K. Sakai

Publication List Details

Period

1981 - 9999

Number

157

Co-Authors

Results Obtained during Acoustic Emission Monitoring of Impact Damage Graphite/Epoxy Pressure Vessels, (9999)

Gardiner,D. S., Gorman,M. R., Rytting,T. H., Sakai,K.

Acoustic emission (AE) sensors were used to monitor subscale graphite/epoxy pressure vessels that were subjected to increasing levels of impact damage. Impact damage was inflicted after each vessel...

Cosmic Ray Tests of the Prototype TPC for the ILC Experiment (2009)

Ackermann, K., Arai, S., Arogancia, D. C., Bacala, A. M., Ball, M., Behnke, T., ...

A time projection chamber (TPC) is a strong candidate for the central tracker of the international linear collider (ILC) experiment and we have been conducting a series of cosmic ray experiments...

Age constraints on the origin and growth history of a deep-water coral mound in the northeast Atlantic drilled during Integrated Ocean Drilling Program Expedition 307 (2007)

Kano, A., Ferdelman, T.G., Williams, T., Henriet, J-P., Ishikawa, T., Kawagoe, N., ...

Sr isotope stratigraphy provides a new age model for the first complete section drilled through a deep-water coral mound. The 155-m-long section from Challenger Mound in the Porcupine Sea-bight,...

A GEM TPC End-Panel Pre-Prototype (2007)

Ishikawa, A., Aoza, A., Higashi, T., Sugiyama, A., Tsuji, H., Kato, Y., ...

A GEM TPC end panel pre-prototype was constructed for a large LC-TPC prototype to test its basic design philosophy and some of its engineering details. Its interim test results are presented.

A novel GJB2 mutation p.Asn54His in a patient with palmoplantar keratoderma, sensorineural hearing loss and knuckle pads (2007)

Akiyama, M., Sakai, K., Arita, K., Nomura, Y., Ito, K., Kodama, K., ...

Mutations in the GJB2 gene encoding connexin26 are the major cause of autosomal-recessive or -dominant nonsyndromic congenital sensorineural hearing loss (SNHL) (Kelsell et al., 1997; Kenneson et...

On-site underground background measurements for the KASKA reactor-neutrino experiment (2006)

Furuta, H., Sakuma, K., Aoki, M., Fukuda, Y., Funaki, Y., Hara, T., ...

On-site underground background measurements were performed for the planned reactor-neutrino oscillation experiment KASKA at Kashiwazaki-Kariwa nuclear power station in Niigata, Japan. A...

Letter of Intent for KASKA: High Accuracy Neutrino Oscillation Measurements with anti-nu_es from Kashiwazaki-Kariwa Nuclear Power Station (2006)

Aoki, M., Akiyama, K., Fukuda, Y., Fukui, A., Funaki, Y., Furuta, H., ...

One of the current most-demanded experiments in neutrino physics is to measure the last mixing angle theta_13. KASKA is an experiment to detect new type of reactor neutrino oscillation and to measure...

Reading hidden intentions in the human brain (2006)

Haynes, J-D., Sakai, K., Rees, G., Gilbert, S., Frith, C., Passingham, R.E.

When humans are engaged in goal-related processing, activity in prefrontal cortex is increased [1, 2]. However, it has remained unclear whether this prefrontal activity encodes a subject’s current...

Algebraic Curve for the SO(6) sector of AdS/CFT (2006)

Beisert,Niklas, Kazakov,V. A., Sakai,K.

We construct the general algebraic curve of degree four solving the classical sigma model on RxS5. Up to two loops it coincides with the algebraic curve for the dual sector of scalar operators in N=4...

Algebraic Curve for the SO(6) sector of AdS/CFT (2006)

Beisert, Niklas, Kazakov, V. A., Sakai, K.

We construct the general algebraic curve of degree four solving the classical sigma model on RxS5. Up to two loops it coincides with the algebraic curve for the dual sector of scalar operators in N=4...

Characterizing the pH-responsive behavior of thin films of diblock copolymer micelles at the silica/aqueous solution interface (2006)

Sakai, K, Smith, Emelyn G., Webber, Grant B., Baker, Murray, Wanless, Erica J., Butun, Vural, ...

The pH-responsive behavior of cationic diblock poly(2-(dimethylamino)ethyl methacrylate)-block-poly(2-(diethylamino)ethyl methacrylate) copolymer micelles adsorbed at the silica/aqueous solution...

A Novel ABCA12 Mutation 3270delT Causes Harlequin Ichthyosis (2006)

Akiyama, M., Sakai, K., Wolff, G., Hausser, J. R., McMillan, J. R., Sawamura, D., ...

The article presents a case of a baby girl who had been suffering from harlequin ichthyosis, a severe fatal congenital ichthyosis. Its clinical features include thick, plate-like scales with...

