Katherine A. Borkovich

A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors (2006)

Colot, Hildur V, Park, Gyungsoon, Turner, Gloria E, Ringelberg, Carol, Crew, Christopher M, Litvinkova, Liubov, ...

The low rate of homologous recombination exhibited by wild-type strains of filamentous fungi has hindered development of high-throughput gene knockout procedures for this group of organisms. In this...

Hyphal development in Neurospora crassa: Involvement of a two-component histidine kinase (1996)

Alex, Lisa A., Borkovich, Katherine A., Simon, Melvin I.

Two-component signal transduction systems are most often found in prokaryotic organisms where they are responsible for mediating the cellular responses to many environmental stimuli. These systems...

Attenuation of Sensory Receptor Signaling by Covalent Modification (1992)

Borkovich, Katherine A., Alex, Lisa A., Simon, Melvin I.

The Tar receptor is a transmembrane protein that regulates bacterial chemotaxis in response to changes in the level of aspartic acid in the medium. The extracellular portion of the protein can bind...

Transmembrane Signal Transduction in Bacterial Chemotaxis Involves Ligand-Dependent Activation of Phosphate Group Transfer (1989)

Borkovich, Katherine A., Kaplan, Nachum, Hess, J. Fred, Simon, Melvin I.

Signal transduction in Escherichia coli involves the interaction of transmembrane receptor proteins such as the aspartate receptor, Tar, and the products of four chemotaxis genes, cheA, cheY, cheW,...

The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins

Bieszke, Jennifer A., Braun, Edward L., Bean, Laura E., Kang, Seogchan, Natvig, Donald O., Borkovich, Katherine A.

Opsins are a class of retinal-binding, seven transmembrane helix proteins that function as light-responsive ion pumps or sensory receptors. Previously, genes encoding opsins had been identified in...

Regulation of Conidiation and Adenylyl Cyclase Levels by the Gα Protein GNA-3 in Neurospora crassa

Kays, Ann M., Rowley, Patricia S., Baasiri, Rudeina A., Borkovich, Katherine A.

We have identified a new gene encoding the G protein α subunit, gna-3, from the filamentous fungus Neurospora crassa. The predicted amino acid sequence of GNA-3 is most similar to the Gα proteins...

Shared and Independent Roles for a Gαi Protein and Adenylyl Cyclase in Regulating Development and Stress Responses in Neurospora crassa

Ivey, F. Douglas, Kays, Ann M., Borkovich, Katherine A.

Growth and development are regulated using cyclic AMP (cAMP)-dependent and -independent pathways in Neurospora crassa. The cr-1 adenylyl cyclase mutant lacks detectable cAMP and exhibits numerous...

A G-Protein β Subunit Required for Sexual and Vegetative Development and Maintenance of Normal Gα Protein Levels in Neurospora crassa

Yang, Qi, Poole, Sheven I., Borkovich, Katherine A.

The genome of the filamentous fungus Neurospora crassa contains a single gene encoding a heterotrimeric G-protein β subunit, gnb-1. The predicted GNB-1 protein sequence is most identical to Gβ...

Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Borkovich, Katherine A., Alex, Lisa A., Yarden, Oded, Freitag, Michael, Turner, Gloria E., Read, Nick D., ...

We present an analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa. Seven major areas of Neurospora genomics and...

The Heterotrimeric G-Protein Subunits GNG-1 and GNB-1 Form a Gβγ Dimer Required for Normal Female Fertility, Asexual Development, and Gα Protein Levels in Neurospora crassa

Krystofova, Svetlana, Borkovich, Katherine A.

We have identified a gene encoding a heterotrimeric G protein γ subunit, gng-1, from the filamentous fungus Neurospora crassa. gng-1 possesses a gene structure similar to that of mammalian Gγ...

Pheromones Are Essential for Male Fertility and Sufficient To Direct Chemotropic Polarized Growth of Trichogynes during Mating in Neurospora crassa

Kim, Hyojeong, Borkovich, Katherine A.

Neurospora crassa is a self-sterile filamentous fungus with two mating types, mat A and mat a. Its mating involves chemotropic polarized growth of female-specific hyphae (trichogynes) toward male...

Severe impairment of growth and differentiation in a Neurospora crassa mutant lacking all heterotrimeric G alpha proteins.

Kays, Ann M, Borkovich, Katherine A

Heterotrimeric G alpha proteins play a critical role in regulating growth and differentiation in filamentous fungi. No systematic analysis of functional relationships between subunits has been...

GPR-4 Is a Predicted G-Protein-Coupled Receptor Required for Carbon Source-Dependent Asexual Growth and Development in Neurospora crassa

Li, Liande, Borkovich, Katherine A.

The filamentous fungus Neurospora crassa is able to utilize a wide variety of carbon sources. Here, we examine the involvement of a predicted G-protein-coupled receptor (GPCR), GPR-4, during growth...

The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins

Bieszke, Jennifer A., Braun, Edward L., Bean, Laura E., Kang, Seogchan, Natvig, Donald O., Borkovich, Katherine A.

