Scientific Commons: Kanji Hirai

Identification of Minimal oriP of Epstein-Barr Virus Required for DNA Replication (1998)

DNA replication from oriP of Epstein-Barr virus is mediated by the virus replication factor EBNA1 and cellular factors and occurs approximately once in each cell cycle. We have identified a minimal...

Detection of Epstein-Barr virus DNA in virus-infected cells by electron microscopic in situ hybridization (1997)

Goto, Toshiyuki, Kohno, Takehiro, Hong, Wu, Hironaka, Takashi, Hirai, Kanji, Nakai, Masuyo

We describe a procedure for in situ hybridization using a biotinylated Epstein-Barr virus (EBV) sequence with detection at the light and electron microscopic levels. In situ hybridization using an...

Evaluation of DNA Homology of Marek's Disease Virus, Herpesvirus of Turkeys and Epstein-Barr Virus under Varied Stringent Hybridization Conditions (1984)

HIRAI, Kanji, IKUTA, Kazuyoshi, MAOTANI, Kumiko, KATO, Shiro

Marek's disease virus (MDV) showed only 0.3–0.6% homology in DNA sequence with herpesvirus of turkeys (HVT) at Tm-24.4�C in spite of its antigenic similarity to the latter. Southern blot...

Herpes simplex virus 1 α regulatory protein ICP0 functionally interacts with cellular transcription factor BMAL1

Kawaguchi, Yasushi, Tanaka, Michiko, Yokoymama, Akihiko, Matsuda, Go, Kato, Kentaro, Kagawa, Hiroyuki, ...

The infected cell protein no. 0 (ICP0) of herpes simplex virus 1 (HSV-1) is a promiscuous transactivator shown to enhance the expression of gene introduced into cells by infection or transfection. At...

Interaction of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP) with HS1-Associated Protein X-1: Implication of Cytoplasmic Function of EBNA-LP

Kawaguchi, Yasushi, Nakajima, Kaori, Igarashi, Mie, Morita, Tomoko, Tanaka, Michiko, Suzuki, Mikiko, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) consists of W1W2 repeats and a unique C-terminal Y1Y2 domain and has been suggested to play an important role in EBV-induced...

Cellular Elongation Factor 1δ Is Modified in Cells Infected with Representative Alpha-, Beta-, or Gammaherpesviruses

Kawaguchi, Yasushi, Matsumura, Tomio, Roizman, Bernard, Hirai, Kanji

Earlier reports (Y. Kawaguchi, R. Bruni, and B. Roizman, J. Virol. 71:1019–1024, 1997; Y. Kawaguchi, C. Van Sant, and B. Roizman, J. Virol. 72:1731–1736, 1998) showed that herpes simplex virus 1...

Establishment and Characterization of a Human Epstein-Barr Virus-Associated Gastric Carcinoma in SCID Mice

Iwasaki, Yoshiaki, Chong, Ja-Mun, Hayashi, Yukiko, Ikeno, Rie, Arai, Kuniyoshi, Kitamura, Masatsugu, ...

A transplantable human Epstein-Barr virus-associated gastric carcinoma (EBVaGC), designated KT, was propagated in severe combined immunodeficiency (SCID) mice for 12 passages. Mucin and cytokeratin...

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

Sonoda, Kengo, Sakaguchi, Masashi, Okamura, Hiroshi, Yokogawa, Kenji, Tokunaga, Eiji, Tokiyoshi, Sachio, ...

An earlier report (M. Sakaguchi et al., Vaccine 16:472–479, 1998) showed that recombinant Marek's disease virus type 1 (rMDV1) expressing the fusion (F) protein of Newcastle disease virus (NDV-F)...

Activation of TRAF5 and TRAF6 Signal Cascades Negatively Regulates the Latent Replication Origin of Epstein-Barr Virus through p38 Mitogen-Activated Protein Kinase

Shirakata, Masaki, Imadome, Ken-Ichi, Okazaki, Kenji, Hirai, Kanji

Latent Epstein-Barr virus (EBV) is maintained by the virus replication origin oriP that initiates DNA replication with the viral oriP-binding factor EBNA1. However, it is not known whether oriP's...

Identification of Major Phosphorylation Sites of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP): Ability of EBNA-LP To Induce Latent Membrane Protein 1 Cooperatively with EBNA-2 Is Regulated by Phosphorylation†

Yokoyama, Akihiko, Tanaka, Michiko, Matsuda, Go, Kato, Kentaro, Kanamori, Mikiko, Kawasaki, Hiroshi, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) is a phosphoprotein suggested to play important roles in EBV-induced immortalization of B cells. One of the potential functions of...

Conserved Region CR2 of Epstein-Barr Virus Nuclear Antigen Leader Protein Is a Multifunctional Domain That Mediates Self-Association as well as Nuclear Localization and Nuclear Matrix Association

Tanaka, Michiko, Yokoyama, Akihiko, Igarashi, Mie, Matsuda, Go, Kato, Kentaro, Kanamori, Mikiko, ...

Self-association of viral proteins is important for many of their functions, including enzymatic, transcriptional, and transformational activities. Epstein-Barr virus (EBV) nuclear antigen leader...

Novel Immediate-Early Protein IE19 of Human Cytomegalovirus Activates the Origin Recognition Complex I Promoter in a Cooperative Manner with IE72

Shirakata, Masaki, Terauchi, Masakazu, Ablikim, Melike, Imadome, Ken-Ichi, Hirai, Kanji, Aso, Takeshi, ...

The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated...