Puschendorf, M., Terranova, R., Boutsma, E., Mao, X., Isono, K., Brykczynska, U., ...
In eukaryotes, Suv39h H3K9 trimethyltransferases are required for pericentric heterochromatin formation and function. In early mouse preimplantation embryos, however, paternal pericentric...
Control of developmental regulators by Polycomb in human embryonic stem cells. (2006)
Lee, T.I., Jenner, R.G., Boyer, L.A., Guenther, M.G., Levine, S.S., Kumar, R.M., ...
Control of developmental regulators by Polycomb in human embryonic stem cells. (2006)
Lee, T.I., Jenner, R.G., Boyer, L.A., Guenther, M.G., Levine, S.S., Kumar, R.M., ...
Hanaoka, T, Tsugane, S, Ando, N, Ishida, K, Kakegawa, T, Isono, K, ...
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction (1994)
J. Tamaoki, A. Chiyotani, E. Tagaya, K. Isono, K. Konno
To elucidate the modulatory role of histamine-degrading enzymes in airway constrictor responses, human bronchial strips were studied under isometric conditions in vitro. Pretreatment of tissues with...
Kasai, H., Isono, S., Kitakawa, M., Mineno, J., Akiyama, H., Kurnit, D.M., ...
We have developed a strategy for efficient sequence analysis of the genome of E.coli K-12 using insertions of a Tn5-derived mini-transposon into overlapping ordered λ phage clones to provide...
Isono, K, Yamamoto, H, Satoh, K, Kobayashi, H
A cDNA encoding a putative RNA and/or DNA helicase has been isolated from Arabidopsis thaliana cDNA libraries. The cloned cDNA is 5166 bases long, and its largest open reading frame encodes 1538...
Isono, K, Niwa, Y, Satoh, K, Kobayashi, H
Mechanisms underlying suppressed levels of transcripts for plastid photosynthesis genes in nongreen tissues such as roots and calli were analyzed in Arabidopsis thaliana, a plant suitable for further...
Mechanism of action and selective toxicity of ascamycin, a nucleoside antibiotic.
An unidentified Streptomyces sp. produces two nucleoside antibiotics, ascamycin and its dealanyl derivative. In contrast to the broad antibacterial activity of dealanylascamycin against various...
An ordered clone bank for chromosome I of Saccharomyces cerevisiae.
Tanaka, S, Yoshikawa, A, Isono, K
Chromosome I of Saccharomyces cerevisiae DC5 rho 0 was dissected into segments with an average size of 14.0 kb and cloned into lambda phage vectors. The physical maps of the resultant clones,...
Kang, W, Matsushita, Y, Grohmann, L, Graack, H R, Kitakawa, M, Isono, K
The N-terminal amino acid sequence of a large subunit protein, termed YmL33, of the mitochondrial ribosome of the yeast Saccharomyces cerevisiae was determined. The data were obtained to synthesize...
Tanaka, Y, Tsujimura, A, Fujita, N, Isono, S, Isono, K
The chromosomal DNA fragments of Escherichia coli K-12 were cloned into a mini-F cosmid, pRE435, after partial digestion with restriction endonuclease Sau3AI. The clones were first screened for PyrC+...
Satoh, H, Nishida, H, Isono, K
The gene for an alpha-amylase cloned from strain DY-5 of Bacillus stearothermophilus was used to examine to what extent the corresponding genes are structurally similar in other B. stearothermophilus...
Location of a gene (ssrA) for a small, stable RNA (10Sa RNA) in the Escherichia coli chromosome.
Oh, B K, Chauhan, A K, Isono, K, Apirion, D
The gene for 10Sa RNA, which is a major small, stable RNA in Escherichia coli, is a unique gene in the E. coli chromosome. The 10Sa RNA gene (ssrA) has been located between 2,760 and 2,761 kilobases...
Lipopolysaccharide from Klebsiella pneumoniae inhibits Na+ absorption in canine tracheal epithelium.
Tamaoki, J, Sakai, N, Isono, K, Kanemura, T, Takeyama, K, Takizawa, T
The effect of lipopolysaccharide (LPS) from Klebsiella pneumoniae on the bioelectric properties of canine cultured tracheal epithelium was examined. LPS decreased short-circuit current (Isc), and its...
Gene encoding cytoskeletal proteins in Drosophila rhabdomeres.
Matsumoto, H, Isono, K, Pye, Q, Pak, W L
The ninaC gene is one of eight nina (neither inactivation nor afterpotential) genes identified from mutations that drastically reduce the amount of rhodopsin in the compound eye of Drosophila...
During the course of systematic nucleotide sequence analysis of the terC region of E.coli K-12 by using the ordered lambda phage clones, we found the presence of a gene, termed hrpA, that showed a...
