Golgi structure in three dimensions: functional insights from the normal rat kidney cell (1999)
Mark S. Ladinsky, David N. Mastronarde, J. Richard Mcintosh, Kathryn E. Howell, L. Andrew Staehelin
Abstract. Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at...
Taylor, Randall S., Jones, Steven M., Dahl, Rolf H., Nordeen, Mark H., Howell, Kathryn E.
To characterize endogenous molecules and activities of the Golgi complex, proteins in transit were >99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment. The loss of proteins in...
Marsh, Brad J., Mastronarde, David N., Buttle, Karolyn F., Howell, Kathryn E., McIntosh, J. Richard
The positional relationships among all of the visible organelles in a densely packed region of cytoplasm from an insulin secreting, cultured mammalian cell have been analyzed in three dimensions...
Ladinsky, Mark S., Wu, Christine C., McIntosh, Shane, McIntosh, J. Richard, Howell, Kathryn E.
Incubating cells at 20°C blocks transport out of the Golgi complex and amplifies the exit compartments. We have used the 20°C block, followed by EM tomography and serial section reconstruction, to...
Marsh, Brad J., Volkmann, Niels, McIntosh, J. Richard, Howell, Kathryn E.
Direct continuity between the membranes of cisternae in the Golgi complex in mammalian cells rarely has been observed; when seen, its documentation has been equivocal. Here we have used dual-axis...
Organellar Proteomics Reveals Golgi Arginine DimethylationD⃞
Wu, Christine C., MacCoss, Michael J., Mardones, Gonzalo, Finnigan, Claire, Mogelsvang, Soren, Yates, John R., ...
The Golgi complex functions to posttranslationally modify newly synthesized proteins and lipids and to sort them to their sites of function. In this study, a stacked Golgi fraction was isolated by...
A large-conductance anion channel of the Golgi complex.
Thompson, Roger J, Nordeen, Mark H, Howell, Kathryn E, Caldwell, John H
An acidic lumenal pH is vital for the proper posttranslational modifications and sorting of proteins and lipids from the Golgi complex. We characterized ion channels present in Golgi fractions that...
Snyder, Christopher M., Mardones, Gonzalo A., Ladinsky, Mark S., Howell, Kathryn E.
The trans-Golgi matrix consists of a group of proteins dynamically associated with the trans-Golgi and thought to be involved in anterograde and retrograde Golgi traffic, as well as interactions with...
Mardones, Gonzalo A., Snyder, Christopher M., Howell, Kathryn E.
The role of cis-medial Golgi matrix proteins in retrograde traffic is poorly understood. We have used imaging techniques to understand the relationship between the cis-medial Golgi matrix and...
Taylor, Randall S., Jones, Steven M., Dahl, Rolf H., Nordeen, Mark H., Howell, Kathryn E.
To characterize endogenous molecules and activities of the Golgi complex, proteins in transit were >99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment. The loss of proteins in...
Marsh, Brad J., Mastronarde, David N., Buttle, Karolyn F., Howell, Kathryn E., McIntosh, J. Richard
The positional relationships among all of the visible organelles in a densely packed region of cytoplasm from an insulin secreting, cultured mammalian cell have been analyzed in three dimensions...
Ladinsky, Mark S., Wu, Christine C., McIntosh, Shane, McIntosh, J. Richard, Howell, Kathryn E.
Incubating cells at 20°C blocks transport out of the Golgi complex and amplifies the exit compartments. We have used the 20°C block, followed by EM tomography and serial section reconstruction, to...
Marsh, Brad J., Volkmann, Niels, McIntosh, J. Richard, Howell, Kathryn E.
Direct continuity between the membranes of cisternae in the Golgi complex in mammalian cells rarely has been observed; when seen, its documentation has been equivocal. Here we have used dual-axis...
Organellar Proteomics Reveals Golgi Arginine DimethylationD⃞
Wu, Christine C., MacCoss, Michael J., Mardones, Gonzalo, Finnigan, Claire, Mogelsvang, Soren, Yates, John R., ...
The Golgi complex functions to posttranslationally modify newly synthesized proteins and lipids and to sort them to their sites of function. In this study, a stacked Golgi fraction was isolated by...
A large-conductance anion channel of the Golgi complex.
Thompson, Roger J, Nordeen, Mark H, Howell, Kathryn E, Caldwell, John H
An acidic lumenal pH is vital for the proper posttranslational modifications and sorting of proteins and lipids from the Golgi complex. We characterized ion channels present in Golgi fractions that...
Snyder, Christopher M., Mardones, Gonzalo A., Ladinsky, Mark S., Howell, Kathryn E.
The trans-Golgi matrix consists of a group of proteins dynamically associated with the trans-Golgi and thought to be involved in anterograde and retrograde Golgi traffic, as well as interactions with...
Mardones, Gonzalo A., Snyder, Christopher M., Howell, Kathryn E.
The role of cis-medial Golgi matrix proteins in retrograde traffic is poorly understood. We have used imaging techniques to understand the relationship between the cis-medial Golgi matrix and...
Jones, Steven M., Howell, Kathryn E.
An 85-kD cytosolic complex (p62cplx), consisting of a 62-kD phosphoprotein (p62) and a 25-kD GTPase, has been shown to be essential for the cell-free reconstitution of polymeric IgA receptor...
Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell
Ladinsky, Mark S., Mastronarde, David N., McIntosh, J. Richard, Howell, Kathryn E., Staehelin, L. Andrew
Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at ∼7-nm...