Lutz, P.M., Isselbacher, K.J., Bernards, R.A.
We have isolated a cDNA encoding a transcription factor that binds to the enhancer of major histocompatibility complex (MHC) class I genes. MHC-binding protein 2 (MBP-2) is a 275-kDa protein,...
Shioda, T, Fenner, M H, Isselbacher, K J
Messenger RNA transcripts of the highly pigmented murine melanoma B16-F1 cells were compared with those from their weakly pigmented derivative B16-F10 cells by differential display. A novel gene...
FitzGerald, M G, Harkin, D P, Silva-Arrieta, S, MacDonald, D J, Lucchina, L C, Unsal, H, ...
Five to ten percent of individuals with melanoma have another affected family member, suggesting familial predisposition. Germ-line mutations in the cyclin-dependent kinase (CDK) inhibitor p16 have...
An increased risk of Crohn's disease in individuals who inherit the HLA class II DRB3*0301 allele.
Forcione, D G, Sands, B, Isselbacher, K J, Rustgi, A, Podolsky, D K, Pillai, S
The role of inflammatory T cells in Crohn's disease suggests that inherited variations in major histocompatibility complex (MHC) class II genes may be of pathogenetic importance in inflammatory bowel...
Truncated WT1 mutants alter the subnuclear localization of the wild-type protein.
Englert, C, Vidal, M, Maheswaran, S, Ge, Y, Ezzell, R M, Isselbacher, K J, ...
WT1 encodes a zinc-finger protein, expressed as distinct isoforms, that is inactivated in a subset of Wilms tumors. Both constitutional and somatic mutations disrupting the DNA-binding domain of WT1...
Acute hepatitis in rats expressing human hepatitis B virus transgenes.
Takahashi, H, Fujimoto, J, Hanada, S, Isselbacher, K J
The molecular mechanisms responsible for hepatocyte death and the events leading to viral clearance in hepatitis B virus (HBV) infections are not well understood. Elucidation of the mechanisms...
Genetic heterogeneity of hepatocellular carcinoma.
Unsal, H, Yakicier, C, Marçais, C, Kew, M, Volkmann, M, Zentgraf, H, ...
We studied 80 hepatocellular carcinomas from three continents for p53 gene (TP53) mutations and hepatitis B virus (HBV) sequences. p53 mutations were frequent in tumors from Mozambique but not in...
Aoki, Y, Isselbacher, K J, Cherayil, B J, Pillai, S
Proteins that bind to discrete domains of the Blk, Fyn, Lyn, and Btk protein tyrosine kinases were examined in pre-B cells that had not been subjected to any external stimulation, as well as in...
Aoki, Y, Isselbacher, K J, Pillai, S
The gene encoding Bruton tyrosine kinase (Btk) is known to be mutated in human X chromosome-linked agammaglobulinemia and in the Xid mouse. This kinase was examined in B lymphocytes before and after...
Shioda, T, Ohta, T, Isselbacher, K J, Rhoads, D B
We examined changes in the mRNA level of SGLT1, a Na+/glucose cotransporter, by the differentiation status of LLC-PK1 renal epithelial cells. Proliferating (undifferentiated) cells revealed no...
A dominant-negative mutant of Max that inhibits sequence-specific DNA binding by Myc proteins.
Billaud, M, Isselbacher, K J, Bernards, R
Myc proteins are basic helix-loop-helix/leucine-zipper proteins that bind to specific DNA sequences. In vivo, Myc proteins have been found associated with Max, another basic...
Rosenwald, I B, Rhoads, D B, Callanan, L D, Isselbacher, K J, Schmidt, E V
Although activation of c-myc is a critical step in the development of lymphomas and other tumors, its normal function(s) in cell growth remain obscure because few myc-regulated genes are known. myc...
Galvin, K, Krishna, S, Ponchel, F, Frohlich, M, Cummings, D E, Carlson, R, ...
A 90-kDa phosphoprotein (p90) of the endoplasmic reticulum was identified by a monoclonal antibody generated against human hepatoma cells. Pulse-chase experiments with [32P]phosphate and...
Van 't Veer, L J, Lutz, P M, Isselbacher, K J, Bernards, R
We have isolated a cDNA encoding a transcription factor that binds to the enhancer of major histocompatibility complex (MHC) class I genes. MHC-binding protein 2 (MBP-2) is a 275-kDa protein,...
Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations.
Børresen, A L, Hovig, E, Smith-Sørensen, B, Malkin, D, Lystad, S, Andersen, T I, ...
At present, mutation of the p53 gene appears to be the most common genetic alteration found in human cancers. These mutations can occur within many different regions of the gene. We have developed a...
Abnormal structure and expression of p53 gene in human hepatocellular carcinoma.
Bressac, B, Galvin, K M, Liang, T J, Isselbacher, K J, Wands, J R, Ozturk, M
There is little information regarding the molecular mechanisms of hepatocarcinogenesis. We studied the p53 gene at the DNA, RNA, and protein level in seven human hepatocellular carcinoma...
