Karl Ekwall

The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres (2005)

Walfridsson, Julian, Bjerling, Pernilla, Thalen, Maria, Yoo, Eung-Jae, Park, Sang Dai, Ekwall, Karl

Centromeres of fission yeast are arranged with a central core DNA sequence flanked by repeated sequences. The centromere-associated histone H3 variant Cnp1 (SpCENP-A) binds exclusively to central...

RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing (2005)

Djupedal, Ingela, Portoso, Manuela, Spåhr, Henrik, Bonilla, Carolina, Gustafsson, Claes M., Allshire, Robin C., ...

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2...

A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift (2004)

Xue,Yongtao T., Haas,Stefan, Brino,Laurento, Gusnanto,Arief, Riemers,Mark, Talibi,Driss, ...

Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission...

A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift (2004)

Xue,Yongtao T., Haas,Stefan, Brino,Laurento, Gusnanto,Arief, Riemers,Mark, Talibi,Driss, ...

Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission...

A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift (2004)

Xue, Yongtao T., Haas, Stefan, Brino, Laurento, Gusnanto, Arief, Riemers, Mark, Talibi, Driss, ...

Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission...

A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift (2004)

Xue, Yongtao T., Haas, Stefan, Brino, Laurento, Gusnanto, Arief, Riemers, Mark, Talibi, Driss, ...

Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission...

A novel type of silencing factor, Clr2, is necessary for transcriptional silencing at various chromosomal locations in the fission yeast Schizosaccharomyces pombe (2004)

Bjerling, Pernilla, Ekwall, Karl, Egel, Richard, Thon, Geneviève

The mating-type region of the fission yeast Schizosaccharomyces pombe comprises three loci: mat1, mat2-P and mat3-M. mat1 is expressed and determines the mating type of the cell. mat2-P and mat3-M...

A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift (2003)

Xue,Yongtao T., Haas,Stefan, Brino,Laurento, Gusnanto,Arief, Riemers,Mark, Talibi,Driss, ...

Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission...

A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift (2003)

Xue, Yongtao T., Haas, Stefan, Brino, Laurento, Gusnanto, Arief, Riemers, Mark, Talibi, Driss, ...

Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission...

Fission yeast Hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation (2000)

Yoo, Eung Jae, Jin, Yong Hwan, Jang, Yeun Kyu, Bjerling, Pernilla, Tabish, Mohammad, Hong, Seung Hwan, ...

Hrp1 of Schizosaccharomyces pombe is a member of the CHD protein family, characterized by a chromodomain, a Myb-like telobox-related DNA-binding domain and a SNF2-related helicase/ATPase domain....

The silent P mating type locus in fission yeast contains two autonomously replicating sequences (1993)

Oisson, Tim, Ekwall, Karl, Ruusala, Tarmo

We show that in fission yeast two DNA fragments at the silent P mating type locus provide plasmids with the capability of autonomous replication. Bacterial vectors containing these sequences...

The Domain Structure of Centromeres Is Conserved from Fission Yeast to Humans

Kniola, Barbara, O'Toole, Eileen, McIntosh, J. Richard, Mellone, Barbara, Allshire, Robin, Mengarelli, Silwa, ...

The centromeric DNA of fission yeast is arranged with a central core flanked by repeated sequences. The centromere-associated proteins, Mis6p and Cnp1p (SpCENP-A), associate exclusively with central...

A New Member of the Sin3 Family of Corepressors Is Essential for Cell Viability and Required for Retroelement Propagation in Fission Yeast

Dang, Van Dinh, Benedik, Michael J., Ekwall, Karl, Choi, Jeannie, Allshire, Robin C., Levin, Henry L.

Tf1 is a long terminal repeat (LTR)-containing retrotransposon that propagates within the fission yeast Schizosaccharomyces pombe. LTR-retrotransposons possess significant similarity to retroviruses...