Characterizing the pH-responsive behavior of thin films of diblock copolymer micelles at the silica/aqueous solution interface (2006)

Sakai, K, Smith, Emelyn G., Webber, Grant B., Baker, Murray, Wanless, Erica J., Butun, Vural, ...

The pH-responsive behavior of cationic diblock poly(2-(dimethylamino)ethyl methacrylate)-block-poly(2-(diethylamino)ethyl methacrylate) copolymer micelles adsorbed at the silica/aqueous solution...

The Parent of Misfit-Layered Cobalt Oxides: [Sr2O2]qCoO2 (2005)

Yamauchi, H., Sakai, K., Nagai, T., Matsui, Y., Karppinen, M.

Misfit-layered (ML) cobalt oxides of the general formula of [MmA2Om+2]qCoO2 have been proven to be efficient thermoelectric materials as the structure is capable in accommodating the two seemingly...

Complete Spectrum of Long Operators in N=4 SYM at One Loop (2005)

Beisert, N., Kazakov, V. A., Sakai, K., Zarembo, K.

We construct the complete spectral curve for an arbitrary local operator, including fermions and covariant derivatives, of one-loop N=4 gauge theory in the thermodynamic limit. This curve perfectly...

The Algebraic Curve of Classical Superstrings on AdS_5xS^5 (2005)

Beisert, N., Kazakov, V. A., Sakai, K., Zarembo, K.

We investigate the monodromy of the Lax connection for classical IIB superstrings on AdS_5xS^5. For any solution of the equations of motion we derive a spectral curve of degree 4+4. The curve...

Using proton-transfer laser dyes for organic laser diodes (2005)

Sakai, K, Tsuzuki, T, Itoh, Y, Ichikawa, M, Taniguchi, Y

Copyright (2005) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The...

Some experimental studies on time-of-flight radiography using a pulsed neutron source (2005)

Kiyanagi, Y., Sakamoto, N., Iwasa, H., Kamiyama, T., Hiraga, F., Sato, S., ...

We are developing a fiber type two-dimentional (2-D) position-sensitive detector having a spatial resolution less than 0.5 mm in order to use it in the time-of-flight (TOF) measurements. The detector...

Some experimental studies on time-of-flight radiography using a pulsed neutron source (2005)

Kiyanagi, Y., Sakamoto, N., Iwasa, H., Kamiyama, T., Hiraga, F., Sato, S., ...

We are developing a fiber type two-dimentional (2-D) position-sensitive detector having a spatial resolution less than 0.5 mm in order to use it in the time-of-flight (TOF) measurements. The detector...

Some experimental studies on time-of-flight radiography using a pulsed neutron source (2005)

Kiyanagi, Y., Sakamoto, N., Iwasa, H., Kamiyama, T., Hiraga, F., Sato, S., ...

We are developing a fiber type two-dimentional (2-D) position-sensitive detector having a spatial resolution less than 0.5 mm in order to use it in the time-of-flight (TOF) measurements. The detector...

Exact Analysis of ESR Shift in the Spin-1/2 Heisenberg Antiferromagnetic Chain (2005)

Maeda, Y., Sakai, K., Oshikawa, M.

A systematic perturbation theory is developed for the ESR shift and is applied to the spin-1/2 Heisenberg chain. Using the Bethe ansatz technique, we exactly analyze the resonance shift in the first...

Posttranslational modifications of decidual IGFBP-1 by steroid hormones in vitro (2005)

Kabir-Salmani, M., Shimizu, Y., Sakai, K., Iwashita, M.

Insulin-like growth factor binding protein-1 (IGFBP-1) appears to regulate insulin-like growth factors (IGFs; IGF-I and IGF-II) biological activity within the local environment of human placenta by...

Posttranslational modifications of decidual IGFBP-1 by steroid hormones in vitro (2005)

Kabir-Salmani, M., Shimizu, Y., Sakai, K., Iwashita, M.

Insulin-like growth factor binding protein-1 (IGFBP-1) appears to regulate insulin-like growth factors (IGFs; IGF-I and IGF–II) biological activity within the local environment of human placenta by...

Algebraic Curve for the SO(6) sector of AdS/CFT (2004)

Beisert, N., Kazakov, V. A., Sakai, K.

We construct the general algebraic curve of degree four solving the classical sigma model on RxS5. Up to two loops it coincides with the algebraic curve for the dual sector of scalar operators in N=4...

The role of {alpha}5{beta}1-integrin in the IGF-I-induced migration of extravillous trophoblast cells during the process of implantation (2004)

Kabir-Salmani, M., Shiokawa, S., Akimoto, Y., Sakai, K., Iwashita, M.