Simon, B, Podolsky, D K, Moldenhauer, G, Isselbacher, K J, Gattoni-Celli, S, Brand, S J
The cell surface antigen, epithelial glycoprotein, defined by the monoclonal antibody HEA 125, is expressed on virtually all epithelial cell membranes but not on mesodermal or neural cell membranes....
Shih, C, Burke, K, Chou, M J, Zeldis, J B, Yang, C S, Lee, C S, ...
The open circular genome of human hepatitis B virus (HBV) is known to contain a partially double-stranded DNA with a single-stranded gap region of variable length. This circular structure of the...
Tissue-specific expression of the rat galanin gene.
Kaplan, L M, Spindel, E R, Isselbacher, K J, Chin, W W
We have isolated and characterized cDNAs encoding rat galanin from a cDNA library prepared from rat hypothalamic tissue. Analysis of these clones reveals that rat galanin is synthesized initially as...
Cell-surface changes associated with transformation of human hepatocytes to the malignant phenotype.
Wilson, B, Ozturk, M, Takahashi, H, Motté, P, Kew, M, Isselbacher, K J, ...
Hepatocellular carcinoma is one of the leading causes of cancer death in the world. To understand the cellular changes associated with transformation of hepatocytes to the malignant state, we have...
Sodium butyrate increases glucose transporter expression in LLC-PK1 cells.
Takano, M, Rhoads, D B, Isselbacher, K J
The effect of sodium butyrate on the expression of the facilitated glucose transporter (GT) was investigated in the pig kidney cell line LLC-PK1. When cells were treated with butyrate, GT mRNA...
Doxorubicin conjugates of monoclonal antibodies to hepatoma-associated antigens.
Shouval, D, Adler, R, Wands, J R, Hurwitz, E, Isselbacher, K J, Sela, M
A panel of six murine monoclonal antibodies against hepatocellular carcinoma-associated antigens, reactive with PLC/PRF/5 human hepatoma cells, was conjugated to Adriamycin (doxorubicin) via a...
Gattoni-Celli, S, Willett, C G, Rhoads, D B, Simon, B, Strauss, R M, Kirsch, K, ...
Many human tumors, particularly those of epithelial origin, appear to express greatly reduced levels of major histocompatibility complex class I antigens on their surface. It has been previously...
Evidence for expression of the facilitated glucose transporter in rat hepatocytes.
Rhoads, D B, Takano, M, Gattoni-Celli, S, Chen, C C, Isselbacher, K J
The eukaryotic facilitated glucose transporter (GT) is expressed by many cell types, with the notable exception of hepatocytes; however, GT is expressed by several hepatoma cell lines, including the...
Sunday, M E, Isselbacher, K J, Gattoni-Celli, S, Willett, C G
The major histocompatibility complex (MHC) class I molecules are known to serve as recognition elements for cytotoxic T cells in mediating the rejection of transplanted tumors. We demonstrate that...
Transgenic mouse model for human gastric carcinoma.
Koike, K, Hinrichs, S H, Isselbacher, K J, Jay, G
To understand the pathogenesis that may be induced by human adenovirus type 12 (Ad12), we have generated transgenic mice carrying the Ad12 early region 1 under control of the mouse mammary tumor...
Cell-mediated immunity in acute and chronic hepatitis.
Wands, J R, Perrotto, J L, Alpert, E, Isselbacher, K J
Peripheral lymphocytes from patients with hepatitis-B surface antigen (HBsAg)-positive and -negative acute hepatitis (AH), chronic active hepatitis (CAH), chronic persistent hepatitis (CPH), and...
Wands, J R, Mann, E, Alpert, E, Isselbacher, K J
Circulating immune complexes were identified in cryoproteins isolated from serial samples of serum from six patients with acute viral hepatitis with and without arthritic symptoms. Cryoprecipitates...
Fat Absorption During Inhibition of Protein Synthesis: Studies of Lymph Chylomicrons
Glickman, R. M., Kirsch, K., Isselbacher, K. J.
The effect of protein synthesis inhibition on the absorption of oleic acid from micellar solution was studied in mesenteric lymph fistula rats. A micellar solution of oleic acid labeled with tracer...
Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
Wands, J R, Carlson, R I, Schoemaker, H, Isselbacher, K J, Zurawski, V R
High-affinity monoclonal IgG and IgM antibodies to hepatitis B surface antigen (HBsAg) have been prepared and their functional capabilities explored by means of solid-phase radioimmunoassays....
Kurokawa, T, Tillotson, L G, Chen, C C, Isselbacher, K J
The D-glucose transporter in the human erythrocyte membranes was photoaffinity-labeled with [3H]cytochalasin B and solubilized with n-octyl beta-D-glucopyranoside (octyl glucoside). [3H]Cytochalasin...
Human erythrocyte glucose transporter: normal asymmetric orientation and function in liposomes.
Chen, C C, Kurokawa, T, Shaw, S Y, Tillotson, L G, Kalled, S, Isselbacher, K J
The transport function and orientation of the reconstituted human erythrocyte glucose transporter was studied with liposomes made with bovine brain lipid or Escherichia coli lipid. Reconstitution was...
Rapid identification of low level hepatitis B-related viral genome in serum.
Liang, T J, Isselbacher, K J, Wands, J R
A sensitive and specific method has been developed to detect hepatitis B virus (HBV) in serum. The method involves two steps: the capture of viral genome from serum using a high affinity IgM...