Fission yeast Hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation

Yoo, Eung Jae, Jin, Yong Hwan, Jang, Yeun Kyu, Bjerling, Pernilla, Tabish, Mohammad, Hong, Seung Hwan, ...

Hrp1 of Schizosaccharomyces pombe is a member of the CHD protein family, characterized by a chromodomain, a Myb-like telobox-related DNA-binding domain and a SNF2-related helicase/ATPase domain. CHD...

Functional Divergence between Histone Deacetylases in Fission Yeast by Distinct Cellular Localization and In Vivo Specificity

Bjerling, Pernilla, Silverstein, Rebecca A., Thon, Geneviève, Caudy, Amy, Grewal, Shiv, Ekwall, Karl

Histone deacetylases (HDACs) are important for gene regulation and the maintenance of heterochromatin in eukaryotes. Schizosaccharomyces pombe was used as a model system to investigate the functional...

Dicer is required for chromosome segregation and gene silencing in fission yeast cells

Provost, Patrick, Silverstein, Rebecca A., Dishart, David, Walfridsson, Julian, Djupedal, Ingela, Kniola, Barbara, ...

RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission...

Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity

Nakayama, Jun-ichi, Xiao, Guoping, Noma, Ken-ichi, Malikzay, Asra, Bjerling, Pernilla, Ekwall, Karl, ...

The post-translational modifications of histones are key to the modulation of chromatin structure. Distinct patterns of modifications established by histone-modifying enzymes control diverse...

Ago1 and Dcr1, Two Core Components of the RNA Interference Pathway, Functionally Diverge from Rdp1 in Regulating Cell Cycle Events in Schizosaccharomyces pombe

Carmichael, Jon B., Provost, Patrick, Ekwall, Karl, Hobman, Tom C.

In the fission yeast Schizosaccharomyces pombe, three genes that function in the RNA interference (RNAi) pathway, ago1+, dcr1+, and rdp1+, have recently been shown to be important for timely...

A novel type of silencing factor, Clr2, is necessary for transcriptional silencing at various chromosomal locations in the fission yeast Schizosaccharomyces pombe

Bjerling, Pernilla, Ekwall, Karl, Egel, Richard, Thon, Geneviève

The mating-type region of the fission yeast Schizosaccharomyces pombe comprises three loci: mat1, mat2-P and mat3-M. mat1 is expressed and determines the mating type of the cell. mat2-P and mat3-M...

The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres

Walfridsson, Julian, Bjerling, Pernilla, Thalen, Maria, Yoo, Eung-Jae, Park, Sang Dai, Ekwall, Karl

Centromeres of fission yeast are arranged with a central core DNA sequence flanked by repeated sequences. The centromere-associated histone H3 variant Cnp1 (SpCENP-A) binds exclusively to central...

Genomewide analysis of nucleosome density histone acetylation and HDAC function in fission yeast

Wirén, Marianna, Silverstein, Rebecca A, Sinha, Indranil, Walfridsson, Julian, Lee, Hang-mao, Laurenson, Patricia, ...

We have conducted a genomewide investigation into the enzymatic specificity, expression profiles, and binding locations of four histone deacetylases (HDACs), representing the three different...

RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing

Djupedal, Ingela, Portoso, Manuela, Spåhr, Henrik, Bonilla, Carolina, Gustafsson, Claes M., Allshire, Robin C., ...

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2...

The Domain Structure of Centromeres Is Conserved from Fission Yeast to Humans

Kniola, Barbara, O'Toole, Eileen, McIntosh, J. Richard, Mellone, Barbara, Allshire, Robin, Mengarelli, Silwa, ...

The centromeric DNA of fission yeast is arranged with a central core flanked by repeated sequences. The centromere-associated proteins, Mis6p and Cnp1p (SpCENP-A), associate exclusively with central...