The role of integrins in the processes of adhesion and migration makes them attractive potential participants in the complex events of embryo implantation and placentation. Recently, the role of the...

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation (2003)

Sakai,T., Li,S. H., Docheva,D., Grashoff,C., Sakai,K., Kostka,G., ...

Integrin-mediated cell-matrix interactions are essential for development, tissue homeostasis, and repair. Upon ligand binding, integrins are recruited into focal adhesions (FAs). Integrin-linked...

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation (2003)

Sakai, T., Li, S. H., Docheva, D., Grashoff, C., Sakai, K., Kostka, G., ...

Integrin-mediated cell-matrix interactions are essential for development, tissue homeostasis, and repair. Upon ligand binding, integrins are recruited into focal adhesions (FAs). Integrin-linked...

Measurement of parity-nonconserving rotation of neutron spin in the 0.734-eV p-wave resonance of $^{139}La$ (2001)

Haseyama, T., Asahi, K., Bowman, J. D., Delheij, P. P. J., Funahashi, H., Ishimoto, S., ...

The parity nonconserving spin rotation of neutrons in the 0.734-eV p-wave resonance of $^{139}La$ was measured with the neutron transmission method. Two optically polarized $^3He$ cells were used...

Synopsis of the family Callianassidae, with keys to subfamilies, genera and species, and the description of new taxa (Crustacea: Decapoda: Thalassinidea) (1999)

Sakai, K.

A synopsis of the family Callianassidae is presented. Defenitions are given of the subfamilies and genera. Keys to the sufamilies, genera, as well as seperate keys to the species occurring in certain...

Improvement and Restoration of Biomedical Images Blurred by Body Movement (1998)

Hori, J., Saitoh, Y., Kiryu, T., Okamoto, K., Sakai, K.

The restoration of biomedical images that have been blurred due to body movement are discussed. The observation system for these images is described using a mathematical operator and coordinate...

High-Pressure Phase Diagram of an Aluminium-Rich Al-Li Alloy at a Pressure of 5.4 GPa. (1998)

Matsumuro, A., Sakai, K., Senoo, M.

The high-pressure phase diagram of an aluminum-rich Al-Li alloy at a 5.4 GPa pressure was investigated; to determine the equilibrium state under high-pressure and high-temperature conditions, the...

Effect of Silver Coating on the Superconducting and Mechanical Properties of Solution-Spun Y1Ba2Cu3Ox Filament. (1998)

Goto, T., Sakai, K., Kawanaka, A.

This paper concerned mmc in some important way and contained information about, utilized, or discussed these subjects: basic topics:, composite materials, data, experimental, reinforcement data...

Free energies and critical exponents of the A_1^{(1)}, B_n^{(1)}, C_n^{(1)} and D_n^{(1)} face models (1997)

Batchelor, M. T., Fridkin, V., Kuniba, A., Sakai, K.

We obtain the free energies and critical exponents of models associated with elliptic solutions of the star-triangle relation and reflection equation. The models considered are related to the affine...

Continuum-wise expansive diffeomorphisms (1997)

K. Sakai

In this paper, we show that the $C^1$ interior of the set of all continuum-wise expansive diffeomorphisms of a closed manifold coincides with the $C^1$ interior of the set of all expansive...

The effect of sodium and ultrafiltration modelling on plasma volume changes and haemodynamic stability in intensive care patients receiving haemodialysis for acute renal failure: a prospective, stratified, randomized, cross-over study (1996)

Paganini, E. P., Sandy, D., Moreno, L., Kozlowski, L., Sakai, K.

Background Haemodynamic stability in intensive care unit (ICU) patient with acute renal failure (ARF) during intermittent dialytic support has been the focus for several variations to dialysis...

Two species of Upogebia from Tokushima, Japan, with a description of a new species, Upogebia trispinosa (Crustacea: Decapoda: Thalassinidea) (1991)

Sakai, K., Mukai, H.

Upogebia trispinosa spec. nov. is described from Tokushima, Japan. The species is rather closely related to U. narutensis.

Varying Methods of Sterilisation, and Their Effects on the Structure and Permeability of Dialysis Membranes (1987)

Takesawa, S., Ohmi, S., Konno, Y., Sekiguchi, M., Shitaokoshi, S., Takahashi, T., ...

This study elucidates changes in membrane structure and permeability due to the methods of sterilisation and the conditions under which they are carried out. Tubular dialysis membranes of regenerated...

PRESENT STATUS OF UVSOR (1986)

Watanabe, M, Kasuga, T, Yonehara, H, Uchida, A, Sakai, K, Matsudo, O, ...

In *Tsukuba 1984, Proceedings, Accelerator Science and Technology*, 15-17

Relative Biological Effectiveness of Tritiated Water on Cultured Mammalian Cells at Molecular and Cellular Level (1986)

Okada, S., Sakai, K., Nakamura, N.