Podolsky, D. K., Weiser, M. M., Isselbacher, K. J.
A galactosyltransferase glycopeptide acceptor purified from human malignant effusions was tested for its effects on cell growth in vitro and in vivo. Addition of the glycopeptide to the media of...
Nishino, H, Christopher, C W, Schiller, R M, Gammon, M T, Ullrey, D, Isselbacher, K J
Enhanced alpha-aminoisobutyric acid transport by hamster cells cultured in the absence of D-glucose has been demonstrated in isolated membrane vesicles. The observed enhancement was seen in the...
Characterization of monoclonal antibodies to serum galactosyltransferase.
Podolsky, D K, Isselbacher, K J
Monoclonal IgG and IgM antibodies (mAbs) directed against serum galactosyltransferase (GalTase) activities were prepared and characterized for their relative specificity for GalTase isoenzymes I and...
Bellet, D H, Wands, J R, Isselbacher, K J, Bohuon, C
A rapid multisite radioimmunoassay for measurement of human alpha-fetoprotein (AFP) that uses two high-affinity monoclonal antibodies directed against distinct and separate determinants on the...
Hepatitis B viral antigenic structure: signature analysis by monoclonal radioimmunoassays.
Wands, J R, Wong, M A, Shorey, J, Brown, R D, Marciniak, R A, Isselbacher, K J
An approach has been developed for the analysis of hepatitis B viral (HBV) antigenic structure that creates numerical "signatures" of HBV strains. This technique employs high-affinity IgM and IgG...
Shouval, D, Wands, J R, Zurawski, V R, Isselbacher, K J, Shafritz, D A
High-affinity 125I-labelled monoclonal antibodies to hepatitis B virus surface antigen (HBsAg) bind to human hepatocellular carcinoma cell line, PLC/PRF/5, which synthesizes and secretes HBsAg. These...
Wands, J R, Bruns, R R, Carlson, R I, Ware, A, Menitove, J E, Isselbacher, K J
Using a monoclonal IgM antibody (anti-HBs) to hepatitis B surface antigen (HBsAg) in a radioimmunoassay for hepatitis B, we have detected high binding activity in human serum that was unreactive in...
Pessin, J E, Tillotson, L G, Yamada, K, Gitomer, W, Carter-Su, C, Mora, R, ...
When deprived of D-glucose for 24 hr, chicken embryo fibroblasts exhibit a marked increase in hexose transport activity compared with that of control cells. Scatchard analysis of [3H]cytochalasin B...
Shafritz, D A, Lieberman, H M, Isselbacher, K J, Wands, J R
High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays...
Hauri, H P, Quaroni, A, Isselbacher, K J
Two monoclonal antibodies, designated BB 3/34/12 and BB 5/8/40/90, have been produced to rat intestinal sucrase/isomaltase (SI) by the hybridoma technique using microvillus membranes as antigen. The...
Regulation of glucose transporters in LLC-PK1 cells: effects of D-glucose and monosaccharides.
Ohta, T, Isselbacher, K J, Rhoads, D B
Regulation of D-glucose transport in the porcine kidney epithelial cell line LLC-PK1 was examined. To identify the sodium-coupled glucose transporter (SGLT), we cloned and sequenced several partial...
Barra, Y, Tanaka, K, Isselbacher, K J, Khoury, G, Jay, G
The identification of a unique major histocompatibility complex class I gene, designated Q10, which encodes a secreted rather than a cell surface antigen has led to questions regarding its potential...
Gattoni-Celli, S, Kirsch, K, Kalled, S, Isselbacher, K J
Type C-related human endogenous retroviral sequences have been previously discovered and characterized [Martin, M. A., Bryan, T., Rasheed, S. & Khan, A.S. (1981) Proc. Natl. Acad. Sci. USA 78,...
Identification and transmission of hepatitis B virus-related variants.
Wands, J R, Fujita, Y K, Isselbacher, K J, Degott, C, Schellekens, H, Dazza, M C, ...
We have identified long-incubation viral agents that share epitopes with hepatitis B virus (HBV). During chimpanzee infectivity studies, these agents may be recognized in the liver since they possess...
Developmental and tissue-specific regulation of the Q10 class I gene by DNA methylation.
Tanaka, K, Barra, Y, Isselbacher, K J, Khoury, G, Jay, G
The H-2 class I genes encode cell-surface glycoproteins that play a critical role in the immune presentation of aberrant cells. The Q10 class I gene, however, encodes a secreted glycoprotein that is...
Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.
Vrba, R, Alpert, E, Isselbacher, K J
Carcinoembryonic antigen (CEA) preparations, from various sources were compared by radioimmunoassay. The preparations studied included four CEA standards (CEA-Roch, CEA-Montreal, CEA-City of Hope,...
Nishino, H, Tillotson, L G, Schiller, R M, Inui, K I, Isselbacher, K J
Plasma membrane fractions isolated from mouse fibroblast BALB/c 3T3 cells transformed by simian virus 40 were partially purified by treatment with dimethylmaleic anhydride followed by extraction with...
