Keiko Kobayashi

Complete blockage of the mevalonate pathway results in male gametophyte lethality (2009)

Suzuki, Masashi, Nakagawa, Shoko, Kamide, Yukiko, Kobayashi, Keiko, Ohyama, Kiyoshi, Hashinokuchi, Hiromi, ...

Plants have two isoprenoid biosynthetic pathways: the cytosolic mevalonate (MVA) pathway and the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Since the discovery of the MEP pathway,...

Identification of the Imprinted KLF14 Transcription Factor Undergoing Human-Specific Accelerated Evolution (2007)

Layla Parker-Katiraee, Andrew R. Carson, Takahiro Yamada, Philippe Arnaud, Robert Feil, ...

Imprinted genes are expressed in a parent-of-origin manner and are located in clusters throughout the genome. Aberrations in the expression of imprinted genes on human Chromosome 7 have been...

Identification of the Imprinted KLF14 Transcription Factor Undergoing Human-Specific Accelerated Evolution (2007)

Layla Parker-Katiraee, Andrew R Carson, Takahiro Yamada, Philippe Arnaud, Robert Feil, ...

Imprinted genes are expressed in a parent-of-origin manner and are located in clusters throughout the genome. Aberrations in the expression of imprinted genes on human chromosome 7 have been...

LOVASTATIN INSENSITIVE 1, a Novel Pentatricopeptide Repeat Protein, is a Potential Regulatory Factor of Isoprenoid Biosynthesis in Arabidopsis (2007)

Kobayashi, Keiko, Suzuki, Masashi, Tang, Jianwei, Nagata, Noriko, Ohyama, Kiyoshi, Seki, Hikaru, ...

Higher plants have two metabolic pathways for isoprenoid biosynthesis: the cytosolic mevalonate (MVA) pathway and the plastidal non-mevalonate (MEP) pathway. Despite the compartmentalization of these...

Caldecrin Is a Novel-Type Serine Protease Expressed in Pancreas, but Its Homologue, Elastase IV, Is an Artifact during Cloning Derived from Caldecrin Gene (1998)

Yoshino-Yasuda, Izumi, Kobayashi, Keiko, Akiyama, Masashi, Itoh, Hirotaka, Tomomura, Akito, Saheki, Takeyori

As reported previously, caldecrin, a serum calcium-decreasing factor, from pancreas was found to be a serine protease, but the proteolytic activity was not necessary for its serum calcium decreasing...

Individual Expression of Candida tropicalis Peroxisomal and Mitochondrial Carnitine Acetyltransferase-Encoding Genes and Subcellular Localization of the Products in Saccharomyces cerevisiae (1996)

Kawachi, Hiroyuki, Atomi, Haruyuki, Ueda, Mitsuyoshi, Hashimoto, Noriko, Kobayashi, Keiko, Yoshida, Tomoko, ...

In an n-alkane-assimilating yeast, Candida tropicalis, carnitine acetyltransferase (CAT; EC 2.3.1.7) was localized in both peroxisomes and mitochondria. Both CATs were encoded by one gene, CT-CAT,...

Purification and Characterization of Thrombopoietin (1995)

Kato, Takashi, Ogami, Kinya, Shimada, Yoshihiro, Iwamatsu, Akihiro, Sohma, Yoshiaki, Akahori, Hiromichi, ...

A thrombopoietic factor, termed thrombopoietin (TPO), was highly purified directly from the plasma of sublethally irradiated 1, 100 rats by measuring the production of megakaryo-cytes from a highly...

Amino Acid Sequence of Rat Argininosuccinate Lyase Deduced from cDNA (1988)

Amaya, Yoshihiro, Matsubasa, Tadashi, Takiguchi, Masaki, Kobayashi, Keiko, Saheki, Takeyori, Kawamoto, Susumu, ...

Argininosuccinate lyase [EC 4.3.2.1] is an enzyme of the urea cycle in the liver of ureotelic animals. The enzymes of the urea cycle, including argininosuccinate lyase, are regulated developmentally...

End Group Analysis of the Cytosolic and Mitochondrial Fumarases from Rat Liver (1983)

KOBAYASHI, Keiko, TUBOI, Syozo

Some molecular properties of the cytosolic and mitochondrial fumarases were compared. The carboxyl(C)-terminal amino acid of both the cytosolic and mitochondrial fumarases of rat liver cell was...

Studies on the Turnover Rates of Cytosolic and Mitochondrial Fumarases in Rat Liver (1982)

KOBAYASHI, Keiko, KAMIMURA, Tadashi, TUBOI, Syozo

The turnover rates of mitochondrial and cytosolic fumarase in the rat liver were determined by injecting L-[U-14C]leucine and following the decay of specific radioactivity incorporated into...

Physicochemical, Catalytic, and Immunochemical Properties of Fumarases Crystallized Separately from Mitochondrial and Cytosolic Fractions of Rat Liver (1981)

KOBAYASHI, Keiko, YAMANISHI, Tomoko, TUBOI, Syozo

Fumarases located in the cytosolic and mitochondrial fractions were separately purified and crystallized from rat liver. Polyacrylamide gel electrophoresis of these two crystallized fumarases in the...

