Doi, Y., Adams, J.M., Yamane, K., Paterson, D.L.
Five highly amikacin-resistant Acinetobacter baumannii isolates were collected at a medical center in Pennsylvania. The aminoglycoside resistance was due to the production of the 16S rRNA methylase...
Doi, Y., Adams, J.M., Yamane, K., Paterson, D.L.
Five highly amikacin-resistant Acinetobacter baumannii isolates were collected at a medical center in Pennsylvania. The aminoglycoside resistance was due to the production of the 16S rRNA methylase...
Ambipolar organic light emitting field effect transistors with modified asymmetric electrodes (2007)
Constitutively phosphorylated Smad3 interacts with Sp1 and p300 in scleroderma fibroblasts (2006)
Ihn, H., Yamane, K., Asano, Y., Jinnin, M., Tamaki, K.
Objective. To elucidate the role of transforming growth factor-β (TGF-β)/Smad signalling in the increased expression of the collagen gene in systemic sclerosis (SSc) fibroblasts. Methods....
Asano, Y., Ihn, H., Asashima, N., Yazawa, N., Mimura, Y., Jinnin, M., ...
Asano, Y., Ihn, H., Asashima, N., Yazawa, N., Mimura, Y., Jinnin, M., ...
Constitutively phosphorylated Smad3 interacts with Sp1 and p300 in scleroderma fibroblasts (2005)
Ihn, H., Yamane, K., Asano, Y., Jinnin, M., Tamaki, K.
Objective. To elucidate the role of transforming growth factor-β (TGF-β)/Smad signalling in the increased expression of the collagen gene in systemic sclerosis (SSc) fibroblasts. Methods. Dermal...
Jinnin, M., Ihn, H., Yamane, K., Asano, Y., Yazawa, N., Tamaki, K.
Objective. To determine the frequency and clinical significance of plasma plasmin–α2‐plasmin inhibitor complex (PIC) in patients with systemic sclerosis (SSc). Methods....
Ihn, H., Asano, Y., Kubo, M., Yamane, K., Jinnin, M., Yazawa, N., ...
Objective. To determine the clinical significance of serum surfactant protein D (SP‐D) levels in patients with polymyositis/dermatomyositis (PM/DM). Methods. Serum...
Anti-U5 snRNP antibody as a possible serological marker for scleroderma-polymyositis overlap (2002)
Kubo, M., Ihn, H., Kuwana, M., Asano, Y., Tamaki, T., Yamane, K., ...
Objective. To determine the prevalence of the anti‐U5 small nuclear ribonucleoprotein (snRNP) antibody in patients with systemic sclerosis. Methods. Sera from 281 patients with...
Absorption-free optical control of spin systems:the quantum Zeno effect in optical pumping (2001)
Nakanishi, T., Yamane, K., Kitano, M.
We show that atomic spin motion can be controlled by circularly polarized light without light absorption in the strong pumping limit. In this limit, the pumping light, which drives the empty spin...
Antibodies to Th/To ribonucleoprotein in patients with localized scleroderma (2001)
Yamane, K., Ihn, H., Kubo, M., Kuwana, M., Asano, Y., Yazawa, N., ...
Objective. Antibodies to Th/To ribonucleoprotein (anti‐Th/To) have been detected almost exclusively in patients with systemic sclerosis. Therefore, we aimed to determine...
Clinical and laboratory features of scleroderma patients with pulmonary hypertension (2000)
Yamane, K., Ihn, H., Asano, Y., Yazawa, N., Kubo, M., Kikuchi, K., ...
Objective. Pulmonary hypertension (PH) is a frequent cause of death in patients with systemic sclerosis (SSc). In this study, we examined the occurrence of PH and investigated...
Serum KL-6 in adult patients with polymyositis and dermatomyositis (2000)
Kubo, M., Ihn, H., Yamane, K., Kikuchi, K., Yazawa, N., Soma, Y., ...
Objective. To determine the serum levels of KL‐6, a mucin‐like high‐molecular‐weight glycoprotein, in polymyositis/dermatomyositis (PM/DM) patients....
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis (1997)
Kunst, F., Ogasawara, N., Moszer, I., Albertini, A.M., Alloni, G., Azevedo, V., ...
Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are...
The Complete Genome Sequence Of The Gram-Positive Bacterium Bacillus Subtilis (1997)
Kunst, F., Ogasawara, N., Moszer, I., Albertini, Am., Alloni, G., Azevedo, V., ...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
Ogawa, K, Akagawa, E, Yamane, K, Sun, Z W, LaCelle, M, Zuber, P, ...
Bacillus subtilis can use either nitrate or nitrite as a sole source of nitrogen. The isolation of the nasABCDEF genes of B. subtilis, which are required for nitrate/nitrite assimilation, is...
