Kathleen Mihalik

Long-Term Follow-Up of Chimpanzees Inoculated with the First Infectious Clone for Hepatitis C Virus

Major, Marian E., Mihalik, Kathleen, Fernandez, Javier, Seidman, Jessica, Kleiner, David, Kolykhalov, Alexander A., ...

Two chimpanzees (Ch1535 and Ch1536) became infected with hepatitis C virus (HCV) following intrahepatic inoculation with RNA transcribed from a full-length cDNA clone of the virus. Both animals were...

Molecular and Immunological Significance of Chimpanzee Major Histocompatibility Complex Haplotypes for Hepatitis C Virus Immune Response and Vaccination Studies

Mizukoshi, Eishiro, Nascimbeni, Michelina, Blaustein, Joshua B., Mihalik, Kathleen, Rice, Charles M., Liang, T. Jake, ...

The chimpanzee is a critical animal model for studying cellular immune responses to infectious pathogens such as hepatitis B and C viruses, human immunodeficiency virus, and malaria. Several...

Previously Infected and Recovered Chimpanzees Exhibit Rapid Responses That Control Hepatitis C Virus Replication upon Rechallenge

Major, Marian E., Mihalik, Kathleen, Puig, Montserrat, Rehermann, Barbara, Nascimbeni, Michelina, Rice, Charles M., ...

Responses in three chimpanzees were compared following challenge with a clonal hepatitis C virus (HCV) contained in plasma from an animal that had received infectious RNA transcripts. Two of the...

Kinetics of CD4+ and CD8+ Memory T-Cell Responses during Hepatitis C Virus Rechallenge of Previously Recovered Chimpanzees

Nascimbeni, Michelina, Mizukoshi, Eishiro, Bosmann, Markus, Major, Marian E., Mihalik, Kathleen, Rice, Charles M., ...

The immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood. Antibodies to HCV structural proteins do not appear to play a key role in clearance of the virus and...

Long-Term Persistence of Infection in Chimpanzees Inoculated with an Infectious Hepatitis C Virus Clone Is Associated with a Decrease in the Viral Amino Acid Substitution Rate and Low Levels of Heterogeneity

Fernandez, Javier, Taylor, Deborah, Morhardt, Duncan R., Mihalik, Kathleen, Puig, Montserrat, Rice, Charles M., ...

Two chimpanzees, 1535 and 1536, became persistently infected following inoculation with RNA transcripts from cDNA clones of hepatitis C virus (HCV). Analysis of the HCV genomes from both animals...

New Antiviral Pathway That Mediates Hepatitis C Virus Replicon Interferon Sensitivity through ADAR1

Taylor, Deborah R., Puig, Montserrat, Darnell, Miriam E. R., Mihalik, Kathleen, Feinstone, Stephen M.

While many clinical hepatitis C virus (HCV) infections are resistant to alpha interferon (IFN-α) therapy, subgenomic in vitro self-replicating HCV RNAs (HCV replicons) are characterized by marked...

Long-Term Follow-Up of Chimpanzees Inoculated with the First Infectious Clone for Hepatitis C Virus

Major, Marian E., Mihalik, Kathleen, Fernandez, Javier, Seidman, Jessica, Kleiner, David, Kolykhalov, Alexander A., ...

Two chimpanzees (Ch1535 and Ch1536) became infected with hepatitis C virus (HCV) following intrahepatic inoculation with RNA transcribed from a full-length cDNA clone of the virus. Both animals were...

Molecular and Immunological Significance of Chimpanzee Major Histocompatibility Complex Haplotypes for Hepatitis C Virus Immune Response and Vaccination Studies

Mizukoshi, Eishiro, Nascimbeni, Michelina, Blaustein, Joshua B., Mihalik, Kathleen, Rice, Charles M., Liang, T. Jake, ...

The chimpanzee is a critical animal model for studying cellular immune responses to infectious pathogens such as hepatitis B and C viruses, human immunodeficiency virus, and malaria. Several...

Previously Infected and Recovered Chimpanzees Exhibit Rapid Responses That Control Hepatitis C Virus Replication upon Rechallenge

Major, Marian E., Mihalik, Kathleen, Puig, Montserrat, Rehermann, Barbara, Nascimbeni, Michelina, Rice, Charles M., ...

Responses in three chimpanzees were compared following challenge with a clonal hepatitis C virus (HCV) contained in plasma from an animal that had received infectious RNA transcripts. Two of the...

Kinetics of CD4+ and CD8+ Memory T-Cell Responses during Hepatitis C Virus Rechallenge of Previously Recovered Chimpanzees

Nascimbeni, Michelina, Mizukoshi, Eishiro, Bosmann, Markus, Major, Marian E., Mihalik, Kathleen, Rice, Charles M., ...

The immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood. Antibodies to HCV structural proteins do not appear to play a key role in clearance of the virus and...

Long-Term Persistence of Infection in Chimpanzees Inoculated with an Infectious Hepatitis C Virus Clone Is Associated with a Decrease in the Viral Amino Acid Substitution Rate and Low Levels of Heterogeneity

Fernandez, Javier, Taylor, Deborah, Morhardt, Duncan R., Mihalik, Kathleen, Puig, Montserrat, Rice, Charles M., ...

Two chimpanzees, 1535 and 1536, became persistently infected following inoculation with RNA transcripts from cDNA clones of hepatitis C virus (HCV). Analysis of the HCV genomes from both animals...

New Antiviral Pathway That Mediates Hepatitis C Virus Replicon Interferon Sensitivity through ADAR1

Taylor, Deborah R., Puig, Montserrat, Darnell, Miriam E. R., Mihalik, Kathleen, Feinstone, Stephen M.

While many clinical hepatitis C virus (HCV) infections are resistant to alpha interferon (IFN-α) therapy, subgenomic in vitro self-replicating HCV RNAs (HCV replicons) are characterized by marked...

Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C

Manigold, Tobias, Shin, Eui-Cheol, Mizukoshi, Eishiro, Mihalik, Kathleen, Murthy, Krishna K., Rice, Charles M., ...

Hepatitis C virus (HCV) poses a global health problem because it readily establishes persistent infection and a vaccine is not available. CD4+CD25+ T cells have been implicated in HCV persistence...

Evidence for a functional RNA element in the hepatitis C virus core gene

McMullan, Laura K., Grakoui, Arash, Evans, Matthew J., Mihalik, Kathleen, Puig, Montserrat, Branch, Andrea D., ...

In the core protein-coding region of hepatitis C virus (HCV), evidence exists for both phylogenetically conserved RNA structures and a +1 alternative reading frame (ARF). To investigate its role in...

Hepatitis C virus epitope-specific neutralizing antibodies in Igs prepared from human plasma

Zhang, Pei, Wu, Charles G., Mihalik, Kathleen, Virata-Theimer, Maria Luisa, Yu, Mei-ying W., Alter, Harvey J., ...

Neutralizing antibodies directed against hepatitis C virus (HCV) are present in Igs made from anti-HCV-positive plasma. However, these HCV-specific Igs are largely ineffective in vivo. The mechanism...

Depletion of interfering antibodies in chronic hepatitis C patients and vaccinated chimpanzees reveals broad cross-genotype neutralizing activity

Zhang, Pei, Zhong, Lilin, Struble, Evi Budo, Watanabe, Hisayoshi, Kachko, Alla, Mihalik, Kathleen, ...

Using human immune globulins made from antihepatitis C virus (HCV)-positive plasma, we recently identified two antibody epitopes in the E2 protein at residues 412–426 (epitope I) and 434–446...