A New Member of the Sin3 Family of Corepressors Is Essential for Cell Viability and Required for Retroelement Propagation in Fission Yeast

Dang, Van Dinh, Benedik, Michael J., Ekwall, Karl, Choi, Jeannie, Allshire, Robin C., Levin, Henry L.

Tf1 is a long terminal repeat (LTR)-containing retrotransposon that propagates within the fission yeast Schizosaccharomyces pombe. LTR-retrotransposons possess significant similarity to retroviruses...

Fission yeast Hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation

Yoo, Eung Jae, Jin, Yong Hwan, Jang, Yeun Kyu, Bjerling, Pernilla, Tabish, Mohammad, Hong, Seung Hwan, ...

Hrp1 of Schizosaccharomyces pombe is a member of the CHD protein family, characterized by a chromodomain, a Myb-like telobox-related DNA-binding domain and a SNF2-related helicase/ATPase domain. CHD...

Functional Divergence between Histone Deacetylases in Fission Yeast by Distinct Cellular Localization and In Vivo Specificity

Bjerling, Pernilla, Silverstein, Rebecca A., Thon, Geneviève, Caudy, Amy, Grewal, Shiv, Ekwall, Karl

Histone deacetylases (HDACs) are important for gene regulation and the maintenance of heterochromatin in eukaryotes. Schizosaccharomyces pombe was used as a model system to investigate the functional...

Dicer is required for chromosome segregation and gene silencing in fission yeast cells

Provost, Patrick, Silverstein, Rebecca A., Dishart, David, Walfridsson, Julian, Djupedal, Ingela, Kniola, Barbara, ...

RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission...

Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity

Nakayama, Jun-ichi, Xiao, Guoping, Noma, Ken-ichi, Malikzay, Asra, Bjerling, Pernilla, Ekwall, Karl, ...

The post-translational modifications of histones are key to the modulation of chromatin structure. Distinct patterns of modifications established by histone-modifying enzymes control diverse...

Ago1 and Dcr1, Two Core Components of the RNA Interference Pathway, Functionally Diverge from Rdp1 in Regulating Cell Cycle Events in Schizosaccharomyces pombe

Carmichael, Jon B., Provost, Patrick, Ekwall, Karl, Hobman, Tom C.

In the fission yeast Schizosaccharomyces pombe, three genes that function in the RNA interference (RNAi) pathway, ago1+, dcr1+, and rdp1+, have recently been shown to be important for timely...

A novel type of silencing factor, Clr2, is necessary for transcriptional silencing at various chromosomal locations in the fission yeast Schizosaccharomyces pombe

Bjerling, Pernilla, Ekwall, Karl, Egel, Richard, Thon, Geneviève

The mating-type region of the fission yeast Schizosaccharomyces pombe comprises three loci: mat1, mat2-P and mat3-M. mat1 is expressed and determines the mating type of the cell. mat2-P and mat3-M...

The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres

Walfridsson, Julian, Bjerling, Pernilla, Thalen, Maria, Yoo, Eung-Jae, Park, Sang Dai, Ekwall, Karl

Centromeres of fission yeast are arranged with a central core DNA sequence flanked by repeated sequences. The centromere-associated histone H3 variant Cnp1 (SpCENP-A) binds exclusively to central...

Genomewide analysis of nucleosome density histone acetylation and HDAC function in fission yeast

Wirén, Marianna, Silverstein, Rebecca A, Sinha, Indranil, Walfridsson, Julian, Lee, Hang-mao, Laurenson, Patricia, ...

We have conducted a genomewide investigation into the enzymatic specificity, expression profiles, and binding locations of four histone deacetylases (HDACs), representing the three different...

RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing

Djupedal, Ingela, Portoso, Manuela, Spåhr, Henrik, Bonilla, Carolina, Gustafsson, Claes M., Allshire, Robin C., ...

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2...

Genome-Wide Studies of Histone Demethylation Catalysed by the Fission Yeast Homologues of Mammalian LSD1

Opel, Michael, Lando, David, Bonilla, Carolina, Trewick, Sarah C., Boukaba, Abdelhalim, Walfridsson, Julian, ...