Selective Cleavage of Peptide Bonds by a Serine Protease from the Muscle Layer of Rat Small Intestine (1978)

KOBAYASHI, Keiko, KATUNUMA, Nobuhiko

The kinetic constants of a serine protease from the muscle layer of rat small intestine for three ester substrates were compared with those reported for bovine chymotrypsin A. The Km values for...

Selective Cleavage of Peptide Bonds by a Serine Protease from Rat Skeletal Muscle (1978)

KOBAYASHI, Keiko, SANADA, Yukihiro, KATUNUMA, Nobuhiko

The selective cleavage of peptide bonds by a serine protease from skeletal muscle (SK-protease) was examined using glucagon and neurotensin as substrates. Among the peptide bonds cleaved in these...

Use of Rabbit Antibody IgG Bound onto Plain and Aminoalkylsilyl Glass Surface for the Enzyme-Linked Sandwich Immunoassay (1977)

KATO, Kanefusa, HAMAGUCHI, Yoshitaka, OKAWA, Sachiyo, ISHIKAWA, Eiji, KOBAYASHI, Keiko, KATUNUMA, Nobuhiko

Rabbit antibody IgG was bound onto aminoalkylsilyl or plain glass rods by simple adsorption. For comparison, rabbit antibody IgG was also bound onto glutaraldehyde-activated aminoalkylsilyl glass...

Use of Rabbit Antibody IgG-loaded Silicone Pieces for the Sandwich Enzymoimmunoassay of Macromolecular Antigens (1977)

KATO, Kanefusa, HAMAGUCHI, Yoshitaka, OKAWA, Sachiyo, ISHIKAWA, Eiji, KOBAYASHI, Keiko, KATUNUMA, Nobuhiko

A highly sensitive sandwich enzymoimmunoassay of macromolecular antigens using the Fab' of rabbit antibody coupled to -β-D-galactosidase together with rabbit antibody IgG loaded on silicone pieces...

Effects of Estrogen on the Turnover Rates of Ornithine Aminotransferase in Rat Liver and Kidney (1976)

KOBAYASHI, Keiko, KITO, Kiyoaki, KATUNUMA, Nobuhiko

The turnover rates of ornithine aminotransferase in the liver and kidney of control rats and those treated with estrogen were determined by injecting L-[14C]leucine (U) and following the decay of...

Studies on the Turnover Rates of Ornithine Aminotransferase in Morris Hepatoma 44 and Host Liver (1976)

KOBAYASHI, Keiko, MORRIS, Harold P., KATUNUMA, Nobuhiko

The ornithine aminotransferase [EC 2.6.1.13] content of Morris hepatoma 44 is about 15 times higher than that in normal liver. The turnover rates of ornithine aminotransferase in hepatoma 44 and host...

Enzyme-linked Sandwich Immunoassay of Ornithine {delta}-Aminotransferase from Rat Liver Using Antibody-coupled Glass Rods as Solid Phase (1976)

HAMAGUCHI, Yoshitaka, KATO, Kanefusa, FUKUI, Hideo, SHIRAKAWA, Isao, ISHIKAWA, Eiji, KOBAYASHI, Keiko, ...

A macromolecular antigen, ornithine δ-aminotransferase [EC 2. 6.1.13] from rat liver (OAT) was assayed by the sandwich procedure using a rabbit (anti-OAT) Fab'-β-D-galactosidase complex and rabbit...

Comparative Studies on Glutamine, Serine, and Glycine Metabolisms in Ureotelic and Uricotelic Animals (1973)

MATSUDA, Yoshiko, KURODA, Yasuhiro, KOBAYASHI, Keiko, KATUNUMA, Nobuhiko

The mechanisms regulating serine, glycine, and glutamine metabolism were compared in ureotelic animals and uricotelic animals. The activities of five enzymes (glutaminase [EC 3.5.1.2], glutamine...

Characterization of the Enzymatic Component of Clostridium perfringens Iota-Toxin

Nagahama, Masahiro, Sakaguchi, Yoshihiko, Kobayashi, Keiko, Ochi, Sadayuki, Sakurai, Jun

The iotaa component (ia) of Clostridium perfringens ADP ribosylates nonmuscle β/γ actin and skeletal muscle α-actin. Replacement of Arg-295 in ia with alanine led to a complete loss of...

Binding Component of Clostridium perfringens Iota-Toxin Induces Endocytosis in Vero Cells

Nagahama, Masahiro, Nagayasu, Koichi, Kobayashi, Keiko, Sakurai, Jun

Clostridium perfringens iota-toxin is a binary toxin consisting of two individual proteins, the binding component (Ib) and the enzyme component (Ia). Wild-type Ib bound to Vero cells at 4 and 37°C...

