Spaner, D, Migita, K, Ochi, A, Shannon, J, Miller, R G, Pereira, P, ...
To obtain a homogeneous population of gamma delta T cells to investigate their role in an immune response, we have made a scid mouse doubly transgenic for rearranged gamma and delta genes. The...
FK506 augments activation-induced programmed cell death of T lymphocytes in vivo.
Migita, K, Eguchi, K, Kawabe, Y, Tsukada, T, Mizokami, A, Nagataki, S
FK506 is an immunosuppressive drug that inhibits T cell receptor-mediated signal transduction. This drug can induce immunological tolerance in allograft recipients. In this study, we investigated the...
Some KpnI family members are associated with the Alu family in the human genome.
Miyake, T, Migita, K, Sakaki, Y
The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5'...
Spaner, D, Migita, K, Ochi, A, Shannon, J, Miller, R G, Pereira, P, ...
To obtain a homogeneous population of gamma delta T cells to investigate their role in an immune response, we have made a scid mouse doubly transgenic for rearranged gamma and delta genes. The...
FK506 augments activation-induced programmed cell death of T lymphocytes in vivo.
Migita, K, Eguchi, K, Kawabe, Y, Tsukada, T, Mizokami, A, Nagataki, S
FK506 is an immunosuppressive drug that inhibits T cell receptor-mediated signal transduction. This drug can induce immunological tolerance in allograft recipients. In this study, we investigated the...
Some KpnI family members are associated with the Alu family in the human genome.
Miyake, T, Migita, K, Sakaki, Y
The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5'...
Migita, K, Eguchi, K, Kawabe, Y, Nagataki, S
T-cell antigen receptor (TCR), which is not itself a protein tyrosine kinase (PTK), is thought to be associated with at least two SRC-like PTKs, P59fyn and ZAP-70. Activation of these PTKs is...
Migita, K, Eguchi, K, Kawakami, A, Ida, H, Fukuda, T, Kurata, A, ...
The Leu-19 (CD56) antigen, which is recognized by anti-Leu-19 and NKH-1 monoclonal antibody, is a 200,000-220,000 molecular weight (MW) glycoprotein that is expressed predominantly on human natural...
Kita, M, Eguchi, K, Kawabe, Y, Tsukada, T, Migita, K, Kawakami, A, ...
The presence of a putative autoantigen of autoimmune disorder in a target organ may cause accumulation of specific T cells in the inflammatory region. One of the mechanisms of such accumulation...
Migita, K, Eguchi, K, Kawabe, Y, Ichinose, Y, Tsukada, T, Aoyagi, T, ...
Degradation of the extracellular matrix plays an important role in rheumatoid articular destruction. Rheumatoid synovial fibroblasts secrete a large amount of matrix-degrading metalloproteinases...
Eguchi, K, Nakamura, T, Mine, M, Ida, H, Kawakami, A, Migita, K, ...
A T cell line from mononuclear cells in the synovial fluid of a patient with polyarthritis was established. The T cell line reacted with serum samples positive for antibodies to human T cell...
Cytokine production by endothelial cells infected with human T cell lymphotropic virus type I.
Takashima, H, Eguchi, K, Kawakami, A, Kawabe, Y, Migita, K, Sakai, M, ...
OBJECTIVE: To investigate the ability of human T cell lymphotropic virus type I (HTLV-I) to infect endothelial cells and induce cytokine production by these cells. METHODS: Human umbilical vein...
High prevalence of Sjögren's syndrome in patients with HTLV-I associated myelopathy
Nakamura, H., Eguchi, K., Nakamura, T., Mizokami, A., Shirabe, S., Kawakami, A., ...
OBJECTIVE—A high seroprevalence of HTLV-I in female Sjögren's syndrome (SS) patients has been reported in Nagasaki, Japan, an area that is heavily endemic for HTLV-I infection. Salivary IgA class...
Hida, A., Kawabe, Y., Kawakami, A., Migita, K., Tominaga, M., Nakamura, H., ...
OBJECTIVE—To investigate whether Sjögren's syndrome (SS) with anti-HTLV-I antibodies is aetiopathologically distinguishable from SS without these antibodies, the study compared prevalence of...
Polyarteritis nodosa complicated by thrombotic thrombocytopenic purpura
YAMASAKI, S, TOMINAGA, M, KAWAKAMI, A, MIGITA, K, KAWABE, Y, IZUMINO, K, ...
Yamasaki, S, Kawakami, A, Nakashima, T, Nakamura, H, Kamachi, M, Honda, S, ...
OBJECTIVES—To examine whether inhibition of NF-κB induces apoptosis of human synovial cells stimulated by tumour necrosis factor α (TNFα), interleukin 1β (IL1β), and anti-Fas monoclonal...
Origuchi, T, Kawasaki, E, Ide, A, Kamachi, M, Tanaka, F, Ida, H, ...
Kawakami, A, Urayama, S, Yamasaki, S, Hida, A, Miyashita, T, Kamachi, M, ...
