In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs.
McDonald, V, Stables, R, Warhurst, D C, Barer, M R, Blewett, D A, Chapman, H D, ...
Sporozoites of Cryptosporidium parvum which were excysted in vitro from oocysts isolated from calves or patients with acquired immune deficiency syndrome underwent development in monolayers of the...
Induction of Th1 cytokine responses by mycobacterial antigens in leprosy.
Dockrell, H M, Young, S K, Britton, K, Brennan, P J, Rivoire, B, Waters, M F, ...
Twelve mycobacterial antigens were compared for induction of gamma interferon (IFN-gamma) secretion by human blood mononuclear cells of patients with leprosy. Fractionated Mycobacterium leprae...
Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species.
Nina, J M, McDonald, V, Dyson, D A, Catchpole, J, Uni, S, Iseki, M, ...
A comparison was made of the antigenic composition of oocyst walls and sporozoites from Cryptosporidium baileyi from turkeys, C. muris from rodents, and C. parvum from ruminants, employing...
Khanolkar-Young, S, Kolk, A H, Andersen, A B, Bennedsen, J, Brennan, P J, Rivoire, B, ...
An international workshop was sponsored by the World Health organization to screen new antimycobacterial monoclonal antibodies and to identify antibodies which could be recommended as standard...
Tobias, P S, McAdam, K P, Soldau, K, Ulevitch, R J
We have recently described several phenomena involving the interactions of lipopolysaccharides (LPS) from Salmonella minnesota Re595 (Re595-LPS) with rabbit serum, which are different in and unique...
Recognition of mycobacterial antigens by sera from patients with leprosy.
Vega-Lopez, F, Stoker, N G, Locniskar, M F, Dockrell, H M, Grant, K A, McAdam, K P
Mycobacterium leprae sonic extracts prepared from armadillo-derived bacteria were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting)...
Gillespie, S H, McWhinney, P H, Patel, S, Raynes, J G, McAdam, K P, Whiley, R A, ...
An enzyme-linked immunosorbent assay technique was used to detect and quantify C-polysaccharide-like antigen in strains of alpha-hemolytic streptococci classified into species by following the latest...
Cellular immune responses of leprosy contacts to fractionated Mycobacterium leprae antigens.
Lee, S P, Stoker, N G, Grant, K A, Handzel, Z T, Hussain, R, McAdam, K P, ...
Antigens of armadillo-derived Mycobacterium leprae sonic extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane, and the...
T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae.
Dockrell, H M, Stoker, N G, Lee, S P, Jackson, M, Grant, K A, Jouy, N F, ...
The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of...
McAdam, K P, Foss, N T, Garcia, C, DeLellis, R, Chedid, L, Rees, R J, ...
Serum amyloid A protein (SAA) elevation accompanies induction of secondary amyloidosis in mice given Mycobacterium butyricum in Freund adjuvant. The synthesis of SAA by cultured hepatocytes is...
Measurement of acute phase proteins for assessing severity of Plasmodium falciparum malaria.
Gillespie, S H, Dow, C, Raynes, J G, Behrens, R H, Chiodini, P L, McAdam, K P
Seventeen adult patients with acute Plasmodium falciparum malaria, admitted to the Hospital for Tropical Diseases, were studied. Serial measurements of the serum concentration of C-reactive protein,...
Gillespie, S H, Smith, M D, Dickens, A, Raynes, J G, McAdam, K P
AIMS--To evaluate the use of a quantitative enzyme linked immunosorbent assay (ELISA) detecting C-polysaccharide (PnC) antigen in sputum for the diagnosis of Streptococcus pneumoniae infection....
Detection of C-polysaccharide in serum of patients with Streptococcus pneumoniae bacteraemia.
Gillespie, S H, Smith, M D, Dickens, A, Raynes, J G, McAdam, K P
AIM--To investigate the fate of Streptococcus pneumoniae C-polysaccharide antigen in serum in patients with S pneumoniae bacteraemia. METHOD--In vitro dissociation experiments were performed to...
Lymphoid cells in endotoxin-induced production of the amyloid-related serum amyloid A protein.
Rosenstreich, D L, McAdam, K P
Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid...
Joiner, K A, McAdam, K P, Kasper, D L
The lipopolysaccharides (LPS) from Bacteroides fragilis are structurally atypical and give weak responses in most tests of endotoxic activity, but the mitogenic activity of LPS from B. fragilis has...
Impact of HIV on tuberculosis in Zambia: a cross sectional study.
