Scientific Commons: K. Mihara

The effects of alloying elements on the mechanical properties as well as electrical conductivity in Cu-15%Cr(mass fraction) in-situ composites were systematically studied and high strength and high...

Effect of alloying elements on the mechanical properties in the Cu-15% Cr in-situ composites (2004)

Mihara, K, Ma, J., Sakai, Shigeki, Sun, Shoujin, Suzuki, Hirowo G.

The effects of alloying elements on the mechanical properties as well as electrical conductivity in Cu-15%Cr(mass fraction) in-situ composites were systematically studied and high strength and high...

Effect of alloying elements on the mechanical properties in the Cu-15% Cr in-situ composites (2004)

Mihara, K, Ma, J., Sakai, Shigeki, Sun, Shoujin, Suzuki, Hirowo G.

The effects of alloying elements on the mechanical properties as well as electrical conductivity in Cu-15%Cr(mass fraction) in-situ composites were systematically studied and high strength and high...

Effect of alloying elements on the mechanical properties of Cu-15%Cr in-situ composites (2002)

Ma, J., Mihara, K., Sakai, Shoko, Sun, Shoujin, Suzuki, H.

Abstract not available.

Effect of alloying elements on the mechanical properties of Cu-15%Cr in-situ composites (2002)

Ma, J., Mihara, K., Sakai, Shoko, Sun, Shoujin, Suzuki, H.

Abstract not available.

Effect of alloying elements on the mechanical properties of Cu-15%Cr in-situ composites (2002)

Ma, J., Mihara, K., Sakai, Shoko, Sun, Shoujin, Suzuki, H.

Abstract not available.

Targeting of GRASP65 to the Golgi apparatus (2001)

Yoshimura,S., Misumi,Y., Ikehara,Y., Barr,F. A., Ohno,H., Mihara,K., ...

Targeting of GRASP65 to the Golgi apparatus (2001)

Yoshimura, S., Misumi, Y., Ikehara, Y., Barr, F. A., Ohno, H., Mihara, K., ...

Functions of signal and signal-anchor sequences are determined by the balance between the hydrophobic segment and the N-terminal charge.

Sakaguchi, M, Tomiyoshi, R, Kuroiwa, T, Mihara, K, Omura, T

The signal sequence of secretory proteins and the signal-anchor sequence of type II membrane proteins initiate the translocation of the following polypeptide segments, whereas the signal-anchor...

Trehalose levels and survival ratio of freeze-tolerant versus freeze-sensitive yeasts.

Hino, A, Mihara, K, Nakashima, K, Takano, H

Five freeze-tolerant yeast strains suitable for frozen dough were compared with ordinary commercial bakers' yeast. Kluyveromyces thermotolerans FRI 501 cells showed high survival ability after...

Secondary activation of c-abl may be related to translocation to the nucleolar organizer region in an in vitro cultured rat leukemia cell line (K3D).

Takahashi, R, Mihara, K, Maeda, S, Yamaguchi, T, Chen, H L, Aoyama, N, ...

Localization of cellular oncogenes (c-onc) near the break points of translocations in tumor cells has indicated involvement of these genes in neoplastic growth. Enhanced transcription of the cellular...

Signal recognition particle is required for co-translational insertion of cytochrome P-450 into microsomal membranes.

Sakaguchi, M, Mihara, K, Sato, R

Insertion of newly synthesized P-450(1), the major phenobarbital-inducible form of rabbit liver microsomal cytochrome P-450, into microsomal membranes was studied in a wheat germ cell-free...

In vitro synthesis and integration into mitochondria of porin, a major protein of the outer mitochondrial membrane of Saccharomyces cerevisiae.

Mihara, K, Blobel, G, Sato, R

We have isolated an outer mitochondrial membrane (OMM) fraction from baker's yeast. Saccharomyces cerevisiae, that possesses porin activity and contains a major polypeptide of 29,000 daltons. By...

The four cytoplasmically made subunits of yeast mitochondrial cytochrome c oxidase are synthesized individually and not as a polyprotein.

Mihara, K, Blobel, G

Subunit-specific antisera prepared against each of the four cytoplasmically made subunits (IV, V, VI, and VII) of yeast mitochondrial cytochrome c oxidase (EC 1.9.3.1) were used to precipitate...

Antimicrobial Properties of Natural Phenols and Related Compounds: I. Obtusastyrene

Jurd, L., King, A. D., Mihara, K., Stanley, W. L.

