Inhibition of airway Na+ transport by respiratory syncytial virus (2007)
Kunzelmann, K, Sun, J, Meanger, J, King, NJ, Cook, DI
In previous studies, we have shown that two major respiratory pathogens, influenza virus and parainfluenza virus, produce acute alterations in ion transport upon contacting the apical membrane of the...
Dawson, P. A., Pirlo, K. J., Steane, S. E., Kunzelmann, K., Chien, Y. J., Markovich, D.
Sulfate is an essential ion required for numerous functions in mammalian physiology. Due to its hydrophilic nature, cells require sulfate transporters on their plasma membranes to allow entry of...
Dawson, P. A., Pirlo, K. J., Steane, S. E., Nguyen, K. A., Kunzelmann, K., Chien, Y. J., ...
Inorganic sulfate is essential for numerous functions in mammalian physiology. In the present study, we characterized the functional properties of the rat Na+-sulfate cotransporter NaS2 (rNaS2),...
Control of ion transport in mammalian airways by protease activated receptors type 2 (PAR-2) (2005)
Kunzelmann, K., Sun, J., Markovich, D., Konig, J., Murle, B., Mall, M., ...
Protease-activated receptors (PARs) are widely distributed in human airways. They couple to G-proteins and are activated after proteolytic cleavage of the N terminus of the receptor. Evidence is...
Markovich, D., Regeer, R. R., Kunzelmann, K., Dawson, P. A.
Sulfate plays an essential role in human growth and development. Here, we characterized the functional properties of the human Na+-sulfate cotransporter (hNaS2), determined its tissue distribution,...
Purinergic inhibition of the epithelial Na+ transport via hydrolysis of PIP2 (2005)
Kunzelmann, K., Bachhuber, T., Regeer, R. R., Markovich, D., Sun, J., Schreiber, R.
Purinergic inhibition of the epithelial Na+ transport via hydrolysis of PIP2 (2004)
Kunzelmann, K, Bachhuber, T, Regeer, R, Markovich, D, Sun, J, Schreiber, R
Stimulation of purinergic receptors inhibits amiloride-sensitive Na+ transport in epithelial tissues by an unknown mechanism. Because previous studies excluded the role of intracellular Ca2+ or...
Acute effects of parainfluenza virus on epithelial electrolyte transport (2004)
Kunzelmann, K., Konig, J., Sun, J., Markovich, D., King, N. J., Karupiah, G., ...
Parainfluenza viruses are important causes of respiratory disease in both children and adults. In particular, they are the major cause of the serious childhood illness croup...
Schreiber, R., Konig, J., Sun, J., Markovich, D., Kunzelmann, K.
Both stimulation of purinergic receptors by ATP and activation of the cystic fibrosis transmembrane conductance regulator (CFTR) inhibit amiloride-sensitive Na+ transport and activate Cl-secretion....
GFP-tagged CFTR transgene is functional in the G551D cystic fibrosis mouse colon (2003)
Oceandy, D., McMorran, B., Schreiber, R., Wainwright, B. J., Kunzelmann, K.
Trafficking of the cystic fibrosis transmembrane conductance regulator (CFTR) is central to its function, with the most common mutation, DeltaF508, resulting in abnormal processing and trafficking....
Schreiber, R, Murle, B, Sun, J, Kunzelmann, K
Recent studies on frog skin acini have challenged the question whether Cl- secretion or Na+ absorption in the airways is driven by luminal K+ channels in series to a basolateral K+ conductance. We...
Ion transport induced by proteinase-activated receptors (PAR2) in colon and airways (2002)
Kunzelmann, K, Schreiber, R, Konig, J, Mall, M
Protease-activated receptors type 2 (PAR2) are activated by serine proteases like trypsin and mast cell tryptase. The function and physiological significance of PAR2 receptors is poorly understood,...
Kunzelmann, K, Schreiber, R, Cook, D
Purinergic stimulation of airway epithelial cells induces Cl- secretion and modulates Na+ absorption by an unknown mechanism. To gain insight into this mechanism, we used a perfused micro-Ussing...
