A ubiquitin ligase HRD1 promotes the degradation of Pael receptor, a substrate of Parkin. (2006)
Omura, Tomohiro, Kaneko, Masayuki, Okuma, Yasunobu, Orba, Yasuko, Nagashima, Kazuo, Takahashi, Ryosuke, ...
It has been proposed that in autosomal recessive juvenile parkinsonism (AR-JP), a ubiquitin ligase (E3) Parkin, which is involved in endoplasmic reticulum-associated degradation (ERAD), lacks E3...
A ubiquitin ligase HRD1 promotes the degradation of Pael receptor, a substrate of Parkin. (2006)
Omura, Tomohiro, Kaneko, Masayuki, Okuma, Yasunobu, Orba, Yasuko, Nagashima, Kazuo, Takahashi, Ryosuke, ...
It has been proposed that in autosomal recessive juvenile parkinsonism (AR-JP), a ubiquitin ligase (E3) Parkin, which is involved in endoplasmic reticulum-associated degradation (ERAD), lacks E3...
Nakamura, Koichiro, Jeong, Seon-Yong, Uchihara, Toshiki, Anno, Midori, Nagashima, Kazuo, Nagashima, Toshiko, ...
Genetic etiologies of at least 20% of autosomal dominant cerebellar ataxias (ADCAs) have yet to be clarified. We identified a novel spinocerebellar ataxia (SCA) form in four Japanese pedigrees...
Komori, Masayuki, Hashizume, Takanori, Ohi, Hiroaki, Miura, Toshiaki, Kitada, Mitsukazu, Nagashima, Kazuo, ...
Three forms of cytochrome P-450, designated as P-450-HM1, P-450-HM2, and P-450-HM3, were isolated from human liver microsomes using high-performance liquid chromntography (HPLC) techniques. Each...
EBATO, Hisamoto, ABE, Toshiaki, YAMAKAWA, Tamio, NAGASHIMA, Kazuo
The twisted tubular cytoplasmic inclusion bodies were isolated from spleens of four patients with adult form Gaucher′s disease. The chemical composition of the CIB (cytoplasmic inclusion bodies)...
Nagai, Makoto, Tanaka, Shinya, Tsuda, Masumi, Endo, Shuichi, Kato, Hiroyuki, Sonobe, Hiroshi, ...
Human synovial sarcoma has been shown to exclusively harbor the chromosomal translocation t(X;18) that produces the chimeric gene SYT-SSX. However, the role of SYT-SSX in cellular transformation...
Rap2 as a Slowly Responding Molecular Switch in the Rap1 Signaling Cascade
Ohba, Yusuke, Mochizuki, Naoki, Matsuo, Keiko, Yamashita, Shigeko, Nakaya, Mie, Hashimoto, Yuko, ...
Rap2 is a member of the Ras family of GTPases and exhibits 60% identity to Rap1, but the function and regulation of Rap2 remain obscure. We found that, unlike the other Ras family proteins, the...
Takahashi, Hidehiro, Iwata, Toshinari, Kitagawa, Yoshinori, Takahashi, Reisuke H., Sato, Yuko, Wakabayashi, Hideki, ...
We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that ɛ and γ isoforms were specifically increased in premortem...
Shishido-Hara, Yukiko, Hara, Yoshinobu, Larson, Theresa, Yasui, Kotaro, Nagashima, Kazuo, Stoner, Gerald L.
Human polyomavirus JC (JCV) can encode the three capsid proteins VP1, VP2, and VP3, downstream of the agnoprotein in the late region. JCV virions are identified in the nucleus of infected cells. In...
Safak, Mahmut, Barrucco, Robert, Darbinyan, Armine, Okada, Yuki, Nagashima, Kazuo, Khalili, Kamel
In addition to encoding the structural and regulatory proteins, many viruses encode auxiliary proteins, some of which have been shown to play important roles in lytic and latent states of the...
Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis
Ohba, Yusuke, Ikuta, Koichi, Ogura, Atsuo, Matsuda, Junichiro, Mochizuki, Naoki, Nagashima, Kazuo, ...