Obtusastyrene (4-cinnamylphenol) displays effective antimicrobial activity in vitro against a variety of gram-positive bacteria, yeasts, and molds. The activity of obtusastyrene is not appreciably...

MSF, a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria.

Hachiya, N, Komiya, T, Alam, R, Iwahashi, J, Sakaguchi, M, Omura, T, ...

Mitochondrial import stimulation factor (MSF) unfolds wheat germ lysate synthesized aggregated mitochondrial precursor proteins and stimulates their mitochondrial import in an ATP dependent manner....

A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins.

Hachiya, N, Alam, R, Sakasegawa, Y, Sakaguchi, M, Mihara, K, Omura, T

Rat liver cytosol contained an activity that stimulated the import of wheat germ lysate-synthesized precursor proteins into mitochondria. The activity was purified 10,000-fold from the cytosol as a...

Cytoplasmic chaperones determine the targeting pathway of precursor proteins to mitochondria.

Komiya, T, Sakaguchi, M, Mihara, K

Two ATP-dependent cytosolic chaperones, mitochondrial import stimulation factor (MSF) and hsp70, are known to be involved in the import of precursor proteins into mitochondria. Hsp70 generally...

Cytosolic and mitochondrial surface factor-independent import of a synthetic peptide into mitochondria.

Furuya, S, Mihara, K, Aimoto, S, Omura, T

We chemically synthesized a peptide, 11 beta-45, which was composed of 45 amino acid residues including the whole extension peptide and some of the mature portion of bovine cytochrome P-450(11 beta)...

The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane.

Sato, T, Sakaguchi, M, Mihara, K, Omura, T

We previously showed that the amino-terminal region of P-450 is responsible not only for targeting to endoplasmic reticulum (ER) membrane but also for stable anchoring to the membrane. In the present...

A short amino-terminal segment of microsomal cytochrome P-450 functions both as an insertion signal and as a stop-transfer sequence.

Sakaguchi, M, Mihara, K, Sato, R

Co-translational insertion of liver microsomal cytochrome P-450 into the endoplasmic reticulum membrane is mediated by the signal recognition particle (SRP) and the presence in the cytochrome...

Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure.

Mihara, K, Sato, R

We have cloned a full-length cDNA for yeast porin, the major outer mitochondrial membrane protein from Saccharomyces cerevisiae, and determined its nucleotide sequence. The primary structure of the...

Binding of mitochondrial precursor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones.

Komiya, T, Rospert, S, Schatz, G, Mihara, K

We have reconstituted the early steps of precursor targeting to mitochondria in a defined and soluble system consisting of the cytosolic domains of the yeast mitochondrial import receptors Tom20 and...

Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway: evidence for the 'acid chain' hypothesis.

Komiya, T, Rospert, S, Koehler, C, Looser, R, Schatz, G, Mihara, K

Mitochondrial precursor proteins with basic targeting signals may be transported across the outer membrane by sequential binding to acidic receptor sites of increasing affinity. To test this 'acid...

Functions of signal and signal-anchor sequences are determined by the balance between the hydrophobic segment and the N-terminal charge.

Sakaguchi, M, Tomiyoshi, R, Kuroiwa, T, Mihara, K, Omura, T

The signal sequence of secretory proteins and the signal-anchor sequence of type II membrane proteins initiate the translocation of the following polypeptide segments, whereas the signal-anchor...

Trehalose levels and survival ratio of freeze-tolerant versus freeze-sensitive yeasts.

Hino, A, Mihara, K, Nakashima, K, Takano, H

Five freeze-tolerant yeast strains suitable for frozen dough were compared with ordinary commercial bakers' yeast. Kluyveromyces thermotolerans FRI 501 cells showed high survival ability after...

Secondary activation of c-abl may be related to translocation to the nucleolar organizer region in an in vitro cultured rat leukemia cell line (K3D).

Takahashi, R, Mihara, K, Maeda, S, Yamaguchi, T, Chen, H L, Aoyama, N, ...

Localization of cellular oncogenes (c-onc) near the break points of translocations in tumor cells has indicated involvement of these genes in neoplastic growth. Enhanced transcription of the cellular...

Signal recognition particle is required for co-translational insertion of cytochrome P-450 into microsomal membranes.

Sakaguchi, M, Mihara, K, Sato, R

Insertion of newly synthesized P-450(1), the major phenobarbital-inducible form of rabbit liver microsomal cytochrome P-450, into microsomal membranes was studied in a wheat germ cell-free...