Elliott, A M, Luo, N, Tembo, G, Halwiindi, B, Steenbergen, G, Machiels, L, ...
OBJECTIVE--To examine the contribution of HIV infection to the apparently increasing incidence of tuberculosis in central Africa. DESIGN--Cross sectional study. SETTING--Outpatient clinic in teaching...
In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs.
McDonald, V, Stables, R, Warhurst, D C, Barer, M R, Blewett, D A, Chapman, H D, ...
Sporozoites of Cryptosporidium parvum which were excysted in vitro from oocysts isolated from calves or patients with acquired immune deficiency syndrome underwent development in monolayers of the...
Induction of Th1 cytokine responses by mycobacterial antigens in leprosy.
Dockrell, H M, Young, S K, Britton, K, Brennan, P J, Rivoire, B, Waters, M F, ...
Twelve mycobacterial antigens were compared for induction of gamma interferon (IFN-gamma) secretion by human blood mononuclear cells of patients with leprosy. Fractionated Mycobacterium leprae...
Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species.
Nina, J M, McDonald, V, Dyson, D A, Catchpole, J, Uni, S, Iseki, M, ...
A comparison was made of the antigenic composition of oocyst walls and sporozoites from Cryptosporidium baileyi from turkeys, C. muris from rodents, and C. parvum from ruminants, employing...
Khanolkar-Young, S, Kolk, A H, Andersen, A B, Bennedsen, J, Brennan, P J, Rivoire, B, ...
An international workshop was sponsored by the World Health organization to screen new antimycobacterial monoclonal antibodies and to identify antibodies which could be recommended as standard...
Tobias, P S, McAdam, K P, Soldau, K, Ulevitch, R J
We have recently described several phenomena involving the interactions of lipopolysaccharides (LPS) from Salmonella minnesota Re595 (Re595-LPS) with rabbit serum, which are different in and unique...
Recognition of mycobacterial antigens by sera from patients with leprosy.
Vega-Lopez, F, Stoker, N G, Locniskar, M F, Dockrell, H M, Grant, K A, McAdam, K P
Mycobacterium leprae sonic extracts prepared from armadillo-derived bacteria were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting)...
Gillespie, S H, McWhinney, P H, Patel, S, Raynes, J G, McAdam, K P, Whiley, R A, ...
An enzyme-linked immunosorbent assay technique was used to detect and quantify C-polysaccharide-like antigen in strains of alpha-hemolytic streptococci classified into species by following the latest...
Cellular immune responses of leprosy contacts to fractionated Mycobacterium leprae antigens.
Lee, S P, Stoker, N G, Grant, K A, Handzel, Z T, Hussain, R, McAdam, K P, ...
Antigens of armadillo-derived Mycobacterium leprae sonic extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane, and the...
T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae.
Dockrell, H M, Stoker, N G, Lee, S P, Jackson, M, Grant, K A, Jouy, N F, ...
The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of...
McAdam, K P, Foss, N T, Garcia, C, DeLellis, R, Chedid, L, Rees, R J, ...
Serum amyloid A protein (SAA) elevation accompanies induction of secondary amyloidosis in mice given Mycobacterium butyricum in Freund adjuvant. The synthesis of SAA by cultured hepatocytes is...
Measurement of acute phase proteins for assessing severity of Plasmodium falciparum malaria.
Gillespie, S H, Dow, C, Raynes, J G, Behrens, R H, Chiodini, P L, McAdam, K P
Seventeen adult patients with acute Plasmodium falciparum malaria, admitted to the Hospital for Tropical Diseases, were studied. Serial measurements of the serum concentration of C-reactive protein,...
Gillespie, S H, Smith, M D, Dickens, A, Raynes, J G, McAdam, K P
AIMS--To evaluate the use of a quantitative enzyme linked immunosorbent assay (ELISA) detecting C-polysaccharide (PnC) antigen in sputum for the diagnosis of Streptococcus pneumoniae infection....
Detection of C-polysaccharide in serum of patients with Streptococcus pneumoniae bacteraemia.
Gillespie, S H, Smith, M D, Dickens, A, Raynes, J G, McAdam, K P
AIM--To investigate the fate of Streptococcus pneumoniae C-polysaccharide antigen in serum in patients with S pneumoniae bacteraemia. METHOD--In vitro dissociation experiments were performed to...
Lymphoid cells in endotoxin-induced production of the amyloid-related serum amyloid A protein.
Rosenstreich, D L, McAdam, K P
Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid...