Sakakibara, Y, Tsutsumi, K, Nakamura, K, Yamane, K
The Bacillus subtilis alpha-amylase signal peptide consists of 33 amino acids from its translation initiation site. To analyze the structural requirements for efficient processing of the signal...
Honda, K, Nakamura, K, Nishiguchi, M, Yamane, K
By using a DNA fragment of Escherichia coli ffh as a probe, the Bacillus subtilis ffh gene was cloned. The complete nucleotide sequence of the cloned DNA revealed that it contained three open reading...
Nishiguchi, M, Honda, K, Amikura, R, Nakamura, K, Yamane, K
Bacillus subtilis small cytoplasmic RNA (scRNA; 271 nucleotides) is a member of the signal recognition particle (SRP) RNA family, which has evolutionarily conserved primary and secondary structures....
Nakamura, K, Imai, Y, Nakamura, A, Yamane, K
Small cytoplasmic RNA (scRNA; 271 nucleotides) is an abundant and stable RNA of the gram-positive bacterium Bacillus subtilis. To investigate the function of scRNA in B. subtilis cells, we developed...
In vivo and in vitro characterization of the secA gene product of Bacillus subtilis.
Takamatsu, H, Fuma, S, Nakamura, K, Sadaie, Y, Shinkai, A, Matsuyama, S, ...
The putative amino acid sequence from the wild-type Bacillus subtilis div+ gene, which complements the temperature-sensitive div-341 mutation, shares a 50% identity with the sequence from Escherichia...
Kimura, K, Kataoka, S, Ishii, Y, Takano, T, Yamane, K
The nucleotide sequence of the gene for cyclodextrin glucanotransferase of alkalophilic Bacillus sp. strain 1011 was determined. The deduced amino acid sequence at the NH2-terminal side of the enzyme...
Furusato, T, Takano, J, Yamane, K, Hashiguchi, K, Tanimoto, A, Mori, M, ...
A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The...
Yamazaki, H, Ohmura, K, Nakayama, A, Takeichi, Y, Otozai, K, Yamasaki, M, ...
amyR2, amyE+, and aroI+ alleles from an alpha-amylase-hyperproducing strain, Bacillus subtilis NA64, were cloned in temperate B. subtilis phage p11, and the amyR2 and amyE+ genes were then recloned...
Takano, T, Fukuda, M, Monma, M, Kobayashi, S, Kainuma, K, Yamane, K
The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from...
Uehara, H, Yamane, K, Maruo, B
Two mutants (NT02 and NT17), each producing a thermosensitive neutral protease, were isolated from Bacillus subtilis NP58, a transformant which acquired the property of hyperproduction of neutral...
Tunicamycin-resistant mutants and chromosomal locations of mutational sites in Bacillus subtilis.
Nomura, S, Yamane, K, Sasaki, T, Yamasaki, M, Tamura, G, Maruo, B
The types of tunicamycin-resistant mutants of Bacillus subtilis were analyzed, and their mutational sites on the chromosome were mapped. A type 1 mutation that simultaneously expressed...
A membrane-bound insoluble alkaline phosphatase (APase) and an extracellular soluble APase were purified, respectively, from a membrane preparation of Bacillus subtilis 6160-BC6, which carries a...
In Bacillus subtilis Marburg strain, single-point mutations in the phoP locus brought about simultaneous losses of the major activities of alkaline phosphatase (APase) and alkaline phosphodiesterase...
Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus subtilis.
LaCelle, M, Kumano, M, Kurita, K, Yamane, K, Zuber, P, Nakano, M M
A gene, hmp, which encodes a ubiquitous protein homologous to hemoglobin was isolated among genes from Bacillus subtilis that are induced under anaerobic conditions. The hmp protein belongs to the...
Ayusawa, D, Yoneda, Y, Yamane, K, Maruo, B
A mutant of Bacillus subtilis 6160 that had been isolated by its hyperproduction of alpha-amylase and protease lacked flagella and motility, and its content of autolytic enzyme(s) was reduced to...
Yoneda, Y., Yamane, K., Yamaguchi, K., Nagata, Y., Maruo, B.
Deoxyribonucleic acid (DNA) of Bacillus subtilis var. amylosacchariticus showed almost the same ability as B. subtilis Marburg to induce transfer of several genetic markers in DNA-mediated...
Fuma, S, Fujishima, Y, Corbell, N, D'Souza, C, Nakano, M M, Zuber, P, ...
The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region,...
Ohmura, K, Nakamura, K, Yamazaki, H, Shiroza, T, Yamane, K, Jigami, Y, ...
The precursor of Bacillus subtilis alpha-amylase contains an NH2-terminal extension of 41 amino acid residues as the signal sequence. The E. coli beta-lactamase structural gene was fused with the DNA...
