Karlheinz Altendorf

Membrane Topology Analysis of the Sensor Kinase KdpD of Escherichia coli* (2008)

Petra Zimmann, Karlheinz Altendorf

The expression of the kdpFABC operon, coding for the K �-translocating Kdp-ATPase, is under the control of the two regulatory proteins KdpD and KdpE, which belong to the group of sensor...

Lettuce for Human Consumption Collected in Costa Rica Contains Complex Communities of Culturable Oxytetracycline- and Gentamicin-Resistant Bacteria (2006)

César Rodríguez, Lore Lang, Amy Wang, Karlheinz Altendorf, O García, André Lipski

The present widespread use of antimicrobials in crop farming is based upon their successful application in human medicine. However, recent evidence suggests that the massive anthropogenic release of...

Community Analysis of Biofilters Using Fluorescence In Situ Hybridization Including a New Probe for the Xanthomonas Branch of the Class Proteobacteria

Friedrich, Udo, Naismith, Michèle M., Altendorf, Karlheinz, Lipski, André

Domain-, class-, and subclass-specific rRNA-targeted probes were applied to investigate the microbial communities of three industrial and three laboratory-scale biofilters. The set of probes also...

Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori: the Malate:Quinone Oxidoreductase

Kather, Birgit, Stingl, Kerstin, Van Der Rest, Michel E., Altendorf, Karlheinz, Molenaar, Douwe

The only enzyme of the citric acid cycle for which no open reading frame (ORF) was found in the Helicobacter pylori genome is the NAD-dependent malate dehydrogenase. Here, it is shown that in this...

Cs+ Induces the kdp Operon of Escherichia coli by Lowering the Intracellular K+ Concentration

Jung, Kirsten, Krabusch, Mechthild, Altendorf, Karlheinz

Cs+ was found to induce expression of the kdpFABC operon, encoding a high-affinity K+ uptake system of Escherichia coli. Quantitative expression analyses at the transcriptional and translational...

Prolonged Survival and Cytoplasmic pH Homeostasis of Helicobacter pylori at pH 1

Stingl, Kerstin, Uhlemann, Eva-Maria, Deckers-Hebestreit, Gabriele, Schmid, Roland, Bakker, Evert P., Altendorf, Karlheinz

In the presence of urea, Helicobacter pylori survived for at least 3 h at pH 1. Under these conditions, the cells maintained their cytoplasmic pH at 5.8. De novo protein synthesis during acid shock...

Energetics of Helicobacter pylori and Its Implications for the Mechanism of Urease-Dependent Acid Tolerance at pH 1

Stingl, Kerstin, Uhlemann, Eva-Maria, Schmid, Roland, Altendorf, Karlheinz, Bakker, Evert P.

In the presence of urea the neutrophilic human pathogen Helicobacter pylori survives for several hours at pH 1 with concomitant cytoplasmic pH homeostasis. To study this effect in detail, the...

Characterization of Amino Acid Substitutions in KdpA, the K+-Binding and -Translocating Subunit of the KdpFABC Complex of Escherichia coli

Van Der Laan, Martin, Gaßel, Michael, Altendorf, Karlheinz

When grown under K+ limitation, Escherichia coli induces the K+-translocating KdpFABC complex. The stimulation of ATPase activity by NH4+ ions was shown for the first time. Substitutions in KdpA,...

Physicochemical Cell Surface and Adhesive Properties of Coryneform Bacteria Related to the Presence and Chain Length of Mycolic Acids

Bendinger, Bernd, Rijnaarts, Huub H. M., Altendorf, Karlheinz, Zehnder, Alexander J. B.

The presence and chain length of mycolic acids of bacteria of the genera Corynebacterium, Rhodococcus, Gordona, Mycobacterium, and Arthrobacter and of coryneform bacteria containing a type B...

Differentiation of Gram-Negative, Nonfermentative Bacteria Isolated from Biofilters on the Basis of Fatty Acid Composition, Quinone System, and Physiological Reaction Profiles

Lipski, André, Klatte, Stefan, Bendinger, Bernd, Altendorf, Karlheinz

Gram-negative, nonfermentative bacteria isolated from biofilters for off-gas treatment of animal-rendering-plant emissions were differentiated by whole-cell fatty acid analysis, quinone analysis, and...

Role of an Electrical Potential in the Coupling of Metabolic Energy to Active Transport by Membrane Vesicles of Escherichia coli

Hirata, Hajime, Altendorf, Karlheinz, Harold, Franklin M.

