Clubb, R T, Mizuuchi, M, Huth, J R, Omichinski, J G, Savilahti, H, Mizuuchi, K, ...
A tetramer of the Mu transposase (MuA) pairs the recombination sites, cleaves the donor DNA, and joins these ends to a target DNA by strand transfer. Juxtaposition of the recombination sites is...
DNase protection analysis of the stable synaptic complexes involved in Mu transposition.
Mizuuchi, M, Baker, T A, Mizuuchi, K
Several critical steps in phage Mu transposition involve specialized protein-DNA complexes. Cleavage of Mu donor DNA by MuA protein leads to the formation of the stable cleaved donor complex (CDC),...
Lymphoid V(D)J recombination: nucleotide insertion at signal joints as well as coding joints.
Lieber, M R, Hesse, J E, Mizuuchi, K, Gellert, M
The coding regions of antigen receptor genes assembled by variable-diversity-joining region [V(D)J] recombination are known in many cases to have undergone deletions of several nucleotides and also...
Maxwell, A, Craigie, R, Mizuuchi, K
A DNA strand-transfer reaction is an early step in the transposition of phage Mu. It has been shown that an efficient reaction in vitro requires, in addition to buffer and salt, only the Mu A...
Site-specific interaction of DNA gyrase with DNA.
Fisher, L M, Mizuuchi, K, O'Dea, M H, Ohmori, H, Gellert, M
DNA gyrase, in the presence of the inhibitor oxolinic acid, can induce double-strand DNA breakage at specific sites. The sequences at several sites have been determined. In addition, the structure of...
DNA sequence and transcription of the region upstream of the E. coli gyrB gene.
Adachi, T, Mizuuchi, K, Menzel, R, Gellert, M
We have determined the sequence of a 1498 base-pair region in E. coli that extends from within dnaN through recF and into the gyrB gene. An open reading frame of 1071 base pairs has been identified...
A rapid in vitro assay for HIV DNA integration.
Craigie, R, Mizuuchi, K, Bushman, F D, Engelman, A
Retroviruses synthesize a double stranded DNA copy of their RNA genome after infection of a permissive cell and subsequent integration of this DNA copy into the host genome is necessary for normal...
Mizuuchi, M, Weisberg, R A, Mizuuchi, K
We have determined the DNA sequence of the control region of phage D108 up to position 1419 at the left end of the phage genome. Open reading frames for the repressor gene, ner gene, and the 5' part...
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
Adachi, T, Mizuuchi, M, Robinson, E A, Appella, E, O'Dea, M H, Gellert, M, ...
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide...
The extent of DNA sequence required for a functional bacterial attachment site of phage lambda.
We have investigated the extent of DNA sequence required to form a bacterial attachment site (attB) that functions in bacteriophage lambda integration. A DNA fragment carrying attB of Escherichia...
DNA gyrase action involves the introduction of transient double-strand breaks into DNA.
Mizuuchi, K, Fisher, L M, O'Dea, M H, Gellert, M
DNA gyrase from Escherichia coli, in the presence of ATP, can both separate catenated DNA circles and unknot knotted DNA. Both these reactions require passage of a DNA segment through a transient...
We have investigated the minimum extent of DNA sequence required for the attachment site of bacteriophage lambda to function in integrative recombination. A DNA fragment carrying the phage attachment...
Slow cruciform transitions in palindromic DNA.
Gellert, M, O'Dea, M H, Mizuuchi, K
Extrusion of cruciform structures in self-complementary regions of DNA is known to be favored by negative supercoiling of DNA. We show here that, in moderately supercoiled DNA, cruciform extrusion is...
Craigie, R, Arndt-Jovin, D J, Mizuuchi, K
An early step in the transposition of bacteriophage Mu DNA in vitro is a DNA strand-transfer reaction that generates an intermediate DNA structure in which the Mu donor DNA and the target DNA are...
DNA gyrase: subunit structure and ATPase activity of the purified enzyme.
Mizuuchi, K, O'Dea, M H, Gellert, M
DNA gyrase has been purified to near homogeneity from Escherichia coli. The enzyme consists of two subunits of molecular weights 90,000 and 100,000 present in roughly equimolar amounts. The subunits...
The phage Mu transpososome core: DNA requirements for assembly and function.
Savilahti, H, Rice, P A, Mizuuchi, K
The two chemical steps of phage Mu transpositional recombination, donor DNA cleavage and strand transfer, take place within higher order protein-DNA complexes called transpososomes. At the core of...
A novel assay has been developed for in vitro genetic recombination of DNA. Substrate and product DNAs are cleaved with a restriction endonuclease and the resulting fragments are separated by...
DNA gyrase: an enzyme that introduces superhelical turns into DNA.
Gellert, M, Mizuuchi, K, O'Dea, M H, Nash, H A
Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated...
A large nucleoprotein assembly at the ends of the viral DNA mediates retroviral DNA integration.
Wei, S Q, Mizuuchi, K, Craigie, R
We have probed the nucleoprotein organization of Moloney murine leukemia virus (MLV) pre-integration complexes using a novel footprinting technique that utilizes a simplified in vitro phage Mu...
Schumacher, S, Clubb, R T, Cai, M, Mizuuchi, K, Clore, G M, Gronenborn, A M
The phage Mu transposase (MuA) binds to the ends of the Mu genome during the assembly of higher order nucleoprotein complexes. We investigate the structure and function of the MuA end-binding domain...