Factors that affect RBE values have been investigated in cultured cells. It was shown that: (1) Different RBE values were obtained with the same tritiated water treated cells depending upon the...

Some Upogebiidae (Crustacea, Decapoda) in the collection of the Rijksmuseum van Natuurlijke Historie, Leiden (1984)

Sakai, K.

Unidentified specimens of the genus Upogebia in the collection of the Rijksmuseum van Natuurlijke Historie in Leiden were studied. A ♀ from Angola proved to be closely related to U. subspinosa; it...

STRUCTURAL MODEL OF FLUORINATED AMORPHOUS-SILICON (a-Si : F) (1981)

Matsumura, H., Sakai, K., Kawakyu, Y., Furukawa, S.

A structural model for fluorinated amorphous-silicon containing no hydrogen (a-Si : F) is presented based on the experimental results of transmission electron microscopic measurement, infrared...

STRUCTURAL MODEL OF FLUORINATED AMORPHOUS-SILICON (a-Si : F) (1981)

Matsumura, H., Sakai, K., Kawakyu, Y., Furukawa, S.

A structural model for fluorinated amorphous-silicon containing no hydrogen (a-Si : F) is presented based on the experimental results of transmission electron microscopic measurement, infrared...

STRUCTURAL MODEL OF FLUORINATED AMORPHOUS-SILICON (a-Si : F) (1981)

Matsumura, H., Sakai, K., Kawakyu, Y., Furukawa, S.

A structural model for fluorinated amorphous-silicon containing no hydrogen (a-Si : F) is presented based on the experimental results of transmission electron microscopic measurement, infrared...

Activity of primate inferotemporal neurons related to a sought target in pair-association task.

Naya, Y, Sakai, K, Miyashita, Y

Visual long-term memory in primates has been assessed by using the pair-association (PA) task, in which a subject retrieves and chooses the paired associate of a cue picture. Our previous studies on...

AlgR2 is an ATP/GTP-dependent protein kinase involved in alginate synthesis by Pseudomonas aeruginosa.

Roychoudhury, S, Sakai, K, Chakrabarty, A M

The exopolysaccharide alginate is a major virulence factor in the pathogenicity of Pseudomonas aeruginosa infecting the lungs of cystic fibrosis (CF) patients. Alginate synthesis by P. aeruginosa is...

Degradation of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans by the white rot fungus Phanerochaete sordida YK-624.

Takada, S, Nakamura, M, Matsueda, T, Kondo, R, Sakai, K

A method for the degradation of dioxins by white rot fungi was developed. Degradation of a mixture of 10 kinds of tetra- to octachlorodibenzo-p-dioxins (polychlorinated dibenzo-p-dioxins [PCDDs]) and...

Production and characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1.

Sakai, K, Oshima, K, Moriguchi, M

N-Acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1 was inducibly produced by D isomers of N-acetylglutamate, glutamate, aspartate, and asparagine. The enzyme has been purified to...

The alpha form of human tryptase is the predominant type present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosis.

Schwartz, L B, Sakai, K, Bradford, T R, Ren, S, Zweiman, B, Worobec, A S, ...

Tryptase, a protease produced by all mast cells, was evaluated as a clinical marker of systemic mastocytosis. Two sandwich immunoassays were evaluated, one which used the mAb G5 for capture, the...

Purification and characterization of thermostable beta-N-acetylhexosaminidase of Bacillus stearothermophilus CH-4 isolated from chitin-containing compost.

Sakai, K, Narihara, M, Kasama, Y, Wakayama, M, Moriguchi, M

Thermostable exochitinase was purified to homogeneity from the culture fluid of Bacillus stearothermophilus CH-4, which was isolated from agricultural compost containing shrimp and crabs. The enzyme...

Recombinational analysis of a natural noncytopathic human immunodeficiency virus type 1 (HIV-1) isolate: role of the vif gene in HIV-1 infection kinetics and cytopathicity.

Sakai, K, Ma, X Y, Gordienko, I, Volsky, D J

Two molecularly cloned coisolates of human immunodeficiency virus type 1 (HIV-1) have been found to exhibit different phenotypes of viral expression, either rapid and cytopathic (N1T-A virus) or...

Differences in cytopathogenicity and host cell range among infectious molecular clones of human immunodeficiency virus type 1 simultaneously isolated from an individual.

Sakai, K, Dewhurst, S, Ma, X Y, Volsky, D J

A cytopathic human immunodeficiency virus type 1 (HIV-1) isolate containing multiple virus genotypes was molecularly cloned, and the biological activity of six randomly selected clones was assessed...

Characterization of the env gene and long terminal repeat of molecularly cloned Friend mink cell focus-inducing virus DNA.

