from Bacteroides fragilis TAL3636 was cloned into Escherichia coli and purified from inclusion bodies. This group 3 metalloenzyme hydrolyzed most beta-lactam antibiotics,...
Naumovski, L, Quinn, J P, Miyashiro, D, Patel, M, Bush, K, Singer, S B, ...
Ceftazidime is widely used in the therapy of infectious complications in neutropenic patients. We studied an outbreak of ceftazidime-resistant gram-negative bacillary infections in pediatric cancer...
Bush, K, Flamm, R K, Ohringer, S, Singer, S B, Summerill, R, Bonner, D P
An isolate of Serratia marcescens that produced both an inducible chromosomal and a plasmid-mediated TEM-1 beta-lactamase was resistant to ampicillin and amoxicillin and also demonstrated decreased...
Urban, C, Meyer, K S, Mariano, N, Rahal, J J, Flamm, R, Rasmussen, B A, ...
An epidemic of nosocomial ceftazidime-resistant Klebsiella pneumoniae was correlated with production of a ceftazidime-hydrolyzing enzyme with an isoelectric point of 5.6 (BMH-1). BMH-1 was encoded on...
Bradford, P A, Cherubin, C E, Idemyor, V, Rasmussen, B A, Bush, K
Ceftazidime-resistant Klebsiella pneumoniae strains began to appear when ceftazidime usage was increased in two unrelated Chicago hospitals. These strains produced a beta-lactamase with an...
Rasmussen, B A, Keeney, D, Yang, Y, Bush, K
Two beta-lactamase genes, asbA1 and asbB1, encoding AsbA1 and AsbB1, respectively, have been cloned from Aeromonas sobria AER 14M into Escherichia coli. AsbA1 was expressed at low but detectable...
Rasmussen, B A, Yang, Y, Jacobus, N, Bush, K
The metallo-beta-lactamase gene, ccrA, has been cloned from three clinical isolates of Bacteroides fragilis, TAL3636, QMCN3, and QMCN4. Although all three isolates harbored a gene encoding a potent...
Gonzalez Leiza, M, Perez-Diaz, J C, Ayala, J, Casellas, J M, Martinez-Beltran, J, Bush, K, ...
Klebsiella pneumoniae BA32, a clinical isolate from Buenos Aires, Argentina, was found to produce a plasmid-encoded beta-lactamase (FOX-1) which conferred resistance to broad-spectrum cephalosporins...
Beta-lactamase inhibitors from laboratory to clinic.
beta-Lactamases constitute the major defense mechanism of pathogenic bacteria against beta-lactam antibiotics. When the beta-lactam ring of this antibiotic class is hydrolyzed, antimicrobial activity...
Properties of a class C beta-lactamase from Serratia marcescens.
Joris, B, De Meester, F, Galleni, M, Masson, S, Dusart, J, Frère, J M, ...
A beta-lactamase produced by a penicillin-resistant strain of Serratia marcescens was isolated and purified. The kcat. value for benzylpenicillin was about 5% of that observed for the best...
Lolans, K., Queenan, A. M., Bush, K., Sahud, A., Quinn, J. P.
Carbapenemases are rare in the United States. This is the first report of a United States nosocomial outbreak of pan-resistant Pseudomonas aeruginosa infections due to an integron-borne...
Drusano, G. L., Preston, S. L., Smee, D., Bush, K., Bailey, K., Sidwell, R. W.
We examined RWJ-270201 in a lethal model of influenza in BALB/c mice. The aim was to delineate the pharmacodynamically linked variable for the drug. Challenge was performed with influenza virus...
Bradford, P A, Urban, C, Jaiswal, A, Mariano, N, Rasmussen, B A, Projan, S J, ...
Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be...
A functional classification scheme for beta-lactamases and its correlation with molecular structure.
Bradford, P A, Jacobus, N V, Bhachech, N, Bush, K
TEM-28 (pI 6.1), expressed by an Escherichia coli clinical isolate, is a novel beta-lactamase which hydrolyzed ceftazidime, cefotaxime, and aztreonam with rates of 25, 1.1, and 5.6, respectively,...