Isolation and Properties of RNA Replicases Induced by SP and FI Phages
Haruna, I., Itoh, Y. H., Yamane, K., Miyake, T., Shiba, T., Watanabe, I.
New RNA replicases were isolated and purified from Escherichia coli Q13 infected with SP or FI phages showing different serological properties. These replicases showed a template specificity...
Grouping of RNA Phages Based on the Template Specificity of Their RNA Replicases
Miyake, T., Haruna, I., Shiba, T., Itoh, Y. H., Yamane, K., Watanabe, I.
We attempted to apply a new criterion, the template specificity of RNA replicases of Escherichia coli RNA phages, for the grouping of these phages. Based on the template specificity, it was shown...
Kawabe, A, Yamane, K, Miyashita, N T
DNA variation in a 4.7-kb region of the cytosolic phosphoglucose isomerase (PgiC) locus was investigated for 21 ecotypes of Arabidopsis thaliana. The estimated nucleotide diversity was 0.0038, which...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
Ogawa, K, Akagawa, E, Yamane, K, Sun, Z W, LaCelle, M, Zuber, P, ...
Bacillus subtilis can use either nitrate or nitrite as a sole source of nitrogen. The isolation of the nasABCDEF genes of B. subtilis, which are required for nitrate/nitrite assimilation, is...
Sakakibara, Y, Tsutsumi, K, Nakamura, K, Yamane, K
The Bacillus subtilis alpha-amylase signal peptide consists of 33 amino acids from its translation initiation site. To analyze the structural requirements for efficient processing of the signal...
Honda, K, Nakamura, K, Nishiguchi, M, Yamane, K
By using a DNA fragment of Escherichia coli ffh as a probe, the Bacillus subtilis ffh gene was cloned. The complete nucleotide sequence of the cloned DNA revealed that it contained three open reading...
Nishiguchi, M, Honda, K, Amikura, R, Nakamura, K, Yamane, K
Bacillus subtilis small cytoplasmic RNA (scRNA; 271 nucleotides) is a member of the signal recognition particle (SRP) RNA family, which has evolutionarily conserved primary and secondary structures....
Nakamura, K, Imai, Y, Nakamura, A, Yamane, K
Small cytoplasmic RNA (scRNA; 271 nucleotides) is an abundant and stable RNA of the gram-positive bacterium Bacillus subtilis. To investigate the function of scRNA in B. subtilis cells, we developed...
In vivo and in vitro characterization of the secA gene product of Bacillus subtilis.
Takamatsu, H, Fuma, S, Nakamura, K, Sadaie, Y, Shinkai, A, Matsuyama, S, ...
The putative amino acid sequence from the wild-type Bacillus subtilis div+ gene, which complements the temperature-sensitive div-341 mutation, shares a 50% identity with the sequence from Escherichia...
Kimura, K, Kataoka, S, Ishii, Y, Takano, T, Yamane, K
The nucleotide sequence of the gene for cyclodextrin glucanotransferase of alkalophilic Bacillus sp. strain 1011 was determined. The deduced amino acid sequence at the NH2-terminal side of the enzyme...
Furusato, T, Takano, J, Yamane, K, Hashiguchi, K, Tanimoto, A, Mori, M, ...
A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The...
Yamazaki, H, Ohmura, K, Nakayama, A, Takeichi, Y, Otozai, K, Yamasaki, M, ...
amyR2, amyE+, and aroI+ alleles from an alpha-amylase-hyperproducing strain, Bacillus subtilis NA64, were cloned in temperate B. subtilis phage p11, and the amyR2 and amyE+ genes were then recloned...
Takano, T, Fukuda, M, Monma, M, Kobayashi, S, Kainuma, K, Yamane, K
The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from...
Uehara, H, Yamane, K, Maruo, B
Two mutants (NT02 and NT17), each producing a thermosensitive neutral protease, were isolated from Bacillus subtilis NP58, a transformant which acquired the property of hyperproduction of neutral...
Tunicamycin-resistant mutants and chromosomal locations of mutational sites in Bacillus subtilis.
Nomura, S, Yamane, K, Sasaki, T, Yamasaki, M, Tamura, G, Maruo, B
The types of tunicamycin-resistant mutants of Bacillus subtilis were analyzed, and their mutational sites on the chromosome were mapped. A type 1 mutation that simultaneously expressed...
A membrane-bound insoluble alkaline phosphatase (APase) and an extracellular soluble APase were purified, respectively, from a membrane preparation of Bacillus subtilis 6160-BC6, which carries a...
In Bacillus subtilis Marburg strain, single-point mutations in the phoP locus brought about simultaneous losses of the major activities of alkaline phosphatase (APase) and alkaline phosphodiesterase...
Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus subtilis.