Fibronectin synthesis by epithelial crypt cells of rat small intestine.
Quaroni, A, Isselbacher, K J, Ruoslahti, E
Synthesis of fibronectin in an epithelial cell line (IEC-6) established from rat small intestine was demonstrated by using immunofluorescence, radioimmunoassay, and collagen-binding. Internally...
Falchuk, K R, Walker, W A, Perrotto, J L, Isselbacher, K J
The effects of vitamin A on the immune response to bovine serum albumin (BSA) were studied in adult mice. Treatment with vitamin A by the intragastric or parenteral routes markedly increased the...
Carlson, R I, Ben-Porath, E, Shouval, D, Strauss, W, Isselbacher, K J, Wands, J R
Several libraries of monoclonal antibodies have been produced by immunization of Balb/c mice with single cell suspensions of nontrypsin-treated human hepatocellular carcinoma cell (HCC) lines in...
Structural analysis of hepatitis B surface antigen by monoclonal antibodies.
Ben-Porath, E, Wands, J R, Marciniak, R A, Wong, M A, Hornstein, L, Ryder, R, ...
A method has been developed for the analysis of hepatitis B surface antigen (HBsAg) antigenic structure at the molecular level that creates "fingerprints" or "signatures" of various hepatitis B viral...
Increased Uptake of Amino Acids and 2-Deoxy-D-Glucose by Virus-Transformed Cells in Culture
Transformed and nontransformed cells in tissue culture differ in their rate of uptake of certain nutrients, as determined by a polyester-coverslip technique. A 2.5- to 3.5-fold increased rate of...
Quinlan, D C, Parnes, J R, Shalom, R, Garvey, T Q, Isselbacher, K J, Hochstadt, J
Mediated uptake of amino acids by membrane vesicles isolated from Balb/c 3T3 cells transformed by simian virus 40 has been demonstrated. Initial rates of transport of radioactively labeled L-leucine...
Wands, J R, Podolsky, D K, Isselbacher, K J
A monovalent form of concanavalin A (m-Con A) has been prepared to determine the importance of valence for human lymphocyte surface binding and subsequent lymphocyte stimulation as measured by blast...
LaMont, J T, Gammon, M T, Isselbacher, K J
Cell-surface galactosyltransferase was studied in suspensions of intact baby hamster kidney fibroblasts with both endogenous and exogenous glycoprotein acceptors. The cell-surface location of...
Freedman, R A, Weiser, M M, Isselbacher, K J
Intestinal Ca2+ transport was studied in membrane vesicles isolated from microvillus, Golgi, and lateral-basal membrane preparations. Ca2+ uptake by these vesicles was measured by determination of...
Lymphocyte cytotoxicity to autologous liver cells in chronic active hepatitis.
Lymphocyte function in vitro was evaluated in patients with chronic active hepatitis and compared to normal controls. Circulating lymphocytes of patients were spontaneously cytotoxic to 51Cr-labeled...
Composition of human colonic mucin. Selective alteration in inflammatory bowel disease.
Podolsky, D K, Isselbacher, K J
Human colonic mucin has been isolated from mucosal scrapings of fresh surgical specimens of normal controls as well as patients with Crohn's colitis and ulcerative colitis. Following sonication and...
Rat intestinal trehalase. Studies of the active site.
Chen, C C, Guo, W J, Isselbacher, K J
Rat intestinal trehalase was solubilized, purified and reconstituted into proteoliposomes. With octyl glucoside as the solubilizing detergent, the purified protein appeared as a single band on...
Surface-membrane biogenesis in rat intestinal epithelial cells at different stages of maturation.
Quaroni, A, Kirsch, K, Herscovics, A, Isselbacher, K J
The biosynthesis of membrane proteins and glycoproteins has been studied in rat intestinal crypt and villus cells by measuring the incorporation of L-[5,6-3H] fucose, D-[2-3H] mannose and...
Douglas, A. P., Kerley, R., Isselbacher, K. J.
A technique is described for the isolation of a plasma-membrane fraction from the rat intestinal epithelial cell which is distinct from the microvillus membrane of that cell. The isolated fraction...
Lipid composition of the isolated rat intestinal microvillus membrane
Forstner, G. G., Tanaka, K., Isselbacher, K. J.
1. Rat intestinal microvillus plasma membranes were prepared from previously isolated brush borders and the lipid composition was analysed. 2. The molar ratio of cholesterol to phospholipid was...
Rat intestinal microvillus membranes. Purification and biochemical characterization
Forstner, G. G., Sabesin, S. M., Isselbacher, K. J.
1. A technique is described for the removal of subcellular contaminants from intact rat intestinal brush borders, and for the subsequent separation of a microvillus membrane fraction from a fibrillar...
Shioda, T, Fenner, M H, Isselbacher, K J
Messenger RNA transcripts of the highly pigmented murine melanoma B16-F1 cells were compared with those from their weakly pigmented derivative B16-F10 cells by differential display. A novel gene...
FitzGerald, M G, Harkin, D P, Silva-Arrieta, S, MacDonald, D J, Lucchina, L C, Unsal, H, ...
Five to ten percent of individuals with melanoma have another affected family member, suggesting familial predisposition. Germ-line mutations in the cyclin-dependent kinase (CDK) inhibitor p16 have...