MYO18B, a candidate tumor suppressor gene at chromosome 22q12.1, deleted, mutated, and methylated in human lung cancer

Nishioka, Michiho, Kohno, Takashi, Tani, Masachika, Yanaihara, Nozomu, Tomizawa, Yoshio, Otsuka, Ayaka, ...

Loss of heterozygosity on chromosome 22q has been detected in approximately 60% of advanced nonsmall cell lung carcinoma (NSCLC) as well as small cell lung carcinoma (SCLC), suggesting the presence...

Slc25a13-Knockout Mice Harbor Metabolic Deficits but Fail To Display Hallmarks of Adult-Onset Type II Citrullinemia

Sinasac, David S., Moriyama, Mitsuaki, Jalil, M. Abdul, Begum, Laila, Li, Meng Xian, Iijima, Mikio, ...

Adult-onset type II citrullinemia (CTLN2) is an autosomal recessive disease caused by mutations in SLC25A13, the gene encoding the mitochondrial aspartate/glutamate carrier citrin. The absence of...

Binding and Internalization of Clostridium perfringens Iota-Toxin in Lipid Rafts

Nagahama, Masahiro, Yamaguchi, Akiwo, Hagiyama, Tohko, Ohkubo, Noriko, Kobayashi, Keiko, Sakurai, Jun

Clostridium perfringens iota-toxin is a binary toxin composed of an enzymatic component (Ia) and a binding component (Ib). The oligomer of Ib formed in membranes induces endocytosis. We examined the...

Characterization of the Enzymatic Component of Clostridium perfringens Iota-Toxin

Nagahama, Masahiro, Sakaguchi, Yoshihiko, Kobayashi, Keiko, Ochi, Sadayuki, Sakurai, Jun

The iotaa component (ia) of Clostridium perfringens ADP ribosylates nonmuscle β/γ actin and skeletal muscle α-actin. Replacement of Arg-295 in ia with alanine led to a complete loss of...

Binding Component of Clostridium perfringens Iota-Toxin Induces Endocytosis in Vero Cells

Nagahama, Masahiro, Nagayasu, Koichi, Kobayashi, Keiko, Sakurai, Jun

Clostridium perfringens iota-toxin is a binary toxin consisting of two individual proteins, the binding component (Ib) and the enzyme component (Ia). Wild-type Ib bound to Vero cells at 4 and 37°C...

MYO18B, a candidate tumor suppressor gene at chromosome 22q12.1, deleted, mutated, and methylated in human lung cancer

Nishioka, Michiho, Kohno, Takashi, Tani, Masachika, Yanaihara, Nozomu, Tomizawa, Yoshio, Otsuka, Ayaka, ...

Loss of heterozygosity on chromosome 22q has been detected in approximately 60% of advanced nonsmall cell lung carcinoma (NSCLC) as well as small cell lung carcinoma (SCLC), suggesting the presence...

Slc25a13-Knockout Mice Harbor Metabolic Deficits but Fail To Display Hallmarks of Adult-Onset Type II Citrullinemia

Sinasac, David S., Moriyama, Mitsuaki, Jalil, M. Abdul, Begum, Laila, Li, Meng Xian, Iijima, Mikio, ...

Adult-onset type II citrullinemia (CTLN2) is an autosomal recessive disease caused by mutations in SLC25A13, the gene encoding the mitochondrial aspartate/glutamate carrier citrin. The absence of...

Binding and Internalization of Clostridium perfringens Iota-Toxin in Lipid Rafts

Nagahama, Masahiro, Yamaguchi, Akiwo, Hagiyama, Tohko, Ohkubo, Noriko, Kobayashi, Keiko, Sakurai, Jun

Clostridium perfringens iota-toxin is a binary toxin composed of an enzymatic component (Ia) and a binding component (Ib). The oligomer of Ib formed in membranes induces endocytosis. We examined the...

Identification of the Imprinted KLF14 Transcription Factor Undergoing Human-Specific Accelerated Evolution

Parker-Katiraee, Layla, Carson, Andrew R, Yamada, Takahiro, Arnaud, Philippe, Feil, Robert, Abu-Amero, Sayeda N, ...

Imprinted genes are expressed in a parent-of-origin manner and are located in clusters throughout the genome. Aberrations in the expression of imprinted genes on human Chromosome 7 have been...

An Alternative Approach to the Monitoring of Respiration by Dynamic Air-Pressure Sensor

Takarada, Tohru, Kawahara, Michio, Irifune, Masahiro, Endo, Chie, Shimizu, Yoshitaka, Kobayashi, Keiko, ...

Monitoring and assessing of patient respiratory function during conscious sedation are important because many drugs used for conscious sedation produce respiratory depression and subsequent...

Complete blockage of the mevalonate pathway results in male gametophyte lethality

Suzuki, Masashi, Nakagawa, Shoko, Kamide, Yukiko, Kobayashi, Keiko, Ohyama, Kiyoshi, Hashinokuchi, Hiromi, ...

Plants have two isoprenoid biosynthetic pathways: the cytosolic mevalonate (MVA) pathway and the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Since the discovery of the MEP pathway,...