Joiner, K A, McAdam, K P, Kasper, D L
The lipopolysaccharides (LPS) from Bacteroides fragilis are structurally atypical and give weak responses in most tests of endotoxic activity, but the mitogenic activity of LPS from B. fragilis has...
Impact of HIV on tuberculosis in Zambia: a cross sectional study.
Elliott, A M, Luo, N, Tembo, G, Halwiindi, B, Steenbergen, G, Machiels, L, ...
OBJECTIVE--To examine the contribution of HIV infection to the apparently increasing incidence of tuberculosis in central Africa. DESIGN--Cross sectional study. SETTING--Outpatient clinic in teaching...
Locniskar, M, Zumla, A, Mudd, D W, Isenberg, D A, Williams, W, McAdam, K P
Human monoclonal antibodies (mAb) were produced by hybridomas derived from fusion of the GM4672 lymphoblastoid cell line and peripheral blood mononuclear cells from leprosy patients. Hybridoma...
Gauldie, J, Sauder, D N, McAdam, K P, Dinarello, C A
A universal component of inflammation is the increased synthesis of a series of plasma proteins (acute-phase proteins) by the liver. The postulated messenger of acute-phase protein induction is...
The acute phase response in parasite infection. Nippostrongylus brasiliensis in the mouse.
Lamontagne, L R, Gauldie, J, Befus, A D, McAdam, K P, Baltz, M L, Pepys, M B
Systemic inflammatory reactions are a prominent feature of many parasitic infections and the cellular and humoral components of the acute phase reaction may have an impact on the host-parasite...
Autoantibodies to cerebroside sulphate (sulphatide) in leprosy.
Wheeler, P R, Raynes, J G, McAdam, K P
Sera from 40 leprosy patients were screened for autoantibodies to cerebroside sulphate (sulphatide). Anti-sulphatide IgM in groups of patients with lepromatous (LL) and borderline (BL + BB + BT), but...
Raynes, J G, Eagling, S, McAdam, K P
Interleukin-6 (IL-6, BSF-2 or IFN-beta 2) is thought to be the major regulator of the acute-phase protein response that follows tissue injury and inflammation, with interleukin-1 (IL-1), tumour...
Expression of a common idiotype PR4 in the sera of patients with leprosy.
Zumla, A, Williams, W, Mudd, D, Locniskar, M, Behrens, R, Isenberg, D, ...
The sera of 187 patients from across the leprosy spectrum were screened for the expression of the PR4 idiotype, which was first identified on a human hybridoma-derived monoclonal antibody from a...
Amyloid fibril protein AA in Papua New Guinean amyloidosis.
Anders, R F, Price, M A, Wilkey, I S, Husby, G, Takitaki, F, Natvig, J B, ...
In this study of protein composition of amyloid fibrils isolated from eight patients representative of the spectrum of amyloidosis found in Papua New Guinea has been investigated. All fibril...
Cell-mediated immunity in amyloidosis secondary to lepromatous leprosy.
Anders, E M, McAdam, K P, Anders, R F
Cell-mediated immunity in lepromatous leprosy patients with and without amyloidosis has been studied. Amyloidosis occurred mostly in patients with a history of recurrent erythema nodosum leprosum...
Roberts, P P, Dockrell, H M, McAdam, K P
A positive Mitsuda skin test for delayed type hypersensitivity to Mycobacterium leprae is associated with a high level of protection against lepromatous leprosy, while the value of tuberculin...
Serum lymphocytotoxic activity in leprosy.
Rasheed, F N, Locniskar, M, McCloskey, D J, Hasan, R S, Chiang, T J, Rose, P, ...
Sera from 167 patients across the spectrum of leprosy and 46 endemic controls were screened for lymphocytotoxic activity (LCA). The Terasaki microdroplet lymphocytotoxicity assay was performed at 37...
Da Costa, C T, Khanolkar-Young, S, Elliott, A M, Wasunna, K M, McAdam, K P
Immunoglobulin G subclass responses to lipoarabinomannan (LAM) of Mycobacterium tuberculosis were determined by ELISA in both HIV-1 antibody positive (n = 31) and negative (n = 43) patients with...
Mycobacterium tuberculosis genome-wide screen exposes multiple CD8+ T cell epitopes
Hammond, A S, Klein, M R, Corrah, T, Fox, A, Jaye, A, McAdam, K P, ...
Mounting evidence suggests human leucocyte antigen (HLA) class I-restricted CD8+ T cells play a role in protective immunity against tuberculosis yet relatively few epitopes specific for the causative...