Thymosin β4 induces adult epicardial progenitor mobilization and neovascularization (2007)
Smart, N., Risebro, C.A., Melville, A.A.D., Moses, K., Schwartz, R.J., Chien, K.R., ...
Cardiac failure has a principal underlying aetiology of ischaemic damage arising from vascular insufficiency. Molecules that regulate collateral growth in the ischaemic heart also regulate coronary...
Knoll, R., Postel, R., Wang, J., Kratzner, R., Hennecke, G., Vacaru, A. M., ...
BACKGROUND: Extracellular matrix proteins, such as laminins, and endothelial cells are known to influence cardiomyocyte performance; however, the underlying molecular mechanisms remain poorly...
Absence epilepsy and sinus dysrhythmia in mice lacking the pacemaker channel HCN2 (2003)
Ludwig, A., Budde, T., Stieber, J., Moosmang, S., Wahl, C., Holthoff, K., ...
Dyson, E, Sucov, H M, Kubalak, S W, Schmid-Schönbein, G W, DeLano, F A, Evans, R M, ...
We have recently characterized a cardiac model of ventricular chamber defects in retinoid X receptor alpha (RXR alpha) homozygous mutant (-/-) gene-targeted mice. These mice display generalized...
Retinoid-dependent pathways suppress myocardial cell hypertrophy.
Zhou, M D, Sucov, H M, Evans, R M, Chien, K R
Utilizing an in vitro model system of cardiac muscle cell hypertrophy, we have identified a retinoic acid (RA)-mediated pathway that suppresses the acquisition of specific features of the...
Expression cloning of cardiotrophin 1, a cytokine that induces cardiac myocyte hypertrophy.
Pennica, D, King, K L, Shaw, K J, Luis, E, Rullamas, J, Luoh, S M, ...
Heart failure continues to be a leading cause of mortality worldwide. A hallmark of this disease is dilated cardiac hypertrophy, which is accompanied by a reactivation of genes expressed in fetal...
Molecular and physiological alterations in murine ventricular dysfunction.
Rockman, H A, Ono, S, Ross, R S, Jones, L R, Karimi, M, Bhargava, V, ...
The present study reports the development and characterization of a murine model of right ventricular dysfunction following graded constriction in the pulmonary artery via microsurgical approaches....
O'Brien, T X, Lee, K J, Chien, K R
To study the process of ventricular specification during cardiogenesis, we examined the in situ expression of cardiac ventricular myosin light chain 2 (MLC-2v) mRNA during murine embryogenesis. As...
Shubeita, H E, Martinson, E A, Van Bilsen, M, Chien, K R, Brown, J H
A cultured myocardial cell model was used to examine the role of protein kinase C-dependent pathways in the transcriptional activation of two cardiac muscle genes [myosin light chain 2 (MLC-2) and...
Rockman, H A, Ross, R S, Harris, A N, Knowlton, K U, Steinhelper, M E, Field, L J, ...
To study the mechanisms that activate expression of the atrial natriuretic factor (ANF) gene during pressure-induced hypertrophy, we have developed and characterized an in vivo murine model of...
Knowlton, K U, Rockman, H A, Itani, M, Vovan, A, Seidman, C E, Chien, K R
To determine whether similar or divergent pathways mediate atrial natriuretic factor (ANF) induction in neonatal and hypertrophied adult ventricular myocardium, and to assess whether studies using an...
Transient assays in cultured ventricular muscle cells and studies in transgenic mice have identified two adjacent regulatory elements (HF-1a and HF-1b/MEF-2) as required to maintain ventricular...
Feldman, S T, Gjerset, R, Gately, D, Chien, K R, Feramisco, J R
Infection with the Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial cells. Expression of large T antigen occurred...
Duerr, R L, Huang, S, Miraliakbar, H R, Clark, R, Chien, K R, Ross, J
To determine whether additional hypertrophy would be beneficial or maladaptive in cardiac failure, the effects of insulin-like growth factor (IGF-1) were investigated in rats with left ventricular...
Thorburn, J, Carlson, M, Mansour, S J, Chien, K R, Ahn, N G, Thorburn, A
Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated...
Sills, M N, Xu, Y C, Baracchini, E, Goodman, R H, Cooperman, S S, Mandel, G, ...
This study examined the diversity of Na+ channel gene expression in intact cardiac tissue and purified myocardial cells. The screening of neonatal rat myocardial cell cDNA libraries with a conserved...
Lee, K J, Hickey, R, Zhu, H, Chien, K R
The cardiac myosin light-chain 2v (MLC-2v) gene has served as a model system to identify the pathways which restrict the expression of cardiac muscle genes to particular chambers of the heart during...
Evans, S M, Tai, L J, Tan, V P, Newton, C B, Chien, K R
The molecular characterization of a cardiac determination gene has been an elusive goal for the past several years. Prior to cloning of the skeletal muscle determination factor MyoD, the presence of...
Zhu, H, Garcia, A V, Ross, R S, Evans, S M, Chien, K R
To study the transcriptional regulatory mechanisms which mediate cardiac-specific and inducible expression during myocardial cell hypertrophy, we have extensively characterized the rat cardiac myosin...