Opsins are a class of retinal-binding, seven transmembrane helix proteins that function as light-responsive ion pumps or sensory receptors. Previously, genes encoding opsins had been identified in...

Regulation of Conidiation and Adenylyl Cyclase Levels by the Gα Protein GNA-3 in Neurospora crassa

Kays, Ann M., Rowley, Patricia S., Baasiri, Rudeina A., Borkovich, Katherine A.

We have identified a new gene encoding the G protein α subunit, gna-3, from the filamentous fungus Neurospora crassa. The predicted amino acid sequence of GNA-3 is most similar to the Gα proteins...

Shared and Independent Roles for a Gαi Protein and Adenylyl Cyclase in Regulating Development and Stress Responses in Neurospora crassa

Ivey, F. Douglas, Kays, Ann M., Borkovich, Katherine A.

Growth and development are regulated using cyclic AMP (cAMP)-dependent and -independent pathways in Neurospora crassa. The cr-1 adenylyl cyclase mutant lacks detectable cAMP and exhibits numerous...

A G-Protein β Subunit Required for Sexual and Vegetative Development and Maintenance of Normal Gα Protein Levels in Neurospora crassa

Yang, Qi, Poole, Sheven I., Borkovich, Katherine A.

The genome of the filamentous fungus Neurospora crassa contains a single gene encoding a heterotrimeric G-protein β subunit, gnb-1. The predicted GNB-1 protein sequence is most identical to Gβ...

Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Borkovich, Katherine A., Alex, Lisa A., Yarden, Oded, Freitag, Michael, Turner, Gloria E., Read, Nick D., ...

We present an analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa. Seven major areas of Neurospora genomics and...

The Heterotrimeric G-Protein Subunits GNG-1 and GNB-1 Form a Gβγ Dimer Required for Normal Female Fertility, Asexual Development, and Gα Protein Levels in Neurospora crassa

Krystofova, Svetlana, Borkovich, Katherine A.

We have identified a gene encoding a heterotrimeric G protein γ subunit, gng-1, from the filamentous fungus Neurospora crassa. gng-1 possesses a gene structure similar to that of mammalian Gγ...

Pheromones Are Essential for Male Fertility and Sufficient To Direct Chemotropic Polarized Growth of Trichogynes during Mating in Neurospora crassa

Kim, Hyojeong, Borkovich, Katherine A.

Neurospora crassa is a self-sterile filamentous fungus with two mating types, mat A and mat a. Its mating involves chemotropic polarized growth of female-specific hyphae (trichogynes) toward male...

Severe impairment of growth and differentiation in a Neurospora crassa mutant lacking all heterotrimeric G alpha proteins.

Kays, Ann M, Borkovich, Katherine A

Heterotrimeric G alpha proteins play a critical role in regulating growth and differentiation in filamentous fungi. No systematic analysis of functional relationships between subunits has been...

GPR-4 Is a Predicted G-Protein-Coupled Receptor Required for Carbon Source-Dependent Asexual Growth and Development in Neurospora crassa

Li, Liande, Borkovich, Katherine A.

The filamentous fungus Neurospora crassa is able to utilize a wide variety of carbon sources. Here, we examine the involvement of a predicted G-protein-coupled receptor (GPCR), GPR-4, during growth...

The Predicted G-Protein-Coupled Receptor GPR-1 Is Required for Female Sexual Development in the Multicellular Fungus Neurospora crassa

Krystofova, Svetlana, Borkovich, Katherine A.

G-protein-coupled receptors (GPCRs) control important aspects of asexual and sexual development in eukaryotic organisms. We have identified a predicted GPCR in the filamentous fungus Neurospora...

A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors

Colot, Hildur V., Park, Gyungsoon, Turner, Gloria E., Ringelberg, Carol, Crew, Christopher M., Litvinkova, Liubov, ...

The low rate of homologous recombination exhibited by wild-type strains of filamentous fungi has hindered development of high-throughput gene knockout procedures for this group of organisms. In this...

The Response Regulator RRG-1 Functions Upstream of a Mitogen-activated Protein Kinase Pathway Impacting Asexual Development, Female Fertility, Osmotic Stress, and Fungicide Resistance in Neurospora crassa

Jones, Carol A., Greer-Phillips, Suzanne E., Borkovich, Katherine A.

Two-component systems, consisting of proteins with histidine kinase and/or response regulator domains, regulate environmental responses in bacteria, Archaea, fungi, slime molds, and plants. Here, we...

Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPK

Vitalini, Michael W., De Paula, Renato M., Goldsmith, Charles S., Jones, Carol A., Borkovich, Katherine A., Bell-Pedersen, Deborah

Circadian clocks are composed of central oscillators, input pathways that transduce external information to the oscillators, and output pathways that allow the oscillators to temporally regulate...

Mitogen-Activated Protein Kinase Cascade Required for Regulation of Development and Secondary Metabolism in Neurospora crassa▿

Park, Gyungsoon, Pan, Songqin, Borkovich, Katherine A.

Mitogen-activated protein kinase (MAPK) signaling cascades are composed of MAPK kinase kinases (MAPKKKs), MAPK kinases (MAPKKs), and MAPKs. In this study, we characterize components of a MAPK cascade...