K. G. Murti

Telomeres of polytene chromosomes in a ciliated protozoan terminate in duplex DNA loops

Murti, K. G., Prescott, D. M.

The end of a telomeric DNA sequence isolated from a polytene chromosome of a hypotrichous ciliate folds back and hybridizes with downstream telomeric sequence to form a t loop that is stable in the...

Crystals of hemagglutinin-neuraminidase of parainfluenza virus contain triple-stranded helices.

Murti, K G, Takimoto, T, Laver, W G, Portner, A

When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle...

Highly localized tracks of human immunodeficiency virus type 1 Nef in the nucleus of cells of a human CD4+ T-cell line.

Murti, K G, Brown, P S, Ratner, L, Garcia, J V

A human T-cell line constitutively expressing the nef gene from the human immunodeficiency virus type 1 SF2 isolate was used to examine the distribution of the Nef protein in the nucleus....

Secondary structural features in the 70S RNAs of Moloney murine leukemia and Rous sarcoma viruses as observed by electron microscopy.

Murti, K G, Bondurant, M, Tereba, A

The secondary structural features in the 70S RNAs of the Prague strain of avian Rous sarcoma virus, subgroup A (PR-RSV-A), and Moloney murine leukemia virus (M-MuLV) were compared by electron...

The cytoplasmic tail of influenza A virus neuraminidase (NA) affects NA incorporation into virions, virion morphology, and virulence in mice but is not essential for virus replication.

Mitnaul, L J, Castrucci, M R, Murti, K G, Kawaoka, Y

In this study, we investigated the role of the conserved neuraminidase (NA) cytoplasmic tail residues in influenza virus replication. Mutants of influenza A virus (A/WSN/33 [H1N1]) with deletions of...

Characterization of mutants of influenza A virus selected with the neuraminidase inhibitor 4-guanidino-Neu5Ac2en.

Gubareva, L V, Bethell, R, Hart, G J, Murti, K G, Penn, C R, Webster, R G

The development of viral resistance to the neuraminidase (NA) inhibitor, 4-guanidino-Neu5Ac2en, of influenza viruses was studied by serial passage of A/Turkey/Minnesota/833/80 (H4N2) in Madin-Darby...

Divergence between cytotoxic effector function and tumor necrosis factor alpha production for inflammatory CD4+ T cells from mice with Sendai virus pneumonia.

Hou, S, Fishman, M, Murti, K G, Doherty, P C

Sendai virus pneumonia in beta 2-microglobulin-deficient [beta 2-m(-/-)] mice lacking CD8+ T cells is characterized by the development of CD4+ cytotoxic T lymphocytes that can be recovered directly...

Crystallization of biologically active hemagglutinin-neuraminidase glycoprotein dimers proteolytically cleaved from human parainfluenza virus type 1.

Takimoto, T, Laver, W G, Murti, K G, Portner, A

We isolated, purified, and characterized the hemagglutinin-neuraminidase (HN) of human parainfluenza virus type 1, with the ultimate goal of producing crystals suitable for three-dimensional X-ray...

Isolation of a biologically active soluble form of the hemagglutinin-neuraminidase protein of Sendai virus.

Thompson, S D, Laver, W G, Murti, K G, Portner, A

As a first step in establishing the three-dimensional structure of the Sendai virus hemagglutinin-neuraminidase (HN), we have isolated and characterized a potentially crystallizable form of the...

Ubiquitin is a component of the microtubule network.

Murti, K G, Smith, H T, Fried, V A

Immunofluorescence microscopy was used to study the intracellular localization of ubiquitin. Baby hamster kidney cells (BHK cells) and several other cell lines were probed with a well characterized...

In vivo expression of mammalian BiP ATPase mutants causes disruption of the endoplasmic reticulum.

Hendershot, L M, Wei, J Y, Gaut, J R, Lawson, B, Freiden, P J, Murti, K G

BiP possesses ATP binding/hydrolysis activities that are thought to be essential for its ability to chaperone protein folding and assembly in the endoplasmic reticulum (ER). We have produced a series...

The genome of frog virus 3, an animal DNA virus, is circularly permuted and terminally redundant.

Goorha, R, Murti, K G

We examined the structure of the frog virus 3 (FV 3) genome by using electron microscopic and biochemical techniques. The linear FV 3 DNA molecules (Mr approximately 100 x 10(6) formed circles when...

Assembly of vesicular stomatitis virus nucleocapsids in vivo: a kinetic analysis.

Hsu, C H, Kingsbury, D W, Murti, K G

Pulse-chase labeling and cell fractionation were used to examine the pathways taken by the three nucleocapsid polypeptide species of vesicular stomatitis virus into nucleocapsids and then into...

Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.

Murti, K G, Prescott, D M

Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous...

Chromosome 1 contains the endogenous RAV-0 retrovirus sequences in chicken cells.

Tereba, A, Lai, M M, Murti, K G

We have developed a structurally unique probe which can be used to determine the chromosomal location of nonreiterated genes in vertebrate organisms by the method of in situ hybridization. The probe...

The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.

Fornerod, M, Van Deursen, J, Van Baal, S, Reynolds, A, Davis, D, Murti, K G, ...

The oncogenic nucleoporin CAN/Nup214 is essential in vertebrate cells. Its depletion results in defective nuclear protein import, inhibition of messenger RNA export and cell cycle arrest. We recently...