An increased risk of Crohn's disease in individuals who inherit the HLA class II DRB3*0301 allele.
Forcione, D G, Sands, B, Isselbacher, K J, Rustgi, A, Podolsky, D K, Pillai, S
The role of inflammatory T cells in Crohn's disease suggests that inherited variations in major histocompatibility complex (MHC) class II genes may be of pathogenetic importance in inflammatory bowel...
Truncated WT1 mutants alter the subnuclear localization of the wild-type protein.
Englert, C, Vidal, M, Maheswaran, S, Ge, Y, Ezzell, R M, Isselbacher, K J, ...
WT1 encodes a zinc-finger protein, expressed as distinct isoforms, that is inactivated in a subset of Wilms tumors. Both constitutional and somatic mutations disrupting the DNA-binding domain of WT1...
Acute hepatitis in rats expressing human hepatitis B virus transgenes.
Takahashi, H, Fujimoto, J, Hanada, S, Isselbacher, K J
The molecular mechanisms responsible for hepatocyte death and the events leading to viral clearance in hepatitis B virus (HBV) infections are not well understood. Elucidation of the mechanisms...
Genetic heterogeneity of hepatocellular carcinoma.
Unsal, H, Yakicier, C, Marçais, C, Kew, M, Volkmann, M, Zentgraf, H, ...
We studied 80 hepatocellular carcinomas from three continents for p53 gene (TP53) mutations and hepatitis B virus (HBV) sequences. p53 mutations were frequent in tumors from Mozambique but not in...
Aoki, Y, Isselbacher, K J, Cherayil, B J, Pillai, S
Proteins that bind to discrete domains of the Blk, Fyn, Lyn, and Btk protein tyrosine kinases were examined in pre-B cells that had not been subjected to any external stimulation, as well as in...
Aoki, Y, Isselbacher, K J, Pillai, S
The gene encoding Bruton tyrosine kinase (Btk) is known to be mutated in human X chromosome-linked agammaglobulinemia and in the Xid mouse. This kinase was examined in B lymphocytes before and after...
Shioda, T, Ohta, T, Isselbacher, K J, Rhoads, D B
We examined changes in the mRNA level of SGLT1, a Na+/glucose cotransporter, by the differentiation status of LLC-PK1 renal epithelial cells. Proliferating (undifferentiated) cells revealed no...
A dominant-negative mutant of Max that inhibits sequence-specific DNA binding by Myc proteins.
Billaud, M, Isselbacher, K J, Bernards, R
Myc proteins are basic helix-loop-helix/leucine-zipper proteins that bind to specific DNA sequences. In vivo, Myc proteins have been found associated with Max, another basic...
Rosenwald, I B, Rhoads, D B, Callanan, L D, Isselbacher, K J, Schmidt, E V
Although activation of c-myc is a critical step in the development of lymphomas and other tumors, its normal function(s) in cell growth remain obscure because few myc-regulated genes are known. myc...
Galvin, K, Krishna, S, Ponchel, F, Frohlich, M, Cummings, D E, Carlson, R, ...
A 90-kDa phosphoprotein (p90) of the endoplasmic reticulum was identified by a monoclonal antibody generated against human hepatoma cells. Pulse-chase experiments with [32P]phosphate and...
Van 't Veer, L J, Lutz, P M, Isselbacher, K J, Bernards, R
We have isolated a cDNA encoding a transcription factor that binds to the enhancer of major histocompatibility complex (MHC) class I genes. MHC-binding protein 2 (MBP-2) is a 275-kDa protein,...
Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations.
Børresen, A L, Hovig, E, Smith-Sørensen, B, Malkin, D, Lystad, S, Andersen, T I, ...
At present, mutation of the p53 gene appears to be the most common genetic alteration found in human cancers. These mutations can occur within many different regions of the gene. We have developed a...
Abnormal structure and expression of p53 gene in human hepatocellular carcinoma.
Bressac, B, Galvin, K M, Liang, T J, Isselbacher, K J, Wands, J R, Ozturk, M
There is little information regarding the molecular mechanisms of hepatocarcinogenesis. We studied the p53 gene at the DNA, RNA, and protein level in seven human hepatocellular carcinoma...
Simon, B, Podolsky, D K, Moldenhauer, G, Isselbacher, K J, Gattoni-Celli, S, Brand, S J
The cell surface antigen, epithelial glycoprotein, defined by the monoclonal antibody HEA 125, is expressed on virtually all epithelial cell membranes but not on mesodermal or neural cell membranes....
Shih, C, Burke, K, Chou, M J, Zeldis, J B, Yang, C S, Lee, C S, ...
The open circular genome of human hepatitis B virus (HBV) is known to contain a partially double-stranded DNA with a single-stranded gap region of variable length. This circular structure of the...
Tissue-specific expression of the rat galanin gene.
Kaplan, L M, Spindel, E R, Isselbacher, K J, Chin, W W
We have isolated and characterized cDNAs encoding rat galanin from a cDNA library prepared from rat hypothalamic tissue. Analysis of these clones reveals that rat galanin is synthesized initially as...