Clubb, R T, Mizuuchi, M, Huth, J R, Omichinski, J G, Savilahti, H, Mizuuchi, K, ...
A tetramer of the Mu transposase (MuA) pairs the recombination sites, cleaves the donor DNA, and joins these ends to a target DNA by strand transfer. Juxtaposition of the recombination sites is...
DNase protection analysis of the stable synaptic complexes involved in Mu transposition.
Mizuuchi, M, Baker, T A, Mizuuchi, K
Several critical steps in phage Mu transposition involve specialized protein-DNA complexes. Cleavage of Mu donor DNA by MuA protein leads to the formation of the stable cleaved donor complex (CDC),...
Lymphoid V(D)J recombination: nucleotide insertion at signal joints as well as coding joints.
Lieber, M R, Hesse, J E, Mizuuchi, K, Gellert, M
The coding regions of antigen receptor genes assembled by variable-diversity-joining region [V(D)J] recombination are known in many cases to have undergone deletions of several nucleotides and also...
Maxwell, A, Craigie, R, Mizuuchi, K
A DNA strand-transfer reaction is an early step in the transposition of phage Mu. It has been shown that an efficient reaction in vitro requires, in addition to buffer and salt, only the Mu A...
Site-specific interaction of DNA gyrase with DNA.
Fisher, L M, Mizuuchi, K, O'Dea, M H, Ohmori, H, Gellert, M
DNA gyrase, in the presence of the inhibitor oxolinic acid, can induce double-strand DNA breakage at specific sites. The sequences at several sites have been determined. In addition, the structure of...
DNA sequence and transcription of the region upstream of the E. coli gyrB gene.
Adachi, T, Mizuuchi, K, Menzel, R, Gellert, M
We have determined the sequence of a 1498 base-pair region in E. coli that extends from within dnaN through recF and into the gyrB gene. An open reading frame of 1071 base pairs has been identified...
A rapid in vitro assay for HIV DNA integration.
Craigie, R, Mizuuchi, K, Bushman, F D, Engelman, A
Retroviruses synthesize a double stranded DNA copy of their RNA genome after infection of a permissive cell and subsequent integration of this DNA copy into the host genome is necessary for normal...
Mizuuchi, M, Weisberg, R A, Mizuuchi, K
We have determined the DNA sequence of the control region of phage D108 up to position 1419 at the left end of the phage genome. Open reading frames for the repressor gene, ner gene, and the 5' part...
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
Adachi, T, Mizuuchi, M, Robinson, E A, Appella, E, O'Dea, M H, Gellert, M, ...
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide...
The extent of DNA sequence required for a functional bacterial attachment site of phage lambda.
We have investigated the extent of DNA sequence required to form a bacterial attachment site (attB) that functions in bacteriophage lambda integration. A DNA fragment carrying attB of Escherichia...
DNA gyrase action involves the introduction of transient double-strand breaks into DNA.
Mizuuchi, K, Fisher, L M, O'Dea, M H, Gellert, M
DNA gyrase from Escherichia coli, in the presence of ATP, can both separate catenated DNA circles and unknot knotted DNA. Both these reactions require passage of a DNA segment through a transient...
We have investigated the minimum extent of DNA sequence required for the attachment site of bacteriophage lambda to function in integrative recombination. A DNA fragment carrying the phage attachment...
Slow cruciform transitions in palindromic DNA.
Gellert, M, O'Dea, M H, Mizuuchi, K
Extrusion of cruciform structures in self-complementary regions of DNA is known to be favored by negative supercoiling of DNA. We show here that, in moderately supercoiled DNA, cruciform extrusion is...
Craigie, R, Arndt-Jovin, D J, Mizuuchi, K
An early step in the transposition of bacteriophage Mu DNA in vitro is a DNA strand-transfer reaction that generates an intermediate DNA structure in which the Mu donor DNA and the target DNA are...
DNA gyrase: subunit structure and ATPase activity of the purified enzyme.
Mizuuchi, K, O'Dea, M H, Gellert, M
DNA gyrase has been purified to near homogeneity from Escherichia coli. The enzyme consists of two subunits of molecular weights 90,000 and 100,000 present in roughly equimolar amounts. The subunits...
The phage Mu transpososome core: DNA requirements for assembly and function.
Savilahti, H, Rice, P A, Mizuuchi, K
The two chemical steps of phage Mu transpositional recombination, donor DNA cleavage and strand transfer, take place within higher order protein-DNA complexes called transpososomes. At the core of...
A novel assay has been developed for in vitro genetic recombination of DNA. Substrate and product DNAs are cleaved with a restriction endonuclease and the resulting fragments are separated by...
DNA gyrase: an enzyme that introduces superhelical turns into DNA.
Gellert, M, Mizuuchi, K, O'Dea, M H, Nash, H A
Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated...
A large nucleoprotein assembly at the ends of the viral DNA mediates retroviral DNA integration.
Wei, S Q, Mizuuchi, K, Craigie, R
We have probed the nucleoprotein organization of Moloney murine leukemia virus (MLV) pre-integration complexes using a novel footprinting technique that utilizes a simplified in vitro phage Mu...
Schumacher, S, Clubb, R T, Cai, M, Mizuuchi, K, Clore, G M, Gronenborn, A M
The phage Mu transposase (MuA) binds to the ends of the Mu genome during the assembly of higher order nucleoprotein complexes. We investigate the structure and function of the MuA end-binding domain...