Zhu, H, Nguyen, V T, Brown, A B, Pourhosseini, A, Garcia, A V, Van Bilsen, M, ...
The AT-rich element MEF-2 plays an important role in the maintenance of the muscle-specific expression of a number of cardiac and skeletal muscle genes. In the MLC-2 gene, an AT-rich element (HF-1b)...
Navankasattusas, S, Zhu, H, Garcia, A V, Evans, S M, Chien, K R
Recent studies have identified a conserved 28-bp element (HF-1) within the rat cardiac MLC-2 gene which confers cardiac muscle-specific and inducible expression during myocardial cell hypertrophy....
Chien, K R, Sen, A, Reynolds, R, Chang, A, Kim, Y, Gunn, M D, ...
The present study utilized a cultured myocardial cell model to evaluate the relationship between the release of arachidonate from membrane phospholipids, and the progression of cell injury during ATP...
Sen, A, Miller, J C, Reynolds, R, Willerson, J T, Buja, L M, Chien, K R
Previous studies have suggested that phospholipid degradation is closely associated with the development of sarcolemmal membrane injury. This study was initiated to characterize the effects of...
Gruber, P J, Kubalak, S W, Pexieder, T, Sucov, H M, Evans, R M, Chien, K R
Retinoid-dependent pathways play a central role in regulating cardiac morphogenesis. Recently, we characterized gene-targeted RXR alpha -/- embryos, which display an atrial-like ventricular phenotype...
Elevated blood pressure and enhanced myocardial contractility in mice with severe IGF-1 deficiency.
Lembo, G, Rockman, H A, Hunter, J J, Steinmetz, H, Koch, W J, Ma, L, ...
To circumvent the embryonic lethality of a complete deficiency in insulin-like growth factor 1 (IGF-1), we generated mice homozygous for a site-specific insertional event that created a mutant IGF-1...
Navankasattusas, S, Sawadogo, M, Van Bilsen, M, Dang, C V, Chien, K R
Previous studies have documented that 250 bp of the rat cardiac ventricular myosin light-chain 2 (MLC-2v) promoter is sufficient to confer cardiac muscle-specific expression on a luciferase reporter...
Dyson, E, Sucov, H M, Kubalak, S W, Schmid-Schönbein, G W, DeLano, F A, Evans, R M, ...
We have recently characterized a cardiac model of ventricular chamber defects in retinoid X receptor alpha (RXR alpha) homozygous mutant (-/-) gene-targeted mice. These mice display generalized...
Retinoid-dependent pathways suppress myocardial cell hypertrophy.
Zhou, M D, Sucov, H M, Evans, R M, Chien, K R
Utilizing an in vitro model system of cardiac muscle cell hypertrophy, we have identified a retinoic acid (RA)-mediated pathway that suppresses the acquisition of specific features of the...
Expression cloning of cardiotrophin 1, a cytokine that induces cardiac myocyte hypertrophy.
Pennica, D, King, K L, Shaw, K J, Luis, E, Rullamas, J, Luoh, S M, ...
Heart failure continues to be a leading cause of mortality worldwide. A hallmark of this disease is dilated cardiac hypertrophy, which is accompanied by a reactivation of genes expressed in fetal...
Molecular and physiological alterations in murine ventricular dysfunction.
Rockman, H A, Ono, S, Ross, R S, Jones, L R, Karimi, M, Bhargava, V, ...
The present study reports the development and characterization of a murine model of right ventricular dysfunction following graded constriction in the pulmonary artery via microsurgical approaches....
O'Brien, T X, Lee, K J, Chien, K R
To study the process of ventricular specification during cardiogenesis, we examined the in situ expression of cardiac ventricular myosin light chain 2 (MLC-2v) mRNA during murine embryogenesis. As...
Shubeita, H E, Martinson, E A, Van Bilsen, M, Chien, K R, Brown, J H
A cultured myocardial cell model was used to examine the role of protein kinase C-dependent pathways in the transcriptional activation of two cardiac muscle genes [myosin light chain 2 (MLC-2) and...
Rockman, H A, Ross, R S, Harris, A N, Knowlton, K U, Steinhelper, M E, Field, L J, ...
To study the mechanisms that activate expression of the atrial natriuretic factor (ANF) gene during pressure-induced hypertrophy, we have developed and characterized an in vivo murine model of...
Knowlton, K U, Rockman, H A, Itani, M, Vovan, A, Seidman, C E, Chien, K R
To determine whether similar or divergent pathways mediate atrial natriuretic factor (ANF) induction in neonatal and hypertrophied adult ventricular myocardium, and to assess whether studies using an...
Transient assays in cultured ventricular muscle cells and studies in transgenic mice have identified two adjacent regulatory elements (HF-1a and HF-1b/MEF-2) as required to maintain ventricular...
Feldman, S T, Gjerset, R, Gately, D, Chien, K R, Feramisco, J R
Infection with the Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial cells. Expression of large T antigen occurred...