Cell-surface changes associated with transformation of human hepatocytes to the malignant phenotype.
Wilson, B, Ozturk, M, Takahashi, H, Motté, P, Kew, M, Isselbacher, K J, ...
Hepatocellular carcinoma is one of the leading causes of cancer death in the world. To understand the cellular changes associated with transformation of hepatocytes to the malignant state, we have...
Sodium butyrate increases glucose transporter expression in LLC-PK1 cells.
Takano, M, Rhoads, D B, Isselbacher, K J
The effect of sodium butyrate on the expression of the facilitated glucose transporter (GT) was investigated in the pig kidney cell line LLC-PK1. When cells were treated with butyrate, GT mRNA...
Doxorubicin conjugates of monoclonal antibodies to hepatoma-associated antigens.
Shouval, D, Adler, R, Wands, J R, Hurwitz, E, Isselbacher, K J, Sela, M
A panel of six murine monoclonal antibodies against hepatocellular carcinoma-associated antigens, reactive with PLC/PRF/5 human hepatoma cells, was conjugated to Adriamycin (doxorubicin) via a...
Gattoni-Celli, S, Willett, C G, Rhoads, D B, Simon, B, Strauss, R M, Kirsch, K, ...
Many human tumors, particularly those of epithelial origin, appear to express greatly reduced levels of major histocompatibility complex class I antigens on their surface. It has been previously...
Evidence for expression of the facilitated glucose transporter in rat hepatocytes.
Rhoads, D B, Takano, M, Gattoni-Celli, S, Chen, C C, Isselbacher, K J
The eukaryotic facilitated glucose transporter (GT) is expressed by many cell types, with the notable exception of hepatocytes; however, GT is expressed by several hepatoma cell lines, including the...
Sunday, M E, Isselbacher, K J, Gattoni-Celli, S, Willett, C G
The major histocompatibility complex (MHC) class I molecules are known to serve as recognition elements for cytotoxic T cells in mediating the rejection of transplanted tumors. We demonstrate that...
Transgenic mouse model for human gastric carcinoma.
Koike, K, Hinrichs, S H, Isselbacher, K J, Jay, G
To understand the pathogenesis that may be induced by human adenovirus type 12 (Ad12), we have generated transgenic mice carrying the Ad12 early region 1 under control of the mouse mammary tumor...
Cell-mediated immunity in acute and chronic hepatitis.
Wands, J R, Perrotto, J L, Alpert, E, Isselbacher, K J
Peripheral lymphocytes from patients with hepatitis-B surface antigen (HBsAg)-positive and -negative acute hepatitis (AH), chronic active hepatitis (CAH), chronic persistent hepatitis (CPH), and...
Wands, J R, Mann, E, Alpert, E, Isselbacher, K J
Circulating immune complexes were identified in cryoproteins isolated from serial samples of serum from six patients with acute viral hepatitis with and without arthritic symptoms. Cryoprecipitates...
Fat Absorption During Inhibition of Protein Synthesis: Studies of Lymph Chylomicrons
Glickman, R. M., Kirsch, K., Isselbacher, K. J.
The effect of protein synthesis inhibition on the absorption of oleic acid from micellar solution was studied in mesenteric lymph fistula rats. A micellar solution of oleic acid labeled with tracer...
Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
Wands, J R, Carlson, R I, Schoemaker, H, Isselbacher, K J, Zurawski, V R
High-affinity monoclonal IgG and IgM antibodies to hepatitis B surface antigen (HBsAg) have been prepared and their functional capabilities explored by means of solid-phase radioimmunoassays....
Kurokawa, T, Tillotson, L G, Chen, C C, Isselbacher, K J
The D-glucose transporter in the human erythrocyte membranes was photoaffinity-labeled with [3H]cytochalasin B and solubilized with n-octyl beta-D-glucopyranoside (octyl glucoside). [3H]Cytochalasin...
Human erythrocyte glucose transporter: normal asymmetric orientation and function in liposomes.
Chen, C C, Kurokawa, T, Shaw, S Y, Tillotson, L G, Kalled, S, Isselbacher, K J
The transport function and orientation of the reconstituted human erythrocyte glucose transporter was studied with liposomes made with bovine brain lipid or Escherichia coli lipid. Reconstitution was...
Rapid identification of low level hepatitis B-related viral genome in serum.
Liang, T J, Isselbacher, K J, Wands, J R
A sensitive and specific method has been developed to detect hepatitis B virus (HBV) in serum. The method involves two steps: the capture of viral genome from serum using a high affinity IgM...
Podolsky, D. K., Weiser, M. M., Isselbacher, K. J.
A galactosyltransferase glycopeptide acceptor purified from human malignant effusions was tested for its effects on cell growth in vitro and in vivo. Addition of the glycopeptide to the media of...
Nishino, H, Christopher, C W, Schiller, R M, Gammon, M T, Ullrey, D, Isselbacher, K J
Enhanced alpha-aminoisobutyric acid transport by hamster cells cultured in the absence of D-glucose has been demonstrated in isolated membrane vesicles. The observed enhancement was seen in the...
Characterization of monoclonal antibodies to serum galactosyltransferase.