Rasmussen, B A, Bush, K, Keeney, D, Yang, Y, Hare, R, O'Gara, C, ...
In 1984, a year prior to the U.S. approval of imipenem for clinical use, a wound isolate and a bile isolate of Enterobacter cloacae were obtained from two patients in a California hospital. These...
Bradford, P A, Urban, C, Mariano, N, Projan, S J, Rahal, J J, Bush, K
Six Escherichia coli and 12 Klebsiella pneumoniae isolates from a single hospital expressed a common beta-lactamase with a pI of approximately 9.0 and were resistant to cefoxitin and cefotetan (MIC...
Antibacterial activity of BMS-180680, a new catechol-containing monobactam.
Fung-Tomc, J, Bush, K, Minassian, B, Kolek, B, Flamm, R, Gradelski, E, ...
The in vitro activities of a new catechol-containing monobactam, BMS-180680 (SQ 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin,...
Penicillin-binding proteins and induction of AmpC beta-lactamase.
Sanders, C C, Bradford, P A, Ehrhardt, A F, Bush, K, Young, K D, Henderson, T A, ...
In competition assays for radiolabeled penicillin, penicillin-binding proteins (PBPs) 4, 7a, and 7b showed very high affinities for strong inducers of AmpC beta-lactamase. Loss of PBP 4 resulted in...
Quinn, J P, Miyashiro, D, Sahm, D, Flamm, R, Bush, K
Two clinical isolates of Klebsiella pneumoniae from seriously ill patients in Chicago, Ill., have been identified as resistant to ceftazidime and aztreonam but susceptible to other cephalosporins....
In vitro evaluation of tigemonam, a novel oral monobactam.
Tanaka, S K, Summerill, R A, Minassian, B F, Bush, K, Visnic, D A, Bonner, D P, ...
Tigemonam, a novel, orally administered monobactam, exhibited potent and specific activity in vitro against members of the family Enterobacteriaceae, Haemophilus influenzae, and Neisseria...
Bush, K, Smith, S A, Ohringer, S, Tanaka, S K, Bonner, D P
Tigemonam and temocillin, but not aztreonam, bound to penicillin-binding proteins (PBPs) 1a and 3 of Escherichia coli with apparent improved affinity when challenged with benzylpenicillin at lowered...
Resistance caused by decreased penetration of beta-lactam antibiotics into Enterobacter cloacae.
Bush, K, Tanaka, S K, Bonner, D P, Sykes, R B
Strains of Enterobacter cloacae were selected on the basis of resistance to aztreonam, ceftazidime, moxalactam, or imipenem. All strains produced the same E2 beta-lactamase, with an isoelectric point...
Sykes, R B, Bonner, D P, Bush, K, Georgopapadakou, N H
Azthreonam (SQ 26,776) is a synthetic monocyclic beta-lactam antimicrobial agent belonging to the monobactam family (Sykes et al., Nature [London] 291:489-491, 1981), members of which are...
Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
Bush, K, Freudenberger, J S, Sykes, R B
Monobactams containing 3 beta-aminothiazolyl oxime side chains (SQ 81,377, SQ 81,402, azthreonam, and SQ 26,917) have poor affinities for the broad-spectrum beta-lactamases TEM-2 and K1. Addition of...
Kinetic interactions of tazobactam with beta-lactamases from all major structural classes.
Bush, K, Macalintal, C, Rasmussen, B A, Lee, V J, Yang, Y
Tazobactam was shown to be a potent inhibitor of group 1, 2a, 2b, and 2b' beta-lactamases. Extended kinetic studies with class A and C serine beta-lactamases showed that the PC1, TEM-2, and P99...
Rasmussen, B A, Bradford, P A, Quinn, J P, Wiener, J, Weinstein, R A, Bush, K
Ceftazidime-resistant isolates of Escherichia coli and Klebsiella pneumoniae produced a plasmid-mediated beta-lactamase with a pI of 5.6 with biochemical characteristics comparable to those of the...