In vitro synthesis and integration into mitochondria of porin, a major protein of the outer mitochondrial membrane of Saccharomyces cerevisiae.

Mihara, K, Blobel, G, Sato, R

We have isolated an outer mitochondrial membrane (OMM) fraction from baker's yeast. Saccharomyces cerevisiae, that possesses porin activity and contains a major polypeptide of 29,000 daltons. By...

The four cytoplasmically made subunits of yeast mitochondrial cytochrome c oxidase are synthesized individually and not as a polyprotein.

Mihara, K, Blobel, G

Subunit-specific antisera prepared against each of the four cytoplasmically made subunits (IV, V, VI, and VII) of yeast mitochondrial cytochrome c oxidase (EC 1.9.3.1) were used to precipitate...

Antimicrobial Properties of Natural Phenols and Related Compounds: I. Obtusastyrene

Jurd, L., King, A. D., Mihara, K., Stanley, W. L.

Obtusastyrene (4-cinnamylphenol) displays effective antimicrobial activity in vitro against a variety of gram-positive bacteria, yeasts, and molds. The activity of obtusastyrene is not appreciably...

MSF, a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria.

Hachiya, N, Komiya, T, Alam, R, Iwahashi, J, Sakaguchi, M, Omura, T, ...

Mitochondrial import stimulation factor (MSF) unfolds wheat germ lysate synthesized aggregated mitochondrial precursor proteins and stimulates their mitochondrial import in an ATP dependent manner....

A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins.

Hachiya, N, Alam, R, Sakasegawa, Y, Sakaguchi, M, Mihara, K, Omura, T

Rat liver cytosol contained an activity that stimulated the import of wheat germ lysate-synthesized precursor proteins into mitochondria. The activity was purified 10,000-fold from the cytosol as a...

Cytoplasmic chaperones determine the targeting pathway of precursor proteins to mitochondria.

Komiya, T, Sakaguchi, M, Mihara, K

Two ATP-dependent cytosolic chaperones, mitochondrial import stimulation factor (MSF) and hsp70, are known to be involved in the import of precursor proteins into mitochondria. Hsp70 generally...

Cytosolic and mitochondrial surface factor-independent import of a synthetic peptide into mitochondria.

Furuya, S, Mihara, K, Aimoto, S, Omura, T

We chemically synthesized a peptide, 11 beta-45, which was composed of 45 amino acid residues including the whole extension peptide and some of the mature portion of bovine cytochrome P-450(11 beta)...

The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane.

Sato, T, Sakaguchi, M, Mihara, K, Omura, T

We previously showed that the amino-terminal region of P-450 is responsible not only for targeting to endoplasmic reticulum (ER) membrane but also for stable anchoring to the membrane. In the present...

A short amino-terminal segment of microsomal cytochrome P-450 functions both as an insertion signal and as a stop-transfer sequence.

Sakaguchi, M, Mihara, K, Sato, R

Co-translational insertion of liver microsomal cytochrome P-450 into the endoplasmic reticulum membrane is mediated by the signal recognition particle (SRP) and the presence in the cytochrome...

Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure.

Mihara, K, Sato, R

We have cloned a full-length cDNA for yeast porin, the major outer mitochondrial membrane protein from Saccharomyces cerevisiae, and determined its nucleotide sequence. The primary structure of the...

Binding of mitochondrial precursor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones.

Komiya, T, Rospert, S, Schatz, G, Mihara, K

We have reconstituted the early steps of precursor targeting to mitochondria in a defined and soluble system consisting of the cytosolic domains of the yeast mitochondrial import receptors Tom20 and...

Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway: evidence for the 'acid chain' hypothesis.

Komiya, T, Rospert, S, Koehler, C, Looser, R, Schatz, G, Mihara, K

Mitochondrial precursor proteins with basic targeting signals may be transported across the outer membrane by sequential binding to acidic receptor sites of increasing affinity. To test this 'acid...

C-myc expression in lymphocytes of MRL/MP-lpr mice activated by A23187 and TPA.

Koizumi, T, Nakao, Y, Matsui, T, Katakami, Y, Mihara, K, Takahashi, R, ...

In order to analyse the molecular mechanism of lymphocyte activation defect in MRL/MP-lpr (MRL/1) mice, c-myc proto-oncogene expression was examined in MRL/1 lymph node cells stimulated by various...

K. Mihara

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