Y. Fan, J. N. Weinstein, K. W. Kohn, L. M. Shi, Y. Pommier
This article not subject to U.S. Copyright. Published 1998 by the American Chemical Society
Pommier, Y, Kohlhagen, G, Kohn, K W, Leteurtre, F, Wani, M C, Wall, M E
DNA topoisomerase I (top1) is a ubiquitous nuclear enzyme. It is specifically inhibited by camptothecin, a natural product derived from the bark of the tree Camptotheca acuminata. Camptothecin and...
Role of the cdc25C phosphatase in G2 arrest induced by nitrogen mustard.
O'Connor, P M, Ferris, D K, Hoffmann, I, Jackman, J, Draetta, G, Kohn, K W
G2 arrest induced by nitrogen mustard in human lymphoma CA46 cells is associated with a failure to activate hyperphosphorylated cdc2/cyclin B1 complexes. We investigated the possibility that this...
Inhibitors of human immunodeficiency virus integrase.
Fesen, M R, Kohn, K W, Leteurtre, F, Pommier, Y
In an effort to further extend the number of targets for development of antiretroviral agents, we have used an in vitro integrase assay to investigate a variety of chemicals, including topoisomerase...
Pommier, Y, Cockerill, P N, Kohn, K W, Garrard, W T
We demonstrate that the simian virus 40 genome contains a single MAR (matrix association region) that maps within a large T-antigen coding region (nucleotides 4071 to 4377). This region contains...
Tanizawa, A, Kohn, K W, Pommier, Y
In this study, we further examined the sequence selectivity of camptothecin in mammalian topoisomerase I cDNA from human and Chinese hamster. In the absence of camptothecin, almost all the bases at...
This work presents a neutral filter elution method for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay will detect the number of double strand breaks induced by as...
Pommier, Y, Capranico, G, Orr, A, Kohn, K W
Several classes of antitumor drugs are known to stabilize topoisomerase complexes in which the enzyme is covalently bound to a terminus of a DNA strand break. The DNA cleavage sites generally are...
Capranico, G, Jaxel, C, Roberge, M, Kohn, K W, Pommier, Y
We have assessed the ability of nucleosomes to influence the formation of mammalian topoisomerase II-DNA complexes by mapping the sites of cleavage induced by four unrelated topoisomerase II...
Capranico, G, Kohn, K W, Pommier, Y
Doxorubicin, a DNA-intercalator, is one of several anti-cancer drugs that have been found to stabilizes topoisomerase II cleavage complexes at drug-specific DNA sites. The distribution and DNA...
Fornace, A J, Kohn, K W, Kann, H E
The method of DNA alkaline elution was applied to a study of the formation and resealing of DNA single-strand breaks after irradiation of human fibroblasts with ultraviolet light (UV). The general...
Topoisomerase I interaction with SV40 DNA in the presence and absence of camptothecin.
Jaxel, C, Kohn, K W, Pommier, Y
Camptothecin is an antitumor drug, which is a specific inhibitor of eukaryotic topoisomerase I. Enzyme inhibition is related to the stabilization of cleavable complexes between topoisomerase I and...
DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustards.
Mattes, W B, Hartley, J A, Kohn, K W
Nitrogen mustards alkylate DNA primarily at the N7 position of guanine. Using an approach analogous to that of the Maxam-Gilbert procedure for DNA sequence analysis, we have examined the relative...
Mechanisms of DNA sequence selective alkylation of guanine-N7 positions by nitrogen mustards.
Kohn, K W, Hartley, J A, Mattes, W B
Quantitative determinations were carried out of the relative reaction rates of several nitrogen mustards at various guanine-N7 positions in DNA fragments of known sequence. The findings suggest...
Erickson, L C, Bradley, M O, Ducore, J M, Ewig, R A, Kohn, K W
Normal (IMR-90) and simian virus 40-transformed (VA-13) human embryo cells were treated with antitumor nitrosoureas, and the effects on cell viability and cell DNA were compared. All six nitrosoureas...
Zhan, Q, Lord, K A, Alamo, I, Hollander, M C, Carrier, F, Ron, D, ...
A remarkable overlap was observed between the gadd genes, a group of often coordinately expressed genes that are induced by genotoxic stress and certain other growth arrest signals, and the MyD...
Fluorescent light-induced DNA crosslinkage and chromatid breaks in mouse cells in culture.
Gantt, R, Parshad, R, Ewig, R A, Sanford, K K, Jones, G M, Tarone, R E, ...
A single 20-hr exposure of mouse cells derived from embryonic or lung tissue to cool-white fluorescent light (4.6 W/m2) causes both DNA damage and chromosome aberrations including chromatid breaks,...