Biochemical characterization of the metallo-beta-lactamase CcrA from Bacteroides fragilis TAL3636.
Yang, Y, Rasmussen, B A, Bush, K
The CcrA beta-lactamase from Bacteroides fragilis TAL3636 was cloned into Escherichia coli and purified from inclusion bodies. This group 3 metalloenzyme hydrolyzed most beta-lactam antibiotics,...
Naumovski, L, Quinn, J P, Miyashiro, D, Patel, M, Bush, K, Singer, S B, ...
Ceftazidime is widely used in the therapy of infectious complications in neutropenic patients. We studied an outbreak of ceftazidime-resistant gram-negative bacillary infections in pediatric cancer...
Bush, K, Flamm, R K, Ohringer, S, Singer, S B, Summerill, R, Bonner, D P
An isolate of Serratia marcescens that produced both an inducible chromosomal and a plasmid-mediated TEM-1 beta-lactamase was resistant to ampicillin and amoxicillin and also demonstrated decreased...
Urban, C, Meyer, K S, Mariano, N, Rahal, J J, Flamm, R, Rasmussen, B A, ...
An epidemic of nosocomial ceftazidime-resistant Klebsiella pneumoniae was correlated with production of a ceftazidime-hydrolyzing enzyme with an isoelectric point of 5.6 (BMH-1). BMH-1 was encoded on...
Bradford, P A, Cherubin, C E, Idemyor, V, Rasmussen, B A, Bush, K
Ceftazidime-resistant Klebsiella pneumoniae strains began to appear when ceftazidime usage was increased in two unrelated Chicago hospitals. These strains produced a beta-lactamase with an...
Rasmussen, B A, Keeney, D, Yang, Y, Bush, K
Two beta-lactamase genes, asbA1 and asbB1, encoding AsbA1 and AsbB1, respectively, have been cloned from Aeromonas sobria AER 14M into Escherichia coli. AsbA1 was expressed at low but detectable...
Rasmussen, B A, Yang, Y, Jacobus, N, Bush, K
The metallo-beta-lactamase gene, ccrA, has been cloned from three clinical isolates of Bacteroides fragilis, TAL3636, QMCN3, and QMCN4. Although all three isolates harbored a gene encoding a potent...
Gonzalez Leiza, M, Perez-Diaz, J C, Ayala, J, Casellas, J M, Martinez-Beltran, J, Bush, K, ...
Klebsiella pneumoniae BA32, a clinical isolate from Buenos Aires, Argentina, was found to produce a plasmid-encoded beta-lactamase (FOX-1) which conferred resistance to broad-spectrum cephalosporins...
Beta-lactamase inhibitors from laboratory to clinic.
beta-Lactamases constitute the major defense mechanism of pathogenic bacteria against beta-lactam antibiotics. When the beta-lactam ring of this antibiotic class is hydrolyzed, antimicrobial activity...
Properties of a class C beta-lactamase from Serratia marcescens.
Joris, B, De Meester, F, Galleni, M, Masson, S, Dusart, J, Frère, J M, ...
A beta-lactamase produced by a penicillin-resistant strain of Serratia marcescens was isolated and purified. The kcat. value for benzylpenicillin was about 5% of that observed for the best...
Lolans, K., Queenan, A. M., Bush, K., Sahud, A., Quinn, J. P.
Carbapenemases are rare in the United States. This is the first report of a United States nosocomial outbreak of pan-resistant Pseudomonas aeruginosa infections due to an integron-borne...
RWJ-270201 (BCX-1812): a novel neuraminidase inhibitor for influenza.
The influenza virus neuraminidase (NA) is important in the pathogenesis of infection and, thus, is an attractive target for agents used in the treatment and prophylaxis of influenza. This article...
Concha, N. O., Rasmussen, B. A., Bush, K., Herzberg, O.
The metallo-beta-lactamases require zinc or cadmium for hydrolyzing beta-lactam antibiotics and are inhibited by mercurial compounds. To data, there are no clinically useful inhibitors of this class...