Most of the 155 transcripts of the genes on chromosome III of S.cerevisiae that were identified by a series of Northern hybridization experiments (Yoshikawa, A. and Isono, K., Yeast 6, 383-401, 1990)...
The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1.
The DNA sequence of the gene rpsA as well as of its neighboring regions has been determined using the dideoxyribonucleotide method. It was found that there is an "open-reading-frame" of 350 bp which...
A computer program package for storing and retrieving DNA/RNA and protein sequence data.
A computer program package has been made available which contains five compound programs written in FORTRAN 10 (for DECsystem-10). Program DATBAS is for storing and improving DNA sequence data,...
Computer programs to analyze DNA and amino acid sequence data.
Extensive modifications have been incorporated into many of the computer programs written by Staden (1-4) to make them easier to cope with DNA and amino acid sequence data. These programs can be...
The SSP1 gene of Schizosaccharomyces pombe which is homologous to the SSC1 gene of Saccharomyces cerevisiae was cloned and its nucleotide sequence determined. A heat-shock element and three possible...
Kitakawa, M, Grohmann, L, Graack, H R, Isono, K
The genes for two large subunit proteins, YmL8 and YmL20, of the mitochondrial ribosome of Saccharomyces cerevisiae were cloned by hybridization with synthetic oligonucleotide mixtures corresponding...
By comparing sequences of restriction enzyme cleavage sites and their distance data, we sorted 384 lambda phage clones containing segments of chromosome VI of S. cerevisiae and constructed an ordered...
Kasai, H, Isono, S, Kitakawa, M, Mineno, J, Akiyama, H, Kurnit, D M, ...
We have developed a strategy for efficient sequence analysis of the genome of E. coli K-12 using insertions of a Tn5-derived mini-transposon into overlapping ordered lambda phage clones to provide...
Lack of ribosomal protein S1 in Bacillus stearothermophilus.
The 30S ribosomal subunit of Bacillus stearothermophilus migrated as a single band when electrophoresed on agarose-acrylamide composite gels. The addition of the ribosomal protein S1 purified from...
Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA.
Schnier, J, Kimura, M, Foulaki, K, Subramanian, A R, Isono, K, Wittmann-Liebold, B
The primary structure of proteins S1, the largest protein component of the Escherichia coli ribosome, has been elucidated by determining the amino acid sequence of the protein (from E. coli MRE600)...
A mutant of Escherichia coli K-12 isolated for temperature-sensitive growth was found to harbor an alteration in robosomal protein L9. Because the chromosomal location of the structural gene for this...
Kanno, M, Hasegawa, M, Ishida, A, Isono, K, Taniguchi, M
The mammalian mel-18/bmi-1 gene products share an amino acid sequence and a secondary structure, including a RING-finger motif, with the Drosophila Polycomb group (PcG) gene products Psc and Su(z)2,...
Takai, A, Sasaki, K, Nagai, H, Mieskes, G, Isobe, M, Isono, K, ...
Several groups have reported that okadaic acid (OA) and some other tight-binding protein phosphatase inhibitors including microcystin-LR (MCLR), calyculin-A and tautomycin prevent each other from...
Purification and characterization of ascamycin-hydrolysing aminopeptidase from Xanthomonas citri.
A nucleoside antibiotic, ascamycin (9-beta-[5'-0-(N-L-alanyl) sulphamoyl-D-ribofuranosyl]-2-chloroadenine), has a selective antibacterial activity against Xanthomonas species. When ascamycin was...
Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.
Kitakawa, M, Dabbs, E R, Isono, K
Mutants with alterations in the structural genes for ribosomal proteins S15, L21, and L27 were used in mapping the genes coding for these proteins. Results from P1kc-mediated transductions indicate...
Physical dissection and characterization of chromosomes V and VIII of Saccharomyces cerevisiae.
Chromosomes V and VIII of S. cerevisiae were dissected and ordered clone banks were constructed and characterized. Each bank contains almost the entire chromosome from the left to the right telomere...
Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.
Yura, T, Mori, H, Nagai, H, Nagata, T, Ishihama, A, Fujita, N, ...
A contiguous 111,402-nucleotide sequence corresponding to the 0 to 2.4 min region of the E. coli chromosome was determined as a first step to complete structural analysis of the genome. The resulting...
Taste Sensitivity to Trehalose and Its Alteration by Gene Dosage in Drosophila Melanogaster
Tanimura, T., Isono, K., Yamamoto, M. T.
The taste sensitivity to the disaccharide trehalose of Drosophila melanogaster is under the genetic control by the Tre gene on the X chromosome. The gene is genetically dimorphic for high and low...
Isono, K, Yamamoto, H, Satoh, K, Kobayashi, H
A cDNA encoding a putative RNA and/or DNA helicase has been isolated from Arabidopsis thaliana cDNA libraries. The cloned cDNA is 5166 bases long, and its largest open reading frame encodes 1538...