Membrane vesicles from E. coli can oxidize D-lactate and other substrates and couple respiration to the active transport of sugars and amino acids. The present experiments bear on the nature of the...

Amino Acid Substitutions in Putative Selectivity Filter Regions III and IV in KdpA Alter Ion Selectivity of the KdpFABC Complex from Escherichia coli

Bertrand, Jessica, Altendorf, Karlheinz, Bramkamp, Marc

When grown under conditions of potassium limitation or high osmolality, Escherichia coli synthesizes the K+-translocating KdpFABC complex. The KdpA subunit, which has sequence homology to potassium...

An Atypical KdpD Homologue from the Cyanobacterium Anabaena sp. Strain L-31: Cloning, In Vivo Expression, and Interaction with Escherichia coli KdpD-CTD

Ballal, Anand, Bramkamp, Marc, Rajaram, Hema, Zimmann, Petra, Apte, Shree Kumar, Altendorf, Karlheinz

The kdpFABC operon of Escherichia coli, coding for the high-affinity K+ transport system KdpFABC, is transcriptionally regulated by the products of the adjacently located kdpDE genes. The KdpD...

Community Structure and Diversity of Biofilms from a Beer Bottling Plant as Revealed Using 16S rRNA Gene Clone Libraries†

Timke, Markus, Wang-Lieu, Ngoc Quynh, Altendorf, Karlheinz, Lipski, André

The microbial composition of biofilms from a beer bottling plant was analyzed by a cultivation independent analysis of the 16S rRNA genes. Clone libraries were differentiated by amplified 16S rRNA...

Community Analysis of Biofilters Using Fluorescence In Situ Hybridization Including a New Probe for the Xanthomonas Branch of the Class Proteobacteria

Friedrich, Udo, Naismith, Michèle M., Altendorf, Karlheinz, Lipski, André

Domain-, class-, and subclass-specific rRNA-targeted probes were applied to investigate the microbial communities of three industrial and three laboratory-scale biofilters. The set of probes also...

Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori: the Malate:Quinone Oxidoreductase

Kather, Birgit, Stingl, Kerstin, Van Der Rest, Michel E., Altendorf, Karlheinz, Molenaar, Douwe

The only enzyme of the citric acid cycle for which no open reading frame (ORF) was found in the Helicobacter pylori genome is the NAD-dependent malate dehydrogenase. Here, it is shown that in this...

Cs+ Induces the kdp Operon of Escherichia coli by Lowering the Intracellular K+ Concentration

Jung, Kirsten, Krabusch, Mechthild, Altendorf, Karlheinz

Cs+ was found to induce expression of the kdpFABC operon, encoding a high-affinity K+ uptake system of Escherichia coli. Quantitative expression analyses at the transcriptional and translational...

Prolonged Survival and Cytoplasmic pH Homeostasis of Helicobacter pylori at pH 1

Stingl, Kerstin, Uhlemann, Eva-Maria, Deckers-Hebestreit, Gabriele, Schmid, Roland, Bakker, Evert P., Altendorf, Karlheinz

In the presence of urea, Helicobacter pylori survived for at least 3 h at pH 1. Under these conditions, the cells maintained their cytoplasmic pH at 5.8. De novo protein synthesis during acid shock...

Energetics of Helicobacter pylori and Its Implications for the Mechanism of Urease-Dependent Acid Tolerance at pH 1

Stingl, Kerstin, Uhlemann, Eva-Maria, Schmid, Roland, Altendorf, Karlheinz, Bakker, Evert P.

In the presence of urea the neutrophilic human pathogen Helicobacter pylori survives for several hours at pH 1 with concomitant cytoplasmic pH homeostasis. To study this effect in detail, the...

Characterization of Amino Acid Substitutions in KdpA, the K+-Binding and -Translocating Subunit of the KdpFABC Complex of Escherichia coli

Van Der Laan, Martin, Gaßel, Michael, Altendorf, Karlheinz

When grown under K+ limitation, Escherichia coli induces the K+-translocating KdpFABC complex. The stimulation of ATPase activity by NH4+ ions was shown for the first time. Substitutions in KdpA,...

Physicochemical Cell Surface and Adhesive Properties of Coryneform Bacteria Related to the Presence and Chain Length of Mycolic Acids

Bendinger, Bernd, Rijnaarts, Huub H. M., Altendorf, Karlheinz, Zehnder, Alexander J. B.

The presence and chain length of mycolic acids of bacteria of the genera Corynebacterium, Rhodococcus, Gordona, Mycobacterium, and Arthrobacter and of coryneform bacteria containing a type B...