Duerr, R L, Huang, S, Miraliakbar, H R, Clark, R, Chien, K R, Ross, J
To determine whether additional hypertrophy would be beneficial or maladaptive in cardiac failure, the effects of insulin-like growth factor (IGF-1) were investigated in rats with left ventricular...
Thorburn, J, Carlson, M, Mansour, S J, Chien, K R, Ahn, N G, Thorburn, A
Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated...
Sills, M N, Xu, Y C, Baracchini, E, Goodman, R H, Cooperman, S S, Mandel, G, ...
This study examined the diversity of Na+ channel gene expression in intact cardiac tissue and purified myocardial cells. The screening of neonatal rat myocardial cell cDNA libraries with a conserved...
Lee, K J, Hickey, R, Zhu, H, Chien, K R
The cardiac myosin light-chain 2v (MLC-2v) gene has served as a model system to identify the pathways which restrict the expression of cardiac muscle genes to particular chambers of the heart during...
Evans, S M, Tai, L J, Tan, V P, Newton, C B, Chien, K R
The molecular characterization of a cardiac determination gene has been an elusive goal for the past several years. Prior to cloning of the skeletal muscle determination factor MyoD, the presence of...
Navankasattusas, S, Sawadogo, M, Van Bilsen, M, Dang, C V, Chien, K R
Previous studies have documented that 250 bp of the rat cardiac ventricular myosin light-chain 2 (MLC-2v) promoter is sufficient to confer cardiac muscle-specific expression on a luciferase reporter...
Zhu, H, Garcia, A V, Ross, R S, Evans, S M, Chien, K R
To study the transcriptional regulatory mechanisms which mediate cardiac-specific and inducible expression during myocardial cell hypertrophy, we have extensively characterized the rat cardiac myosin...
Zhu, H, Nguyen, V T, Brown, A B, Pourhosseini, A, Garcia, A V, Van Bilsen, M, ...
The AT-rich element MEF-2 plays an important role in the maintenance of the muscle-specific expression of a number of cardiac and skeletal muscle genes. In the MLC-2 gene, an AT-rich element (HF-1b)...
Navankasattusas, S, Zhu, H, Garcia, A V, Evans, S M, Chien, K R
Recent studies have identified a conserved 28-bp element (HF-1) within the rat cardiac MLC-2 gene which confers cardiac muscle-specific and inducible expression during myocardial cell hypertrophy....
Chien, K R, Sen, A, Reynolds, R, Chang, A, Kim, Y, Gunn, M D, ...
The present study utilized a cultured myocardial cell model to evaluate the relationship between the release of arachidonate from membrane phospholipids, and the progression of cell injury during ATP...
Sen, A, Miller, J C, Reynolds, R, Willerson, J T, Buja, L M, Chien, K R
Previous studies have suggested that phospholipid degradation is closely associated with the development of sarcolemmal membrane injury. This study was initiated to characterize the effects of...
Gruber, P J, Kubalak, S W, Pexieder, T, Sucov, H M, Evans, R M, Chien, K R
Retinoid-dependent pathways play a central role in regulating cardiac morphogenesis. Recently, we characterized gene-targeted RXR alpha -/- embryos, which display an atrial-like ventricular phenotype...
Elevated blood pressure and enhanced myocardial contractility in mice with severe IGF-1 deficiency.
Lembo, G, Rockman, H A, Hunter, J J, Steinmetz, H, Koch, W J, Ma, L, ...
To circumvent the embryonic lethality of a complete deficiency in insulin-like growth factor 1 (IGF-1), we generated mice homozygous for a site-specific insertional event that created a mutant IGF-1...
Jones, R. L., Miller, J. C., Hagler, H. K., Chien, K. R., Willerson, J. T., Buja, L. M.
The development of irreversible myocardial ischemic injury is associated with progressive degradation of membrane phospholipids, accumulation of arachidonate and other free fatty acids, and...
Burton, K. P., Buja, L. M., Sen, A., Willerson, J. T., Chien, K. R.
Alterations in triacylglycerol and phospholipid metabolism are known to occur during the evolution of myocardial ischemic injury. The purpose of this study was to explore potential relationships...
Myocardial ischemia: the pathogenesis of irreversible cell injury in ischemia.
Farber, J. L., Chien, K. R., Mittnacht, S.
Cells made ischemic rapidly manifest many distinct structural and functional alterations as a consequence of the depletion of their energy stores. In attempting to determine which of these are...
Chien, K. R., Bellary, A., Nicar, M., Mukherjee, A., Buja, L. M.
Previous studies have demonstrated that cardiac myocytes in the border zone of acute myocardial infarction become markedly overloaded with neutral lipid during the transition from reversible to...
Farber, J. L., Martin, J. T., Chien, K. R.
Ischemic rat liver tissue has been shown previously to exhibit a markedly accelerated rate of phospholipid degradation, producing a loss of almost one half the total cellular phospholipid with 3...
Chien, K. R., Peau, R. G., Farber, J. L.
Ligation of the left coronary artery of an adult rat heart results in the reproducible ischemic cell death of the entire free wall of the left ventricular myocardium. The time course of the...