Sequence, regulation, and functions of fis in Salmonella typhimurium.
Osuna, R, Lienau, D, Hughes, K T, Johnson, R C
The fis operon from Salmonella typhimurium has been cloned and sequenced, and the properties of Fis-deficient and Fis-constitutive strains were examined. The overall fis operon organization in S....
Karlinsey, J E, Pease, A J, Winkler, M E, Bailey, J L, Hughes, K T
The flagellum of Salmonella typhimurium is assembled in stages, and the negative regulatory protein, FlgM, is able to sense the completion of an intermediate stage of assembly, the basal body-hook...
Hughes, K T, Dessen, A, Gray, J P, Grubmeyer, C
The Salmonella typhimurium nadC gene and its product, quinolinic acid phosphoribosyltransferase (QAPRTase), were characterized at the molecular and biochemical levels. Fusions of Mud-lac elements...
The flgM gene product has been shown to be a negative regulator of flagellin transcription in Salmonella typhimurium (K. L. Gillen and K. T. Hughes, J. Bacteriol. 173:2301-2310, 6453-6459, 1991; K....
The complex regulation of flagellin gene expression in Salmonella typhimurium was characterized in vivo by using lac transcriptional fusions to the two flagellin structural genes (fliC [H1] and fljB...
The expression of flagellin in Salmonella typhimurium is coupled to the assembly of complete flagella. Mutations which disrupt this coupling define a gene, flgM, which represses the expression of the...
DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium.
Park, U E, Olivera, B M, Hughes, K T, Roth, J R, Hillyard, D R
Bacterial DNA ligases use NAD as an energy source. In this study we addressed two questions about these enzymes. First, what is the physiological consequence of completely removing the NAD-dependent...
Hughes, K T, Simons, R W, Nunn, W D
Localized mutagenesis of the fadR region of the Escherichia coli chromosome resulted in the isolation of two classes of fadR regulatory mutants. The first class was constitutive for the fatty acid...
Structural gene for NAD synthetase in Salmonella typhimurium.
Hughes, K T, Olivera, B M, Roth, J R
We have identified the structural gene for NAD synthetase, which catalyzes the final metabolic step in NAD biosynthesis. This gene, designated nadE, is located between gdh and nit at 27 min on the...
Rec dependence of mu transposition from P22-transduced fragments.
Hughes, K T, Olivera, B M, Roth, J R
Derivatives of bacteriophage Mu carrying a lac operon and a selectable drug resistance element (Mu d phages) are frequently used tools of bacterial genetics. Mu d prophages used in this way can be...
Subunit-specific phenotypes of Salmonella typhimurium HU mutants.
Hillyard, D R, Edlund, M, Hughes, K T, Marsh, M, Higgins, N P
Salmonella hupA and hupB mutants were studied to determine the reasons for the high degree of conservation in HU structure in bacteria. We found one HU-1-specific effect; the F'128 plasmid was...
Conditionally transposition-defective derivative of Mu d1(Amp Lac).
A Mu d1 derivative is described which is useful for genetic manipulation of Mu-lac fusion insertions. A double mutant of the specialized transducing phage Mu d1(Amp Lac c62ts) was isolated which is...
6-Aminonicotinamide-resistant mutants of Salmonella typhimurium.
Hughes, K T, Cookson, B T, Ladika, D, Olivera, B M, Roth, J R
Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. (i) pncX mutants have Tn10 insertion...
Simons, R W, Hughes, K T, Nunn, W D
Strains stably merodiploid in the 25-min region of the chromosome of Escherichia coli were constructed and used in dominance tests between various wild-type and mutant alleles of the fadR gene....
Hughes, K T, Gaines, P C, Karlinsey, J E, Vinayak, R, Simon, M I
The Hin recombinase of Salmonella catalyzes a site-specific recombination event which leads to flagellar phase variation. Starting with a fully symmetrical recombination site, hixC, a set of 40...
An indispensable gene for NAD biosynthesis in Salmonella typhimurium.
Hughes, K T, Ladika, D, Roth, J R, Olivera, B M
We have located the nadD locus between lip and leuS at 14 min on the Salmonella typhimurium chromosome, and we have shown it to be the structural gene for nicotinic acid mononucleotide...
A genetic complementation system is described in which the complementing components are close together in a single linear DNA fragment; the complementation situation is temporary. This system is...
A Genetic Characterization of the Nadc Gene of Salmonella Typhimurium
Hughes, K. T., Roth, J. R., Olivera, B. M.
The nadC gene of Salmonella encodes the pyridine biosynthetic enzyme PRPP-quinolinate phosphoribosyltransferase. Using a combination of genetic techniques, a deletion map for the Salmonella nadC gene...
The Hin recombinase catalyzes a site-specific recombination reaction that results in the reversible inversion of a 1-kbp segment of the Salmonella chromosome. The DNA inversion reaction catalyzed by...