Activation of ion secretion via proteinase-activated receptor-2 in human colon (2002)
Mall, M, Gonska, T, Thomas, J, Hirtz, S, Schreiber, R, Kunzelmann, K
Proteinase-activated receptor (PAR) type 2 (PAR-2) has been shown to mediate ion secretion in cultured epithelial cells and rat jejunum. With the use of a microUssing chamber, we demonstrate the role...
No evidence for inhibition of ENaC through CFTR-mediated release of ATP (2002)
Konig, J, Schreiber, R, Mall, M, Kunzelmann, K
Both purinergic stimulation and activation of cystic fibrosis transmembrane conductance regulator (CFTR) increases Cl- secretion and inhibit amiloride-sensitive Na+ transport. CFTR has been suggested...
Kerst, G, Beschorner, U, Unsold, B, Von Hahn, T, Schreiber, R, Greger, R, ...
KCNQ1 (K(V)LQT1) K+ channels play an important role during electrolyte secretion in airways and colon. KCNQ1 was cloned recently from NaCl-secreting shark rectal glands. Here we study. the properties...
Cystic fibrosis and CFTR (2001)
Greger, R, Schreiber, R, Mall, M, Wissner, A, Hopf, A, Briel, M, ...
Cystic fibrosis (CF) is a complex disease affecting epithelial ion transport. There are not many diseases like CF that have triggered such intense research activities. The complexity of the disease...
Boucherot, A, Schreiber, R, Kunzelmann, K
The K+ channel KCNQ1 (K(V)LQT1) is a voltage-gated K+ channel, coexpressed with regulatory subunits such as KCNE1 (IsK, mink) or KCNE3, depending on the tissue examined. Here, we investigate...
Cloning and function of the rat colonic epithelial K+ channel K(V)LQT1 (2001)
Kunzelmann, K, Hubner, M, Schreiber, R, Levy-Holzman, R, Garty, H, Bleich, M, ...
K(V)LQT1 (K(V)LQ1) is a voltage-gated K+ channel essential for repolarization of the heart action potential that is defective in cardiac arrhythmia. The channel is inhibited by the chromanol 293B, a...
Schreiber, R, Kindle, P, Benzing, T, Walz, G, Kunzelmann, K
The cystic fibrosis transmembrane conductance regulator (CFTR) has been shown previously to be regulated by inhibitory G proteins. In the present study, we demonstrate inhibition of CFTR by...
Control of cystic fibrosis transmembrane conductance regulator expression by BAP31 (2001)
Lambert, G, Becker, B, Schreiber, R, Boucherot, A, Reth, M, Kunzelmann, K
Expression of the cystic fibrosis transmembrane conductance regulator (CFTR) is stringently controlled by molecular chaperones participating in formation of the quality control system. it has been...
Mechanisms of the inhibition of epithelial Na+ channels by CFTR and purinergic stimulation (2001)
Kunzelmann, K, Schreiber, R, Boucherot, A
The epithelial Na+ channel ENaC is inhibited when the cystic fibrosis transmembrane conductance regulator (CFTR) coexpressed in the same cell is activated by the cyclic adenosine monophosphate...
Pharmacotherapy of the ion transport defect in cystic fibrosis (2001)
1. More than 1300 different mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) cause cystic fibrosis (CF), a disease characterized by deficient epithelial Cl- secretion and...
Expression and function of colonic epithelial K(v)LQT1 K+ channels (2001)
Kunzelmann, K, Bleich, M, Warth, R, Levy-Holzman, R, Garty, H, Schreiber, R
1. K(V)LQT1 (KCNQ1) is a voltage-gated K+ channel essential for repolarization of the heart action potential Defects in ion channels have been demonstrated in cardiac arrhythmia. This channel is...
Konig, J, Schreiber, R, Voelcker, T, Mall, M, Kunzelmann, K
Activation of the CFTR Cl- channel inhibits epithelial Na+ channels (ENaC), according to studies on epithelial cells and overexpressing recombinant cells. Here we demonstrate that ENaC is inhibited...
Mall, M, Wissner, A, Seydewitz, HH, Hubner, M, Kuehr, J, Brandis, M, ...
1 The flavonoid genistein has been shown to activate a Cl- conductance in various cell types expressing CFTR. We examined if similar effects can be observed when genistein is applied to native ex...