C3G is a guanine nucleotide exchange factor (GEF) for Rap1, and is activated via Crk adaptor protein. To understand the physiological role of C3G, we generated C3G knockout mice. C3G–/–...
Chk2-deficient mice exhibit radioresistance and defective p53-mediated transcription
Takai, Hiroyuki, Naka, Kazuhito, Okada, Yuki, Watanabe, Miho, Harada, Naoki, Saito, Shin’ichi, ...
The mammalian Chk2 kinase is thought to mediate ATM-dependent signaling in response to DNA damage. The physiological role of mammalian Chk2 has now been investigated by the generation of...
Kobayashi, Yosuke, Watanabe, Miho, Okada, Yuki, Sawa, Hirofumi, Takai, Hiroyuki, Nakanishi, Makoto, ...
A growing number of DNA polymerases have been identified, although their physiological function and relation to human disease remain mostly unknown. DNA polymerase λ (Pol λ; also known as Pol β2)...
Oligosaccharides as Receptors for JC Virus
Komagome, Rika, Sawa, Hirofumi, Suzuki, Takashi, Suzuki, Yasuo, Tanaka, Shinya, Atwood, Walter J., ...
JC virus (JCV) belongs to the polyomavirus family of double-stranded DNA viruses and in humans causes a demyelinating disease of the central nervous system, progressive multifocal...
Inhibition of Virus Production in JC Virus-Infected Cells by Postinfection RNA Interference
Orba, Yasuko, Sawa, Hirofumi, Iwata, Hiroshi, Tanaka, Shinya, Nagashima, Kazuo
RNA interference has been applied for the prevention of virus infections in mammalian cells but has not succeeded in eliminating infections from already infected cells. We now show that the...
Epstein-Barr Virus Transforming Protein LMP1 Plays a Critical Role in Virus Production
Ahsan, Nazmul, Kanda, Teru, Nagashima, Kazuo, Takada, Kenzo
The Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1), which is critical for EBV-induced B-cell transformation, is also abundantly expressed during the lytic cycle of viral replication....
Okada, Yuki, Suzuki, Tadaki, Sunden, Yuji, Orba, Yasuko, Kose, Shingo, Imamoto, Naoko, ...
The nuclear envelope is one of the chief obstacles to the translocation of macromolecules that are larger than the diameter of nuclear pores. Heterochromatin protein 1 (HP1) bound to the lamin B...
Nagai, Makoto, Tanaka, Shinya, Tsuda, Masumi, Endo, Shuichi, Kato, Hiroyuki, Sonobe, Hiroshi, ...
Human synovial sarcoma has been shown to exclusively harbor the chromosomal translocation t(X;18) that produces the chimeric gene SYT-SSX. However, the role of SYT-SSX in cellular transformation...
Rap2 as a Slowly Responding Molecular Switch in the Rap1 Signaling Cascade
Ohba, Yusuke, Mochizuki, Naoki, Matsuo, Keiko, Yamashita, Shigeko, Nakaya, Mie, Hashimoto, Yuko, ...
Rap2 is a member of the Ras family of GTPases and exhibits 60% identity to Rap1, but the function and regulation of Rap2 remain obscure. We found that, unlike the other Ras family proteins, the...
Takahashi, Hidehiro, Iwata, Toshinari, Kitagawa, Yoshinori, Takahashi, Reisuke H., Sato, Yuko, Wakabayashi, Hideki, ...
We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that ɛ and γ isoforms were specifically increased in premortem...
Shishido-Hara, Yukiko, Hara, Yoshinobu, Larson, Theresa, Yasui, Kotaro, Nagashima, Kazuo, Stoner, Gerald L.
Human polyomavirus JC (JCV) can encode the three capsid proteins VP1, VP2, and VP3, downstream of the agnoprotein in the late region. JCV virions are identified in the nucleus of infected cells. In...