Isono, K, Niwa, Y, Satoh, K, Kobayashi, H
Mechanisms underlying suppressed levels of transcripts for plastid photosynthesis genes in nongreen tissues such as roots and calli were analyzed in Arabidopsis thaliana, a plant suitable for further...
Mechanism of action and selective toxicity of ascamycin, a nucleoside antibiotic.
An unidentified Streptomyces sp. produces two nucleoside antibiotics, ascamycin and its dealanyl derivative. In contrast to the broad antibacterial activity of dealanylascamycin against various...
An ordered clone bank for chromosome I of Saccharomyces cerevisiae.
Tanaka, S, Yoshikawa, A, Isono, K
Chromosome I of Saccharomyces cerevisiae DC5 rho 0 was dissected into segments with an average size of 14.0 kb and cloned into lambda phage vectors. The physical maps of the resultant clones,...
Kang, W, Matsushita, Y, Grohmann, L, Graack, H R, Kitakawa, M, Isono, K
The N-terminal amino acid sequence of a large subunit protein, termed YmL33, of the mitochondrial ribosome of the yeast Saccharomyces cerevisiae was determined. The data were obtained to synthesize...
Tanaka, Y, Tsujimura, A, Fujita, N, Isono, S, Isono, K
The chromosomal DNA fragments of Escherichia coli K-12 were cloned into a mini-F cosmid, pRE435, after partial digestion with restriction endonuclease Sau3AI. The clones were first screened for PyrC+...
Satoh, H, Nishida, H, Isono, K
The gene for an alpha-amylase cloned from strain DY-5 of Bacillus stearothermophilus was used to examine to what extent the corresponding genes are structurally similar in other B. stearothermophilus...
Location of a gene (ssrA) for a small, stable RNA (10Sa RNA) in the Escherichia coli chromosome.
Oh, B K, Chauhan, A K, Isono, K, Apirion, D
The gene for 10Sa RNA, which is a major small, stable RNA in Escherichia coli, is a unique gene in the E. coli chromosome. The 10Sa RNA gene (ssrA) has been located between 2,760 and 2,761 kilobases...
Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.
Kitakawa, M, Dabbs, E R, Isono, K
Mutants with alterations in the structural genes for ribosomal proteins S15, L21, and L27 were used in mapping the genes coding for these proteins. Results from P1kc-mediated transductions indicate...
Lipopolysaccharide from Klebsiella pneumoniae inhibits Na+ absorption in canine tracheal epithelium.
Tamaoki, J, Sakai, N, Isono, K, Kanemura, T, Takeyama, K, Takizawa, T
The effect of lipopolysaccharide (LPS) from Klebsiella pneumoniae on the bioelectric properties of canine cultured tracheal epithelium was examined. LPS decreased short-circuit current (Isc), and its...
Gene encoding cytoskeletal proteins in Drosophila rhabdomeres.
Matsumoto, H, Isono, K, Pye, Q, Pak, W L
The ninaC gene is one of eight nina (neither inactivation nor afterpotential) genes identified from mutations that drastically reduce the amount of rhodopsin in the compound eye of Drosophila...
During the course of systematic nucleotide sequence analysis of the terC region of E.coli K-12 by using the ordered lambda phage clones, we found the presence of a gene, termed hrpA, that showed a...
Most of the 155 transcripts of the genes on chromosome III of S.cerevisiae that were identified by a series of Northern hybridization experiments (Yoshikawa, A. and Isono, K., Yeast 6, 383-401, 1990)...
Physical dissection and characterization of chromosomes V and VIII of Saccharomyces cerevisiae.
Chromosomes V and VIII of S. cerevisiae were dissected and ordered clone banks were constructed and characterized. Each bank contains almost the entire chromosome from the left to the right telomere...
Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.
Yura, T, Mori, H, Nagai, H, Nagata, T, Ishihama, A, Fujita, N, ...
A contiguous 111,402-nucleotide sequence corresponding to the 0 to 2.4 min region of the E. coli chromosome was determined as a first step to complete structural analysis of the genome. The resulting...
The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1.
The DNA sequence of the gene rpsA as well as of its neighboring regions has been determined using the dideoxyribonucleotide method. It was found that there is an "open-reading-frame" of 350 bp which...
A computer program package for storing and retrieving DNA/RNA and protein sequence data.
A computer program package has been made available which contains five compound programs written in FORTRAN 10 (for DECsystem-10). Program DATBAS is for storing and improving DNA sequence data,...
Computer programs to analyze DNA and amino acid sequence data.
Extensive modifications have been incorporated into many of the computer programs written by Staden (1-4) to make them easier to cope with DNA and amino acid sequence data. These programs can be...