Methods: Isolated synovial cells, treated or not with TGFß1, were cultured in the presence or absence of anti-Fas IgM, proteasome inhibitor Z-Leu-Leu-Leu-aldehyde (LLL-CHO), etoposide, or...
Cytokine regulation of HLA on thyroid epithelial cells
Migita, K., Eguchi, K., Otsubo, T., Kawakami, A., Nakao, H., Ueki, Y., ...
The regulation of class I and class II HLA expression in human thyroid follicular cells was studied in vitro. Tumour necrosis factor-alpha (TNF-α) enhanced the expression of class I antigen on...
Cytotoxic activity of interleukin-2 (IL-2) activated killer cells toward thyroid epithelial cells
Migita, K., Eguchi, K., Tezuka, H., Otsubo, T., Kawakami, A., Nakao, H., ...
We investigated the sensitivity of thyroid epithelial cells (thyrocytes) to IL-2 activated killer cells. The thyrocytes were lysed by autologous and allogeneic IL-2-activated killer cells; there were...
Miyashita, T, Kawakami, A, Nakashima, T, Yamasaki, S, Tamai, M, Tanaka, F, ...
We examined the role of osteoprotegerin (OPG) on tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in rheumatoid fibroblast-like synovial cells (FLS). OPG protein...
MIGITA, K, MIYASHITA, T, ISHIBASHI, H, MAEDA, Y, NAKAMURA, M, YATSUHASHI, H, ...
Leflunomide, an isoxazol derivative structurally unrelated to other immunomodulatory drugs, has proven to be efficacious in the treatment of rheumatoid arthritis (RA). This study was conducted to...
Migita, K, Miyashita, T, Maeda, Y, Kimura, H, Nakamura, M, Yatsuhashi, H, ...
Type 1 IFN is thought to be implicated in the autoimmune process of SLE. Plasmacytoid dendric cells (DC), which are natural IFN-α producing cells, play a pivotal epipathogenic role in SLE. The...
Effects of rapamycin on apoptosis of rheumatoid synovial cells
MIGITA, K, EGUCHI, K, ICHINOSE, Y, KAWABE, Y, TSUKADA, T, AOYAGI, T, ...
In the present study, we investigated the effects of an immunosuppressant, rapamycin, on bcl-2 expression and the susceptibility of human rheumatoid synovial fibroblasts to Fas-mediated apoptosis....
Nakamura, H, Koji, T, Tominaga, M, Kawakami, A, Migita, K, Kawabe, Y, ...
Apoptosis is a type of cell death that occurs during morphogenesis and development of the immune system. One of the mechanisms is mediated through the Fas and Fas ligand (FasL) pathway. To determine...
Expression of cyclooxygenase-1 (COX-1) in labial salivary glands of Sjögren's syndrome
Tominaga, M, Migita, K, Sano, H, Fukui, W, Kohno, M, Tsubouchi, Y, ...
COX plays an important role in inflammatory diseases such as rheumatoid arthritis. To determine the role of COX in Sjögren's syndrome (SS), we examined COX expression in the salivary glands of SS...
Impaired degradation of serum amyloid A (SAA) protein by cytokine-stimulated monocytes
Migita, K, Yamasaki, S, Shibatomi, K, Ida, H, Kita, M, Kawakami, A, ...
Secondary amyloidosis (AA amyloidosis) is a systemic disease characterized by the extracellular tissue deposition of insoluble amyloid A (AA) protein. Aberrant metabolism of serum amyloid A (SAA) by...
Expression of membrane-type 1 matrix metalloproteinase in rheumatoid synovial cells
Honda, S, Migita, K, Hirai, Y, Origuchi, T, Yamasaki, S, Kamachi, M, ...
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is thought to be a putative regulator of pro-gelatinase A (MMP-2) in the rheumatoid synovium. In this study, we examined the effects of IL-1β, one...
Regulation of rheumatoid synoviocyte proliferation by endogenous p53 induction
Migita, K, Tanaka, F, Yamasaki, S, Shibatomi, K, Ida, H, Kawakami, A, ...
The p53 tumour suppressor protein protects cells from tumorigenic alterations by inducing either cell growth arrest or apoptosis. In the present study, we investigated the role of endogenous p53...
YAMASAKI, S, NAKASHIMA, T, KAWAKAMI, A, MIYASHITA, T, IDA, H, MIGITA, K, ...
Peroxisome proliferator-activated receptor γ (PPARγ) is a ligand dependent transcriptional factor known to be a regulator of adipogenesis. Recent studies have also shown that stimulation of PPARγ...
MIGITA, K, EGUCHI, K, AOYAGI, T, TSUKADA, T, TSUBOI, M, KAWABE, Y, ...
RA is a chronic inflammatory disease characterized by mononuclear cell infiltration and the overgrowth of synovial fibroblast. This invasive growth of synovial tissues corresponds with the...
Hida, A, Kawakami, A, Nakashima, T, Yamasaki, S, Sakai, H, Urayama, S, ...
Accumulating evidence suggests that macrophages function as major effector cells in the pathological process of various human diseases. We examined here the role of nuclear factor‐κB (NF‐κB)...