LaCelle, M, Kumano, M, Kurita, K, Yamane, K, Zuber, P, Nakano, M M
A gene, hmp, which encodes a ubiquitous protein homologous to hemoglobin was isolated among genes from Bacillus subtilis that are induced under anaerobic conditions. The hmp protein belongs to the...
Ayusawa, D, Yoneda, Y, Yamane, K, Maruo, B
A mutant of Bacillus subtilis 6160 that had been isolated by its hyperproduction of alpha-amylase and protease lacked flagella and motility, and its content of autolytic enzyme(s) was reduced to...
Yoneda, Y., Yamane, K., Yamaguchi, K., Nagata, Y., Maruo, B.
Deoxyribonucleic acid (DNA) of Bacillus subtilis var. amylosacchariticus showed almost the same ability as B. subtilis Marburg to induce transfer of several genetic markers in DNA-mediated...
Fuma, S, Fujishima, Y, Corbell, N, D'Souza, C, Nakano, M M, Zuber, P, ...
The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region,...
Ohmura, K, Nakamura, K, Yamazaki, H, Shiroza, T, Yamane, K, Jigami, Y, ...
The precursor of Bacillus subtilis alpha-amylase contains an NH2-terminal extension of 41 amino acid residues as the signal sequence. The E. coli beta-lactamase structural gene was fused with the DNA...
Isolation and Properties of RNA Replicases Induced by SP and FI Phages
Haruna, I., Itoh, Y. H., Yamane, K., Miyake, T., Shiba, T., Watanabe, I.
New RNA replicases were isolated and purified from Escherichia coli Q13 infected with SP or FI phages showing different serological properties. These replicases showed a template specificity...
Grouping of RNA Phages Based on the Template Specificity of Their RNA Replicases
Miyake, T., Haruna, I., Shiba, T., Itoh, Y. H., Yamane, K., Watanabe, I.
We attempted to apply a new criterion, the template specificity of RNA replicases of Escherichia coli RNA phages, for the grouping of these phages. Based on the template specificity, it was shown...
Kawabe, A, Yamane, K, Miyashita, N T
DNA variation in a 4.7-kb region of the cytosolic phosphoglucose isomerase (PgiC) locus was investigated for 21 ecotypes of Arabidopsis thaliana. The estimated nucleotide diversity was 0.0038, which...
Anti-U3 snRNP antibodies in localised scleroderma
YAMANE, K, IHN, H, KUBO, M, ASANO, Y, YAZAWA, N, TAMAKI, K, ...
Effect of D-penicillamine on pulmonary fibrosis in patients with systemic sclerosis
Jinnin, M, Ihn, H, Asano, Y, Yamane, K, Yazawa, N, Tamaki, K
Circulating soluble CD40 ligand in patients with eosinophilic fasciitis
Jinnin, M, Ihn, H, Yazawa, N, Asano, Y, Yamane, K, Tamaki, K
Suzuki, H., Shibano, K., Okane, M., Kono, I., Matsui, Y., Yamane, K., ...
The authors have investigated the effects of cytokines and lipopolysaccharide (LPS) on mRNA levels of c-sis (platelet-derived growth factor (PDGF)-B chain), PDGF-A chain, and interleukin 1 beta (IL-1...
Kono, I, Sakurai, T, Kabashima, T, Yamane, K, Kashiwagi, H
Fibronectin and C1q frequently co-precipitated in cryoglobulins from patients with SLE. As a portion of the C1q molecule is similar to collagen to which fibronectin has a high affinity, we studied...
Complement-mediated solubilization of immune complexes in systemic lupus erythematosus.
Sakurai, T, Fujita, T, Kono, I, Kabashima, T, Yamane, K, Tamura, N, ...
The capacity of complement-mediated solubilization of immune complexes (complex releasing activity: CRA) was studied in 63 sera from eight systemic lupus erythematosus (SLE) patients. CRA in sera of...
Distribution and antigen specificity of anti-U1RNP antibodies in patients with systemic sclerosis
Ihn, H, Yamane, K, Yazawa, N, Kubo, M, Fujimoto, M, Sato, S, ...
Systemic sclerosis (SSc) is a generalized connective tissue disease which is characterized by the presence of several autoantibodies. To determine the prevalence and antigen specificity of anti-U1RNP...
Kubo, M, Ihn, H, Kuwana, M, Yamane, K, Yazawa, N, Tamaki, K
New antibodies against a U3 snRNP, which were named anti-Myo 22/25 antibodies, were detected in four (8%) of 53 serum samples from patients with polymyositis/dermatomyositis (PM/DM) by RNA...
YAMANE, K, YAMAMOTO, K, YOSHIKAWA, Y, SASAZUKI, T
Previous studies have shown that a transgenic I-Eα gene, the mouse homologue of human DRα gene, prevents the development of insulitis and hence of diabetes in NOD mice. To investigate the mechanism...