Shao, R G, Cao, C X, Zhang, H, Kohn, K W, Wold, M S, Pommier, Y
Replication protein A (RPA) is a DNA single-strand binding protein essential for DNA replication, recombination and repair. In human cells treated with the topoisomerase inhibitors camptothecin or...
Pommier, Y, Kohlhagen, G, Kohn, K W, Leteurtre, F, Wani, M C, Wall, M E
DNA topoisomerase I (top1) is a ubiquitous nuclear enzyme. It is specifically inhibited by camptothecin, a natural product derived from the bark of the tree Camptotheca acuminata. Camptothecin and...
Role of the cdc25C phosphatase in G2 arrest induced by nitrogen mustard.
O'Connor, P M, Ferris, D K, Hoffmann, I, Jackman, J, Draetta, G, Kohn, K W
G2 arrest induced by nitrogen mustard in human lymphoma CA46 cells is associated with a failure to activate hyperphosphorylated cdc2/cyclin B1 complexes. We investigated the possibility that this...
Inhibitors of human immunodeficiency virus integrase.
Fesen, M R, Kohn, K W, Leteurtre, F, Pommier, Y
In an effort to further extend the number of targets for development of antiretroviral agents, we have used an in vitro integrase assay to investigate a variety of chemicals, including topoisomerase...
Pommier, Y, Cockerill, P N, Kohn, K W, Garrard, W T
We demonstrate that the simian virus 40 genome contains a single MAR (matrix association region) that maps within a large T-antigen coding region (nucleotides 4071 to 4377). This region contains...
Tanizawa, A, Kohn, K W, Pommier, Y
In this study, we further examined the sequence selectivity of camptothecin in mammalian topoisomerase I cDNA from human and Chinese hamster. In the absence of camptothecin, almost all the bases at...
This work presents a neutral filter elution method for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay will detect the number of double strand breaks induced by as...
Pommier, Y, Capranico, G, Orr, A, Kohn, K W
Several classes of antitumor drugs are known to stabilize topoisomerase complexes in which the enzyme is covalently bound to a terminus of a DNA strand break. The DNA cleavage sites generally are...
Capranico, G, Jaxel, C, Roberge, M, Kohn, K W, Pommier, Y
We have assessed the ability of nucleosomes to influence the formation of mammalian topoisomerase II-DNA complexes by mapping the sites of cleavage induced by four unrelated topoisomerase II...
Capranico, G, Kohn, K W, Pommier, Y
Doxorubicin, a DNA-intercalator, is one of several anti-cancer drugs that have been found to stabilizes topoisomerase II cleavage complexes at drug-specific DNA sites. The distribution and DNA...
Fornace, A J, Kohn, K W, Kann, H E
The method of DNA alkaline elution was applied to a study of the formation and resealing of DNA single-strand breaks after irradiation of human fibroblasts with ultraviolet light (UV). The general...
Topoisomerase I interaction with SV40 DNA in the presence and absence of camptothecin.
Jaxel, C, Kohn, K W, Pommier, Y
Camptothecin is an antitumor drug, which is a specific inhibitor of eukaryotic topoisomerase I. Enzyme inhibition is related to the stabilization of cleavable complexes between topoisomerase I and...
DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustards.
Mattes, W B, Hartley, J A, Kohn, K W
Nitrogen mustards alkylate DNA primarily at the N7 position of guanine. Using an approach analogous to that of the Maxam-Gilbert procedure for DNA sequence analysis, we have examined the relative...
Mechanisms of DNA sequence selective alkylation of guanine-N7 positions by nitrogen mustards.
Kohn, K W, Hartley, J A, Mattes, W B
Quantitative determinations were carried out of the relative reaction rates of several nitrogen mustards at various guanine-N7 positions in DNA fragments of known sequence. The findings suggest...
Erickson, L C, Bradley, M O, Ducore, J M, Ewig, R A, Kohn, K W
Normal (IMR-90) and simian virus 40-transformed (VA-13) human embryo cells were treated with antitumor nitrosoureas, and the effects on cell viability and cell DNA were compared. All six nitrosoureas...
Zhan, Q, Lord, K A, Alamo, I, Hollander, M C, Carrier, F, Ron, D, ...
A remarkable overlap was observed between the gadd genes, a group of often coordinately expressed genes that are induced by genotoxic stress and certain other growth arrest signals, and the MyD...
Fluorescent light-induced DNA crosslinkage and chromatid breaks in mouse cells in culture.
Gantt, R, Parshad, R, Ewig, R A, Sanford, K K, Jones, G M, Tarone, R E, ...
A single 20-hr exposure of mouse cells derived from embryonic or lung tissue to cool-white fluorescent light (4.6 W/m2) causes both DNA damage and chromosome aberrations including chromatid breaks,...