The SSP1 gene of Schizosaccharomyces pombe which is homologous to the SSC1 gene of Saccharomyces cerevisiae was cloned and its nucleotide sequence determined. A heat-shock element and three possible...
Kitakawa, M, Grohmann, L, Graack, H R, Isono, K
The genes for two large subunit proteins, YmL8 and YmL20, of the mitochondrial ribosome of Saccharomyces cerevisiae were cloned by hybridization with synthetic oligonucleotide mixtures corresponding...
By comparing sequences of restriction enzyme cleavage sites and their distance data, we sorted 384 lambda phage clones containing segments of chromosome VI of S. cerevisiae and constructed an ordered...
Kasai, H, Isono, S, Kitakawa, M, Mineno, J, Akiyama, H, Kurnit, D M, ...
We have developed a strategy for efficient sequence analysis of the genome of E. coli K-12 using insertions of a Tn5-derived mini-transposon into overlapping ordered lambda phage clones to provide...
Lack of ribosomal protein S1 in Bacillus stearothermophilus.
The 30S ribosomal subunit of Bacillus stearothermophilus migrated as a single band when electrophoresed on agarose-acrylamide composite gels. The addition of the ribosomal protein S1 purified from...
Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA.
Schnier, J, Kimura, M, Foulaki, K, Subramanian, A R, Isono, K, Wittmann-Liebold, B
The primary structure of proteins S1, the largest protein component of the Escherichia coli ribosome, has been elucidated by determining the amino acid sequence of the protein (from E. coli MRE600)...
A mutant of Escherichia coli K-12 isolated for temperature-sensitive growth was found to harbor an alteration in robosomal protein L9. Because the chromosomal location of the structural gene for this...
Kanno, M, Hasegawa, M, Ishida, A, Isono, K, Taniguchi, M
The mammalian mel-18/bmi-1 gene products share an amino acid sequence and a secondary structure, including a RING-finger motif, with the Drosophila Polycomb group (PcG) gene products Psc and Su(z)2,...
Takai, A, Sasaki, K, Nagai, H, Mieskes, G, Isobe, M, Isono, K, ...
Several groups have reported that okadaic acid (OA) and some other tight-binding protein phosphatase inhibitors including microcystin-LR (MCLR), calyculin-A and tautomycin prevent each other from...
Purification and characterization of ascamycin-hydrolysing aminopeptidase from Xanthomonas citri.
A nucleoside antibiotic, ascamycin (9-beta-[5'-0-(N-L-alanyl) sulphamoyl-D-ribofuranosyl]-2-chloroadenine), has a selective antibacterial activity against Xanthomonas species. When ascamycin was...
Taste Sensitivity to Trehalose and Its Alteration by Gene Dosage in Drosophila Melanogaster
Tanimura, T., Isono, K., Yamamoto, M. T.
The taste sensitivity to the disaccharide trehalose of Drosophila melanogaster is under the genetic control by the Tre gene on the X chromosome. The gene is genetically dimorphic for high and low...
Tamaoki, J, Tagaya, E, Isono, K, Kondo, M, Konno, K
To elucidate whether K+ channels play a role in the action of epithelium-dependent bronchodilatation, we studied responses in human bronchial strips in the presence of indomethacin and...
Tamaoki, J, Tagaya, E, Nishimura, K, Isono, K, Nagai, A
Sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) plays a role in the regulation of vascular tone, but contribution of this enzyme to nitrovasodilator-induced pulmonary vasodilatation remains...
Stimulation by menthol of Cl secretion via a Ca(2+)-dependent mechanism in canine airway epithelium.
Chiyotani, A., Tamaoki, J., Takeuchi, S., Kondo, M., Isono, K., Konno, K.
1. To investigate the effect of menthol on airway epithelial ion transport function, we studied the bioelectrical properties of canine cultured tracheal epithelium by Ussing's short-circuit technique...
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction
Tamaoki, J., Chiyotani, A., Tagaya, E., Isono, K., Konno, K.
To elucidate the modulatory role of histamine-degrading enzymes in airway constrictor responses, human bronchial strips were studied under isometric conditions in vitro. Pretreatment of tissues with...
Matsushita, K., Takenouchi, T., Kobayashi, S., Hayashi, H., Okuyama, K., Ochiai, T., ...
The authors attempted to investigate the host's immune response against colorectal carcinoma through the expression of HLA-DR antigen on carcinoma cells (Ca) on normal epithelia immediately adjacent...
Terashima, M., Hayashi, K., Fukushima, M., Ide, H., Iizuka, T., Kakegawa, T., ...
A total of 83 specimens of surgically resected tumours from 78 patients with oesophageal cancer were assayed for drug sensitivity using an adhesive tumour cell culture system (LifeTrac CSA assay)....