In order to gain a more global view of the activity of histone demethylases, we report here genome-wide studies of the fission yeast SWIRM and polyamine oxidase (PAO) domain homologues of mammalian...

Individual Subunits of the Ssn6-Tup11/12 Corepressor Are Selectively Required for Repression of Different Target Genes▿ †

Fagerström-Billai, Fredrik, Durand-Dubief, Mikaël, Ekwall, Karl, Wright, Anthony P. H.

The Saccharomyces cerevisiae Ssn6 and Tup1 proteins form a corepressor complex that is recruited to target genes by DNA-bound repressor proteins. Repression occurs via several mechanisms, including...

Interaction of Epe1 With the Heterochromatin Assembly Pathway in Schizosaccharomyces pombe

Isaac, Sara, Walfridsson, Julian, Zohar, Tal, Lazar, David, Kahan, Tamar, Ekwall, Karl, ...

Epe1 is a JmjC domain protein that antagonizes heterochromatization in Schizosaccharomyces pombe. Related JmjC domain proteins catalyze a histone demethylation reaction that depends on Fe(II) and...

A NASP (N1/N2)-Related Protein, Sim3, Binds CENP-A and Is Required for Its Deposition at Fission Yeast Centromeres

Dunleavy, Elaine M., Pidoux, Alison L., Monet, Marie, Bonilla, Carolina, Richardson, William, Hamilton, Georgina L., ...

A defining feature of centromeres is the presence of the histone H3 variant CENP-ACnp1. It is not known how CENP-ACnp1 is specifically delivered to, and assembled into, centromeric chromatin. Through...

The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics

Façanha, Anna L. Okorokova, Appelgren, Henrik, Tabish, Mohammad, Okorokov, Lev, Ekwall, Karl

Here we describe the phenotypic characterization of the cta4+ gene, encoding a novel member of the P4 family of P-type ATPases of fission yeast. The cta4Δ mutant is temperature sensitive and cold...

Specific functions for the fission yeast Sirtuins Hst2 and Hst4 in gene regulation and retrotransposon silencing

Durand-Dubief, Mickaël, Sinha, Indranil, Fagerström-Billai, Fredrik, Bonilla, Carolina, Wright, Anthony, Grunstein, Michael, ...

Expression profiling, ChiP–CHIP and phenotypic analysis were used to investigate the functional relationships of class III NAD+-dependent HDACs (Sirtuins) in fission yeast. We detected significant...

A genome-wide role for CHD remodelling factors and Nap1 in nucleosome disassembly

Walfridsson, Julian, Khorosjutina, Olga, Matikainen, Paulina, Gustafsson, Claes M, Ekwall, Karl

Chromatin remodelling factors and histone chaperones were previously shown to cooperatively affect nucleosome assembly and disassembly processes in vitro. Here, we show that Schizosaccharomyces pombe...

Cell-cycle regulation of cohesin stability along fission yeast chromosomes

Bernard, Pascal, Schmidt, Christine Katrin, Vaur, Sabine, Dheur, Sonia, Drogat, Julie, Genier, Sylvie, ...

Sister chromatid cohesion is mediated by cohesin, but the process of cohesion establishment during S-phase is still enigmatic. In mammalian cells, cohesin binding to chromatin is dynamic in G1, but...

The Schizosaccharomyces pombe JmjC-Protein, Msc1, Prevents H2A.Z Localization in Centromeric and Subtelomeric Chromatin Domains

Buchanan, Luke, Durand-Dubief, Mickaël, Roguev, Assen, Sakalar, Cagri, Wilhelm, Brian, Strålfors, Annelie, ...

Eukaryotic genomes are repetitively packaged into chromatin by nucleosomes, however they are regulated by the differences between nucleosomes, which establish various chromatin states. Local...