Telomeres of polytene chromosomes in a ciliated protozoan terminate in duplex DNA loops

Murti, K. G., Prescott, D. M.

The end of a telomeric DNA sequence isolated from a polytene chromosome of a hypotrichous ciliate folds back and hybridizes with downstream telomeric sequence to form a t loop that is stable in the...

Crystals of hemagglutinin-neuraminidase of parainfluenza virus contain triple-stranded helices.

Murti, K G, Takimoto, T, Laver, W G, Portner, A

When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle...

Highly localized tracks of human immunodeficiency virus type 1 Nef in the nucleus of cells of a human CD4+ T-cell line.

Murti, K G, Brown, P S, Ratner, L, Garcia, J V

A human T-cell line constitutively expressing the nef gene from the human immunodeficiency virus type 1 SF2 isolate was used to examine the distribution of the Nef protein in the nucleus....

Secondary structural features in the 70S RNAs of Moloney murine leukemia and Rous sarcoma viruses as observed by electron microscopy.

Murti, K G, Bondurant, M, Tereba, A

The secondary structural features in the 70S RNAs of the Prague strain of avian Rous sarcoma virus, subgroup A (PR-RSV-A), and Moloney murine leukemia virus (M-MuLV) were compared by electron...

The cytoplasmic tail of influenza A virus neuraminidase (NA) affects NA incorporation into virions, virion morphology, and virulence in mice but is not essential for virus replication.

Mitnaul, L J, Castrucci, M R, Murti, K G, Kawaoka, Y

In this study, we investigated the role of the conserved neuraminidase (NA) cytoplasmic tail residues in influenza virus replication. Mutants of influenza A virus (A/WSN/33 [H1N1]) with deletions of...

Characterization of mutants of influenza A virus selected with the neuraminidase inhibitor 4-guanidino-Neu5Ac2en.

Gubareva, L V, Bethell, R, Hart, G J, Murti, K G, Penn, C R, Webster, R G

The development of viral resistance to the neuraminidase (NA) inhibitor, 4-guanidino-Neu5Ac2en, of influenza viruses was studied by serial passage of A/Turkey/Minnesota/833/80 (H4N2) in Madin-Darby...

Divergence between cytotoxic effector function and tumor necrosis factor alpha production for inflammatory CD4+ T cells from mice with Sendai virus pneumonia.

Hou, S, Fishman, M, Murti, K G, Doherty, P C

Sendai virus pneumonia in beta 2-microglobulin-deficient [beta 2-m(-/-)] mice lacking CD8+ T cells is characterized by the development of CD4+ cytotoxic T lymphocytes that can be recovered directly...

Crystallization of biologically active hemagglutinin-neuraminidase glycoprotein dimers proteolytically cleaved from human parainfluenza virus type 1.

Takimoto, T, Laver, W G, Murti, K G, Portner, A

We isolated, purified, and characterized the hemagglutinin-neuraminidase (HN) of human parainfluenza virus type 1, with the ultimate goal of producing crystals suitable for three-dimensional X-ray...

Isolation of a biologically active soluble form of the hemagglutinin-neuraminidase protein of Sendai virus.

Thompson, S D, Laver, W G, Murti, K G, Portner, A

As a first step in establishing the three-dimensional structure of the Sendai virus hemagglutinin-neuraminidase (HN), we have isolated and characterized a potentially crystallizable form of the...

Ubiquitin is a component of the microtubule network.

Murti, K G, Smith, H T, Fried, V A

Immunofluorescence microscopy was used to study the intracellular localization of ubiquitin. Baby hamster kidney cells (BHK cells) and several other cell lines were probed with a well characterized...

In vivo expression of mammalian BiP ATPase mutants causes disruption of the endoplasmic reticulum.

Hendershot, L M, Wei, J Y, Gaut, J R, Lawson, B, Freiden, P J, Murti, K G

BiP possesses ATP binding/hydrolysis activities that are thought to be essential for its ability to chaperone protein folding and assembly in the endoplasmic reticulum (ER). We have produced a series...

The genome of frog virus 3, an animal DNA virus, is circularly permuted and terminally redundant.

Goorha, R, Murti, K G

We examined the structure of the frog virus 3 (FV 3) genome by using electron microscopic and biochemical techniques. The linear FV 3 DNA molecules (Mr approximately 100 x 10(6) formed circles when...

Assembly of vesicular stomatitis virus nucleocapsids in vivo: a kinetic analysis.

Hsu, C H, Kingsbury, D W, Murti, K G

Pulse-chase labeling and cell fractionation were used to examine the pathways taken by the three nucleocapsid polypeptide species of vesicular stomatitis virus into nucleocapsids and then into...

Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.

Murti, K G, Prescott, D M

Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous...

Chromosome 1 contains the endogenous RAV-0 retrovirus sequences in chicken cells.

Tereba, A, Lai, M M, Murti, K G

We have developed a structurally unique probe which can be used to determine the chromosomal location of nonreiterated genes in vertebrate organisms by the method of in situ hybridization. The probe...

The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.

Fornerod, M, Van Deursen, J, Van Baal, S, Reynolds, A, Davis, D, Murti, K G, ...

The oncogenic nucleoporin CAN/Nup214 is essential in vertebrate cells. Its depletion results in defective nuclear protein import, inhibition of messenger RNA export and cell cycle arrest. We recently...