Adachi, A, Sakai, K, Kitamura, N, Nakanishi, S, Niwa, O, Matsuyama, M, ...

The highly oncogenic erythroleukemia-inducing Friend mink cell focus-inducing (MCF) virus was molecularly cloned in phage lambda gtWES.lambda B, and the DNA sequences of the env gene and the long...

Persistent productive infection of human glial cells by human immunodeficiency virus (HIV) and by infectious molecular clones of HIV.

Dewhurst, S, Sakai, K, Bresser, J, Stevenson, M, Evinger-Hodges, M J, Volsky, D J

The nature of the interaction between human immunodeficiency virus (HIV) and human cells of astrocytic origin was studied in vitro with cultured glial cells and intact HIV or infectious molecular...

Fv-1 determinants in xenotropic murine leukemia viruses studied with biological assay systems: Isolation of xenotropic virus with N-tropic Fv-1 activity in the cryptic form.

Sakai, K, Narita, H, Adachi, A, Tsuruta, S, Yorifuji, T, Ishimoto, A

By a biological assay system using phenotypically mixed ecotropic and xenotropic murine leukemia viruses, we investigated whether in the virions of a xenotropic virus there is N- or B-tropic Fv-1...

Involvement of distinct murine T-cell receptors in the autoimmune encephalitogenic response to nested epitopes of myelin basic protein.

Sakai, K, Sinha, A A, Mitchell, D J, Zamvil, S S, Rothbard, J B, McDevitt, H O, ...

The peptide p89-101 (Val-His-Phe-Phe-Lys-Asn-Ile-Val-Thr-Pro-Arg-Thr-Pro) of myelin basic protein is encephalitogenic in mice expressing H-2q and H-2s antigens. Six of 13 encephalitogen-specific...

Prevention of experimental encephalomyelitis with peptides that block interaction of T cells with major histocompatibility complex proteins.

Sakai, K, Zamvil, S S, Mitchell, D J, Hodgkinson, S, Rothbard, J B, Steinman, L

Two synthetic immunodominant and nonencephalitogenic peptides of myelin basic protein, N1-20 and AcN9-20, effectively compete with an encephalitogenic peptide, AcN1-11, in an in vitro T-cell response...

Synthesis of a novel cytokine and its gene (LD78) expressions in hematopoietic fresh tumor cells and cell lines.

Yamamura, Y, Hattori, T, Obaru, K, Sakai, K, Asou, N, Takatsuki, K, ...

The gene coding for the protein LD78 was isolated from stimulated human tonsillar lymphocytes by differential hybridization. The gene product consisted of 92 amino acids with characteristics of...

High-frequency cotransformation by copolymerization of plasmids in the fission yeast Schizosaccharomyces pombe.

Sakai, K, Sakaguchi, J, Yamamoto, M

We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on...

Biological and Chemical Detection of Trichothecene Mycotoxins of Fusarium Species

Ueno, Y., Sato, N., Ishii, K., Sakai, K., Tsunoda, H., Enomoto, M.

The procedure for biological and chemical detection of trichothecene-type mycotoxins and its application to the screening of Fusarium for toxic strains were described.

Differential amplification, assembly, and relocation of multiple DNA sequences in human neuroblastomas and neuroblastoma cell lines.

Shiloh, Y, Shipley, J, Brodeur, G M, Bruns, G, Korf, B, Donlon, T, ...

DNA amplification, manifested by homogeneously staining regions in chromosomes and by extrachromosomal, double minute bodies, is characteristic of many neuroblastoma cell lines. Sequences recruited...

A novel case of a sporadic desmoid tumour with mutation of the beta catenin gene.

Shitoh, K, Konishi, F, Iijima, T, Ohdaira, T, Sakai, K, Kanazawa, K, ...

A 42 year old man without familial adenomatous polyposis had recurrent desmoid tumours in the left subclavicular site. Histological examination showed a typical desmoid tumour. Molecular analysis was...

A novel heparin-dependent processing pathway for human tryptase. Autocatalysis followed by activation with dipeptidyl peptidase I.

Sakai, K, Ren, S, Schwartz, L B

Tryptase is the major protein constituent of human mast cells, where it is stored within the secretory granules as a fully active tetramer. Two tryptase genes (alpha and beta) are expressed by human...

Bleaching of Hardwood Kraft Pulp with Manganese Peroxidase from Phanerochaete sordida YK-624 without Addition of MnSO(inf4)

Harazono, K., Kondo, R., Sakai, K.

In vitro bleaching of an unbleached hardwood kraft pulp was performed with partially purified manganese peroxidase (MnP) from the fungus Phanerochaete sordida YK-624 without the addition of...