Podolsky, D K, Isselbacher, K J
Monoclonal IgG and IgM antibodies (mAbs) directed against serum galactosyltransferase (GalTase) activities were prepared and characterized for their relative specificity for GalTase isoenzymes I and...
Bellet, D H, Wands, J R, Isselbacher, K J, Bohuon, C
A rapid multisite radioimmunoassay for measurement of human alpha-fetoprotein (AFP) that uses two high-affinity monoclonal antibodies directed against distinct and separate determinants on the...
Hepatitis B viral antigenic structure: signature analysis by monoclonal radioimmunoassays.
Wands, J R, Wong, M A, Shorey, J, Brown, R D, Marciniak, R A, Isselbacher, K J
An approach has been developed for the analysis of hepatitis B viral (HBV) antigenic structure that creates numerical "signatures" of HBV strains. This technique employs high-affinity IgM and IgG...
Shouval, D, Wands, J R, Zurawski, V R, Isselbacher, K J, Shafritz, D A
High-affinity 125I-labelled monoclonal antibodies to hepatitis B virus surface antigen (HBsAg) bind to human hepatocellular carcinoma cell line, PLC/PRF/5, which synthesizes and secretes HBsAg. These...
Wands, J R, Bruns, R R, Carlson, R I, Ware, A, Menitove, J E, Isselbacher, K J
Using a monoclonal IgM antibody (anti-HBs) to hepatitis B surface antigen (HBsAg) in a radioimmunoassay for hepatitis B, we have detected high binding activity in human serum that was unreactive in...
Pessin, J E, Tillotson, L G, Yamada, K, Gitomer, W, Carter-Su, C, Mora, R, ...
When deprived of D-glucose for 24 hr, chicken embryo fibroblasts exhibit a marked increase in hexose transport activity compared with that of control cells. Scatchard analysis of [3H]cytochalasin B...
Shafritz, D A, Lieberman, H M, Isselbacher, K J, Wands, J R
High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays...
Hauri, H P, Quaroni, A, Isselbacher, K J
Two monoclonal antibodies, designated BB 3/34/12 and BB 5/8/40/90, have been produced to rat intestinal sucrase/isomaltase (SI) by the hybridoma technique using microvillus membranes as antigen. The...
Regulation of glucose transporters in LLC-PK1 cells: effects of D-glucose and monosaccharides.
Ohta, T, Isselbacher, K J, Rhoads, D B
Regulation of D-glucose transport in the porcine kidney epithelial cell line LLC-PK1 was examined. To identify the sodium-coupled glucose transporter (SGLT), we cloned and sequenced several partial...
Barra, Y, Tanaka, K, Isselbacher, K J, Khoury, G, Jay, G
The identification of a unique major histocompatibility complex class I gene, designated Q10, which encodes a secreted rather than a cell surface antigen has led to questions regarding its potential...
Gattoni-Celli, S, Kirsch, K, Kalled, S, Isselbacher, K J
Type C-related human endogenous retroviral sequences have been previously discovered and characterized [Martin, M. A., Bryan, T., Rasheed, S. & Khan, A.S. (1981) Proc. Natl. Acad. Sci. USA 78,...
Identification and transmission of hepatitis B virus-related variants.
Wands, J R, Fujita, Y K, Isselbacher, K J, Degott, C, Schellekens, H, Dazza, M C, ...
We have identified long-incubation viral agents that share epitopes with hepatitis B virus (HBV). During chimpanzee infectivity studies, these agents may be recognized in the liver since they possess...
Developmental and tissue-specific regulation of the Q10 class I gene by DNA methylation.
Tanaka, K, Barra, Y, Isselbacher, K J, Khoury, G, Jay, G
The H-2 class I genes encode cell-surface glycoproteins that play a critical role in the immune presentation of aberrant cells. The Q10 class I gene, however, encodes a secreted glycoprotein that is...
Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.
Vrba, R, Alpert, E, Isselbacher, K J
Carcinoembryonic antigen (CEA) preparations, from various sources were compared by radioimmunoassay. The preparations studied included four CEA standards (CEA-Roch, CEA-Montreal, CEA-City of Hope,...
Nishino, H, Tillotson, L G, Schiller, R M, Inui, K I, Isselbacher, K J
Plasma membrane fractions isolated from mouse fibroblast BALB/c 3T3 cells transformed by simian virus 40 were partially purified by treatment with dimethylmaleic anhydride followed by extraction with...
Fibronectin synthesis by epithelial crypt cells of rat small intestine.
Quaroni, A, Isselbacher, K J, Ruoslahti, E
Synthesis of fibronectin in an epithelial cell line (IEC-6) established from rat small intestine was demonstrated by using immunofluorescence, radioimmunoassay, and collagen-binding. Internally...
Falchuk, K R, Walker, W A, Perrotto, J L, Isselbacher, K J
The effects of vitamin A on the immune response to bovine serum albumin (BSA) were studied in adult mice. Treatment with vitamin A by the intragastric or parenteral routes markedly increased the...
Carlson, R I, Ben-Porath, E, Shouval, D, Strauss, W, Isselbacher, K J, Wands, J R
Several libraries of monoclonal antibodies have been produced by immunization of Balb/c mice with single cell suspensions of nontrypsin-treated human hepatocellular carcinoma cell (HCC) lines in...
