K. Ubukata

Publication List Details

Period

1994 - 2009

Number

33

Co-Authors

Increased macrolide resistance of Mycoplasma pneumoniae in France directly detected in clinical specimens by real-time PCR and melting curve analysis (2009)

Peuchant, O., Ménard, A., Renaudin, H., Morozumi, M., Ubukata, K., Bébéar, C. M., ...

Objectives Mycoplasma pneumoniae is a common aetiological agent of community-acquired respiratory tract infections for which macrolides are the treatment of choice. In France, only two...

Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae.

Yamane, A, Nakano, H, Asahi, Y, Ubukata, K, Konno, M

We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the...

Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.

Ubukata, K, Nonoguchi, R, Song, M D, Matsuhashi, M, Konno, M

A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains...

In vitro activity of LJC10,627, a new carbapenem antibiotic with high stability to dehydropeptidase I.

Ubukata, K, Hikida, M, Yoshida, M, Nishiki, K, Furukawa, Y, Tashiro, K, ...

The in vitro activity of LJC10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. LJC10,627 had broad-spectrum activity against gram-positive and gram-negative clinical...

Cloning and expression of the norA gene for fluoroquinolone resistance in Staphylococcus aureus.

Ubukata, K, Itoh-Yamashita, N, Konno, M

The norA gene for fluoroquinolone resistance in Staphylococcus aureus TK2566 was cloned from chromosomal DNA into fluoroquinolone-susceptible Escherichia coli TG1. The resulting transformant, NY11,...

Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci.

Ubukata, K, Nonoguchi, R, Matsuhashi, M, Song, M D, Konno, M

Chromosomal BamHI DNA fragments containing both the mecA gene encoding the penicillin-binding protein responsible for methicillin resistance and the aadD gene encoding 4',4"-adenylyltransferase...

Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci.

Ubukata, K, Yamashita, N, Konno, M

The mechanism of methicillin resistance was investigated in methicillin-resistant staphylococci (MRS) and in variants which had lost methicillin resistance. Phase-contrast microscopy showed that...

Purification and characterization of aminoglycoside-modifying enzymes from Staphylococcus aureus and Staphylococcus epidermidis.

Ubukata, K, Yamashita, N, Gotoh, A, Konno, M

Several strains of Staphylococcus aureus and Staphylococcus epidermidis, exhibiting characteristic resistance patterns to aminoglycoside antibiotics, were examined. The aminoglycoside-modifying...

Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

Ubukata, K, Nonoguchi, R, Matsuhashi, M, Konno, M

A beta-lactam-sensitive strain of Staphylococcus aureus could be converted to methicillin resistance by the introduction of a plasmid carrying the 4.3-kilobase HindIII chromosomal DNA fragment which...

Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones.

Yoshida, H, Bogaki, M, Nakamura, S, Ubukata, K, Konno, M

The norA gene cloned from chromosomal DNA of quinolone-resistant Staphylococcus aureus TK2566 conferred relatively high resistance to hydrophilic quinolones such as norfloxacin, enoxacin, ofloxacin,...

Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.

Matsuhashi, M, Song, M D, Ishino, F, Wachi, M, Doi, M, Inoue, M, ...

A novel penicillin-binding protein, PBP-2' (Mr about 75,000), is known to be induced in excessively large amount by most beta-lactam compounds in cells of a clinically isolated strain of...

Combinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumoniae by PCR.

Ubukata, K, Asahi, Y, Yamane, A, Konno, M

PCR was used to identify penicillin resistance in 1,062 clinical isolates of Streptococcus pneumoniae. Three sets of primers were designed to amplify (i) a 240-bp fragment of the penicillin-binding...

Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products.

Ubukata, K, Nakagami, S, Nitta, A, Yamane, A, Kawakami, S, Sugiura, M, ...

In order to identify methicillin-resistant staphylococci from clinical sources with ease and reliability, enzymatic detection of polymerase chain reaction (ED-PCR) was applied. ED-PCR is based on the...

Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae.

Yamane, A, Nakano, H, Asahi, Y, Ubukata, K, Konno, M

We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the...

Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.

Ubukata, K, Nonoguchi, R, Song, M D, Matsuhashi, M, Konno, M

A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains...

In vitro activity of LJC10,627, a new carbapenem antibiotic with high stability to dehydropeptidase I.

Ubukata, K, Hikida, M, Yoshida, M, Nishiki, K, Furukawa, Y, Tashiro, K, ...

The in vitro activity of LJC10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. LJC10,627 had broad-spectrum activity against gram-positive and gram-negative clinical...

Cloning and expression of the norA gene for fluoroquinolone resistance in Staphylococcus aureus.

Ubukata, K, Itoh-Yamashita, N, Konno, M

The norA gene for fluoroquinolone resistance in Staphylococcus aureus TK2566 was cloned from chromosomal DNA into fluoroquinolone-susceptible Escherichia coli TG1. The resulting transformant, NY11,...

Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci.

Ubukata, K, Nonoguchi, R, Matsuhashi, M, Song, M D, Konno, M

Chromosomal BamHI DNA fragments containing both the mecA gene encoding the penicillin-binding protein responsible for methicillin resistance and the aadD gene encoding 4',4"-adenylyltransferase...

Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci.

Ubukata, K, Yamashita, N, Konno, M

The mechanism of methicillin resistance was investigated in methicillin-resistant staphylococci (MRS) and in variants which had lost methicillin resistance. Phase-contrast microscopy showed that...

Purification and characterization of aminoglycoside-modifying enzymes from Staphylococcus aureus and Staphylococcus epidermidis.

Ubukata, K, Yamashita, N, Gotoh, A, Konno, M

Several strains of Staphylococcus aureus and Staphylococcus epidermidis, exhibiting characteristic resistance patterns to aminoglycoside antibiotics, were examined. The aminoglycoside-modifying...

Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

Ubukata, K, Nonoguchi, R, Matsuhashi, M, Konno, M

A beta-lactam-sensitive strain of Staphylococcus aureus could be converted to methicillin resistance by the introduction of a plasmid carrying the 4.3-kilobase HindIII chromosomal DNA fragment which...

Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones.

Yoshida, H, Bogaki, M, Nakamura, S, Ubukata, K, Konno, M

The norA gene cloned from chromosomal DNA of quinolone-resistant Staphylococcus aureus TK2566 conferred relatively high resistance to hydrophilic quinolones such as norfloxacin, enoxacin, ofloxacin,...

Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.

Matsuhashi, M, Song, M D, Ishino, F, Wachi, M, Doi, M, Inoue, M, ...

A novel penicillin-binding protein, PBP-2' (Mr about 75,000), is known to be induced in excessively large amount by most beta-lactam compounds in cells of a clinically isolated strain of...

Combinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumoniae by PCR.

Ubukata, K, Asahi, Y, Yamane, A, Konno, M

PCR was used to identify penicillin resistance in 1,062 clinical isolates of Streptococcus pneumoniae. Three sets of primers were designed to amplify (i) a 240-bp fragment of the penicillin-binding...

Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products.

Ubukata, K, Nakagami, S, Nitta, A, Yamane, A, Kawakami, S, Sugiura, M, ...

In order to identify methicillin-resistant staphylococci from clinical sources with ease and reliability, enzymatic detection of polymerase chain reaction (ED-PCR) was applied. ED-PCR is based on the...