Sequence, regulation, and functions of fis in Salmonella typhimurium.
Osuna, R, Lienau, D, Hughes, K T, Johnson, R C
The fis operon from Salmonella typhimurium has been cloned and sequenced, and the properties of Fis-deficient and Fis-constitutive strains were examined. The overall fis operon organization in S....
Karlinsey, J E, Pease, A J, Winkler, M E, Bailey, J L, Hughes, K T
The flagellum of Salmonella typhimurium is assembled in stages, and the negative regulatory protein, FlgM, is able to sense the completion of an intermediate stage of assembly, the basal body-hook...
Hughes, K T, Dessen, A, Gray, J P, Grubmeyer, C
The Salmonella typhimurium nadC gene and its product, quinolinic acid phosphoribosyltransferase (QAPRTase), were characterized at the molecular and biochemical levels. Fusions of Mud-lac elements...
The flgM gene product has been shown to be a negative regulator of flagellin transcription in Salmonella typhimurium (K. L. Gillen and K. T. Hughes, J. Bacteriol. 173:2301-2310, 6453-6459, 1991; K....
The complex regulation of flagellin gene expression in Salmonella typhimurium was characterized in vivo by using lac transcriptional fusions to the two flagellin structural genes (fliC [H1] and fljB...
The expression of flagellin in Salmonella typhimurium is coupled to the assembly of complete flagella. Mutations which disrupt this coupling define a gene, flgM, which represses the expression of the...
DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium.
Park, U E, Olivera, B M, Hughes, K T, Roth, J R, Hillyard, D R
Bacterial DNA ligases use NAD as an energy source. In this study we addressed two questions about these enzymes. First, what is the physiological consequence of completely removing the NAD-dependent...
Hughes, K T, Simons, R W, Nunn, W D
Localized mutagenesis of the fadR region of the Escherichia coli chromosome resulted in the isolation of two classes of fadR regulatory mutants. The first class was constitutive for the fatty acid...
Structural gene for NAD synthetase in Salmonella typhimurium.
Hughes, K T, Olivera, B M, Roth, J R
We have identified the structural gene for NAD synthetase, which catalyzes the final metabolic step in NAD biosynthesis. This gene, designated nadE, is located between gdh and nit at 27 min on the...
Rec dependence of mu transposition from P22-transduced fragments.
Hughes, K T, Olivera, B M, Roth, J R
Derivatives of bacteriophage Mu carrying a lac operon and a selectable drug resistance element (Mu d phages) are frequently used tools of bacterial genetics. Mu d prophages used in this way can be...
Subunit-specific phenotypes of Salmonella typhimurium HU mutants.
Hillyard, D R, Edlund, M, Hughes, K T, Marsh, M, Higgins, N P
Salmonella hupA and hupB mutants were studied to determine the reasons for the high degree of conservation in HU structure in bacteria. We found one HU-1-specific effect; the F'128 plasmid was...
Conditionally transposition-defective derivative of Mu d1(Amp Lac).
A Mu d1 derivative is described which is useful for genetic manipulation of Mu-lac fusion insertions. A double mutant of the specialized transducing phage Mu d1(Amp Lac c62ts) was isolated which is...
6-Aminonicotinamide-resistant mutants of Salmonella typhimurium.
Hughes, K T, Cookson, B T, Ladika, D, Olivera, B M, Roth, J R
Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. (i) pncX mutants have Tn10 insertion...
An indispensable gene for NAD biosynthesis in Salmonella typhimurium.
Hughes, K T, Ladika, D, Roth, J R, Olivera, B M
We have located the nadD locus between lip and leuS at 14 min on the Salmonella typhimurium chromosome, and we have shown it to be the structural gene for nicotinic acid mononucleotide...
Simons, R W, Hughes, K T, Nunn, W D
Strains stably merodiploid in the 25-min region of the chromosome of Escherichia coli were constructed and used in dominance tests between various wild-type and mutant alleles of the fadR gene....
Hughes, K T, Gaines, P C, Karlinsey, J E, Vinayak, R, Simon, M I
The Hin recombinase of Salmonella catalyzes a site-specific recombination event which leads to flagellar phase variation. Starting with a fully symmetrical recombination site, hixC, a set of 40...
A genetic complementation system is described in which the complementing components are close together in a single linear DNA fragment; the complementation situation is temporary. This system is...
A Genetic Characterization of the Nadc Gene of Salmonella Typhimurium
Hughes, K. T., Roth, J. R., Olivera, B. M.
The nadC gene of Salmonella encodes the pyridine biosynthetic enzyme PRPP-quinolinate phosphoribosyltransferase. Using a combination of genetic techniques, a deletion map for the Salmonella nadC gene...
The Hin recombinase catalyzes a site-specific recombination reaction that results in the reversible inversion of a 1-kbp segment of the Salmonella chromosome. The DNA inversion reaction catalyzed by...