K. Ramotar

Publication List Details

Number

24

Co-Authors

Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunit.

Tyrrell, G J, Ramotar, K, Toye, B, Boyd, B, Lingwood, C A, Brunton, J L

Verotoxin 1 (VT-1) and Shiga-like toxin II (SLT-II) bind to the glycosphingolipid (GSL), globotriaosylceramide (Gb3), whereas pig edema disease toxin (VTE) binds to globotetraosylceramide (Gb4) and...

Inhibition of PCR in Genital and Urine Specimens Submitted for Chlamydia trachomatis Testing

Toye, B., Woods, W., Bobrowska, M., Ramotar, K.

We determined the frequency of PCR inhibition in genital and urine specimens submitted for Chlamydia trachomatis testing using the internal control DNA provided with the COBAS AMPLICOR C. trachomatis...

Molecular epidemiology of Xanthomonas maltophilia colonization and infection in the hospital environment.

Laing, F P, Ramotar, K, Read, R R, Alfieri, N, Kureishi, A, Henderson, E A, ...

Between April 1992 and December 1993, 80 Xanthomonas maltophilia isolates were collected from 63 patients in three acute-care hospitals in Calgary, Alberta, Canada. On the basis of Centers for...

Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR.

Ramotar, K, Waldhart, B, Church, D, Szumski, R, Louie, T J

A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were...

Impact of free verotoxin testing on epidemiology of diarrhea caused by verotoxin-producing Escherichia coli.

Ramotar, K, Henderson, E, Szumski, R, Louie, T J

During a 10-week period in the summer of 1990, an epidemiologic investigation of the prevalence of verotoxin (VT)-producing Escherichia coli infection was conducted in Calgary, Alberta, Canada....

Clinical and epidemiological significance of enterococci intrinsically resistant to vancomycin (possessing the vanC genotype)

Toye, B, Shymanski, J, Bobrowska, M, Woods, W, Ramotar, K

Constitutive low-level vancomycin resistance is found intrinsically in certain enterococcal species and is encoded by vanC ligase genes. These intrinsically vancomycin-resistant enterococci (VRE)...

Modification of the glycolipid-binding specificity of vero cytotoxin by polymyxin B and other cyclic amphipathic peptides.

Head, S, Ramotar, K, Lingwood, C

Polymyxin B, an amphipathic cyclic decapeptide produced by Bacillus polymyxa, is routinely used in the extraction of the components from the periplasmic space of gram-negative bacteria. Vero...

Risk factors for acquiring ampicillin-resistant enterococci and clinical outcomes at a Canadian tertiary-care hospital.

McCarthy, A E, Victor, G, Ramotar, K, Toye, B

The number of ampicillin-resistant enterococci (ARE) was noted to be increased at our teaching hospital. To determine the risk factors for acquiring this organism and to compare clinical outcomes,...

Prevalence and Mechanisms of Erythromycin Resistance in Group A and Group B Streptococcus: Implications for Reporting Susceptibility Results

Desjardins, M., Delgaty, K. L., Ramotar, K., Seetaram, C., Toye, B.

Increased rates of erythromycin resistance among group B Streptococcus (GBS) and group A Streptococcus (GAS) have been reported. Cross-resistance to clindamycin may be present, depending on the...

Characterization of Shiga-like toxin I B subunit purified from overproducing clones of the SLT-I B cistron.

Ramotar, K, Boyd, B, Tyrrell, G, Gariepy, J, Lingwood, C, Brunton, J

The cistron encoding the B subunit of Escherichia coli Shiga-like toxin I (SLT-I) was cloned under control of the tac promoter in the expression vector pKK223-3 and the SLT-I B subunit was expressed...

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

Desjardins, M., Guibord, Christiane, Lalonde, B., Toye, B., Ramotar, K.

Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by PCR can be performed directly from nasal specimens with the IDI-MRSA assay. To improve the efficiency of screening, we...

Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunit.

Tyrrell, G J, Ramotar, K, Toye, B, Boyd, B, Lingwood, C A, Brunton, J L

Verotoxin 1 (VT-1) and Shiga-like toxin II (SLT-II) bind to the glycosphingolipid (GSL), globotriaosylceramide (Gb3), whereas pig edema disease toxin (VTE) binds to globotetraosylceramide (Gb4) and...

Inhibition of PCR in Genital and Urine Specimens Submitted for Chlamydia trachomatis Testing

Toye, B., Woods, W., Bobrowska, M., Ramotar, K.

We determined the frequency of PCR inhibition in genital and urine specimens submitted for Chlamydia trachomatis testing using the internal control DNA provided with the COBAS AMPLICOR C. trachomatis...

Molecular epidemiology of Xanthomonas maltophilia colonization and infection in the hospital environment.

Laing, F P, Ramotar, K, Read, R R, Alfieri, N, Kureishi, A, Henderson, E A, ...

Between April 1992 and December 1993, 80 Xanthomonas maltophilia isolates were collected from 63 patients in three acute-care hospitals in Calgary, Alberta, Canada. On the basis of Centers for...

Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR.

Ramotar, K, Waldhart, B, Church, D, Szumski, R, Louie, T J

A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were...

Impact of free verotoxin testing on epidemiology of diarrhea caused by verotoxin-producing Escherichia coli.

Ramotar, K, Henderson, E, Szumski, R, Louie, T J

During a 10-week period in the summer of 1990, an epidemiologic investigation of the prevalence of verotoxin (VT)-producing Escherichia coli infection was conducted in Calgary, Alberta, Canada....

Clinical and epidemiological significance of enterococci intrinsically resistant to vancomycin (possessing the vanC genotype)

Toye, B, Shymanski, J, Bobrowska, M, Woods, W, Ramotar, K

Constitutive low-level vancomycin resistance is found intrinsically in certain enterococcal species and is encoded by vanC ligase genes. These intrinsically vancomycin-resistant enterococci (VRE)...

Modification of the glycolipid-binding specificity of vero cytotoxin by polymyxin B and other cyclic amphipathic peptides.

Head, S, Ramotar, K, Lingwood, C

Polymyxin B, an amphipathic cyclic decapeptide produced by Bacillus polymyxa, is routinely used in the extraction of the components from the periplasmic space of gram-negative bacteria. Vero...

Risk factors for acquiring ampicillin-resistant enterococci and clinical outcomes at a Canadian tertiary-care hospital.

McCarthy, A E, Victor, G, Ramotar, K, Toye, B

The number of ampicillin-resistant enterococci (ARE) was noted to be increased at our teaching hospital. To determine the risk factors for acquiring this organism and to compare clinical outcomes,...

Prevalence and Mechanisms of Erythromycin Resistance in Group A and Group B Streptococcus: Implications for Reporting Susceptibility Results

Desjardins, M., Delgaty, K. L., Ramotar, K., Seetaram, C., Toye, B.

Increased rates of erythromycin resistance among group B Streptococcus (GBS) and group A Streptococcus (GAS) have been reported. Cross-resistance to clindamycin may be present, depending on the...

Characterization of Shiga-like toxin I B subunit purified from overproducing clones of the SLT-I B cistron.

Ramotar, K, Boyd, B, Tyrrell, G, Gariepy, J, Lingwood, C, Brunton, J

The cistron encoding the B subunit of Escherichia coli Shiga-like toxin I (SLT-I) was cloned under control of the tac promoter in the expression vector pKK223-3 and the SLT-I B subunit was expressed...

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

Desjardins, M., Guibord, Christiane, Lalonde, B., Toye, B., Ramotar, K.

Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by PCR can be performed directly from nasal specimens with the IDI-MRSA assay. To improve the efficiency of screening, we...