Mall, M, Wissner, A, Gonska, T, Calenborn, D, Kuehr, J, Brandis, M, ...
Cystic fibrosis (CF) airway epithelia are characterized by enhanced Na+ absorption probably due to a lack of downregulation of epithelial Na+ channels by mutant CF transmembrane conductance...
Influenza virus inhibits amiloride-sensitive Na+ channels in respiratory epithelia
Kunzelmann, K., Beesley, A. H., King, N. J., Karupiah, G., Young, J. A., Cook, D. I.
Many pathogens causing diarrhea do so by modulating ion transport in the gut. Respiratory pathogens are similarly associated with disturbances of fluid balance in the respiratory tract, although it...
Mall, M, Bleich, M, Greger, R, Schreiber, R, Kunzelmann, K
Cystic fibrosis (CF) airway cells, besides their well-known defect in cAMP-dependent Cl- conductance, are characterized by an enhanced Na+ conductance. In this study we have examined the Na+...
Missiaen, L, Parys, J B, De Smedt, H, Sienaert, I, Sipma, H, Vanlingen, S, ...
Ca2+ release from intracellular stores occurs via two families of intracellular channels, each with their own specific agonist: Ins(1, 4,5)P3 for the Ins(1,4,5)P3 receptor and cyclic ADP-ribose...
Busch, A E, Kopp, H G, Waldegger, S, Samarzija, I, Süssbrich, H, Raber, G, ...
1. The effects of isosorbiddinitrate (ISDN) were tested on membrane currents and resting potential in Xenopus laevis oocytes which were either uninjected or injected with cRNA encoding for K+...
Influenza virus inhibits amiloride-sensitive Na+ channels in respiratory epithelia
Kunzelmann, K., Beesley, A. H., King, N. J., Karupiah, G., Young, J. A., Cook, D. I.
Many pathogens causing diarrhea do so by modulating ion transport in the gut. Respiratory pathogens are similarly associated with disturbances of fluid balance in the respiratory tract, although it...
Mall, M, Bleich, M, Greger, R, Schreiber, R, Kunzelmann, K
Cystic fibrosis (CF) airway cells, besides their well-known defect in cAMP-dependent Cl- conductance, are characterized by an enhanced Na+ conductance. In this study we have examined the Na+...
Busch, A E, Kopp, H G, Waldegger, S, Samarzija, I, Süssbrich, H, Raber, G, ...
1. The effects of isosorbiddinitrate (ISDN) were tested on membrane currents and resting potential in Xenopus laevis oocytes which were either uninjected or injected with cRNA encoding for K+...
Missiaen, L, Parys, J B, De Smedt, H, Sienaert, I, Sipma, H, Vanlingen, S, ...
Ca2+ release from intracellular stores occurs via two families of intracellular channels, each with their own specific agonist: Ins(1, 4,5)P3 for the Ins(1,4,5)P3 receptor and cyclic ADP-ribose...
Markovich, D, Regeer, RR, Kunzelmann, K, Dawson, PA
Sulfate plays an essential role in human growth and development. Here, we characterized the functional properties of the human Na+-sulfate cotransporter (hNaS2), determined its tissue distribution,...
Dawson, PA, Pirlo, KJ, Steane, SE, Nguyen, KA, Kunzelmann, K, Chien, YJ, ...
Inorganic sulfate is essential for numerous functions in mammalian physiology. In the present study, we characterized the functional properties of the rat Na+-sulfate cotransporter NaS2 (rNaS2),...
The role of the IsK protein in the specific pharmacological properties of the IKs channel complex
Busch, A E, Busch, G L, Ford, E, Suessbrich, H, Greger, R, ...
IKs channels are composed of IsK and KvLQT1 subunits and underly the slowly activating, voltage-dependent IKs conductance in heart. Although it appears clear that the IsK protein affects both the...
Briel, M, Greger, R, Kunzelmann, K
Epithelial Na+ channels (ENaCs) are inhibited by the cystic fibrosis transmembrane conductance regulator (CFTR) when CFTR is activated by protein kinase A. Since cAMP-dependent activation of CFTR...