Differentiation of Gram-Negative, Nonfermentative Bacteria Isolated from Biofilters on the Basis of Fatty Acid Composition, Quinone System, and Physiological Reaction Profiles

Lipski, André, Klatte, Stefan, Bendinger, Bernd, Altendorf, Karlheinz

Gram-negative, nonfermentative bacteria isolated from biofilters for off-gas treatment of animal-rendering-plant emissions were differentiated by whole-cell fatty acid analysis, quinone analysis, and...

Role of an Electrical Potential in the Coupling of Metabolic Energy to Active Transport by Membrane Vesicles of Escherichia coli

Hirata, Hajime, Altendorf, Karlheinz, Harold, Franklin M.

Membrane vesicles from E. coli can oxidize D-lactate and other substrates and couple respiration to the active transport of sugars and amino acids. The present experiments bear on the nature of the...

Amino Acid Substitutions in Putative Selectivity Filter Regions III and IV in KdpA Alter Ion Selectivity of the KdpFABC Complex from Escherichia coli

Bertrand, Jessica, Altendorf, Karlheinz, Bramkamp, Marc

When grown under conditions of potassium limitation or high osmolality, Escherichia coli synthesizes the K+-translocating KdpFABC complex. The KdpA subunit, which has sequence homology to potassium...

An Atypical KdpD Homologue from the Cyanobacterium Anabaena sp. Strain L-31: Cloning, In Vivo Expression, and Interaction with Escherichia coli KdpD-CTD

Ballal, Anand, Bramkamp, Marc, Rajaram, Hema, Zimmann, Petra, Apte, Shree Kumar, Altendorf, Karlheinz

The kdpFABC operon of Escherichia coli, coding for the high-affinity K+ transport system KdpFABC, is transcriptionally regulated by the products of the adjacently located kdpDE genes. The KdpD...

Community Structure and Diversity of Biofilms from a Beer Bottling Plant as Revealed Using 16S rRNA Gene Clone Libraries†

Timke, Markus, Wang-Lieu, Ngoc Quynh, Altendorf, Karlheinz, Lipski, André

The microbial composition of biofilms from a beer bottling plant was analyzed by a cultivation independent analysis of the 16S rRNA genes. Clone libraries were differentiated by amplified 16S rRNA...

Lettuce for Human Consumption Collected in Costa Rica Contains Complex Communities of Culturable Oxytetracycline- and Gentamicin-Resistant Bacteria

Rodríguez, César, Lang, Lore, Wang, Amy, Altendorf, Karlheinz, García, Fernando, Lipski, André

The present widespread use of antimicrobials in crop farming is based upon their successful application in human medicine. However, recent evidence suggests that the massive anthropogenic release of...

Channel-mediated potassium uptake in Helicobacter pylori is essential for gastric colonization

Stingl, Kerstin, Brandt, Sonja, Uhlemann, Eva-Maria, Schmid, Roland, Altendorf, Karlheinz, Zeilinger, Carsten, ...

To date, the biological role of prokaryotic K+ channels remains unknown. Helicobacter pylori contains a gene encoding a putative K+ channel (HpKchA) of the two-transmembrane RCK (regulation of K+...

The Extension of the Fourth Transmembrane Helix of the Sensor Kinase KdpD of Escherichia coli Is Involved in Sensing▿

Zimmann, Petra, Steinbrügge, Anne, Schniederberend, Maren, Jung, Kirsten, Altendorf, Karlheinz

The KdpD sensor kinase and the KdpE response regulator control expression of the kdpFABC operon coding for the KdpFABC high-affinity K+ transport system of Escherichia coli. In search of a distinct...

Reduction of Turgor Is Not the Stimulus for the Sensor Kinase KdpD of Escherichia coli▿

Hamann, Knut, Zimmann, Petra, Altendorf, Karlheinz

Stimulus perception by the KdpD/KdpE two-component system of Escherichia coli is still controversial with respect to the nature of the stimulus that is perceived by the sensor kinase KdpD. Limiting...

Constant c10 Ring Stoichiometry in the Escherichia coli ATP Synthase Analyzed by Cross-Linking▿

Ballhausen, Britta, Altendorf, Karlheinz, Deckers-Hebestreit, Gabriele

The subunit c stoichiometry of Escherichia coli ATP synthase was studied by intermolecular cross-linking via oxidation of bi-cysteine-substituted subunit c (cA21C/cM65C). Independent of the carbon...