Comparison of Pressure Resistances of Spores of Six Bacillus Strains with Their Heat Resistances

Nakayama, A., Yano, Y., Kobayashi, S., Ishikawa, M., Sakai, K.

The pressure resistances of the spores of six Bacillus strains were examined at 5 to 10(deg)C and were compared with their heat resistances. The pressure treatments (at 981 MPa for 40 min and at 588...

The relation between carcinoma of the gallbladder and an anomalous connection between the choledochus and the pancreatic duct.

Nagata, E, Sakai, K, Kinoshita, H, Kobayashi, Y

An anomalous connection between the choledochus and the pancreatic duct may be associated etiologically or pathogenetically with congenital biliary dilation and carcinoma of the dilated bile duct....

Activity of primate inferotemporal neurons related to a sought target in pair-association task.

Naya, Y, Sakai, K, Miyashita, Y

Visual long-term memory in primates has been assessed by using the pair-association (PA) task, in which a subject retrieves and chooses the paired associate of a cue picture. Our previous studies on...

AlgR2 is an ATP/GTP-dependent protein kinase involved in alginate synthesis by Pseudomonas aeruginosa.

Roychoudhury, S, Sakai, K, Chakrabarty, A M

The exopolysaccharide alginate is a major virulence factor in the pathogenicity of Pseudomonas aeruginosa infecting the lungs of cystic fibrosis (CF) patients. Alginate synthesis by P. aeruginosa is...

Degradation of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans by the white rot fungus Phanerochaete sordida YK-624.

Takada, S, Nakamura, M, Matsueda, T, Kondo, R, Sakai, K

A method for the degradation of dioxins by white rot fungi was developed. Degradation of a mixture of 10 kinds of tetra- to octachlorodibenzo-p-dioxins (polychlorinated dibenzo-p-dioxins [PCDDs]) and...

Production and characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1.

Sakai, K, Oshima, K, Moriguchi, M

N-Acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1 was inducibly produced by D isomers of N-acetylglutamate, glutamate, aspartate, and asparagine. The enzyme has been purified to...

The alpha form of human tryptase is the predominant type present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosis.

Schwartz, L B, Sakai, K, Bradford, T R, Ren, S, Zweiman, B, Worobec, A S, ...

Tryptase, a protease produced by all mast cells, was evaluated as a clinical marker of systemic mastocytosis. Two sandwich immunoassays were evaluated, one which used the mAb G5 for capture, the...

Purification and characterization of thermostable beta-N-acetylhexosaminidase of Bacillus stearothermophilus CH-4 isolated from chitin-containing compost.

Sakai, K, Narihara, M, Kasama, Y, Wakayama, M, Moriguchi, M

Thermostable exochitinase was purified to homogeneity from the culture fluid of Bacillus stearothermophilus CH-4, which was isolated from agricultural compost containing shrimp and crabs. The enzyme...

Recombinational analysis of a natural noncytopathic human immunodeficiency virus type 1 (HIV-1) isolate: role of the vif gene in HIV-1 infection kinetics and cytopathicity.

Sakai, K, Ma, X Y, Gordienko, I, Volsky, D J

Two molecularly cloned coisolates of human immunodeficiency virus type 1 (HIV-1) have been found to exhibit different phenotypes of viral expression, either rapid and cytopathic (N1T-A virus) or...

Differences in cytopathogenicity and host cell range among infectious molecular clones of human immunodeficiency virus type 1 simultaneously isolated from an individual.

Sakai, K, Dewhurst, S, Ma, X Y, Volsky, D J

A cytopathic human immunodeficiency virus type 1 (HIV-1) isolate containing multiple virus genotypes was molecularly cloned, and the biological activity of six randomly selected clones was assessed...

Characterization of the env gene and long terminal repeat of molecularly cloned Friend mink cell focus-inducing virus DNA.

Adachi, A, Sakai, K, Kitamura, N, Nakanishi, S, Niwa, O, Matsuyama, M, ...

The highly oncogenic erythroleukemia-inducing Friend mink cell focus-inducing (MCF) virus was molecularly cloned in phage lambda gtWES.lambda B, and the DNA sequences of the env gene and the long...

Persistent productive infection of human glial cells by human immunodeficiency virus (HIV) and by infectious molecular clones of HIV.

Dewhurst, S, Sakai, K, Bresser, J, Stevenson, M, Evinger-Hodges, M J, Volsky, D J

The nature of the interaction between human immunodeficiency virus (HIV) and human cells of astrocytic origin was studied in vitro with cultured glial cells and intact HIV or infectious molecular...

Fv-1 determinants in xenotropic murine leukemia viruses studied with biological assay systems: Isolation of xenotropic virus with N-tropic Fv-1 activity in the cryptic form.