Structural analysis of hepatitis B surface antigen by monoclonal antibodies.
Ben-Porath, E, Wands, J R, Marciniak, R A, Wong, M A, Hornstein, L, Ryder, R, ...
A method has been developed for the analysis of hepatitis B surface antigen (HBsAg) antigenic structure at the molecular level that creates "fingerprints" or "signatures" of various hepatitis B viral...
Increased Uptake of Amino Acids and 2-Deoxy-D-Glucose by Virus-Transformed Cells in Culture
Transformed and nontransformed cells in tissue culture differ in their rate of uptake of certain nutrients, as determined by a polyester-coverslip technique. A 2.5- to 3.5-fold increased rate of...
Quinlan, D C, Parnes, J R, Shalom, R, Garvey, T Q, Isselbacher, K J, Hochstadt, J
Mediated uptake of amino acids by membrane vesicles isolated from Balb/c 3T3 cells transformed by simian virus 40 has been demonstrated. Initial rates of transport of radioactively labeled L-leucine...
Wands, J R, Podolsky, D K, Isselbacher, K J
A monovalent form of concanavalin A (m-Con A) has been prepared to determine the importance of valence for human lymphocyte surface binding and subsequent lymphocyte stimulation as measured by blast...
LaMont, J T, Gammon, M T, Isselbacher, K J
Cell-surface galactosyltransferase was studied in suspensions of intact baby hamster kidney fibroblasts with both endogenous and exogenous glycoprotein acceptors. The cell-surface location of...
Freedman, R A, Weiser, M M, Isselbacher, K J
Intestinal Ca2+ transport was studied in membrane vesicles isolated from microvillus, Golgi, and lateral-basal membrane preparations. Ca2+ uptake by these vesicles was measured by determination of...
Lymphocyte cytotoxicity to autologous liver cells in chronic active hepatitis.
Lymphocyte function in vitro was evaluated in patients with chronic active hepatitis and compared to normal controls. Circulating lymphocytes of patients were spontaneously cytotoxic to 51Cr-labeled...
Composition of human colonic mucin. Selective alteration in inflammatory bowel disease.
Podolsky, D K, Isselbacher, K J
Human colonic mucin has been isolated from mucosal scrapings of fresh surgical specimens of normal controls as well as patients with Crohn's colitis and ulcerative colitis. Following sonication and...
Rat intestinal trehalase. Studies of the active site.
Chen, C C, Guo, W J, Isselbacher, K J
Rat intestinal trehalase was solubilized, purified and reconstituted into proteoliposomes. With octyl glucoside as the solubilizing detergent, the purified protein appeared as a single band on...
Surface-membrane biogenesis in rat intestinal epithelial cells at different stages of maturation.
Quaroni, A, Kirsch, K, Herscovics, A, Isselbacher, K J
The biosynthesis of membrane proteins and glycoproteins has been studied in rat intestinal crypt and villus cells by measuring the incorporation of L-[5,6-3H] fucose, D-[2-3H] mannose and...
Douglas, A. P., Kerley, R., Isselbacher, K. J.
A technique is described for the isolation of a plasma-membrane fraction from the rat intestinal epithelial cell which is distinct from the microvillus membrane of that cell. The isolated fraction...
Lipid composition of the isolated rat intestinal microvillus membrane
Forstner, G. G., Tanaka, K., Isselbacher, K. J.
1. Rat intestinal microvillus plasma membranes were prepared from previously isolated brush borders and the lipid composition was analysed. 2. The molar ratio of cholesterol to phospholipid was...
Rat intestinal microvillus membranes. Purification and biochemical characterization
Forstner, G. G., Sabesin, S. M., Isselbacher, K. J.
1. A technique is described for the removal of subcellular contaminants from intact rat intestinal brush borders, and for the subsequent separation of a microvillus membrane fraction from a fibrillar...
Shioda, T., Munn, L. L., Fenner, M. H., Jain, R. K., Isselbacher, K. J.
The early events of metastasis involve multiple interactions between cancer cells and the host microcirculation during cancer cell arrest, adhesion, and extravasation. These interactions may lead to...
Decreased suppressor cell activity in inflammatory bowel disease.
Hodgson, H J, Wands, J R, Isselbacher, K J
Studies were performed on eleven patients with inflammatory bowel disease to determine if there was an alteration in concanavalin A (Con A) induced suppressor cell activity. Similar investigations...
SYSTEMIC CELLULAR HYPERSENSITIVITY INDUCED BY AN INTESTINALLY ABSORBED ANTIGEN
Perrotto, Joseph L., Hang, Le Ming, Isselbacher, K. J., Warren, K. S.
Neonatal mice given intact living Schistosoma mansoni eggs or soluble schistosome egg antigens intragastrically developed systemic cellular hypersensitivity as shown by markedly accelerated,...
Walker, W. A., Cornell, R., Davenport, L. M., Isselbacher, K. J.
The immature small intestine of neonatal mammals is permeable to gamma globulins as a source of passive immunity. Allegedly, macromolecular absorption ceases when the epithelial cell membrane...