Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli.
Quinolone-resistant clinical Escherichia coli isolates were examined for mutations in the marRAB operon of the multiple antibiotic resistance (mar) locus. Among 23 strains evaluated, 8 were chosen...
White, D G, Maneewannakul, K, Von Hofe, E, Zillman, M, Eisenberg, W, Field, A K, ...
The multiple antibiotic resistance operon (marORAB) in Escherichia coli controls intrinsic susceptibility and resistance to multiple, structurally different antibiotics and other noxious agents. A...
Characterization of traX, the F plasmid locus required for acetylation of F-pilin subunits.
Maneewannakul, K, Maneewannakul, S, Ippen-Ihler, K
Acetylation of F-pilin subunits has previously been shown to depend upon expression of the F plasmid transfer operon gene traX. To assess the requirement for pilin acetylation in conjugative transfer...
The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.
Moore, D, Hamilton, C M, Maneewannakul, K, Mintz, Y, Frost, L S, Ippen-Ihler, K
The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies...
Maneewannakul, K, Maneewannakul, S, Ippen-Ihler, K
Transfer of the Escherichia coli fertility plasmid, F, is dependent on expression of F pili. Synthesis of F-pilin subunits is known to involve three F plasmid transfer (tra) region products: traA...
Construction and analysis of F plasmid traR, trbJ, and trbH mutants.
Maneewannakul, K, Ippen-Ihler, K
F plasmid derivatives carrying kan insertion mutations in the transfer region genes traR, trbJ, and trbH were constructed. Standard tests indicated that these loci are not essential for F pilus...
Maneewannakul, S, Maneewannakul, K, Ippen-Ihler, K
The traW gene of the Escherichia coli K-12 sex factor, F, encodes one of the numerous proteins required for conjugative transfer of this plasmid. We have found that the nucleotide sequence of traW...
Characterization of trbC, a new F plasmid tra operon gene that is essential to conjugative transfer.
Maneewannakul, S, Maneewannakul, K, Ippen-Ihler, K
We have characterized a previously unidentified gene, trbC, which is contained in the transfer region of the Escherichia coli K-12 fertility factor, F. Our data show that the trbC gene is located...
Characterization of the F-plasmid conjugative transfer gene traU.
Moore, D, Maneewannakul, K, Maneewannakul, S, Wu, J H, Ippen-Ihler, K, Bradley, D E
We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The...
Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli.
Quinolone-resistant clinical Escherichia coli isolates were examined for mutations in the marRAB operon of the multiple antibiotic resistance (mar) locus. Among 23 strains evaluated, 8 were chosen...
White, D G, Maneewannakul, K, Von Hofe, E, Zillman, M, Eisenberg, W, Field, A K, ...
The multiple antibiotic resistance operon (marORAB) in Escherichia coli controls intrinsic susceptibility and resistance to multiple, structurally different antibiotics and other noxious agents. A...
Characterization of traX, the F plasmid locus required for acetylation of F-pilin subunits.
Maneewannakul, K, Maneewannakul, S, Ippen-Ihler, K
Acetylation of F-pilin subunits has previously been shown to depend upon expression of the F plasmid transfer operon gene traX. To assess the requirement for pilin acetylation in conjugative transfer...
The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.
Moore, D, Hamilton, C M, Maneewannakul, K, Mintz, Y, Frost, L S, Ippen-Ihler, K
The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies...
Maneewannakul, K, Maneewannakul, S, Ippen-Ihler, K
Transfer of the Escherichia coli fertility plasmid, F, is dependent on expression of F pili. Synthesis of F-pilin subunits is known to involve three F plasmid transfer (tra) region products: traA...
Construction and analysis of F plasmid traR, trbJ, and trbH mutants.
Maneewannakul, K, Ippen-Ihler, K
F plasmid derivatives carrying kan insertion mutations in the transfer region genes traR, trbJ, and trbH were constructed. Standard tests indicated that these loci are not essential for F pilus...
Maneewannakul, S, Maneewannakul, K, Ippen-Ihler, K
The traW gene of the Escherichia coli K-12 sex factor, F, encodes one of the numerous proteins required for conjugative transfer of this plasmid. We have found that the nucleotide sequence of traW...
Characterization of trbC, a new F plasmid tra operon gene that is essential to conjugative transfer.
Maneewannakul, S, Maneewannakul, K, Ippen-Ihler, K
We have characterized a previously unidentified gene, trbC, which is contained in the transfer region of the Escherichia coli K-12 fertility factor, F. Our data show that the trbC gene is located...
Characterization of the F-plasmid conjugative transfer gene traU.
Moore, D, Maneewannakul, K, Maneewannakul, S, Wu, J H, Ippen-Ihler, K, Bradley, D E
We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The...