Sakai, K, Narita, H, Adachi, A, Tsuruta, S, Yorifuji, T, Ishimoto, A

By a biological assay system using phenotypically mixed ecotropic and xenotropic murine leukemia viruses, we investigated whether in the virions of a xenotropic virus there is N- or B-tropic Fv-1...

Involvement of distinct murine T-cell receptors in the autoimmune encephalitogenic response to nested epitopes of myelin basic protein.

Sakai, K, Sinha, A A, Mitchell, D J, Zamvil, S S, Rothbard, J B, McDevitt, H O, ...

The peptide p89-101 (Val-His-Phe-Phe-Lys-Asn-Ile-Val-Thr-Pro-Arg-Thr-Pro) of myelin basic protein is encephalitogenic in mice expressing H-2q and H-2s antigens. Six of 13 encephalitogen-specific...

Prevention of experimental encephalomyelitis with peptides that block interaction of T cells with major histocompatibility complex proteins.

Sakai, K, Zamvil, S S, Mitchell, D J, Hodgkinson, S, Rothbard, J B, Steinman, L

Two synthetic immunodominant and nonencephalitogenic peptides of myelin basic protein, N1-20 and AcN9-20, effectively compete with an encephalitogenic peptide, AcN1-11, in an in vitro T-cell response...

Synthesis of a novel cytokine and its gene (LD78) expressions in hematopoietic fresh tumor cells and cell lines.

Yamamura, Y, Hattori, T, Obaru, K, Sakai, K, Asou, N, Takatsuki, K, ...

The gene coding for the protein LD78 was isolated from stimulated human tonsillar lymphocytes by differential hybridization. The gene product consisted of 92 amino acids with characteristics of...

High-frequency cotransformation by copolymerization of plasmids in the fission yeast Schizosaccharomyces pombe.

Sakai, K, Sakaguchi, J, Yamamoto, M

We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on...

Biological and Chemical Detection of Trichothecene Mycotoxins of Fusarium Species

Ueno, Y., Sato, N., Ishii, K., Sakai, K., Tsunoda, H., Enomoto, M.

The procedure for biological and chemical detection of trichothecene-type mycotoxins and its application to the screening of Fusarium for toxic strains were described.

Differential amplification, assembly, and relocation of multiple DNA sequences in human neuroblastomas and neuroblastoma cell lines.

Shiloh, Y, Shipley, J, Brodeur, G M, Bruns, G, Korf, B, Donlon, T, ...

DNA amplification, manifested by homogeneously staining regions in chromosomes and by extrachromosomal, double minute bodies, is characteristic of many neuroblastoma cell lines. Sequences recruited...

A novel case of a sporadic desmoid tumour with mutation of the beta catenin gene.

Shitoh, K, Konishi, F, Iijima, T, Ohdaira, T, Sakai, K, Kanazawa, K, ...

A 42 year old man without familial adenomatous polyposis had recurrent desmoid tumours in the left subclavicular site. Histological examination showed a typical desmoid tumour. Molecular analysis was...

A novel heparin-dependent processing pathway for human tryptase. Autocatalysis followed by activation with dipeptidyl peptidase I.

Sakai, K, Ren, S, Schwartz, L B

Tryptase is the major protein constituent of human mast cells, where it is stored within the secretory granules as a fully active tetramer. Two tryptase genes (alpha and beta) are expressed by human...

The relation between carcinoma of the gallbladder and an anomalous connection between the choledochus and the pancreatic duct.

Nagata, E, Sakai, K, Kinoshita, H, Kobayashi, Y

An anomalous connection between the choledochus and the pancreatic duct may be associated etiologically or pathogenetically with congenital biliary dilation and carcinoma of the dilated bile duct....

Bleaching of Hardwood Kraft Pulp with Manganese Peroxidase from Phanerochaete sordida YK-624 without Addition of MnSO(inf4)

Harazono, K., Kondo, R., Sakai, K.

In vitro bleaching of an unbleached hardwood kraft pulp was performed with partially purified manganese peroxidase (MnP) from the fungus Phanerochaete sordida YK-624 without the addition of...

Comparison of Pressure Resistances of Spores of Six Bacillus Strains with Their Heat Resistances

Nakayama, A., Yano, Y., Kobayashi, S., Ishikawa, M., Sakai, K.

The pressure resistances of the spores of six Bacillus strains were examined at 5 to 10(deg)C and were compared with their heat resistances. The pressure treatments (at 981 MPa for 40 min and at 588...

Cyclic adenosine 3',5'-monophosphate metabolism in activated T-cell clones.

Oksenberg, D, Oksenberg, J R, Sakai, K, Peroutka, S J, Steinman, L

The role of cAMP in lymphocyte proliferation was investigated in the response of a monoclonal T-cell population to a specific antigen and compared to the response to interleukin-2 (IL-2) and...

Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs

Okazaki, Y, Furuno, M, Kasukawa, T, Adachi, J, Bono, H, Kondo, S, ...

Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the...

Inactivation of C1 in rheumatoid synovial fluid, purified C1 and C1 esterase, by gold compounds*

Schultz, D. R., Volanakis, J. E., Arnold, Patricia I., Gottlieb, N. L., Sakai, K., Stroud, R. M.

The effects of gold compounds on the functional activities of rheumatoid synovial fluid and normal serum Cl, C4, and C2 were investigated in vitro. Commercial and purified gold sodium thiomalate in...

Effect of different sensitizing doses of antigen in a murine model of atopic asthma

Sakai, K, Yokoyama, A, Kohno, N, Hiwada, K

The dose of antigen is assumed to be one of the important factors in the polarized development of helper T cell subsets, i.e. Th1 or Th2 cells. We investigated the effect of the sensitizing antigen...

Role of 5-hydroxytryptamine in the vasoconstrictor action of compound 48/80 in the rat femoral vasculature.

Sakai, K

1. The effect of compound 48/80 on the rat femoral vasculature was examined by means of a cross-circulation technique. 2. Intra-arterial injection of increasing doses (1.5 to 15 microgram) of...

Demonstration in Tupaia papillary muscle preparations of alpha-adrenoceptors mediating positive inotropic effects: comparison with guinea-pigs.

Koga, T., Shiraki, Y., Sakai, K.

1. Positive inotropic responses to alpha- and beta-adrenoceptor agonists of isolated papillary muscles from the tree shrew (Tupaia), were compared with those from guinea-pigs. 2. In Tupaia, the...

Nicorandil attenuates myocardial acidosis during coronary occlusion in dogs.

Abiko, Y., Nishimura, T., Sakai, K.

In dogs anaesthetized with pentobarbitone, the left anterior descending coronary artery (LAD) was partially occluded. Before and 30 min after partial occlusion, the myocardial pH was 7.52-7.54 and...

The actions of 5-hydroxytryptamine and histamine on the isolated ileum of the tree shrew (Tupaia glis).

Sakai, K., Shiraki, Y., Tatsumi, T., Tsuji, K.

1. Contractions to 5-hydroxytryptamine (5-HT) and histamine of longitudinal muscle from the isolated ileum of the tree shrew (Tupaia), guinea-pig and rat were investigated by constructing...

A pharmacological analysis of the contractile action of histamine upon the ileal region of the isolated, blood-perfused small intestine of the rat.

Sakai, K.

1. The mode of action of histamine was investigated on the rat isolated small intestine perfused at a fixed flow rate through the superior mesenteric artery with blood from the carotid artery of a...

Coronary vasoconstriction by locally administered acetylcholine, carbachol and bethanechol in isolated, donor-perfused, rat hearts.

Sakai, K.

1 Experiments were carried out on rat isolated heart preparations in which the coronary vasculature was perfused through the aorta at a constant flow rate with arterial blood from donor animals....

Conformation and stability of thiol-modified bovine beta-lactoglobulin.

Sakai, K., Sakurai, K., Sakai, M., Hoshino, M., Goto, Y.

Bovine beta-lactoglobulin A assumes a dimeric native conformation at neutral pH, while the conformation at pH 2 is monomeric but still native. Beta-lactoglobulin A has a free thiol at Cys121, which...

Neuropathological studies of the spinal cord in early stage HTLV-I-associated myelopathy (HAM).

Yoshioka, A, Hirose, G, Ueda, Y, Nishimura, Y, Sakai, K

Necropsy findings for a patient with HTLV-I-associated myelopathy (HAM) of 9 months clinical duration are reported. Loss of myelin sheaths and axons together with perivascular lymphocytic...

Naming difficulties in alexia with agraphia for kanji after a left posterior inferior temporal lesion.

Sakurai, Y, Sakai, K, Sakuta, M, Iwata, M

The case is described of a patient with alexia and agraphia for kanji, and severe anomia after a subcortical haemorrhage in the left posterior inferior temporal area. Magnetic resonance imaging at...

Urinary kallikrein excretion in children of parents with essential hypertension.

Uchiyama, M, Otsuka, T, Sakai, K

Twelve hour urinary kallikrein excretion was measured in 18 healthy children of parents with essential hypertension and in 47 healthy children of parents without this disorder. No statistically...

Phosphorothioate oligonucleotides, suramin and heparin inhibit DNA-dependent protein kinase activity

Hosoi, Y, Matsumoto, Y, Tomita, M, Enomoto, A, Morita, A, Sakai, K, ...

Phosphorothioate oligonucleotides and suramin bind to heparin binding proteins including DNA polymerases, and inhibit their functions. In the present study, we report inhibition of DNA-dependent...