Safak, Mahmut, Barrucco, Robert, Darbinyan, Armine, Okada, Yuki, Nagashima, Kazuo, Khalili, Kamel
In addition to encoding the structural and regulatory proteins, many viruses encode auxiliary proteins, some of which have been shown to play important roles in lytic and latent states of the...
Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis
Ohba, Yusuke, Ikuta, Koichi, Ogura, Atsuo, Matsuda, Junichiro, Mochizuki, Naoki, Nagashima, Kazuo, ...
C3G is a guanine nucleotide exchange factor (GEF) for Rap1, and is activated via Crk adaptor protein. To understand the physiological role of C3G, we generated C3G knockout mice. C3G–/–...
Chk2-deficient mice exhibit radioresistance and defective p53-mediated transcription
Takai, Hiroyuki, Naka, Kazuhito, Okada, Yuki, Watanabe, Miho, Harada, Naoki, Saito, Shin’ichi, ...
The mammalian Chk2 kinase is thought to mediate ATM-dependent signaling in response to DNA damage. The physiological role of mammalian Chk2 has now been investigated by the generation of...
Kobayashi, Yosuke, Watanabe, Miho, Okada, Yuki, Sawa, Hirofumi, Takai, Hiroyuki, Nakanishi, Makoto, ...
A growing number of DNA polymerases have been identified, although their physiological function and relation to human disease remain mostly unknown. DNA polymerase λ (Pol λ; also known as Pol β2)...
Oligosaccharides as Receptors for JC Virus
Komagome, Rika, Sawa, Hirofumi, Suzuki, Takashi, Suzuki, Yasuo, Tanaka, Shinya, Atwood, Walter J., ...
JC virus (JCV) belongs to the polyomavirus family of double-stranded DNA viruses and in humans causes a demyelinating disease of the central nervous system, progressive multifocal...
Inhibition of Virus Production in JC Virus-Infected Cells by Postinfection RNA Interference
Orba, Yasuko, Sawa, Hirofumi, Iwata, Hiroshi, Tanaka, Shinya, Nagashima, Kazuo
RNA interference has been applied for the prevention of virus infections in mammalian cells but has not succeeded in eliminating infections from already infected cells. We now show that the...
Epstein-Barr Virus Transforming Protein LMP1 Plays a Critical Role in Virus Production
Ahsan, Nazmul, Kanda, Teru, Nagashima, Kazuo, Takada, Kenzo
The Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1), which is critical for EBV-induced B-cell transformation, is also abundantly expressed during the lytic cycle of viral replication....
Okada, Yuki, Suzuki, Tadaki, Sunden, Yuji, Orba, Yasuko, Kose, Shingo, Imamoto, Naoko, ...
The nuclear envelope is one of the chief obstacles to the translocation of macromolecules that are larger than the diameter of nuclear pores. Heterochromatin protein 1 (HP1) bound to the lamin B...
Miyasaka, Tomohiro, Morishima-Kawashima, Maho, Ravid, Rivka, Heutink, Peter, Van Swieten, John C., Nagashima, Kazuo, ...
Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) is a familial neurological disorder, characterized genetically by autosomal dominant inheritance, clinically by behavioral...
R-Ras Regulates Exocytosis by Rgl2/Rlf-mediated Activation of RalA on Endosomes
Takaya, Akiyuki, Kamio, Takahiro, Masuda, Michitaka, Mochizuki, Naoki, Sawa, Hirofumi, Sato, Mami, ...
R-Ras is a Ras-family small GTPase that regulates various cellular functions such as apoptosis and cell adhesion. Here, we demonstrate a role of R-Ras in exocytosis. By the use of specific anti-R-Ras...
Activity of Rho-family GTPases during cell division as visualized with FRET-based probes
Yoshizaki, Hisayoshi, Ohba, Yusuke, Kurokawa, Kazuo, Itoh, Reina E., Nakamura, Takeshi, Mochizuki, Naoki, ...
Rho-family GTPases regulate many cellular functions. To visualize the activity of Rho-family GTPases in living cells, we developed fluorescence resonance energy transfer (FRET)–based probes for...